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No products found
because this supplier's products are not listed.
Chih-Chien Sung, et al.,
bioRxiv - Systems Biology 2018
Quote:
... After determining protein concentrations (Bio-Rad Protein Assay Kit), immunoblotting was performed ...
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No products found
because this supplier's products are not listed.
Natalie K. Thulin, et al.,
bioRxiv - Immunology 2019
Quote:
... Protein concentrations were quantified using BCA Protein Assay Kit (ThermoFisher; 23227) and lysates were reduced by boiling for 5 min with Laemmli SDS sample buffer containing 5% β-mercaptoethanol ...
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No products found
because this supplier's products are not listed.
Xue Bai, Chunyang Zheng, Na Ren,
bioRxiv - Cell Biology 2019
Quote:
... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
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No products found
because this supplier's products are not listed.
Jaafar Kilani, et al.,
bioRxiv - Microbiology 2019
Quote:
Protein concentration was measured with the “2D Quant kit” (GE Healthcare Life Sciences), according to the supplier’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein concentrations were determined with a direct detection spectrometer (Merck), and purified proteins were stored at −80°C ...
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No products found
because this supplier's products are not listed.
Ritusree Biswas, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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No products found
because this supplier's products are not listed.
Quote:
... and concentration with the RNeasy MinElute kit (QIAGEN). RNA integrity was assessed with the Agilent 2100 Bioanalyzer and samples were hybridised to Affymetrix Mouse All Exon 1.0 ST microarrays ...
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No products found
because this supplier's products are not listed.
Quote:
... The total protein concentration was determined by using Bradford protein assay kit (Abcam) and the collagen content of each sample was normalized to total protein.
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No products found
because this supplier's products are not listed.
Nuria Estévez-Gómez, et al.,
bioRxiv - Genomics 2018
Quote:
... We quantified library concentration with the Kapa Library Quantification Kit (Kapa Biosystems) for Illumina and the Ion Library Quantitation Kit (Life Technologies ...
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No products found
because this supplier's products are not listed.
Rajalakshmi Srinivasan, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Protein concentrations were quantified using Bicinconinic assay kit (G-Biosciences, 786-570) and equal concentrations of proteins were loaded into the 4-12% Bis-tris polyacrylamide gel (Thermo Fisher,NP0322BOX ...
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No products found
because this supplier's products are not listed.
Quote:
... Lactis Protein Expression kit (New England Biolabs, UK) was used with the pKLAC2 vector ...
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No products found
because this supplier's products are not listed.
Quote:
... Lysates were spun and protein concentrations measured using a BCA protein assay kit (BioVision). After adjusting protein concentrations ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein concentration was measured using Precision Red protein assay reagent (Cytoskeleton, Inc.). ∼40 μg lysates were separated by SDS-PAGE and transferred onto the Immobilon P PVDF membrane (EMD Millipore) ...
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No products found
because this supplier's products are not listed.
Quote:
... and concentration determined using 2100 Bioanalyzer with High Sensitivity DNA kit (Agilent). Libraries were sequenced on HiS-eq 1500 (Illumina ...
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No products found
because this supplier's products are not listed.
Murtala Muhammad, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Protein purity and concentration were determined by SDS-PAGE and BCA protein assay kit (Takara) respectively ...
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No products found
because this supplier's products are not listed.
Alejandra Arias-Cavieres, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Protein content was determined using a Protein Determination Kit (Cayman Chemical, Cat# 704002) and absorbance was measured at 595 nm using a Nanodrop 2000 spectrophotometer (Thermofisher ...
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No products found
because this supplier's products are not listed.
Lin Yuan, et al.,
bioRxiv - Immunology 2019
Quote:
... Protein concentration was measured by BCA protein determination kit (PC0020, Solarbio, China). Immunoprecipitations were performed overnight using the appropriate dilution of a polyclonal anti-ITGB4 antibody (Ab197772 ...
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No products found
because this supplier's products are not listed.
Sagar R. Shah, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Supernatant protein concentrations were quantified using a BCA Protein Assay kit (Pierce) and a spectrophotometric plate reader (BioTek). Most protein lysates were separated by running 30-100 μg of lysate on 10% Bis-Tris NuPage gels (Invitrogen ...
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No products found
because this supplier's products are not listed.
Quote:
... and the RNA Clean and Concentration Kit (Zymo Resaearch). Libraries were generated ...
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No products found
because this supplier's products are not listed.
Brayon J. Fremin, Ami S. Bhatt,
bioRxiv - Microbiology 2018
Quote:
... The concentration was determined with Qubit RNA HS Assay Kit (Illumina). With 100 ng as input ...
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No products found
because this supplier's products are not listed.
Qinlin Shi, et al.,
bioRxiv - Immunology 2020
Quote:
... and the protein concentration was measured by the BCA protein assay kit (Genscript, Nanjing, China) as previous described25.
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No products found
because this supplier's products are not listed.
H. Tran, L. Anderson, J. Knight,
bioRxiv - Biophysics 2018
Quote:
... protein concentrations were measured using UV absorbance (PerkinElmer) based on predicted extinction coefficients at 280 nm (http://protcalc.sourceforge.net) ...
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No products found
because this supplier's products are not listed.
Melanie V. Halbach, et al.,
bioRxiv - Neuroscience 2018
Quote:
... Protein concentration was determined with a Spectrophotometer (Eppendorf) using 5x Bradford Reagent (Roti-Quant ...
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No products found
because this supplier's products are not listed.
Quote:
... cells were incubated with titrated concentrations of human TGF-β1 protein (R&D systems) in the complete culture medium ...
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No products found
because this supplier's products are not listed.
Pratikshya Ray, Palok Aich,
bioRxiv - Microbiology 2019
Quote:
... Protein concentration was normalized through Bradford assay (BD Biosciences, San Diego, CA, USA). Absorbance was taken through Multiskan Go (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Xin Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Protein concentration was measured using Bradford protein assay kit (Tiangen Biotech, Beijing Co., LTD, CN). The purity of the PM was determined by standard marker assays[32] ...
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No products found
because this supplier's products are not listed.
Quote:
... Endotoxin concentrations were measured in all protein samples using the Limulus Amebocyte Lysate Kit – QCL-1000 (Lonza, Basel, Switzerland). The rMIC1 ...
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No products found
because this supplier's products are not listed.
Yu-Jung Chang, Kuan-Wei Chen, Linyi Chen,
bioRxiv - Cancer Biology 2020
Quote:
... Protein concentrations were determined by the bicinchoninic acid assay (Santa Cruz Biotechnology), and were separated by SDS-PAGE ...
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No products found
because this supplier's products are not listed.
Veronika Kana, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Supernatants containing 70 µg of total protein were used to determine IL-34 concentration using an IL-34 ELISA kit (BioLegend, San Diego, CA).
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No products found
because this supplier's products are not listed.
Quote:
... using a FASP™ Protein Digestion Kit (Expedeon). The resulting peptides were desalted and analyzed on the LC-MS instrument (Q-Exactive Plus ...
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No products found
because this supplier's products are not listed.
Quote:
... The protein concentration in tissue lysates was measured with a BCA protein assay kit (Boster, Wuhan, China) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... and protein concentration was equalised before immunoprecipitation with GFP-TRAP (ChromoTek) beads according to the manufacturer’s protocol.
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No products found
because this supplier's products are not listed.
Pierre Gillotay, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... the protein Cas9 (recombinant cas protein from S. pyogenes PNA Bio CP01, final concentration 100 ng/μL) and KCL (final concentration 200 mM) ...
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No products found
because this supplier's products are not listed.
Quote:
... and protein purification kit (Macherey-Nagel 740609.240C) following the manufacturers protocol ...
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No products found
because this supplier's products are not listed.
Quote:
The μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) was used to pull down proteins associated with the GFP-tagged protein ...
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No products found
because this supplier's products are not listed.
Laura Cheradame, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Cell lysate protein concentration was determined using a Micro BCA Protein Assay kit (Bio Basic). Unless specified ...
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No products found
because this supplier's products are not listed.
Quote:
... Known concentrations of purified fluorescent protein standards (GFP: Rockland 000-001-215 lot 23193 ...
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No products found
because this supplier's products are not listed.
Quote:
... The total protein concentration was determined using a SpectraMax® Plus 384 (Molecular Devices) spectrophotometer with a 562 nm filter using the Pierce™ BCA Protein Assay kit (Thermo Scientific ...
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No products found
because this supplier's products are not listed.
Justin Parreno, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Total protein of samples loaded into capillaries was determined using a Total Protein Separation module kit (Protein Simple). Protein expression of specific molecules were normalized to total protein.
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No products found
because this supplier's products are not listed.
Mariana G Bego, et al.,
bioRxiv - Immunology 2019
Quote:
... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Mootaz Salman, et al.,
bioRxiv - Bioengineering 2020
Quote:
... A hydrogel consisting of extracellular matrix (ECM) proteins made of a final concentration of 7.0 mg/ml Type I rat tail collagen (Corning, 354249, MA, USA) was used in all experiments ...
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No products found
because this supplier's products are not listed.
Yuka Yashima, et al.,
bioRxiv - Cell Biology 2018
Quote:
... The amount of protein concentration of RANKL (Murine sRANK Ligand Mini ABTS ELISA Development kit, PeproTech Inc, Rocky Hill, NJ, USA), OPG (Osteoprotegerin Mouse Immunoassay kit ...
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No products found
because this supplier's products are not listed.
Quote:
... Plasma human IgG concentration was determined by ELISA kit (Bethyl Laboratories ...
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No products found
because this supplier's products are not listed.
Celeste M. Sheftel, Laura L. Hernandez,
bioRxiv - Molecular Biology 2020
Quote:
Intracellular 5HT concentrations were determined using a Serotonin Enzyme Immunoassay Kit (Beckman Coulter ...
-
No products found
because this supplier's products are not listed.
Quote:
... and concentrated to 20 uL using the RNA Clean-Up and Concentration Kit (Norgen Biotek). To limit sample variation between MAD-DASH replicate groups ...
-
No products found
because this supplier's products are not listed.
Tatiana M. Garcia-Bates, et al.,
bioRxiv - Immunology 2020
Quote:
... soluble recombinant human CD40L was added at a concentration of 0.25 μg/ml (MEGACD40L® protein, ENZO Life Sciences, Farmingdale, NY). After 4 to 5 d stimulation ...
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No products found
because this supplier's products are not listed.
Nehemiah S Alvarez,
bioRxiv - Molecular Biology 2019
Quote:
... 30 µL magnetic protein G beads (SimpleChIP Kit #9003, Cell Signaling Technology) were added to the samples and incubated at 4 °C with end over end rotation for 4 hours ...
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No products found
because this supplier's products are not listed.
Quote:
... the immunoreactive protein was visualized using the Vectastain ABC kit (Vector Laboratories) and 3,3’ diaminobenzidine tetrahydrochloride hydrate (Sigma ...
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No products found
because this supplier's products are not listed.
Quote:
... or 0.1M glycine and precipitated using the Proteo Extract Protein Precipitation Kit (Calbiochem 539180). The dry pellet was submitted to the Taplin Mass Spectrometry Facility (Harvard Medical School ...
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No products found
because this supplier's products are not listed.
Quote:
... after which increasing concentrations of DNQX (Tocris) were added sequentially to the ACSF (approximately 30 minutes/each DNQX concentration ...