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Primary Human Cardiac Fibroblast Cells
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Cell Systems Sponsored
ACBRI 5118 cells were isolated by centrifugal counterflow elutriation from dissociated normal...Cat# ACBRI 5118 V, Frozen Vial with 1E6 cells, USD $735.0/1ea Ask
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Lonza
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019, published in Biomaterials doi: 10.1016/j.biomaterials.2019.119741Quote: Human ventricular cardiac fibroblasts (Lonza cat# CC-2904) were grown in 1% gelatin coated flasks in DMEM (ThermoFisher cat#11885084 ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Journal of Virology doi: 10.1128/jvi.00606-18Quote: Primary neonatal human dermal fibroblasts (nHDF; Thermo Fisher Scientific), MRC-5 fibroblasts (American Type Culture Collection ... -
PromoCell
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019, published in Scientific Reports doi: 10.1038/s41598-020-62955-3Quote: Human cardiac fibroblasts (hCF) were purchased commercially (PromoCell) and maintained in Advanced DMEM (Life Technologies ... -
Sciencell Research Laboratories
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2017, published in Scientific Reports doi: 10.1038/s41598-017-17813-0Quote: ... were purchased from Cellular Dynamics and Human Cardiac Fibroblasts (HCFs) (6300) were purchased from Sciencell Research Laboratories ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Pathology 2019Quote: ... Human dermal fibroblast cells (HFC, primary cells, Sigma Aldrich, Buchs, Switzerland) were cultivated in DMEM supplemented with 15% FCS and 1% PSN/L-glutamine ... -
Cell Applications
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: ... and fed with Cardiac Fibroblast Medium (Cell Applications) every 2-3 days ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019, published in Biomaterials doi: 10.1016/j.biomaterials.2019.119741Quote: ... cardiac fibroblasts were pre-treated in plastic tissue-culture flasks with 10 nM human TGF-β1 (Cell Signaling Technology, cat# 8915LC) for 48h ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019Quote: ... Immunohistochemistry was performed to stain cells with the primary anti-cardiac Troponin-T antibody (abcam, ab8295) and detected with secondary fluorophore-conjugated antibody against Goat IgG (abcam ab205719). -
Corning
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Journal of Biological Chemistry doi: 10.1074/jbc.RA119.009167Quote: ... Cardiac fibroblasts and HUVECs were plated in clear 96-well plates (Corning) and HEK T-REx-293 cells in black ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: Human primary lung fibroblast IMR-90 cells (American Type Culture Collection, ATCC) were initially cultured in Eagle’s minimum essential medium (EMEM ... -
Cell Biologics
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in ACS Omega doi: 10.1021/acsomega.9b02811Quote: ... Human fetal cardiac fibroblasts (CFs) were obtained from Cell Biologics, Cat # H6049 (Chicago ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018Quote: ... 0.01 μg/ml human fibroblast growth factor (FGF, Peprotech), 0.02 μ/ml human epidermal growth factor (EGF ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019, published in Scientific Reports doi: 10.1038/s41598-019-53070-zQuote: ... Recombinant human fibroblast growth factor basic was purchased from R&D Systems, Inc ... -
Takara Bio
No products found because this supplier's products are not listed.Cited in Replication Timing and Transcription Identifies a Novel Fragility Signature Under Replication StressbioRxiv - Genetics 2019Quote: Human foreskin fibroblasts BJ-hTERT cells (Clontech) were grown in DMEM supplemented with 10% fetal bovine serum ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: Human primary CD8+ T cells were purchased from STEMCELL Technologies Inc ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... Primary human B cells: Human peripheral blood was collected by routine phlebotomy in heparinized vacutainers (BD Biosciences, San Diego, CA) and diluted 1:2 with PBS containing 2% FBS ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018, published in The Journal of Cell Biology doi: 10.1083/jcb.201806197Quote: The mitochondrial oxygen consumption rate (OCR) in both primary neurons and human fibroblasts were assessed using a Seahorse Bioscience XFe24 analyzer (Agilent, Massachusetts, USA) in combination with the Seahorse Bioscience XF Cell Mito Stress Test assay kit ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020, published in Nature Cell Biology doi: 10.1038/s41556-020-00588-4Quote: Primary cardiac cultures were isolated from P7 mice using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) using the gentleMACS homogenizer ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: ... 2.5ng/ml basic human fibroblast growth factor (Promega), 1X GlutaMax (Thermo Fisher ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... Fibroblasts were then stimulated with 10ng/ml recombinant human IL-1β (Biolegend) for 24 hours in complete RPMI. -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018, published in Science Translational Medicine doi: 10.1126/scitranslmed.aaw1237Quote: Total RNA was extracted from human and mouse lung fibroblasts using RNeasy columns (Qiagen) and quantified using Qubit RNA high sensitivity assay kit (Life Technologies) ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... Primary antibodies (anti-human IL-17RA antibody, Cell Signaling Technology, Danvers, MA, USA; anti-human IL-1Rrp2 antibody, Santa Cruz Biotechnologies, Santa Cruz, USA) were used diluted 1:1000 and applied for 18 hours followed by the appropriate horseradish peroxidase-coupled secondary antibody (GE Bioscience). -
Merck
No products found because this supplier's products are not listed.Cited in FGFR4 phosphorylates MST1 to confer breast cancer cells resistance to MST1/2-dependent apoptosisbioRxiv - Cancer Biology 2018, published in Cell Death & Differentiation doi: 10.1038/s41418-019-0321-xQuote: ... Recombinant human fibroblast growth factor 1 (GF002) and fibroblast growth factor 2 (GF003) were purchased from Merck. -
Roche
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Molecular and Cellular Biology doi: 10.1128/mcb.00225-18Quote: ... Transfection in MRC5-SV40 transformed human fibroblasts was performed using Fugene transfection reagent (Roche). Stably expressing cells were isolated after selection with G418 (Gibco ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Current Biology doi: 10.1016/j.cub.2018.03.023Quote: IM fibroblasts were transfected either with human H2B-GFP (from Geoff Wahl lab, Addgene plasmid # 11680) or pSV-IRESneo3-CENP-A-EGFP (kind gift from Patrick Meraldi ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2018, published in Nature Structural & Molecular Biology doi: 10.1038/s41594-018-0069-xQuote: ... the human β-cardiac sS1 was further purified using anion exchange chromatography on a 1 mL HiTrap Q HP column (GE Healthcare). Peak fractions were eluted with column buffer (10 mM Imidazole ... -
Celprogen
No products found because this supplier's products are not listed.bioRxiv - Genetics 2019Quote: ... we obtained a primary neonatal cardiomyocyte cell culture derived from normally developing human neonatal cardiac tissue from Celprogen (San Pedro, CA Cat#36044-21). These cells were also grown in DMEM plus 10% fetal calf serum and 1% penn/strep. -
Cellular Dynamics
No products found because this supplier's products are not listed.bioRxiv - Physiology 2019, published in Scientific Reports doi: 10.1038/s41598-019-42592-1Quote: Human induced pluripotent stem cell derived cardiac myocytes (iPSC CMs) were purchased (Cellular Dynamics International, USA) and stored in liquid nitrogen until use ... -
Cell Systems
ACBRI 5118 cells were isolated by centrifugal counterflow elutriation from dissociated normal...Cat# ACBRI 5118 V, Frozen Vial with 1E6 cells, USD $735.0/1ea AskbioRxiv - Pharmacology and Toxicology 2018, published in Journal of Pharmacology and Experimental Therapeutics doi: 10.1124/jpet.118.248088Quote: Primary human retinal microvascular endothelial cells (HRECs) were obtained from Cell Systems, Inc ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018, published in Science Translational Medicine doi: 10.1126/scitranslmed.aaw1237Quote: Primary mouse lung fibroblasts were seeded in 96-well black CellCarrier (PerkinElmer) plates and transfected with 12.5 nM On-Targetplus siRNAs to Il11 or Il11ra1 (Dharmacon ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Genetics 2019, published in EMBO Molecular Medicine doi: 10.15252/emmm.201911861Quote: ... from primary fibroblasts of affected and control individuals were loaded onto Mini-PROTEAN TGX gels (BIO-RAD). Immunofluorescence assays were performed using primary fibroblasts from patient and control individuals grown in Nunc Lab-Tek chamber slides (Thermo Scientific ... -
Cell Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Biology of the Cell doi: 10.1111/boc.201800007Quote: ... Primary cancer associated fibroblasts (CAFs) (52) were infected with a pBABE retroviral vector (Cell Biolabs, Bio-Connect Bv ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020, published in Journal of Proteome Research doi: 10.1021/acs.jproteome.0c00521Quote: ... as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch, 1:2000) or goat anti-rabbit IgG-HRP (Abcam # ab6721 ... -
Novus Biologicals
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020, published in Science Signaling doi: 10.1126/scisignal.abb9820Quote: ... Anti-ELP4 (rabbit anti-human primary, CAT# NBP2-16322) antibody was purchased from Novus Biologicals. Monoclonal anti-β-actin (mouse anti-human primary ... -
Proteintech
No products found because this supplier's products are not listed.Cited in Low-dose IFN-γ induces tumor cell stemness in tumor microenvironment of non-small cell lung cancerbioRxiv - Cancer Biology 2019Quote: ... the following primary antibodies were used: rabbit anti-human IFN-γ (Proteintech Group, Chicago, Illinois, USA, 1:100), rabbit anti-human ICAM1 (Proteintech Group ... -
Charles River Labs
No products found because this supplier's products are not listed.Cited in Glial cells in the heart? Replicating the diversity of the myocardium with low-cost 3D modelsbioRxiv - Bioengineering 2018, published in Biofabrication doi: 10.1088/1758-5090/ab4c0aQuote: Primary CMs and adherent cardiac cells (ACCs) were isolated from two-day old (p2) Sprague-Dawley neonatal rat (Charles River) hearts ... -
ibidi
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in mBio doi: 10.1128/mBio.02110-19Quote: ... human fibroblasts were seeded in µ-Slides (Ibidi GmbH, Martinsried, Germany) containing carbon-coated sapphire discs (Engineering Office M ... -
Active Motif
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Physiology 2019Quote: Primary adult (8-10 week old) murine dermal fibroblasts were isolated from the skin of C57BL/6J mice (Jackson Laboratories, Bar Harbor, ME, USA) using standard isolation protocols.57 The fibroblast culture was routinely maintained at 37°C under 5% CO2 in a humidified chamber and cultured in Dulbecco’s Modified Eagle’s media (DMEM ... -
Invivogen
No products found because this supplier's products are not listed.Cited in MDA5 is an essential vita-PAMP sensor necessary for host resistance against Aspergillus fumigatusbioRxiv - Immunology 2020Quote: ... Fibroblasts were then transfected via LyoVec™ (Invivogen)/RNA complexes ... -
Bethyl
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with primary anti-USP9X antibody (Bethyl Laboratories ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Genomics 2017Quote: Human genomic DNA extracted from HEK-293 cells (GenScript) was used for protocol development and protocol optimization experiments ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Genomics 2016Quote: DNA from CMV strain Merlin grown in fibroblast cell culture was subjected to PreCR (#M0309, New England Biolabs, Ipswich MA, USA) enzymatic repair according to the manufacturer’s recommendations after shearing ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Genomics 2018Quote: We compiled 64 available DNAm datasets of untreated primary cells with reliable information on passage numbers (all Illumina 450k Methylation BeadChip ... -
Antibodies Incorporated
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... Primary antibodies against human anti-centromere protein (ACA) (Antibodies Incorporated) were used at 2.5 μg/mL overnight at 4°C ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2017, published in Biology Open doi: 10.1242/bio.030254Quote: ... the embryos expressing the cardiac mcherry fluorescence reporter were selected under Leica M165 FC microscope (Leica). At 3 dpf ... -
Greiner
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2019, published in BMC Biology doi: 10.1186/s12915-020-00780-wQuote: ... The remaining blood collected from cardiac puncture was placed in MiniCollect K2E K2EDTA 0.5ml (Greiner Bio-One #450480). Manufacturer’s instructions were followed to collect the resulting plasma ... -
SouthernBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020, published in Virology doi: 10.1016/j.virol.2020.07.009Quote: ... cells were incubated with PE-conjugated mouse anti-human IgG Fc (Southern Biotech) diluted 1:200 at 4°C for 30 minutes ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in eLife doi: 10.7554/eLife.38461Quote: Human LRRC8A (Origene #RC208632) was cloned with a C-terminal FLAG-tag (DYKDDDDK ...