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Primary Human Aortic Smooth Muscle Cells
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Cell Systems Sponsored
ACBRI 716 cells were isolated by centrifugal counterflow elutriation from dissociated normal...Cat# ACBRI 716, Frozen Vial with 1E6 cells, USD $735.0/1ea Ask
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Lonza
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... Human aortic smooth muscle cells (SMC) were purchased from Lonza Group Ltd and cultured using SmGM™-2 BulletKit™ (Lonza Group Ltd.) ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Physiology 2018Quote: ... or smooth muscle specific anti-smooth muscle actin (Sigma, St. Louis, MO; A2547) primary antibodies ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... Primary antibodies were anti-α-smooth muscle actin (Cat# ab5694, Abcam), anti-MRTF/Mkl1 (Cat# ab49311 ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2016Quote: ... Smooth Muscle Actin (Thermo Fisher, 4A4, 1:500), GFAP (Antagene ... -
PromoCell
No products found because this supplier's products are not listed.bioRxiv - Physiology 2020Quote: ... in smooth muscle cell growth medium 2 (SMCGM2, PromoCell, C-22062), as previously described (Wojciak-Stothard et al. ... -
Sciencell Research Laboratories
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: Primary human pulmonary artery adventitial fibroblasts (hPAFib) and smooth muscle cells (hPASMC) were obtained from ScienCell Research Laboratories (#3120 and #3110 ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in Cardiovascular Toxicology doi: 10.1007/s12012-019-09532-xQuote: ... smooth muscle α-actin (DAKO, M0851) 1:100 ... -
Cell Applications
No products found because this supplier's products are not listed.Cited in The Adaptor Protein Nck1, but not Nck2, Mediates Shear Stress-Induced Endothelial PermeabilitybioRxiv - Molecular Biology 2019Quote: Human aortic endothelial cells (HAECs, CELL Applications) were maintained in MCDB131 containing 10% (v/v ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... Primary antibodies (anti-human IL-17RA antibody, Cell Signaling Technology, Danvers ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: Human aortic endothelia cells (HAEC, 1×105 cells/well) were seeded on Matrigel (Corning) in a 24-well plate and treated with or without 1 μg EV from different groups of Givi-MPC ... -
Santa Cruz
No products found because this supplier's products are not listed.Cited in Protect TUDCA stimulated CKD-derived hMSCs against the CKD-Ischemic disease via upregulation of PrPCbioRxiv - Cell Biology 2018Quote: ... and α-SMA (alpha-smooth muscle actin; Santa Cruz Biotechnology), followed by secondary antibodies conjugated with Alexa Fluor 488 or 594 (Thermo Fisher Scientific) ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018, published in Arteriosclerosis, Thrombosis, and Vascular Biology doi: 10.1161/ATVBAHA.119.312493Quote: ... smooth muscle (~200) and endothelial cells (~100) using the RNeasy plus micro kit (Qiagen) according to instructions ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2017, published in Aging doi: 10.18632/aging.101231Quote: ... mouse anti-smooth muscle actin (SMA, #MAB1420, R&D systems). Cells were then immunostained with the appropriate conjugated secondary antibodies (Alexa Fluor 488 ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... Primary human B cells: Human peripheral blood was collected by routine phlebotomy in heparinized vacutainers (BD Biosciences, San Diego, CA) and diluted 1:2 with PBS containing 2% FBS ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: Human primary CD8+ T cells were purchased from STEMCELL Technologies Inc ... -
Cytoskeleton
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... chicken gizzard smooth muscle actin (Cytoskeleton, AS99), beta actin (NM_001101 ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in eLife doi: 10.7554/elife.37921Quote: Human skeletal muscle total RNA was from Clontech (USA) and cDNA was synthesized as described above ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: Skeletal muscle-derived primary myoblasts were isolated from wild-type mice using the Satellite Cell Isolation Kit MACS protocol (Miltenyi Biotec). Briefly ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: Human primary lung fibroblast IMR-90 cells (American Type Culture Collection, ATCC) were initially cultured in Eagle’s minimum essential medium (EMEM ... -
BioLegend
No products found because this supplier's products are not listed.Cited in CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Following antibodies were purchased and used for aortic cell staining: CD45 (30F-11, BD Bioscience and Biolegend), CD31 (MEC13.3 ... -
Progen Biotechnik GmbH
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Nature Communications doi: 10.1038/s41467-018-03973-8Quote: ... Smooth muscle cells and pericytes were labelled using a biotinylated anti-α-smooth muscle actin (α-SMA) primary antibody (Progen, Heidelberg, Germany) and streptavidin-Cy-3 (Dianova ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in PLOS Biology doi: 10.1371/journal.pbio.3000632Quote: LDH from rabbit muscle (Roche) was diluted to 5 µg/mL in 5-fold diluted boiled supernatants (for Figure 1E) ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2018, published in PLOS Computational Biology doi: 10.1371/journal.pcbi.1006238Quote: Mouse cardiac muscle HL-1 cells purchased from Merck Millipore were grown on fibronectin(25µg/ml)/gelatin(1mg/ml ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Genetics 2020Quote: ... human stem cell factor 50ng/ml (PeproTech 300-07), erythropoietin 50ng/ml (Peprotech 100-64) ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019Quote: Smooth muscle cells (A10 and MOVAS) were fixed in methanol-free 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, cat. no. 15710) for 10 mins diluted in PBS++ (Thermo Fisher Scientific ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018Quote: ... TRPP1fl/fl mice were crossed with tamoxifen-inducible smooth muscle-specific Cre mice (SMMHC-CreERT2, Jackson Labs, ref. 36) to produce PKD2fl/fl:smCre+ mice ... -
Proteintech
No products found because this supplier's products are not listed.bioRxiv - Pathology 2020Quote: ... and Sirius red were routinely performed and α-smooth muscle actin (α-SMA) was detected by immunohistochemical staining using anti-α-SMA (Proteintech, United States) as described previously[47] ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Cell Reports doi: 10.1016/j.celrep.2020.01.097Quote: ... was performed by incubating dissected and minced TA muscles in 1 ml satellite cell medium containing 3 μM SNAP Cell SiR 647 (New England Biolabs, S9102S) for 30 min at 37°C with gentle agitation ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Physiology 2019Quote: ... 5 mm of the mid-belly of lateral gastrocnemius muscle was cut into 10-μm thick frozen muscle cross-sections at −20°C with a cryostat (Leica, Wetzlar, CM1850, Germany). Because of the uneven thickness of the diaphragm ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018, published in Arthritis & Rheumatology doi: 10.1002/art.40931Quote: ... Lysed whole blood cells were incubated with Human Seroblock (BIO-RAD) in cell staining buffer (BioLegend ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... The H2B-mRFP expression construct for human cells was obtained from Addgene (#26001), transfected to 293T cells to produce viruses and infect HeLa Cyclin B1-Venus expressing cells to generate a stable cell line ... -
GE Life Sciences
No products found because this supplier's products are not listed.Cited in NK cells prevent the formation of teratomas derived from human induced pluripotent stem cellsbioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ... -
Cell Biologics
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Primary human pulmonary microvascular endothelial cells (Cell Biologics, USA) were expanded in 75 cm2 tissue culture flasks using human endothelial cell growth medium (Cell Biologics ... -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2017, published in Scientific Reports doi: 10.1038/s41598-018-28846-4Quote: ... Primary cultures of skeletal muscle cells were prepared from newborn (1-2 days-old) mice (CD1, Charles River) as reported by Massimino et al. ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... and the aortic arch was visualized using a binocular operating stereoscope (Olympus). TAC interventions used a spacer-defined (26-gauge ... -
Nikon
No products found because this supplier's products are not listed.Cited in MicroRNA-27a mitigates hyperlipidemia and atherosclerosis by modulating cholesterol homeostasisbioRxiv - Molecular Biology 2018, published in Molecular and Cellular Biology doi: 10.1128/MCB.00470-19Quote: ... Photographs of the exposed aortic arches were taken by a dissecting microscope (Nikon, C-FLED2). For microscopic examination ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020, published in Journal of Proteome Research doi: 10.1021/acs.jproteome.0c00521Quote: ... as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch, 1:2000) or goat anti-rabbit IgG-HRP (Abcam # ab6721 ... -
Novus Biologicals
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020, published in Science Signaling doi: 10.1126/scisignal.abb9820Quote: ... Anti-ELP4 (rabbit anti-human primary, CAT# NBP2-16322) antibody was purchased from Novus Biologicals. Monoclonal anti-β-actin (mouse anti-human primary ... -
Invivogen
No products found because this supplier's products are not listed.Cited in The fungal ligand chitin directly binds and signals inflammation dependent on oligomer size and TLR2bioRxiv - Immunology 2018Quote: The human monocytic THP-1 cell line (Invivogen) and two THP-1-based TLR2 Cas9-CRISPR-edited clones or their parental cell lines (a gift from V ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... Muscles were then washed in 0.1 M PB and mounted in Vectashield (Vector Laboratories). Z-stacks were recorded using a confocal microscope (FV1000 ... -
MatTek
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2017Quote: Human osteosarcoma U2OS cells were cultured on 35 mm glass bottom dishes (MatTek) at 37°C in Dulbecco-modified Eagle’s Minimum Essential Medium (DMEM ... -
Greiner
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in British Journal of Cancer doi: 10.1038/s41416-020-0923-6Quote: ... 5×103 human SCLC cells were seeded in a 96-well cell culture plate (Cat no.: 655180; Greiner Bio-One). After allowing cells to adhere during a 4h incubation at 37°C ... -
ibidi
No products found because this supplier's products are not listed.bioRxiv - Physiology 2020, published in Antioxidants doi: 10.3390/antiox9080710Quote: Skeletal muscle myoblasts were seeded on a 35-mm glass-bottom μ-Dish (ibidi®, Martinsried, Germany), treated as previously described ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Genomics 2018Quote: We compiled 64 available DNAm datasets of untreated primary cells with reliable information on passage numbers (all Illumina 450k Methylation BeadChip ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Acta Histochemica doi: 10.1016/j.acthis.2018.08.004Quote: ... the cell samples were incubated with fluorescein isothiocyanate (FITC) -conjugated mouse anti-human CD31 (Beckman Coulter Inc., USA). Subsequently ... -
Cell Systems
ACBRI 716 cells were isolated by centrifugal counterflow elutriation from dissociated normal...Cat# ACBRI 716, Frozen Vial with 1E6 cells, USD $735.0/1ea AskbioRxiv - Pharmacology and Toxicology 2018, published in Journal of Pharmacology and Experimental Therapeutics doi: 10.1124/jpet.118.248088Quote: Primary human retinal microvascular endothelial cells (HRECs) were obtained from Cell Systems, Inc ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018, published in Oncotarget doi: 10.18632/oncotarget.26966Quote: HT29-Luc2 Bioware® Ultra human colorectal cancer cells were purchased from (PerkinElmer/cat: 124353) and were maintained at 37 °C / 5% CO2 in McCoy’s 5A medium (Gibco ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in eLife doi: 10.7554/eLife.38461Quote: Human LRRC8A (Origene #RC208632) was cloned with a C-terminal FLAG-tag (DYKDDDDK ...