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No products found
because this supplier's products are not listed.
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Human ventricular cardiac fibroblasts (Lonza cat# CC-2904) were grown in 1% gelatin coated flasks in DMEM (ThermoFisher cat#11885084 ...
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No products found
because this supplier's products are not listed.
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Primary neonatal human dermal fibroblasts (nHDF; Thermo Fisher Scientific), MRC-5 fibroblasts (American Type Culture Collection ...
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No products found
because this supplier's products are not listed.
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Human cardiac fibroblasts (hCF) were purchased commercially (PromoCell) and maintained in Advanced DMEM (Life Technologies ...
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No products found
because this supplier's products are not listed.
Quote:
... were purchased from Cellular Dynamics and Human Cardiac Fibroblasts (HCFs) (6300) were purchased from Sciencell Research Laboratories ...
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No products found
because this supplier's products are not listed.
Elena Tsolaki, et al.,
bioRxiv - Pathology 2019
Quote:
... Human dermal fibroblast cells (HFC, primary cells, Sigma Aldrich, Buchs, Switzerland) were cultivated in DMEM supplemented with 15% FCS and 1% PSN/L-glutamine ...
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No products found
because this supplier's products are not listed.
Tracy A. Hookway, et al.,
bioRxiv - Bioengineering 2020
Quote:
... and fed with Cardiac Fibroblast Medium (Cell Applications) every 2-3 days ...
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No products found
because this supplier's products are not listed.
Quote:
... cardiac fibroblasts were pre-treated in plastic tissue-culture flasks with 10 nM human TGF-β1 (Cell Signaling Technology, cat# 8915LC) for 48h ...
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No products found
because this supplier's products are not listed.
Quote:
... Cardiac fibroblasts and HUVECs were plated in clear 96-well plates (Corning) and HEK T-REx-293 cells in black ...
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No products found
because this supplier's products are not listed.
Jeffrey Harding, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Immunohistochemistry was performed to stain cells with the primary anti-cardiac Troponin-T antibody (abcam, ab8295) and detected with secondary fluorophore-conjugated antibody against Goat IgG (abcam ab205719).
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No products found
because this supplier's products are not listed.
Hongxing Zhao, et al.,
bioRxiv - Microbiology 2018
Quote:
Human primary lung fibroblast IMR-90 cells (American Type Culture Collection, ATCC) were initially cultured in Eagle’s minimum essential medium (EMEM ...
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No products found
because this supplier's products are not listed.
Quote:
... Human fetal cardiac fibroblasts (CFs) were obtained from Cell Biologics, Cat # H6049 (Chicago ...
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No products found
because this supplier's products are not listed.
Vadim Le Joncour, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... 0.01 μg/ml human fibroblast growth factor (FGF, Peprotech), 0.02 μ/ml human epidermal growth factor (EGF ...
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No products found
because this supplier's products are not listed.
Quote:
... Recombinant human fibroblast growth factor basic was purchased from R&D Systems, Inc ...
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No products found
because this supplier's products are not listed.
Dan Sarni, et al.,
bioRxiv - Genetics 2019
Quote:
Human foreskin fibroblasts BJ-hTERT cells (Clontech) were grown in DMEM supplemented with 10% fetal bovine serum ...
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No products found
because this supplier's products are not listed.
Jacob Lamberth, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Human primary CD8+ T cells were purchased from STEMCELL Technologies Inc ...
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No products found
because this supplier's products are not listed.
Shannon P Hilchey, et al.,
bioRxiv - Immunology 2019
Quote:
... Primary human B cells: Human peripheral blood was collected by routine phlebotomy in heparinized vacutainers (BD Biosciences, San Diego, CA) and diluted 1:2 with PBS containing 2% FBS ...
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No products found
because this supplier's products are not listed.
Quote:
The mitochondrial oxygen consumption rate (OCR) in both primary neurons and human fibroblasts were assessed using a Seahorse Bioscience XFe24 analyzer (Agilent, Massachusetts, USA) in combination with the Seahorse Bioscience XF Cell Mito Stress Test assay kit ...
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No products found
because this supplier's products are not listed.
Quote:
Primary cardiac cultures were isolated from P7 mice using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) using the gentleMACS homogenizer ...
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No products found
because this supplier's products are not listed.
Gary LeRoy, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... 2.5ng/ml basic human fibroblast growth factor (Promega), 1X GlutaMax (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Moeed Akbar, et al.,
bioRxiv - Immunology 2018
Quote:
... Fibroblasts were then stimulated with 10ng/ml recombinant human IL-1β (Biolegend) for 24 hours in complete RPMI.
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No products found
because this supplier's products are not listed.
Quote:
Total RNA was extracted from human and mouse lung fibroblasts using RNeasy columns (Qiagen) and quantified using Qubit RNA high sensitivity assay kit (Life Technologies) ...
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No products found
because this supplier's products are not listed.
Cristina M. Failla, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Primary antibodies (anti-human IL-17RA antibody, Cell Signaling Technology, Danvers, MA, USA; anti-human IL-1Rrp2 antibody, Santa Cruz Biotechnologies, Santa Cruz, USA) were used diluted 1:1000 and applied for 18 hours followed by the appropriate horseradish peroxidase-coupled secondary antibody (GE Bioscience).
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No products found
because this supplier's products are not listed.
Quote:
... Recombinant human fibroblast growth factor 1 (GF002) and fibroblast growth factor 2 (GF003) were purchased from Merck.
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No products found
because this supplier's products are not listed.
Quote:
... Transfection in MRC5-SV40 transformed human fibroblasts was performed using Fugene transfection reagent (Roche). Stably expressing cells were isolated after selection with G418 (Gibco ...
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No products found
because this supplier's products are not listed.
Quote:
IM fibroblasts were transfected either with human H2B-GFP (from Geoff Wahl lab, Addgene plasmid # 11680) or pSV-IRESneo3-CENP-A-EGFP (kind gift from Patrick Meraldi ...
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No products found
because this supplier's products are not listed.
Quote:
... the human β-cardiac sS1 was further purified using anion exchange chromatography on a 1 mL HiTrap Q HP column (GE Healthcare). Peak fractions were eluted with column buffer (10 mM Imidazole ...
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No products found
because this supplier's products are not listed.
Allison Ogren, et al.,
bioRxiv - Genetics 2019
Quote:
... we obtained a primary neonatal cardiomyocyte cell culture derived from normally developing human neonatal cardiac tissue from Celprogen (San Pedro, CA Cat#36044-21). These cells were also grown in DMEM plus 10% fetal calf serum and 1% penn/strep.
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No products found
because this supplier's products are not listed.
Quote:
Human induced pluripotent stem cell derived cardiac myocytes (iPSC CMs) were purchased (Cellular Dynamics International, USA) and stored in liquid nitrogen until use ...
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ACBRI 5118 cells were isolated by centrifugal counterflow elutriation from dissociated normal...
Cat# ACBRI 5118 V,
Frozen Vial with 1E6 cells, USD $735.0/1ea
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Quote:
Primary human retinal microvascular endothelial cells (HRECs) were obtained from Cell Systems, Inc ...
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No products found
because this supplier's products are not listed.
Quote:
Primary mouse lung fibroblasts were seeded in 96-well black CellCarrier (PerkinElmer) plates and transfected with 12.5 nM On-Targetplus siRNAs to Il11 or Il11ra1 (Dharmacon ...
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No products found
because this supplier's products are not listed.
Quote:
... from primary fibroblasts of affected and control individuals were loaded onto Mini-PROTEAN TGX gels (BIO-RAD). Immunofluorescence assays were performed using primary fibroblasts from patient and control individuals grown in Nunc Lab-Tek chamber slides (Thermo Scientific ...
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No products found
because this supplier's products are not listed.
Quote:
... Primary cancer associated fibroblasts (CAFs) (52) were infected with a pBABE retroviral vector (Cell Biolabs, Bio-Connect Bv ...
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No products found
because this supplier's products are not listed.
L Miyashita, G Foley, S Semple, J Grigg,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
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No products found
because this supplier's products are not listed.
Quote:
... as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch, 1:2000) or goat anti-rabbit IgG-HRP (Abcam # ab6721 ...
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No products found
because this supplier's products are not listed.
Quote:
... Anti-ELP4 (rabbit anti-human primary, CAT# NBP2-16322) antibody was purchased from Novus Biologicals. Monoclonal anti-β-actin (mouse anti-human primary ...
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No products found
because this supplier's products are not listed.
Mengjia Song, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... the following primary antibodies were used: rabbit anti-human IFN-γ (Proteintech Group, Chicago, Illinois, USA, 1:100), rabbit anti-human ICAM1 (Proteintech Group ...
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No products found
because this supplier's products are not listed.
Quote:
Primary CMs and adherent cardiac cells (ACCs) were isolated from two-day old (p2) Sprague-Dawley neonatal rat (Charles River) hearts ...
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No products found
because this supplier's products are not listed.
Quote:
... human fibroblasts were seeded in µ-Slides (Ibidi GmbH, Martinsried, Germany) containing carbon-coated sapphire discs (Engineering Office M ...
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No products found
because this supplier's products are not listed.
Karina Cuevas-Mora, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Swathi Balaji, et al.,
bioRxiv - Physiology 2019
Quote:
Primary adult (8-10 week old) murine dermal fibroblasts were isolated from the skin of C57BL/6J mice (Jackson Laboratories, Bar Harbor, ME, USA) using standard isolation protocols.57 The fibroblast culture was routinely maintained at 37°C under 5% CO2 in a humidified chamber and cultured in Dulbecco’s Modified Eagle’s media (DMEM ...
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No products found
because this supplier's products are not listed.
Xi Wang, et al.,
bioRxiv - Immunology 2020
Quote:
... Fibroblasts were then transfected via LyoVec™ (Invivogen)/RNA complexes ...
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No products found
because this supplier's products are not listed.
Maud de Dieuleveult, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells were incubated with primary anti-USP9X antibody (Bethyl Laboratories ...
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No products found
because this supplier's products are not listed.
Yu-Chih Tsai, et al.,
bioRxiv - Genomics 2017
Quote:
Human genomic DNA extracted from HEK-293 cells (GenScript) was used for protocol development and protocol optimization experiments ...
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No products found
because this supplier's products are not listed.
SE Eckert, et al.,
bioRxiv - Genomics 2016
Quote:
DNA from CMV strain Merlin grown in fibroblast cell culture was subjected to PreCR (#M0309, New England Biolabs, Ipswich MA, USA) enzymatic repair according to the manufacturer’s recommendations after shearing ...
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No products found
because this supplier's products are not listed.
Julia Franzen, et al.,
bioRxiv - Genomics 2018
Quote:
We compiled 64 available DNAm datasets of untreated primary cells with reliable information on passage numbers (all Illumina 450k Methylation BeadChip ...
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No products found
because this supplier's products are not listed.
Heidi L. H. Malaby, et al.,
bioRxiv - Cell Biology 2018
Quote:
... Primary antibodies against human anti-centromere protein (ACA) (Antibodies Incorporated) were used at 2.5 μg/mL overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Quote:
... the embryos expressing the cardiac mcherry fluorescence reporter were selected under Leica M165 FC microscope (Leica). At 3 dpf ...
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No products found
because this supplier's products are not listed.
Quote:
... The remaining blood collected from cardiac puncture was placed in MiniCollect K2E K2EDTA 0.5ml (Greiner Bio-One #450480). Manufacturer’s instructions were followed to collect the resulting plasma ...
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No products found
because this supplier's products are not listed.
Quote:
... cells were incubated with PE-conjugated mouse anti-human IgG Fc (Southern Biotech) diluted 1:200 at 4°C for 30 minutes ...
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No products found
because this supplier's products are not listed.
Quote:
Human LRRC8A (Origene #RC208632) was cloned with a C-terminal FLAG-tag (DYKDDDDK ...