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No products found
because this supplier's products are not listed.
Quote:
4-15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad)
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No products found
because this supplier's products are not listed.
Valeria Lulla, Andrew E. Firth,
bioRxiv - Microbiology 2019
Quote:
... and precast Novex™ 10–20% tricine protein gels (Thermo Fisher) to resolve XPs and enterovirus 2B ...
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No products found
because this supplier's products are not listed.
Quote:
Cell lysates (30 µg) were separated on 4–15% precast polyacrylamide gels (Mini-PROTEAN® TGX™ Precast Protein Gels, Bio-Rad) and were transferred to nitrocellulose membranes ...
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No products found
because this supplier's products are not listed.
Maruša Bizjak, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... were loaded on a 4–20% polyacrylamide gel (TruPAGE™ Precast Gels, Sigma) and separated using electrophoresis (Mini-protean tetra cell system ...
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No products found
because this supplier's products are not listed.
Quote:
... After loading 35 µg of protein onto 4-20% (wt/vol) precast gels (Lonza), proteins were transferred to a PVDF membrane followed by blocking with 5% milk for 1 hr at RT ...
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No products found
because this supplier's products are not listed.
Marc Bayliss, et al.,
bioRxiv - Immunology 2020
Quote:
... the samples were loaded onto a RunBlue precast gel (Expedeon) and run in RunBlue running buffer (Expedeon ...
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No products found
because this supplier's products are not listed.
Quote:
... and the supernatant was subjected to a GST tag protein purification gel and His tag protein purification gel (GE Healthcare) for affinity purification ...
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No products found
because this supplier's products are not listed.
Charles P. Moehs, et al.,
bioRxiv - Plant Biology 2018
Quote:
... In-gel digestion of proteins with chymotrypsin (Promega) for 16 hr at 37° C followed by Trypsin (Promega ...
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No products found
because this supplier's products are not listed.
Kun Xu, et al.,
bioRxiv - Plant Biology 2018
Quote:
... or an 8% precast SurePageTM Gel (GenScript, China) for GmGRF5-1/GmFTL3.F4 ...
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No products found
because this supplier's products are not listed.
Bruno Pok Man Ngou, et al.,
bioRxiv - Plant Biology 2020
Quote:
... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Quote:
... gel purified (MinElute Gel Extraction Kit, Qiagen) and loaded on an Illumina MiniSeq instrument in mid-output mode ...
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No products found
because this supplier's products are not listed.
Quote:
... The proteins were separated by gel-electrophoresis (10% polyacrylamide gel) and transferred onto a PVDF membrane (Roche) using a semi-dry blotting system (BioRad) ...
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No products found
because this supplier's products are not listed.
Quote:
... Gel filtered proteins were labeled with either SNAP-Cell 505 (NEB, catalog S9103S) or SNAP-Cell TMR (NEB ...
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No products found
because this supplier's products are not listed.
Chatterjee Amit, et al.,
bioRxiv - Cell Biology 2019
Quote:
... in Bio-Rad Gel Documentation system (Protein Simple).
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No products found
because this supplier's products are not listed.
Ryan R. Richardson, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... gel extracted with Zymoclean Gel DNA Recovery kit (Zymo Research), and submitted for Sanger sequencing (Genewiz) ...
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No products found
because this supplier's products are not listed.
Roshni Srivastava, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... and protein A/G agarose gel were purchased from Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Aakanksha Wany, et al.,
bioRxiv - Plant Biology 2018
Quote:
... Protein from gel was transferred to nitrocellulose blotting membrane (Pall Corporation, Florida) in a wet transblot system (BIORAD ...
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No products found
because this supplier's products are not listed.
Jessie Low-Gan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Each elution fraction was resolved on an SDS-PAGE gel stained with InstantBlue Coomassie Protein Stain (Abcam), and fractions containing a single RBD band were pooled ...
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No products found
because this supplier's products are not listed.
Robin Hoeven, et al.,
bioRxiv - Bioengineering 2019
Quote:
... PCR products were analysed by agarose gel electrophoresis and gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Constructs were transformed into E ...
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No products found
because this supplier's products are not listed.
Emily Bowler, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... on a 0.9% agarose gel using GelRed® Nucleic Acid Gel Stain (Biotium) and imaged using a G:BOX Ef imaging system (Syngene ...
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No products found
because this supplier's products are not listed.
Quote:
... and then applied to 8% precast gel (PG00810-N, Solarbio, China) to analyze the complex formation between Tf and thrombin/FXIIa in running buffer (0.05 M Trizma ...
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No products found
because this supplier's products are not listed.
Paul R.F. Cordero, et al.,
bioRxiv - Microbiology 2019
Quote:
... gels were incubated in AcquaStain Protein Gel Stain (Bulldog Bio) at 4°C for 3 hr ...
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No products found
because this supplier's products are not listed.
Alyson M. Hoffman, Christopher V. Nicchitta,
bioRxiv - Cell Biology 2018
Quote:
Gel bands of interest were isolated using a sterile scalpel transferred to protein LoBind tubes (Eppendorf) and minced ...
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No products found
because this supplier's products are not listed.
Chen Cheng, et al.,
bioRxiv - Molecular Biology 2017
Quote:
Analysis and quantification of protein gels and WB were performed using the Odyssey Infrared Imaging System (LI-COR Biosciences). Histone protein concentration was initially quantified with the BCA assay (Thermo Scientific #23225 ...
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No products found
because this supplier's products are not listed.
Hong-Yun Tham, et al.,
bioRxiv - Molecular Biology 2019
Quote:
The rEDIII protein band was excised from polyacrylamide gel and the sample was prepared for de novo protein sequencing using in-gel digestion according to manufacturer’s protocol (Agilent Technologies, Inc., 2015). Briefly ...
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No products found
because this supplier's products are not listed.
Joshua M. Weiss, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and then gel purified using NucleoSpin® Gel & PCR Clean-up Midi (Takara # 740986.20). for deep sequencing using the CRISPR-seq platform 88 ...
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No products found
because this supplier's products are not listed.
Quote:
... DNA was extracted from the gel slices using the peqGOLD Gel Extraction Kit (VWR). Genotyping was done using a modified ast-PCR protocol for R21X (Supplemental methods ...
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No products found
Quote:
Bio-Rad protein electrophoresis (Mini-PROTEAN® Tetra Cell Systems and TGX™ precast gels) and transfer system (Trans-Blot® Turbo Transfer System ...
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No products found
because this supplier's products are not listed.
Quote:
We used SYPRO Ruby Protein Gel stain (Lonza, Rockland, ME) to visualize the in vitro pulldowns in Figure 4B ...
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No products found
because this supplier's products are not listed.
Tao Fan, et al.,
bioRxiv - Cell Biology 2018
Quote:
... Fifty micrograms of protein was subjected to SDS-polyacrylamide gel electrophoresis (12%PAGE; Amresco) and transferred to a polyvinylidene fluoride membrane (Millipore ...
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No products found
because this supplier's products are not listed.
Bénédicte Desvoyes, et al.,
bioRxiv - Plant Biology 2019
Quote:
... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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No products found
because this supplier's products are not listed.
Quote:
... 10 μg of total protein was loaded to the gel and anti-DET1 polyclonal antibody (Agrisera Antibodies AS153082) was used at a 1:1000 dilution ...
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No products found
because this supplier's products are not listed.
Quote:
... Equal OD units of cells were loaded onto acrylamide gels and proteins were separated using a Bis-Tris-based gel system [123] with 1 X MOPS-SDS running buffer (National Diagnostics) before semi-dry transfer onto a PVDF membrane (Thermo Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Quote:
... Proteins were prepared by adding 6X reducing Laemmli buffer and boiling for 5 min and a total of 5 μg of total protein extracts or 0.5 μg of purified histones were separated on denaturing 10% or 15% SDS-PAGE gels based on the target size and proteins were transferred onto PVDF transfer membranes (0.45 μm, Perkin Elmer, Waltham, MA). Membranes were probed with the following primary antibodies ...
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No products found
because this supplier's products are not listed.
Quote:
... The libraries were gel-purified using the E.Z.N.A Gel Extraction Kit (Omega Bio-Tek). Sequencing was performed using a HiSeq 4000 ...
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No products found
because this supplier's products are not listed.
Edward D. Kerr, et al.,
bioRxiv - Biochemistry 2020
Quote:
Proteins and peptides were identified with Protein Pilot 5.1 (SCIEX) as previously described1,16 ...
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No products found
because this supplier's products are not listed.
Quote:
... coverslipped with Fluoro-Gel (Electron Microscopy Sciences), and sealed.
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No products found
because this supplier's products are not listed.
Quote:
... proteins were extracted using the ISOLATE II RNA/DNA/Protein Kit (BIOLINE). Protein concentration was determined using the MicroBCA Protein Assay Kit (Pierce-Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Dharendra Thapa, et al.,
bioRxiv - Physiology 2019
Quote:
... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein concentration was measured using Precision Red protein assay reagent (Cytoskeleton, Inc.). ∼40 μg lysates were separated by SDS-PAGE and transferred onto the Immobilon P PVDF membrane (EMD Millipore) ...
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No products found
because this supplier's products are not listed.
Quote:
... All fragments obtained by polymerase chain reaction were gel-purified using a DNA Gel Extraction kit (Axygen, Corning, NY, USA) according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
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No products found
because this supplier's products are not listed.
Quote:
... We therefore identified the brain development protein-protein interaction network (BD-PPI network) for the 22q11.2 locus (i.e. ...
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No products found
because this supplier's products are not listed.
Quote:
... MS2 coat protein (Addgene #27121) was fused with superfolder GFP (Kersey et al. ...
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No products found
because this supplier's products are not listed.
Quote:
... recombinant ephrinA4-Fc protein (R&D Systems) was added at the start of the coculture to a final concentration of 2µg/ml.
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No products found
because this supplier's products are not listed.
C. Cassarini, Y. Zhang, P. N. Lens,
bioRxiv - Microbiology 2018
Quote:
... After digestion purification was done by gel extraction (Gel extraction Kit, Omega Biotek, USA). The copy number was calculated from the total mass and the nucleic acid concentration ...
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No products found
because this supplier's products are not listed.
Quote:
For immunoprecipitation 0.8 μl anti V5 antibody was added to 500 μl cell lysate and incubated over-night on a rotating wheel at 4°C and the protein complex was precipitated by using 50 μl of Protein A and Protein G Plus-Agarose beads (Calbiochem) in EBC buffer ...
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No products found
because this supplier's products are not listed.
Quote:
... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ...
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No products found
because this supplier's products are not listed.
Quote:
Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Quote:
... except that HER2-His protein (Sino Biological) was used as the capture reagent for the total antibody and total ADC measurements.