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No products found
because this supplier's products are not listed.
Quote:
... a blocking FLAG peptide (Sigma), used at a 100 μg/ml concentration ...
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No products found
because this supplier's products are not listed.
Quote:
... 5% blocking reagent (Roche) containing 2.5 μg/mL Cy-3-labelled telomere specific (CCCTAA ...
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No products found
because this supplier's products are not listed.
Quote:
... or PAK4 inhibitor (vandetanib, catalogue # NC0706691, Thermo Fisher Scientific), alone or in combination ...
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No products found
because this supplier's products are not listed.
Quote:
... followed by blocking with 5% BSA (BIO-RAD, Hercules, CA) at room temperature for 1 h ...
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No products found
because this supplier's products are not listed.
Quote:
... Blocking solution was 5% horse serum (Vector Laboratories) in Triton 0.5% and primary antibodies were incubated for at least 2 days ...
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No products found
because this supplier's products are not listed.
Mo-Xian Chen, et al.,
bioRxiv - Plant Biology 2018
Quote:
... Peptides were first loaded onto a C18 trap column (5 μm, 5 x 0.3 mm, Agilent Technologies) and then eluted into a C18 analytical column (75 μm × 150 mm ...
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No products found
because this supplier's products are not listed.
Quote:
... 5 µg/ml TSKB20 (ANYKFTLV) peptide (Genscript Inc.) or 50 ng/mL PMA plus 500 ng/ml ionomycin (Sigma ...
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No products found
because this supplier's products are not listed.
Quote:
... After blocking with Odyssey blocking buffer (LI-COR) or skim milk for 1 h ...
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No products found
because this supplier's products are not listed.
Quote:
... anti-PAK4 (Cell Signaling, #3242), β-actin (Sigma ...
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No products found
because this supplier's products are not listed.
Israel C. Vasconcelos, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Tryptic peptides were successively extracted with 5 % formic acid (Merck)/50 % acetonitrile (Merck) ...
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No products found
because this supplier's products are not listed.
Weiling He, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Human CD96 blocking antibody (5 μg/ml) or isotype control (Biolegend, USA) was included in some experiments.
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No products found
because this supplier's products are not listed.
Quote:
... WDR5 blocking peptide (Bethyl) was used to examine WDR5 specificity in 1:1 and 1:2 (antibody ...
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No products found
because this supplier's products are not listed.
Quote:
... the blocking solution was supplemented with 5 mg/liter of anti-integrin β1 blocking antibody P5D2 (Abcam, San Francisco, CA), low affinity locking anti-integrin β1 antibody SG19 ...
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No products found
because this supplier's products are not listed.
Kohta Miyawaki, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... followed by blocking in 5% bovine serum albumin (Jackson ImmunoResearch, Birmingham, AL, USA) in PBS ...
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No products found
because this supplier's products are not listed.
Quote:
... and blocked overnight in 5% w/w ECL blocking agent (GE Healthcare) in TTBS (0.05% Tween 20 in Tris buffered saline) ...
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No products found
because this supplier's products are not listed.
Tsuyoshi Waku, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... After the treatment with 5 nM or 10 nM bortezomib (BTZ, Peptide Institute), the cells were stained by Trypan Blue and automatically counted using a TC20 Automated Cell Counter (Bio-Rad Laboratories).
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No products found
because this supplier's products are not listed.
Sebastian Sorge, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... Blocking was done with 1% blocking reagent (PerkinElmer) in PBT (0.1% Tween-20) ...
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No products found
because this supplier's products are not listed.
Quote:
... and FcR blocking reagent (Miltenyi Biotec, dilution 1:5) or isotype control antibody (mouse IgG1 ...
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No products found
because this supplier's products are not listed.
J.A.M. Bard, C. Bashore, K.C. Dong, A. Martin,
bioRxiv - Biochemistry 2018
Quote:
... peptide (HHHHHHLPETGG) was purchased with 5-FAM conjugated to its N-terminus (Biomatik), and then ligated to 100 μM substrate using 20 μM recombinant 6xHis-SrtA in GF buffer supplemented with 10 mM CaCl2 ...
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No products found
because this supplier's products are not listed.
Patricia E. Collins, et al.,
bioRxiv - Immunology 2018
Quote:
... For endogenous immunoprecipitation experiments TPL-2 was eluted using the TPL-2 blocking peptide by incubation at room temperature for 10 minutes with occasional agitation (Santa Cruz). Equal volumes of resuspended immunoprecipitates were analysed by western blot ...
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No products found
because this supplier's products are not listed.
Quote:
... or H3R2me2a peptide (Anaspec) were immobilized on 20μL Streptavidin sepharose resin (Thermo ...
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No products found
because this supplier's products are not listed.
Yoong Wearn Lim, et al.,
bioRxiv - Genomics 2020
Quote:
... The liquid was poured off from the cells (leaving approximately 100 µl in each tube) and 5 µl of mouse Fc Blocking Reagent (rat anti-Mouse CD16/CD32 BD) was added to samples and incubated for 5 minutes at 4°C ...
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No products found
because this supplier's products are not listed.
Shady Younis, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Instrument quality control was monitored using the Promega 6×5 LC-MS/MS Peptide Reference Mix (Promega) before and after each MS experiment run ...
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No products found
because this supplier's products are not listed.
Quote:
... Dried peptides were suspended in 5% (v/v) formic acid and analysed on a TripleTOF 5600 mass spectrometer (SCIEX) in-line with a nanoflow electrospray ion source and nano-HPLC system ...
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No products found
because this supplier's products are not listed.
Quote:
... 5% TFA) was added to eluted peptide mixture in Protein LoBind 96 well plate (Eppendorf) at 1:1 volume ratio ...
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No products found
because this supplier's products are not listed.
S. Okada, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
Reduced and alkylated tryptic peptides (5 μL) were chromatographically separated on a Kinetex C18 column (2.1 mm x 100 mm, Phenomenex) using a linear gradient of 5–45% acetonitrile (in 0.1% formic acid ...
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No products found
because this supplier's products are not listed.
Quote:
... and blocking with TBS/Blocking buffer (Rockland), the membrane was probed with anti-DmNMNAT (1:8,000 ...
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No products found
because this supplier's products are not listed.
Quote:
... Digestions with Peptide-N-Glycosidase F (PNGaseF, NEB) were carried out for 4h at 37°C in the presence of 1% NP40 and the buffer provided by the manufacturer (NEB) ...
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No products found
because this supplier's products are not listed.
Quote:
... blocking anti-CXCR4 MAB21651 (247506, R&D Systems) or anti-CCR7 MAB3477 (4B12 ...
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No products found
because this supplier's products are not listed.
Quote:
... agatoxin (Peptides International), and calmodulin (Sigma-Aldrich).
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No products found
because this supplier's products are not listed.
Jia Zhao, et al.,
bioRxiv - Developmental Biology 2017
Quote:
... or 20 VIVIT peptide (Tocris) was used to activate or inhibit NFAT ...
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No products found
because this supplier's products are not listed.
Quote:
... 3XFlag peptide was from APExBIO. Anti-Flag(M2 ...
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No products found
because this supplier's products are not listed.
Weiling Hong, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... After blocking with 5% BSA (VWR LIFE SCIENCE) for 1 h at room temperature ...
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No products found
because this supplier's products are not listed.
Quote:
... chromatographic separation of peptides was performed on 20-cm emitter (75 μm inner diameter, #PF360-75-10-N-5, New Objective) packed in-house with C18 resin (Magic C18 AQ 3 μm diameter ...
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No products found
because this supplier's products are not listed.
Jenny Clarke, et al.,
bioRxiv - Microbiology 2019
Quote:
... After blocking (Peprotech), samples were added to the wells and incubated for 2 h at room temperature ...
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No products found
because this supplier's products are not listed.
Quote:
... TAM-treated K14CreERT;Ptchf/f mice were intraperitoneally injected every third day for 5 weeks with 250 µg of anti-PD-1 blocking antibodies (clone RMP1-14, BioXCell, West Lebanon, NH), according to previously published papers (Curran et al. ...
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No products found
because this supplier's products are not listed.
Quote:
... anti-Hcp (against synthetic peptide, Eurogentec #1510528 (35)) ...
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No products found
because this supplier's products are not listed.
Quote:
... ∼2.5 mg of the labelled peptide mixture were used to enrich for phospho-peptides via immobilized metal affinity chromatography (IMAC) using magnetic beads (Qiagen, Valencia, CA), according to the published method [113] ...
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No products found
because this supplier's products are not listed.
Quote:
... The digestion was quenched with 5% formic acid (final concentration) and resulting peptide mixtures were desalted using in-house made C18 Empore (3M) StAGE tips (Rappsilber et al. ...
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No products found
because this supplier's products are not listed.
Quote:
... combinations of peptides and antibiotics were prepared in 96 well plates (polypropylene, Corning) in a twofold dilution series ...
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No products found
because this supplier's products are not listed.
Quote:
... 5’-ATCGAGTGCGATCACAAGCAG-3’ (1st PCR) and 5’-ACTCTCAGATTCCGGATTCACC-3’ (2nd PCR) using 5’-Full RACE Kit (TAKARA). For the 3’ RACE ...
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No products found
because this supplier's products are not listed.
Quote:
... 100 µM TIRAP (TLR2 and TLR4) inhibitor peptide or control peptide (Novus Biologicals) were added to neutrophils at 1 h before incubation.
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No products found
because this supplier's products are not listed.
Quote:
... OT-II peptide OVA323-339 (ISQAVHAAHAEINEAGR) was from InvivoGen (San Diego, CA) and kindly provided by Paula Oliver (Children’s Hospital of Philadelphia ...
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No products found
because this supplier's products are not listed.
Quote:
... mNG21-104 fused to the self-cleaving 2A peptide and tdTomato (Addgene #37347) was cloned into the lenti dCAS-VP64_Blast (Addgene #61425 ...
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No products found
because this supplier's products are not listed.
Quote:
... The membrane was treated with blocking buffer (5% EasyBlocker (GeneTex) in TBS ...
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No products found
because this supplier's products are not listed.
Quote:
The blocking of β1 integrin activity was done using 4B4 β1 integrin blocking antibody (Beckman Coulter) at doses of 0 ...
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No products found
because this supplier's products are not listed.
Quote:
Modified histone peptide arrays (Active Motif) were blocked with 5% dried milk in TBS-T (10 mM Tris-HCl pH 7.6 ...
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No products found
because this supplier's products are not listed.
Carola Städele, Wolfgang Stein,
bioRxiv - Neuroscience 2015
Quote:
... FMRF-like peptide F1 (TNRNFLRF-NH2, Bachem) and cimetidine hydrochloride (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Nicolas Vabret, et al.,
bioRxiv - Immunology 2019
Quote:
1ug of total cellular RNA was treated with RNA 5’ polyphosphatase (enzyme that converts 5’-triphosphorylated RNA into 5’-monophosphorylated RNA, Lucigen) for 30 min at 37°C or mock-treated ...
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No products found
because this supplier's products are not listed.
Quote:
... Upon clean up with DNA Clean & Concentrator-5 (DCC-5) kit (Zymo Research), this PCR was followed by another bulk up PCR using the same program ...