-
No products found
because this supplier's products are not listed.
Quote:
... a blocking FLAG peptide (Sigma), used at a 100 μg/ml concentration ...
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No products found
because this supplier's products are not listed.
Quote:
... After blocking with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific), the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies) ...
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No products found
because this supplier's products are not listed.
Hani Al-Shareef, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... E-cadherin (36/E-Cadherin) and N-cadherin (32/N-Cadherin) were obtained from BD Biosciences (Becton Dickinson ...
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No products found
because this supplier's products are not listed.
Quote:
... blocking solution followed by another 4C overnight incubation with either rabbit anti-human E-cadherin 1:100 (Abcam, ab53033), mouse anti-human phospho-Sp1 1:100 (Abcam ...
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No products found
because this supplier's products are not listed.
Quote:
... After blocking in Odyssey Blocking Buffer (PBS, LI-COR 927), membranes were incubated sequentially with primary and secondary antibodies in Odyssey Blocking Buffer mixed with equal volume of PBS and 0.1% (v/v ...
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No products found
because this supplier's products are not listed.
Quote:
... E-Cadherin (Cell Signaling), HSP90 (Cell Signaling) ...
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No products found
because this supplier's products are not listed.
Quote:
... Embryos were washed in diluted stop solution and PBS-X and then incubated with a blocking solution containing: 2% (w/v) blocking reagent (Roche), 0.15 M NaCl ...
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No products found
because this supplier's products are not listed.
Quote:
... washed 1x in PBS and re-suspended in blocking buffer (PBS/BSA 1% plus TruStain fcX anti-mouse CD16/32 (Biolegend)) on ice for 30 min ...
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No products found
because this supplier's products are not listed.
Sarmistha Mahanty, et al.,
bioRxiv - Cell Biology 2018
Quote:
... followed by an incubation in 1.5% blocking serum in PBS (Santa Cruz Biotechnology, sc-2043) for 1 h at room temperature under moist condition ...
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No products found
because this supplier's products are not listed.
Cora Olpe, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... slides were incubated with blocking buffer (PBS-T with 10% Donkey Serum, Dako) for 30 min ...
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No products found
because this supplier's products are not listed.
Tui Neri, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... VE-cadherin (R&D systems/MAB9381), Sox9 (a gift from Pr Wegner ...
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No products found
because this supplier's products are not listed.
Pujarini Dash, et al.,
bioRxiv - Biochemistry 2018
Quote:
... and then washed with PBS followed by blocking with horse serum (Vector Lab) for 30 min ...
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No products found
because this supplier's products are not listed.
Quote:
... Scrambled (Ac-AGVGDHIGC, to make GelMA-Scram) or N-Cadherin mimic (Ac-HAVDIGGGC, to make GelMA-Cad) peptides (GenScript) were added to the GelMA/TEOA buffer to form a 1% w/v solution ...
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No products found
because this supplier's products are not listed.
Quote:
... WDR5 blocking peptide (Bethyl) was used to examine WDR5 specificity in 1:1 and 1:2 (antibody ...
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No products found
because this supplier's products are not listed.
Amy Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... in PBS followed by blocking with 0.5% PNB in PBS (Perkin Elmer). Primary antibodies were incubated overnight ...
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No products found
because this supplier's products are not listed.
Madel Durens, et al.,
bioRxiv - Neuroscience 2019
Quote:
... SFEBs were washed 3x with PBS and incubated with blocking solution (PBS with 5% Normal Donkey Serum (Jackson Immunoresearch Labs) and 0.1% Triton-X ...
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No products found
because this supplier's products are not listed.
Emma S Chambers, et al.,
bioRxiv - Immunology 2020
Quote:
... After blocking in ECL blocking agent (GE Healthcare), membranes were probed with primary antibodies overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Quote:
... Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck; blocking buffer) and incubated with primary antibodies (Table S1 ...
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No products found
because this supplier's products are not listed.
E Chuntharpursat-Bon, et al.,
bioRxiv - Cell Biology 2019
Quote:
... mVenus-VE-Cadherin-N-10 (Addgene plasmid # 56340 ...
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No products found
because this supplier's products are not listed.
Jun Imai, et al.,
bioRxiv - Immunology 2019
Quote:
... All peptides used in peptide loading experiments were obtained from Peptide Institute Inc ...
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No products found
because this supplier's products are not listed.
Quote:
... The membranes were blocked 1 hr at room temperature with Odyssey PBS Blocking Buffer (Li-Cor, 927-40000) or in 5% Blotting-Grade Blocker (Biorad, 1706404) in TBST (50 mM Tris buffer ...
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No products found
because this supplier's products are not listed.
Quote:
... or H3R2me2a peptide (Anaspec) were immobilized on 20μL Streptavidin sepharose resin (Thermo ...
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No products found
because this supplier's products are not listed.
Defne Bayik, et al.,
bioRxiv - Pathology 2019
Quote:
... and blocked in FACS buffer (PBS, 2% BSA) containing an FcR blocking reagent (Miltenyi Biotec). Samples were stained with fluorophore-conjugated antibody cocktails ...
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No products found
because this supplier's products are not listed.
Miranda P. Collier, et al.,
bioRxiv - Biophysics 2018
Quote:
Peptides were purchased from Biomatik at 99+% purity with acetylated N-termini when the N-terminal residue was Glu80 ...
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No products found
because this supplier's products are not listed.
Quote:
... and blocking with TBS/Blocking buffer (Rockland), the membrane was probed with anti-DmNMNAT (1:8,000 ...
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No products found
because this supplier's products are not listed.
Quote:
Peptides were identified using ProteinPilot 4.1 (SCIEX), searching the LudwigNR database (downloaded from http://apcf.edu.au as at 27 January 2012 ...
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No products found
because this supplier's products are not listed.
Ekaterina V. Tarasovetc, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Coverslips were washed three times in 0.1% Tween-20 in Corning PBS and incubated with blocking buffer (Corning PBS containing 2% fetal bovine serum ...
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No products found
because this supplier's products are not listed.
Erik Johnson, et al.,
bioRxiv - Systems Biology 2020
Quote:
... and an external reference peptide mix (Promega) was spiked into the sample at the concentration of 0.5 pmol/µl ...
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No products found
because this supplier's products are not listed.
Quote:
... agatoxin (Peptides International), and calmodulin (Sigma-Aldrich).
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No products found
because this supplier's products are not listed.
Quote:
... Digestions with Peptide-N-Glycosidase F (PNGaseF, NEB) were carried out for 4h at 37°C in the presence of 1% NP40 and the buffer provided by the manufacturer (NEB) ...
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No products found
because this supplier's products are not listed.
Quote:
... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...
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No products found
because this supplier's products are not listed.
Quote:
... and cadherin-17 (NBP2-12065AF488; Novus Biologicals; 1:200) for 1 hour in 1% BSA in PBS ...
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No products found
because this supplier's products are not listed.
Elisabetta Prina, et al.,
bioRxiv - Bioengineering 2018
Quote:
... Blocking solution was composed of PBS containing 1% (w/v) of Bovine Serum Albumin (BSA, BDH Prolabo®, VWR, USA, 421501J), 0.3 M glycine (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... Peptides were dried and ACN was removed from the peptide containing sample by SpeedVac (Eppendorf, Germany) at 45°C ...
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No products found
because this supplier's products are not listed.
Quote:
... The labeled peptides were further purified and separated from the unlabeled peptides by RP-HPLC using a Jupiter 300 Å C5 column (Phenomenex). The concentration of fluorescent peptides and the efficiency of labeling were determined by measuring the absorbance of the fluorescent dye and the peptides.
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No products found
because this supplier's products are not listed.
Quote:
... ∼2.5 mg of the labelled peptide mixture were used to enrich for phospho-peptides via immobilized metal affinity chromatography (IMAC) using magnetic beads (Qiagen, Valencia, CA), according to the published method [113] ...
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No products found
because this supplier's products are not listed.
Satyamaanasa Polubothu, et al.,
bioRxiv - Genomics 2019
Quote:
... Slides were washed three times with PBS and incubated with blocking solution (1% Bovine serum albumin, 10% Donkey serum, 0.3M Glycine, 0.1% PBS Tween) for 1 hour at room temperature followed by primary antibody in blocking solution overnight at 4 degrees (see Table 1 for source ...
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No products found
because this supplier's products are not listed.
Quote:
... Peptides were dissolved at 500 μM in buffer (1X Ca+2/Mg+2-free PBS; Lonza, catalogue #17517Q). Mosquitoes were allowed to recover in groups for 12-16 hours at 25-28°C ...
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No products found
because this supplier's products are not listed.
Yury Bykov, et al.,
bioRxiv - Cell Biology 2019
Quote:
... blocked for 30 minutes in filtered blocking solution containing: 0.5% gelatin (EMS), 0.5% BSA (MP biomedical ...
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No products found
because this supplier's products are not listed.
He Gu, Bridget T. Hughes, Peter J. Espenshade,
bioRxiv - Cell Biology 2018
Quote:
... Eluting peptides were sprayed through 1 μm emitter tip (New Objective) at 2.0 kV directly into the Q-Exactive ...
-
No products found
because this supplier's products are not listed.
M. M. Cooley, et al.,
bioRxiv - Pathology 2020
Quote:
... Blocking and incubations were done in PBS supplemented with 3% bovine serum albumin (Calbiochem), 2% goat serum (Sigma) ...
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No products found
because this supplier's products are not listed.
Daehoon Lee, et al.,
bioRxiv - Neuroscience 2018
Quote:
... rabbit anti-phospho VE-cadherin (Y731, GeneTex), rabbit anti-phospho VE-cadherin (Y685 ...
-
No products found
because this supplier's products are not listed.
Quote:
... Cadherin-17 (CDH17) from Proteintech (Rosemont, IL), β-Galactosidase from Promega (Madison ...
-
No products found
because this supplier's products are not listed.
Jia Zhao, et al.,
bioRxiv - Developmental Biology 2017
Quote:
... or 20 VIVIT peptide (Tocris) was used to activate or inhibit NFAT ...
-
No products found
because this supplier's products are not listed.
Corey Tan, et al.,
bioRxiv - Immunology 2020
Quote:
... anti-CD40L blocking Ab (clone MR-1, BioXcell).
-
No products found
because this supplier's products are not listed.
Quote:
... anti-Hcp (against synthetic peptide, Eurogentec #1510528 (35)) ...
-
No products found
because this supplier's products are not listed.
Quote:
... The sample was then applied three times over 0.5 ml of PBS-equilibrated peptide M agarose (Invivogen). The flowthrough after the third pass was collected as IgA-depleted plasma ...
-
No products found
because this supplier's products are not listed.
Quote:
... Rabbit polyclonal antibodies used were: HECD1 (anti–E-cadherin; Takara, Kyoto, Japan), Flag (Cell Signaling Technologies) ...
-
No products found
because this supplier's products are not listed.
Luise Zuehl, David Ibberson, Anja Schmidt,
bioRxiv - Plant Biology 2018
Quote:
... Prior to blocking in Paraplast X-tra (Leica Biosystems, Nussloch, Germany), flower buds were incubated first overnight ...
-
No products found
because this supplier's products are not listed.
Quote:
... Slides were then washed in PBS + 0.1% Triton 3 × 10 min and PBS 1 × 5 min then coverslipped with Fluoromount G (Southern biotech Cat OB10001) under glass cover slips ...