-
No products found
because this supplier's products are not listed.
Benjamin Kirkup, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Tissue culture plastic was coated with 0.1% porcine gelatin (Sigma) in water for 1 hour at 37°C prior to culture.
-
No products found
because this supplier's products are not listed.
Ojas Deshpande, et al.,
bioRxiv - Cell Biology 2020
Quote:
Purified porcine Tubulin (Cytoskeleton) was labeled with Alexa-647 (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Quote:
... Mouse skin tissue lysate was prepared by homogenization in T-PER Tissue Protein Extraction Buffer (Thermo Scientific, 78510) with protease inhibitors (Roche ...
-
No products found
because this supplier's products are not listed.
Quote:
... modified porcine trypsin (Promega) was dissolved in 50mM acetic acid and diluted with 25mM ammonium bicarbonate to give a final trypsin concentration of 0.02g/L ...
-
No products found
because this supplier's products are not listed.
Feini Qu, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... Frozen tissues were crushed with a pestle and the tissue lysates homogenized (QIAshredder, QIAGEN). Total RNA was isolated (RNeasy Mini Kit ...
-
No products found
because this supplier's products are not listed.
Quote:
Cell and tissue lysates were denatured in Laemmli sample buffer (Bio-Rad, Hercules, CA) and resolved by Tris-glycine SDS-PAGE (4-20% polyacrylamide ...
-
No products found
because this supplier's products are not listed.
Eliza Mari Kwesi-Maliepaard, et al.,
bioRxiv - Immunology 2019
Quote:
... Porcine (BHI) medium (BD) to an absorbance at 600 nm of OD 0.9 ...
-
No products found
because this supplier's products are not listed.
Quote:
Tissue and cell lysates were prepared using Bioruptor (Diagenode) in RIPA buffer (10 mM Tris-HCl ...
-
No products found
because this supplier's products are not listed.
Jamie L. Grit, et al.,
bioRxiv - Genomics 2020
Quote:
... Protein lysates were prepared by manually homogenizing frozen tissue in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Proteins were separated on a 4-20% TGX SDS-PAGE gel (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Quote:
... Whole brain lysate was prepared accordingly after grinding the tissue on ice with a pellet mixer (VWR, Radnor, PA, USA) in ice-cold lysis buffer.
-
No products found
because this supplier's products are not listed.
Quote:
... and following the protocol “Preparation of lysate from tissues” from Abcam with modifications ...
-
No products found
because this supplier's products are not listed.
Tetiana Brodiazhenko, et al.,
bioRxiv - Biochemistry 2018
Quote:
... the lysates were treated with micrococcal nuclease (NEB) (24 units/μl ...
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No products found
because this supplier's products are not listed.
Meghan M. Capeling, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... Tissue was processed into paraffin using an automated tissue processor (Leica ASP300). Paraffin blocks were sectioned 7 uM thick ...
-
No products found
because this supplier's products are not listed.
Genevieve C. K. Wilson, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Protein lysates were prepared by homogenizing hindlimb muscle tissue in myosin extraction buffer (0.3M NaCl ...
-
No products found
because this supplier's products are not listed.
Quote:
Porcine and bovine brain tubulin was labelled with Cy5-or Alexa-488 NHS ester (GE Healthcare) to 54% labelling using previously described methods (Gell et al. ...
-
No products found
because this supplier's products are not listed.
Joseph Amick, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Lysates were incubated with GFP-Trap resin (ChromoTek) for 2 h at 4°C ...
-
No products found
because this supplier's products are not listed.
Aran Singanayagam, et al.,
bioRxiv - Immunology 2019
Quote:
... mice were treated intranasally under light isoflurane anaesthesia with 1.2 units of porcine pancreatic elastase (Merck, UK) to induce COPD-like changes ...
-
No products found
because this supplier's products are not listed.
Quote:
... or tissue culture treated (Corning, 430641U) flasks ...
-
No products found
because this supplier's products are not listed.
Shigeki Hirabayashi, et al.,
bioRxiv - Genomics 2019
Quote:
... The tissue lysate was then filtered through gentleMACS SmartStrainers (100 μm) (Miltenyi Biotec) and 200 μL of the lysate was sampled and mixed with 700 μL of QIAzol for total RNA extraction ...
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No products found
because this supplier's products are not listed.
Quote:
... Clarified lysate was added to Talon resin (Clontech) and incubated 1 hr at 4° C ...
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No products found
Zeenat B. Noordally, et al.,
bioRxiv - Plant Biology 2018
Quote:
... Porcine trypsin TPCK treated was from Worthington (Lorne Laboratories). All HPLC-MS connector were from Upchurch Scientific or Valco (Hichrom and RESTEK) ...
-
No products found
because this supplier's products are not listed.
Jiuwei Lu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Cell lysate was centrifuged with a JA25.5 rotor (Beckman Coulter) at 39,000 g for 40min at 4 °C ...
-
No products found
because this supplier's products are not listed.
Paul R. Marshall, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Lysate from cells or tissue was fixed in 1% formaldehyde and cross-linked cell lysates were sheared by Covaris in 1% SDS lysis buffer to generate chromatin fragments with an average length of 300bp ...
-
No products found
because this supplier's products are not listed.
Quote:
RNA for analysis of gene expression with qRT-PCR from cell lysates and frozen tumor tissue was extracted using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany). Subsequent reverse transcription was performed with the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Quote:
... we lysed tissue samples with appropriate buffers for animal tissue and purified DNA from tissue lysates using the E.Z.N.A Tissue DNA extraction kit (Omega Bio-tek). To minimize enzymatic inhibitor that co-purify with genomic DNA from P ...
-
No products found
because this supplier's products are not listed.
Vahid Kheirollahi, et al.,
bioRxiv - Cell Biology 2018
Quote:
Total protein lysates were prepared using RIPA buffer (Santa Cruz) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Quote:
... cell lysates were harvested using RIPA buffer (Cell Signaling Technologies) with protease inhibitors (cOmplete ...
-
No products found
because this supplier's products are not listed.
Quote:
... Lysate was transferred to EasyTabC glass fibre filters (Perkin Elmer) using Filtermate cell harvester and scintillation counts were measured using Microscint-O in Topcount NXT Beta Counter (Packard/ Perkin Elmer).
-
No products found
because this supplier's products are not listed.
Wanlin Yang, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Tissues were embedded in Tissue-Tek O.C.T (Electron Microscopy Sciences, Hatfield, PA) and sectioned into 45 μm cryo-sections using a Leica Cryostat (Model# CM1900) ...
-
No products found
because this supplier's products are not listed.
Mark A. DeWitt, et al.,
bioRxiv - Cell Biology 2016
Quote:
... Cell lysates were characterized by HPLC (Infinity 1260, Agilent) using a weak cation-exchange column (PolyCAT A™ ...
-
No products found
because this supplier's products are not listed.
Isabel Mogollón, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Tissues were mounted with Vectashield (Vector Laboratories) and imaged with either Leica Biosystems TCS SP5 microscope and HC PL APO 10×/0.4 (air) ...
-
No products found
because this supplier's products are not listed.
Quote:
... Cells were lysed and the lysates transferred to all-white 96-well flat solid bottom plates suitable for tissue culture and luminescence measurements (Greiner Bio-One, Kremsmünster, Austria). Sample preparation from cell lysates and luciferase luminescence measurement were performed according to the manufacturer’s protocol for the Dual-Luciferase Reporter Assay System (Promega) ...
-
No products found
because this supplier's products are not listed.
Roman Spektor, et al.,
bioRxiv - Genomics 2019
Quote:
... Tissue was homogenized using a Dounce Tissue Grinder set (Wheaton #357542) with 10 strokes using a loose pestle and filtered through a 100 µm nylon mesh (VWR #10199-658 ...
-
No products found
because this supplier's products are not listed.
Catalina Sierra-Ramos, et al.,
bioRxiv - Physiology 2020
Quote:
Total RNA was obtained from tissues disrupted with a Tissue Tearor (BioSpec Products) or from cultured cells using a commercial kit (Total RNA Spin Plus ...
-
No products found
because this supplier's products are not listed.
Yeo Min Yoon, et al.,
bioRxiv - Cell Biology 2018
Quote:
... and HGF in hindlimb ischemia–associated CKD tissue lysates were determined with commercially available ELISA kits (R&D Systems, Minneapolis, MN, USA). On postoperative day 3 ...
-
No products found
because this supplier's products are not listed.
Yang Su, et al.,
bioRxiv - Cancer Biology 2020
Quote:
FFPE tissue arrays (US Biomax) were deparaffinized in xylene overnight and rehydrated by sequential exposure to 100% ...
-
No products found
because this supplier's products are not listed.
Quote:
... Genomic DNA from tissues was isolated using the ZR Genomic DNA-Tissue Kits (Zymo Research) according to the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Quote:
... Cellular lysates were prepared by a FastPrep homogeniser (MP Biomedicals) (three 20 seconds pulses at speed 6.0 mp/sec with chilling on ice for 1 minutes between the cycles ...
-
No products found
because this supplier's products are not listed.
Sierra Fox, et al.,
bioRxiv - Genetics 2019
Quote:
... Cell lysate was resuspended in transposition reaction mix (Illumina) and incubated at 37°C for 30 min ...
-
No products found
because this supplier's products are not listed.
Ingrid Gomez, et al.,
bioRxiv - Cell Biology 2018
Quote:
... and for endotoxin contamination using Limulus Amebocyte lysate assay (Lonza, UK) and found to contain neither ...
-
No products found
because this supplier's products are not listed.
Quote:
... Labeled tissue was imaged on a Fluoview FV1000 confocal microscope (Olympus). In each experiment ...
-
No products found
because this supplier's products are not listed.
Quote:
Whole cell lysate or tissue lysate was extracted using RIPA lysis buffer (Boston Bioproducts) supplemented with complete protease inhibitor cocktail (Roche) ...
-
No products found
because this supplier's products are not listed.
Nilsa La Cunza, et al.,
bioRxiv - Neuroscience 2020
Quote:
... E3 or E4 expressing primary porcine RPE cell cultures were imaged using the Andor Revolution XD with 100X/1.49 NA Apo TIRF objective (Nikon, Melville, NY) for ∼50 frames at 37°C ...
-
No products found
because this supplier's products are not listed.
Quote:
... The cell lysate was centrifuged at 3,000 rpm (Eppendorf #5810R), at 4 °C for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Zhi-hai He, et al.,
bioRxiv - Epidemiology 2019
Quote:
DNA was extracted from spleen tissue using the DNA blood and tissue kit (Tiangen Biotechnique, Beijing, China) according to the manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Quote:
... tissues were lysed in MasterPure tissue lysis solution (EpiCentre) in the presence of proteinase K (2 mg/ml ...
-
No products found
because this supplier's products are not listed.
Amalia Sintou, et al.,
bioRxiv - Immunology 2019
Quote:
... UK) were coated with 50μl per well of 4μg/μl pig heart lysate lysate (Novus Biologicals, Bio-Techne, Abingdon, UK) diluted in PBS overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Quote:
... Tissues were homogenized by a bullet blender (Next Advance) at 4°C according to manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Quote:
... using Calpain-1 from porcine erythrocytes (Calbiochem), at either 0.05U/uL (Low Calpain ...
-
No products found
because this supplier's products are not listed.
Quote:
... tissue culture dishes (Sarstedt, cat. no. 83.3901.300) were coated with 20 µg/mL Laminin (Invitrogen ...