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No products found
because this supplier's products are not listed.
Quote:
... Primary human aortic endothelial cells (HAECs) were purchased from Lonza and grown in EMB-2 media supplemented with EGM-2 SingleQuot Kits (Lonza) ...
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No products found
because this supplier's products are not listed.
Eva Jover, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Human aortic endothelial cells (AorECs) (C-12271, Promocell, UK) were cultured in complete EGM2 following manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Mabruka Alfaidi, et al.,
bioRxiv - Pathology 2020
Quote:
Human aortic endothelial cells (HAECs, CELL Applications) were maintained in MCDB131 containing 10% (v/v ...
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No products found
because this supplier's products are not listed.
Barbara Kutryb-Zajac, et al.,
bioRxiv - Biochemistry 2018
Quote:
... Human primary aortic valve endothelial (hVEC) and interstitial (hVIC) cells intended to IF were seeded on 96-well optical-bottom plate (Nunc ThermoFisher, USA) at a density 1 × 104 cells/ well in a total volume of 200 uL cell culture medium ...
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No products found
because this supplier's products are not listed.
Quote:
... and endothelial cell growth supplement (Sigma Aldrich). Human pericytes (PCs ...
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No products found
because this supplier's products are not listed.
Elina Shrestha, et al.,
bioRxiv - Genetics 2018
Quote:
Apoptosis was analyzed in the aortic roots by staining the plaques with anti-cleaved caspase 3 (Cell signaling) and counter stained with hematoxylin ...
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No products found
because this supplier's products are not listed.
Longlong Si, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary human pulmonary microvascular endothelial cells (Cell Biologics, USA) were expanded in 75 cm2 tissue culture flasks using human endothelial cell growth medium (Cell Biologics ...
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No products found
because this supplier's products are not listed.
Quote:
... Primary human cerebromicrovascular endothelial cells (HBMEC) were purchased from Sciencell Research Laboratories (San Diego ...
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No products found
because this supplier's products are not listed.
Quote:
... Immunostaining of endothelial cells was performed with a rat anti-mouse CD31 primary antibody (BD Biosciences, Heidelberg, Germany), followed by a donkey anti-rat Cy-3-labeled secondary antibody (Dianova ...
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No products found
because this supplier's products are not listed.
Elina Shrestha, et al.,
bioRxiv - Genetics 2018
Quote:
Cell proliferation in the plaques were analyzed in aortic roots by staining the plaques for Ki67 (abcam). Nuclei positively stained for the antibody and hematoxylin were considered proliferating cells ...
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No products found
because this supplier's products are not listed.
Wanling Xuan, Mahmood Khan, Muhammad Ashraf,
bioRxiv - Cell Biology 2020
Quote:
Human aortic endothelia cells (HAEC, 1×105 cells/well) were seeded on Matrigel (Corning) in a 24-well plate and treated with or without 1 μg EV from different groups of Givi-MPC ...
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No products found
because this supplier's products are not listed.
Hong-hong Zou, et al.,
bioRxiv - Biochemistry 2018
Quote:
Primary endothelial cells were obtained from the following procedures: pronase (Roche, Switzerland) followed by collagenase (Roche ...
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No products found
because this supplier's products are not listed.
Quote:
... and endothelial cells with anti-CD31 (PCAM-1) antibody (1:500; Santa Cruz). A secondary Alexa 647 antibody (1:600 ...
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No products found
because this supplier's products are not listed.
Hafsa Munir, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... endothelial cells (CD31) and epithelial cells (EpCAM clone G8.8; all from Biolegend).
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No products found
because this supplier's products are not listed.
Akira Ito, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Total RNA was extracted from the isolated endothelial cells using shredder (Qiagen) and RNeasy Mini Kit (Qiagen ...
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CSC 2B2 cells were isolated by centrifugal counterflow elutriation from 8 individual donor veal...
Cat# CSC 2B2 V,
Frozen Vial with 2E6 cells/mL, USD $500.0/1ea
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Quote:
Primary human retinal microvascular endothelial cells (HRECs) were obtained from Cell Systems, Inc ...
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No products found
because this supplier's products are not listed.
Bowen Wang, et al.,
bioRxiv - Pathology 2019
Quote:
... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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No products found
because this supplier's products are not listed.
Vincenza Caolo, et al.,
bioRxiv - Cell Biology 2019
Quote:
Endothelial cells were seeded on glass slides (MENSJ5800AMNZ, VWR, Radnor, PA) coated with Fibronectin (F0895 ...
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No products found
because this supplier's products are not listed.
Jason M. Booe, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... and primary human umbilical vein endothelial cells (HUVECs) (PCS-100-010) were from American Type Culture Collection (Manassas, VA, USA). Dulbecco’s modified eagle media (DMEM ...
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No products found
because this supplier's products are not listed.
Quote:
... Membranes were blocked with 5% bovine serum albumin and incubated with the appropriate primary and HRP-conjugated secondary antibodies (GE Healthcare). Western Lightning ECL Pro (PerkinElmer ...
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No products found
because this supplier's products are not listed.
Quote:
... The flow cell was then treated with bovine serum albumin (BSA, New England Biolabs) (100 mg/ml ...
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No products found
because this supplier's products are not listed.
Quote:
... endothelial (Ibidi) and adipose tissue (LB2 ...
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No products found
because this supplier's products are not listed.
Peng Chen, et al.,
bioRxiv - Systems Biology 2019
Quote:
... Endothelial cells were isolated using CD31-conjugated microbeads (Miltenyi Biotec). Initially ...
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No products found
because this supplier's products are not listed.
Quote:
... Endothelial cells were isolated by administration of 0.1% collagenase D solution from Clostridium histolyticum (Merck KGaA) into the vein for 20 minutes at 37°C and subsequently cultured in endothelial cell growth medium purchased from PromoCell supplemented with 1% penicillin/streptomycin and used up to passage 4 for all indicated experiments.
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No products found
because this supplier's products are not listed.
Christina Kim, et al.,
bioRxiv - Biophysics 2019
Quote:
... Endothelial cell phenotype was confirmed by the typical cobblestone appearance and von Willebrand Factor expression (A0082 Dako-Agilent Santa Clara CA) (Supplemental Figure 1) ...
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No products found
because this supplier's products are not listed.
Marley J. Dewey, et al.,
bioRxiv - Bioengineering 2020
Quote:
Expression levels of cell secreted Osteoprotegerin and Vascular Endothelial Growth Factor (VEGF) was examined via ELISA (R&D Systems, Minnesota, USA). Briefly ...
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No products found
because this supplier's products are not listed.
Holly Holliday, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Primary cells were resuspended in Epicult-B (Stemcell Technologies) media containing 2% Matrigel and 12,000 cells were seeded into each chamber in a total volume of 400 μL ...
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No products found
because this supplier's products are not listed.
Quote:
... Membranes were incubated at 4°C in primary antibodies diluted 1:1000 in 5% bovine serum albumin: anti-GFP (ClonTech Living Colours #ab632375), anti-HSP60 (Department of Biology ...
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No products found
because this supplier's products are not listed.
Shuping Zhang, et al.,
bioRxiv - Cell Biology 2018
Quote:
... Cells were sorted into tubes with 20% fetal bovine serum (FBS, Atlanta Biologicals, Norcross, GA) to help preserving cell integrity.
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No products found
because this supplier's products are not listed.
Jessie Wang, et al.,
bioRxiv - Physiology 2020
Quote:
... The following antibodies were selected and tested on half of each cornea: anti-CD31 mouse monoclonal IgG1 targeting vascular endothelial cells (BioRad, Kidlington, Oxford, England), anti-Neutrophil rabbit polyclonal IgG targeting neutrophils (LifeSpan BioSciences ...
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No products found
because this supplier's products are not listed.
Chinnaswamy Tiruppathi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... endothelial cells grown on High Tolerance Coverslips (pcs-170-1818, MatTek) were washed ...
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No products found
because this supplier's products are not listed.
Maud de Dieuleveult, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells were incubated with primary anti-USP9X antibody (Bethyl Laboratories ...
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No products found
because this supplier's products are not listed.
Quote:
Four representative sections from each aortic and heart graft were stained with hematoxylin and eosin (H&E; Leica Biosystems, Buffalo Grove, IL) or Movat’s pentachrome (Newcomer Supply ...
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No products found
because this supplier's products are not listed.
Lucie Peskova, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 2% Foetal Bovine Serum in PBS) and incubated for 1 hour with the primary antibodies SOX2 (NB110-37235, Novus Biological, 1:400) or Oct-3/4 (sc-5279 ...
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No products found
because this supplier's products are not listed.
Vinod Kumar, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... Cells were incubated with the following primary antibodies: m-APOL1 (1:100, Proteintech), rb-PAX2 (1:200 ...
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No products found
because this supplier's products are not listed.
Quote:
... cells were hormone-deprived in 10% charcoal-stripped dextran-treated fetal bovine serum (Omega Scientific) media for 2 days and then treated with indicated concentration of DHT for 24 h ...
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No products found
because this supplier's products are not listed.
Quote:
... or goat anti-mouse IgM (for primary B cells; Jackson ImmunoResearch, #115-005-020) as described (Lin et al. ...
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No products found
because this supplier's products are not listed.
Quote:
... containing 10 % foetal bovine serum (FBS, Greiner Bio-one ...
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No products found
because this supplier's products are not listed.
Christian E. Badr, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... All three cell lines were grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (Gemini Bio-products), 100U penicillin ...
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No products found
because this supplier's products are not listed.
Filip Berisha, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and the aortic arch was visualized using a binocular operating stereoscope (Olympus). TAC interventions used a spacer-defined (26-gauge ...
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No products found
because this supplier's products are not listed.
Abrar A. Khan, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... Photographs of the exposed aortic arches were taken by a dissecting microscope (Nikon, C-FLED2). For microscopic examination ...
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No products found
because this supplier's products are not listed.
Quote:
... Following blocking in 5% milk or bovine serum albumin the blots were incubated with primary antibodies and analyzed by HRP-conjugated secondary antibodies (Jackson Laboratories). The following primary antibodies were used in the study ...
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No products found
because this supplier's products are not listed.
Quote:
Primary cultured cells (300 µL) were seeded in the inserts of 24 well cell migration plate (Cell Biolabs Inc ...
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No products found
because this supplier's products are not listed.
Quote:
Primary antibodies used in Drosophila cells were: anti-γH2Av (1:1000, Rockland, 600-401-914); anti-GFP (1:1000 Aves Lab ...
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No products found
because this supplier's products are not listed.
Quote:
... Cells were then fixed and stained with biotinylated dolichos lectin (primary; 1:400; Vector Laboratories) and Streptavidin Alexa350 (secondary ...
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No products found
because this supplier's products are not listed.
Quote:
... Cells were sorted into staining buffer (BSS, 2% fetal bovine serum, plus sodium azide) by a MoFloXDP (Beckman Coulter Life Sciences or Synergy SY3200 (iCyt ...
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No products found
because this supplier's products are not listed.
Quote:
... Patients with a diagnosis of FECD and scheduled for endothelial keratoplasty between the dates of 2/12/2013 and 10/27/2014 (for Illumina Infinium HumanMethylation450 BeadChip analysis ...
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No products found
because this supplier's products are not listed.
Quote:
... whereas HAP1 cells were cultured using Isocovés modified DMEM media (IMDM) supplemented with 20 % fetal bovine serum (PAN Biotech), 2 mM glutaMAX (Gibco ...
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No products found
because this supplier's products are not listed.
Quote:
Primary culture-Cells were fixed for 10 minutes at room temperature in 4% (w/v) paraformaldehyde (PFA, EMS) in PBS (Sigma) ...
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No products found
because this supplier's products are not listed.
Quote:
... Primary antibodies (anti-H3K27me2me3, Active Motif 39535 ...