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No products found
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Quote:
Initial crystallization screening was set up by a Mosquito crystallization robot (TTP Labtech) using the sitting-drop vapor-diffusion method at room temperature ...
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No products found
because this supplier's products are not listed.
Quote:
... Initial crystallization conditions were obtained with the Protein Complex Suite (Qiagen) screened by sitting drop-vapor diffusion at 18 °C using a Mosquito robot and were optimized in hanging drops by mixing 1 μL of the sample with 1 μL of the precipitant ...
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No products found
because this supplier's products are not listed.
Yi-Yun Cheng, et al.,
bioRxiv - Biophysics 2018
Quote:
... Crystals were screened either manually or by using the automatic protein crystallization robot Oryx8 (Douglas Instruments, Hungerford, UK) with Wizard crystallization screens (Rigaku, WA, USA). The urea-treated dFABP (denoted as 5GGE ...
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No products found
because this supplier's products are not listed.
Quote:
... Initial crystallization hits were obtained in condition number C4 of the PEG/Ion HT crystallization screen (Hampton Research). The crystallization condition was further optimized using the Additive screen HT (Hampton Research ...
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No products found
because this supplier's products are not listed.
Quote:
Initial crystallization trials were performed by the sitting-drop vapour-diffusion method in a 96-well MRC-crystallization plate (Molecular Dimensions) using a Mosquito (TTP Labtech ...
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No products found
because this supplier's products are not listed.
Raymond K. Yengo, et al.,
bioRxiv - Biophysics 2018
Quote:
... the crystallization Kit for DNA (Sigma Aldrich) but only the most promising were optimized ...
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No products found
because this supplier's products are not listed.
Quote:
... and commercial crystallization kits (MCSG-1-4, Anatrace). The drops were prepared by mixing equal volumes (0.4 μl ...
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No products found
because this supplier's products are not listed.
Quote:
... and 50 mM MES–NaOH (pH 6.5) was placed in a well of a Nunc HLA crystallization plate (Nalge Nunc International) which was then covered with 20 μl of paraffin oil.
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No products found
because this supplier's products are not listed.
Quote:
... along with two 4-point calibration curves consisting of mixed-fibre human muscle lysate with a known protein concentration (0.25 to 3 μg protein) and two protein ladders (Precision Plus Protein All Blue, #1610373, Bio-Rad). Proteins were separated by electrophoresis (45 min at 200V) ...
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No products found
because this supplier's products are not listed.
Quote:
... Proteins were crystallized using a TTP Labtech’s Mosquito LCP crystallization robot and crystallization screens (Jena Bioscience and Quiagen). Conditions leading to crystals were subsequently optimized ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein A and Protein G (GE Healthcare) beads (mixed in a 1:1 ratio ...
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No products found
because this supplier's products are not listed.
Quote:
... HBV E protein (HBe protein) or HBV S protein (HBs protein) (Abcam) at 37°C in a 5% CO2 atmosphere ...
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No products found
because this supplier's products are not listed.
Senén D. Mendoza, et al.,
bioRxiv - Microbiology 2018
Quote:
... Cas9 protein (NEB) was combined with pre-annealed crRNA and tracRNA complex at a 1:1 molar ratio ...
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No products found
because this supplier's products are not listed.
Antonio Boza-Serrano, et al.,
bioRxiv - Neuroscience 2018
Quote:
... PBS and RIPA solution were prepared using a protein inhibitor (Protein Inhibitor Cocktail, ThermoScientific) to prevent protein degradation and to inhibit the enzymatic activity of phosphatases (PhosphoStop, Roche).
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No products found
because this supplier's products are not listed.
Quote:
... followed by salting out of the proteins with Protein Precipitation Solution (Promega, A7951). The genomic DNA was precipitated with isopropanol and resuspended in TE (10mM Tris pH8.0 ...
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No products found
because this supplier's products are not listed.
Rana N. Ozdeslik, et al.,
bioRxiv - Physiology 2019
Quote:
... 15 μl of 50% (w/v) protein A/G or protein A beads (Santa Cruz Biotechnology) were added to the supernatant and rotated for 30 min to preclear the samples ...
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No products found
because this supplier's products are not listed.
Quote:
... protein block (Dako) for 10 minutes at room temperature ...
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No products found
because this supplier's products are not listed.
Tamara Weiss, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Protein extracts were stored in Protein LoBind tubes (Eppendorf) at −80°C ...
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No products found
because this supplier's products are not listed.
Emma J Kelsall, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Protein samples were run on a SDS-PAGE gel and proteins were visualised by InstantBlue protein stain (Expedeon). Quantitation of the intensities of Ras1.G17V (1-172 ...
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No products found
because this supplier's products are not listed.
Tamara M Sirey, et al.,
bioRxiv - Biochemistry 2018
Quote:
... Protein carbonylation was assessed using the OxyBlot protein oxidation detection kit (Merck Millipore), and differential carbonylation was assessed by densitometry ...
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No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
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No products found
because this supplier's products are not listed.
Quote:
... coli protein production (Genscript) and used as templates for subsequent cloning ...
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Quote:
... Proteins were tagged with eGfp (enhanced green fluorescent protein; Clontech, Mountain View, CA, USA), Tag-Rfp or mCherry [26 ...
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No products found
because this supplier's products are not listed.
Quote:
... Purified K-GECO was concentrated to 10 mg/mL for crystallization using centrifugal concentrators (Sartorius Vivaspin, 10,000 MWCO). Purified K-GECO1 protein at 10 mg/mL in 10 mM Tris ...
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No products found
because this supplier's products are not listed.
Justin Parreno, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Total protein of samples loaded into capillaries was determined using a Total Protein Separation module kit (Protein Simple). Protein expression of specific molecules were normalized to total protein.
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No products found
because this supplier's products are not listed.
Dharendra Thapa, et al.,
bioRxiv - Physiology 2019
Quote:
... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein concentration was measured using Precision Red protein assay reagent (Cytoskeleton, Inc.). ∼40 μg lysates were separated by SDS-PAGE and transferred onto the Immobilon P PVDF membrane (EMD Millipore) ...
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No products found
because this supplier's products are not listed.
Nadiia Rawlings, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Total protein on membranes was stained using REVERT total protein stain (LI-COR). Immune complexes were detected using HRP-conjugated secondary antibodies (Sigma ...
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No products found
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Quote:
... recombinant ephrinA4-Fc protein (R&D Systems) was added at the start of the coculture to a final concentration of 2µg/ml.
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No products found
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Quote:
... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...
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Quote:
... We therefore identified the brain development protein-protein interaction network (BD-PPI network) for the 22q11.2 locus (i.e. ...
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No products found
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Quote:
... MS2 coat protein (Addgene #27121) was fused with superfolder GFP (Kersey et al. ...
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No products found
because this supplier's products are not listed.
Quote:
... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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No products found
because this supplier's products are not listed.
Luke J. Fulcher, et al.,
bioRxiv - Cell Biology 2018
Quote:
... RFP (red fluorescent protein) TRAP beads (ChromoTek) were used ...
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No products found
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Quote:
... The solubilized protein in the crystallization buffer was mixed with pre-melted at 42°C monoolein (Nu-Chek Prep) to form a lipidic mesophase ...
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No products found
because this supplier's products are not listed.
Kensuke Ninomiya, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... All MS/MS spectra were searched against protein sequences of the RefSeq (NCBI) human protein database (RDB) using the Protein Pilot software package (AB Sciex), and decoy sequences were then selected with a false discovery rate <1%.
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No products found
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Quote:
... protein A biosensors (Pall) were equilibrated in sample diluent buffer (PBS containing 0.1% BSA and 0.02% Tween-20) ...
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No products found
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Quote:
... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ...
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No products found
because this supplier's products are not listed.
Robyn M. Barfield, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... except that HER2-His protein (Sino Biological) was used as the capture reagent for the total antibody and total ADC measurements.
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No products found
because this supplier's products are not listed.
Lorna Cryan, et al.,
bioRxiv - Biochemistry 2019
Quote:
... matrix protein (Rockland Immunochemicals ...
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No products found
because this supplier's products are not listed.
Juha Auvinen, et al.,
bioRxiv - Epidemiology 2018
Quote:
... and at 5-weeks of age the mice were transferred to Teklad Global 19% Protein Extruded Rodent Diet (19.0% protein, 9.0% fat, 44.9% carbohydrates; Envigo) to slightly support the increase in adipocity upon aging ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein was concentrated (Corning Spin-X concentrators) at 4C before further purification over a Superdex 200 size-exclusion column (GE Healthcare ...
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No products found
because this supplier's products are not listed.
Quote:
... coli NucC bound to 5’-pApA or cAAA in hanging drop format by mixing protein (8-10 mg/mL) in crystallization buffer plus 0.1 mM 5’-pApA (Invivogen) or cAAA 1:1 with well solution containing 17-24% PEG 3350 ...
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No products found
because this supplier's products are not listed.
Quote:
For immunoprecipitation 0.8 μl anti V5 antibody was added to 500 μl cell lysate and incubated over-night on a rotating wheel at 4°C and the protein complex was precipitated by using 50 μl of Protein A and Protein G Plus-Agarose beads (Calbiochem) in EBC buffer ...
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No products found
because this supplier's products are not listed.
Lin Yuan, et al.,
bioRxiv - Immunology 2019
Quote:
... Protein concentration was measured by BCA protein determination kit (PC0020, Solarbio, China). Immunoprecipitations were performed overnight using the appropriate dilution of a polyclonal anti-ITGB4 antibody (Ab197772 ...
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No products found
because this supplier's products are not listed.
Makoto Tanaka, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... we extracted CY and PM proteins using the PM Protein Extraction Kit (BioVision). The protein samples were treated for 30 min at 50°C in a sample-treating solution containing 2% SDS and 5% β-mercaptoethanol ...
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No products found
because this supplier's products are not listed.
Sonia Accossato, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Immunoprecipitation to isolate the protein complexes from total protein extracts using GFP-tagged microbeads (Miltenyi Biotec) has been described previously [8] ...
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No products found
because this supplier's products are not listed.
Quote:
Bio-Rad protein electrophoresis (Mini-PROTEAN® Tetra Cell Systems and TGX™ precast gels ...
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No products found
because this supplier's products are not listed.
Quote:
... and protein purification kit (Macherey-Nagel 740609.240C) following the manufacturers protocol ...
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No products found
because this supplier's products are not listed.
Thomas Mandel Clausen, et al.,
bioRxiv - Microbiology 2020
Quote:
... Bound biotinylated protein was detected by adding Avidin-HRP (405103, BioLegend) diluted 1:2000 in TSM buffer ...
Catalog #: K1010003 Crystallization screen consisting of 48 unique crystallization reagents...Cat# K1010003, USD $695.00/ea Ask