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No products found
because this supplier's products are not listed.
Laura-Oana Albulescu, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... we used the EnzChek™ Phospholipase A2 Assay Kit (#E10217, Fisher Scientific), following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... group IIA secreted phospholipase A2 (hGIIA) inhibitor LY311727 (50 µM; Sigma-Aldrich Ldt.) were used ...
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No products found
because this supplier's products are not listed.
Quote:
... ab32386 against Cyclin A2 (Abcam), ab32053 against Cyclin B1 (Abcam) ...
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No products found
because this supplier's products are not listed.
Yotam E. Bar-Ephraim, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... HLA-A2− PE (BB7.2, Santa Cruz).
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No products found
because this supplier's products are not listed.
Sushil C Regmi, et al.,
bioRxiv - Cell Biology 2019
Quote:
Protease-activated receptor 1 (PAR-1)-activating peptide (TFLLRNPNDK-NH2) was custom synthesized as the C-terminal amide with a purity of > 95% by Genscript (Piscataway, NJ). Scrambled-siRNA (Sc-siRNA ...
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No products found
because this supplier's products are not listed.
Quote:
... cells were washed twice with FACS-buffer consisting of PBS with 2% FCS and stained for HLA-A2 using a FITC mouse anti-human HLA-A2 antibody (BD Pharmingen™, Clone BB7) for 30 min at 4°C ...
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No products found
because this supplier's products are not listed.
Quote:
... or H3R2me2a peptide (Anaspec) were immobilized on 20μL Streptavidin sepharose resin (Thermo ...
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No products found
because this supplier's products are not listed.
Quote:
... recombinant mouse ephrin-A2-Fc (R&D systems cat. no. 8415-A2) or Fc (Millipore Sigma cat ...
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No products found
because this supplier's products are not listed.
Zaw Min Htet, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Activating adaptors were fused to a ZZ-TEV-HaloTag (Promega) on the amino-terminus and inserted into a pET28a expression vector ...
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No products found
because this supplier's products are not listed.
Christopher J Derrick, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... and injected together with an equimolar concentration of trans-activating RNA (tracrRNA, Merck) and Cas9 protein (NEB M0386T ...
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No products found
because this supplier's products are not listed.
Jun Imai, et al.,
bioRxiv - Immunology 2019
Quote:
... All peptides used in peptide loading experiments were obtained from Peptide Institute Inc ...
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No products found
because this supplier's products are not listed.
Miranda P. Collier, et al.,
bioRxiv - Biophysics 2018
Quote:
Peptides were purchased from Biomatik at 99+% purity with acetylated N-termini when the N-terminal residue was Glu80 ...
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No products found
because this supplier's products are not listed.
Quote:
... Bacterial expression used E.coli codon-optimized human Rab constructs created by cloning Rab GTPases lacking the C-terminal cysteines and with the relevant activating or inactivating point mutations (see Table 1) into the vector pGEX6p2 between EcoR1 and XhoI (GE Healthcare Life Sciences). Full-length NDE1 was PCR amplified from the plasmid HG15586 (Stratech Scientific ...
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No products found
because this supplier's products are not listed.
Quote:
Peptides were identified using ProteinPilot 4.1 (SCIEX), searching the LudwigNR database (downloaded from http://apcf.edu.au as at 27 January 2012 ...
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No products found
because this supplier's products are not listed.
Allison H. Williams, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Peptides were purified by OMIX C18 tips (Agilent), dried and redissolved in 30 µl of 0.1% formic acid in water/acetonitrile (98:2 ...
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No products found
because this supplier's products are not listed.
Zaw Min Htet, et al.,
bioRxiv - Biochemistry 2019
Quote:
Activating adaptors containing amino-terminal HaloTags were expressed in BL-21[DE3] cells (New England Biolabs) at OD 0.4-0.6 with 0.1 mM IPTG for 16 hr at 18°C ...
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No products found
because this supplier's products are not listed.
Quote:
... agatoxin (Peptides International), and calmodulin (Sigma-Aldrich).
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No products found
because this supplier's products are not listed.
Quote:
Cultivations for the phospholipase C experiments were performed at 30°C and 150 rpm in a 50 mm amplitude rotary shaker (Eppendorf New Brunswick, Enfield, CT, USA). Twenty milliliters of preculture (SB medium ...
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No products found
because this supplier's products are not listed.
Federico Ceriani, et al.,
bioRxiv - Neuroscience 2018
Quote:
... and the phospholipase C inhibitor U73122 (Tocris Bioscience, UK), was performed using a picospritzer ...
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No products found
because this supplier's products are not listed.
Quote:
... or 1xE1 (ubiquitin activating enzyme, Enzo Life Sciences) and 1x UbcH5a ...
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No products found
because this supplier's products are not listed.
Quote:
... The labeled peptides were further purified and separated from the unlabeled peptides by RP-HPLC using a Jupiter 300 Å C5 column (Phenomenex). The concentration of fluorescent peptides and the efficiency of labeling were determined by measuring the absorbance of the fluorescent dye and the peptides.
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No products found
because this supplier's products are not listed.
Debjani Pal, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Cyclin A2 (BF683) (#6; 1:1000) and Phospho-Chk1Ser345 (133D3) (#15; 1:1000) were obtained from Cell Signaling. HA (#SJ254200 ...
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No products found
because this supplier's products are not listed.
Mathieu Le Gars, et al.,
bioRxiv - Immunology 2019
Quote:
... NK cells were pre-incubated with activating IB4 antibody (IgG2a, gift from Dr. Fabio Malavasi, University of Torino) blocking CD38 (clone AT13/5, Bio-Rad), CD31 (clone WM59 ...
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No products found
because this supplier's products are not listed.
Quote:
... 3XFlag peptide was from APExBIO. Anti-Flag(M2 ...
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No products found
because this supplier's products are not listed.
He Gu, Bridget T. Hughes, Peter J. Espenshade,
bioRxiv - Cell Biology 2018
Quote:
... Eluting peptides were sprayed through 1 μm emitter tip (New Objective) at 2.0 kV directly into the Q-Exactive ...
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No products found
because this supplier's products are not listed.
Quote:
... anti-Hcp (against synthetic peptide, Eurogentec #1510528 (35)) ...
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No products found
because this supplier's products are not listed.
Quote:
... WDR5 blocking peptide (Bethyl) was used to examine WDR5 specificity in 1:1 and 1:2 (antibody ...
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No products found
because this supplier's products are not listed.
Carola Städele, Wolfgang Stein,
bioRxiv - Neuroscience 2015
Quote:
... FMRF-like peptide F1 (TNRNFLRF-NH2, Bachem) and cimetidine hydrochloride (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... Infection with RSV A2 (Advanced Biotechnologies Inc), amplified and purified as described in 37 ...
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No products found
because this supplier's products are not listed.
Lili Qin, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... cells were stained by anti-human HLA-A2 antibody (BB7.2, BioLegend, USA) with PE-labeled ...
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No products found
because this supplier's products are not listed.
Elahe Ganji, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Collected slides were then imaged with an Imager A2 microscope (Carl Zeiss, Germany).
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No products found
because this supplier's products are not listed.
Quote:
... For T cell differentiation: Naïve CD4 T cells were differentiated on activating polystyrene surface (Corning Inc., Corning, NY) with plate-bound αCD3 (2.5 μg/ml ...
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No products found
because this supplier's products are not listed.
Quote:
... Peptides were desalted with C18 UltraMicroSpin columns (The Nest Group) according to the manufacturer’s instructions and dried in a SpeedVac concentrator (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Xiaozhu Wang, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... A2 and B Wolbachia using PyroMark Assay Design 2.0 (Qiagen, USA). A complete list of primers sequences could be found in Table S3 ...
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No products found
because this supplier's products are not listed.
Quote:
... and spinal cords were fixed in 4% PFA and optically cleared by immersion in Scaleview-A2 (Olympus, Tokyo, Japan) at room temperature overnight ...
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No products found
because this supplier's products are not listed.
Quote:
... and mutated to incorporate an activating mutation in the translated protein (F751L) then cloned into pMT-Puro vector (from David Sabatini, Addgene stock 17923). To generate the 5’UTR-crcELuciferase reporter-construct ...
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No products found
because this supplier's products are not listed.
Annie Lee, et al.,
bioRxiv - Neuroscience 2019
Quote:
... a peptide corresponding to the inverted sequence of Aβ42 (INV42; rPeptide and Creative Peptide) was processed as for Aβ42 oligomerization ...
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No products found
because this supplier's products are not listed.
Sofie K. Christensen, et al.,
bioRxiv - Cell Biology 2020
Quote:
... peptide-N-glycosidase F (PNGase F) (Roche), neuraminidase (Roche) ...
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No products found
because this supplier's products are not listed.
Quote:
... Forward genetic screen for constitutive GFP reporter-activating mutants after ethyl methanesulfonate (EMS)-induced random mutagenesis was performed as described previously38,39 ...
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No products found
because this supplier's products are not listed.
Quote:
... the DNA binding domain (DBD) or activating domain (AD)-fused constructs were transformed using the Li-Ac transformation protocol (Clontech) into Saccharomyces cerevisiae strain Y187 (MATα ...
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No products found
because this supplier's products are not listed.
Lei Wang, Hongwu Du, Peng Chen,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Same method was used to detect p50-Annnxin A2 complex with mouse monoclonal antibody of ANXA2 (Proteintech, Wuhan, China), and the p50 antibody was detected by Western blot with Rabbit anti-p50 antibody (Proteintech ...
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No products found
because this supplier's products are not listed.
Quote:
... Peptides were desalted via C18 Macro SpinColumns (Harvard Apparatus) and dried under vacuum centrifugation.
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No products found
because this supplier's products are not listed.
C López-Haber, et al.,
bioRxiv - Cell Biology 2020
Quote:
... OT-II peptide OVA323-339 (ISQAVHAAHAEINEAGR) was from InvivoGen (San Diego, CA) and kindly provided by Paula Oliver (Children’s Hospital of Philadelphia ...
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No products found
because this supplier's products are not listed.
Quote:
... and the METABOLOMICS AMINO ACID MIX STANDARD (contains 13C3, 15N-Serine, cat #MSK-A2-1.2, Cambridge Isotope Laboratories)
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No products found
because this supplier's products are not listed.
Quote:
... and trans-activating crRNA (tracrRNA) (IDT) or single guide RNAs (sgRNA) transcribed in vitro were combined with Cas9 protein (PNA Bio Inc. or IDT) to generate complexes used for genome editing ...
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No products found
because this supplier's products are not listed.
Marcin Poreba, et al.,
bioRxiv - Biochemistry 2019
Quote:
... N,N-diisopropylethylamine (DIPEA, peptide grade), piperidine (PIP, peptide grade), and trifluoroacetic acid (TFA, purity 99%) were all from Iris Biotech. 2,4,6-trimethylpyridine (2,4,6-collidine ...
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No products found
because this supplier's products are not listed.
Alexey Stukalov, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Peptide purification on StageTips with three layers of C18 Empore filter discs (3M) and subsequent mass spectrometry analysis was performed as described previously22,23 ...
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No products found
because this supplier's products are not listed.
Quote:
... Total PC concentration was determined using the Phospholipase C assay kit (Wako Diagnostics).
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No products found
because this supplier's products are not listed.
Quote:
... All tests were performed inside a Class 2 Type A2 Biological Safety Cabinet (NuAire, Plymouth, MN), and all exposure toxicants were ventilated out via the built-in and lab ventilation system.
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No products found
because this supplier's products are not listed.
Eunhee Choi, et al.,
bioRxiv - Cell Biology 2018
Quote:
... and Mouse C-peptide ELISA kit (Alpco), respectively ...