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PAK1 2 3 Blocking Peptide
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Cohesion Biosciences Sponsored
The peptide is used to block Anti-PAK1/2/3 Antibody (#CPA4765) reactivity.Cat# CBP4765, 1 mg USD $100.0, 5 mg USD $300.0 Ask
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Roche
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2019Quote: ... 2% blocking reagent (Roche), 20% heat-inactivated goat serum and then incubated overnight with anti-DIG antibody (Roche ... -
Millipore Sigma
No products found because this supplier's products are not listed.Cited in Endolysosomal degradation of Tau and its role in glucocorticoid-driven hippocampal malfunctionbioRxiv - Neuroscience 2018, published in The EMBO Journal doi: 10.15252/embj.201899084Quote: ... a blocking FLAG peptide (Sigma), used at a 100 μg/ml concentration ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.Cited in The TRPC6-AMPK pathway is involved in cytoskeleton reorganization and glucose uptake in podocytesbioRxiv - Cell Biology 2018, published in Cellular Physiology and Biochemistry doi: 10.1159/000495236Quote: ... anti-PAK1/2/3 (1:800, Cell Signaling Technology), anti-ROCK1 (1:1000 ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... Peptides were desalted (Acclaim PepMap 100, 75µm × 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA) and separated (Acclaim ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Physiology 2018, published in The Journal of Experimental Biology doi: 10.1242/jeb.185884Quote: ... melanogaster CAPA2 (DromeCAPA-2: ASGLVAFPRV-NH2) peptide and an N-terminally biotinylated DromeCAPA2 (biotin-CAPA-2) peptide analog (Genscript Inc., Piscataway, NJ) were prepared in block solution ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... For endogenous immunoprecipitation experiments TPL-2 was eluted using the TPL-2 blocking peptide by incubation at room temperature for 10 minutes with occasional agitation (Santa Cruz). Equal volumes of resuspended immunoprecipitates were analysed by western blot ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... Specificity of the antibody was tested incubating part of the membrane with the blocking peptide (1 μg/mL; Abcam, ab66772). Chemiluminescence reagents (Supersignal West Pico Chemiluminescence substrate ... -
LI-COR
No products found because this supplier's products are not listed.Cited in Cell-to-cell heterogeneity in p38-mediated cross-inhibition of JNK causes stochastic cell deathbioRxiv - Cell Biology 2018, published in Cell Reports doi: 10.1016/j.celrep.2018.08.020Quote: ... After blocking with Odyssey blocking buffer (LI-COR) or skim milk for 1 h ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Genomics 2019Quote: ... Blocking was performed by 1h incubation in humid chamber with 3% of normal donkey serum (Vendor) in blocking solution (DAKO). Slides were incubated in humid chamber with primary antibodies overnight at 4°C and washed in PBS 0.2% Triton-X100 ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2017Quote: ... was assessed using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent (Promega) following the supplier’s protocol ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Physiology 2019Quote: ... Upon blocking the membrane with 2% (w/v) Amersham ECL Prime Blocking Reagent (GE Healthcare) for an hour at room temperature ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Physiology 2020Quote: ... Live bone marrow cells were subject to FcR-receptor blocking (1:3; BioLegend; Cat. No. 101319) and viability staining (1:500 ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2016, published in Cancer Cell doi: 10.1016/j.ccell.2017.06.003Quote: ... Peroxidase block was conducted with 3% H2O2 and blocking was with 5% goat serum (Vector Labs #S-1000). Primary rabbit polyconal antibody against IBA1 (AIF1)(WAKO #016-20001 ... -
Bethyl
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2017, published in Developmental Cell doi: 10.1016/j.devcel.2018.08.009Quote: ... WDR5 blocking peptide (Bethyl) was used to examine WDR5 specificity in 1:1 and 1:2 (antibody ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2017, published in PLOS Genetics doi: 10.1371/journal.pgen.1007073Quote: ... pGEX-2T-GST Cdc42WT was obtained from Addgene (#12969) and pGEXTK-Pak1 (70-117) was obtained from Addgene (#12117). pRKVSV-Cdc42 WT ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2019Quote: ... After blocking with 2% normal donkey serum (Jackson ImmunoResearch), 0.1% Triton in PBS for 30 min at room temperature ... -
Avanti Polar Lipids
No products found because this supplier's products are not listed.Cited in The lipid droplet protein Pgc1 controls the subcellular distribution of phosphatidylglycerolbioRxiv - Cell Biology 2018, published in FEMS Yeast Research doi: 10.1093/femsyr/foz045Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-oleoyl-sn-3-phosphoethanolamine (3:1; Avanti Polar Lipids) were dissolved in chloroform ... -
PerkinElmer
No products found because this supplier's products are not listed.Cited in Microstimulation of primate neocortex targeting striosomes induces negative decision-makingbioRxiv - Neuroscience 2019, published in European Journal of Neuroscience doi: 10.1111/ejn.14555Quote: ... Sections were rinsed 3 times for 2 min in PBS-Tx and incubated in tyramide signal amplification (TSA) blocking reagent (PerkinElmer, FP1012) in PBS-Tx (TSA-block ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: ... and eluted by incubation with 3× Flag peptide (Merck). A cDNA of StrepTagII-tagged PTBP1 was cloned into pET49 plasmid ... -
Anaspec
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in JCI Insight doi: 10.1172/jci.insight.130090Quote: ... (2 μg/ml/peptide, ANASPEC). Results are reported as spot-forming cells (SFC ... -
Peptide Institute
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0200432Quote: ... cells were stimulated for 3 h with ConA (2 μg/ml) with or without CGRP (1 or 10 nM) (Peptide Institute) in RPMI 1640 medium ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019Quote: ... The lyophilized peptides were resuspended in 200 µl of 50 mM ammonium bicarbonate to which 3 µl of PNGaseF (New England Biolabs) were added for a 4h incubation at 37C ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in ACS Infectious Diseases doi: 10.1021/acsinfecdis.9b00412Quote: ... followed by blocking with 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Membranes were incubated with streptavidin conjugated to horse radish peroxidase (1:7000 ... -
ApexBio
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in Nature Chemical Biology doi: 10.1038/s41589-019-0304-8Quote: ... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ... -
Lonza
No products found because this supplier's products are not listed.Cited in HMGB1 coordinates SASP-related chromatin folding and RNA homeostasis on the path to senescencebioRxiv - Genomics 2020Quote: ... donors (passage 2-3; Lonza) were continuously passaged to replicative exhaustion in complete Endopan-2 supplemented with 2% FBS under 5% CO2 ... -
Phenomenex
No products found because this supplier's products are not listed.Cited in FlashPack: Fast and simple preparation of ultra-high performance capillary columns for LC-MSbioRxiv - Biochemistry 2018, published in Molecular & Cellular Proteomics doi: 10.1074/mcp.tir118.000953Quote: ... Luna 2 C18 3 μm (Phenomenex), Zorbax SB-C18 1.8 μm (Agilent) ... -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: Signal pathway involvement was investigated by pharmacologically blocking human epidermal growth factor receptor 2 (EGFR 2/ErbB2; TAK 165, 366017-09-6; Tocris Bioscience), formyl peptide receptor-like 1(FPRL1 ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2017, published in Clinical Epigenetics doi: 10.1186/s13148-017-0391-xQuote: ... SiRNAs against Nipbl (Nipbl-1: 5’-GTGGTCGTTACCGAAACCGAA-3’; Nipbl-2: 5’-AAGGCAGTACTTAGACTTTAA-3’) and Rad21 (5’-CTCGAGAATGGTAATTGTATA-3’) were made by Qiagen. AllStars Negative Control siRNA was obtained from Qiagen. -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Pathology 2019Quote: ... and blocked in FACS buffer (PBS, 2% BSA) containing an FcR blocking reagent (Miltenyi Biotec). Samples were stained with fluorophore-conjugated antibody cocktails ... -
Sciex
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018Quote: ... Peptides were separated using a 15 cm chip column (ChromXP C18, 3 μm, 120 Å)(SCIEX). The mass spectrometer was operated in positive ion mode using a data dependent acquisition method (DDA ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Blocking with mouse Fc Block (BD Biosciences, catalogue# 553141) was performed for 10 minutes at 4°C and either or both of mouse anti-PE (clone PE001 ... -
Biomatik Corporation
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in PLOS Pathogens doi: 10.1371/journal.ppat.1006982Quote: ... The production of titan cells was also assessed in the presence of various reagents added at step 3: (1) Qsp1 peptide (NFGAPGGAYPW, [33]) (Biomatik, Cambridge, Canada) was resuspended at 10mM in water and stored at −80°C until use at 10 µM final with the scrambled peptide (AYAPWFGNPG ... -
R&D Systems
No products found because this supplier's products are not listed.Cited in High rates of apoptosis visualized in the symbiont-bearing gills of deep-sea Bathymodiolus musselsbioRxiv - Zoology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0211499Quote: ... Tissue sections were incubated with the rabbit polyclonal active anti-Caspase-3 primary antibody (directed against a peptide from the P17 fragment of the activated human/mouse Caspase-3) (R&D system, USA). This primary incubation lasted 2 hours at 4°C ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019Quote: ... After blocking (Peprotech), samples were added to the wells and incubated for 2 h at room temperature ... -
Eurogentec
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2017Quote: Biotinylated peptides (synthetized by Eurogentec, sequences shown in Figure 3) were quite insoluble in water and were resuspended in 6M urea ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... The peptide rOv-GRN-1was concentrated using Centripep with cut-off 3 kDa (Eppendorf) and resuspended in low salt solution ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2018, published in GigaScience doi: 10.1093/gigascience/giy126Quote: ... we used microscopy system 3 (Leica DMi8, Table 2). -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... 2’,3’-cGAMP (Invivogen) at 10 μg/mL ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Pathology 2020Quote: ... Blocking and incubations were done in PBS supplemented with 3% bovine serum albumin (Calbiochem), 2% goat serum (Sigma) ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2018Quote: ... and passaged every 2–3 days with 0.25% trypsin (Corning# 25-053-CI) to maintain subconfluency ... -
Rockland
No products found because this supplier's products are not listed.Cited in MicroRNA miR-1002 enhances NMNAT-mediated stress response by modulating alternative splicingbioRxiv - Neuroscience 2019, published in iScience doi: 10.1016/j.isci.2019.08.052Quote: ... and blocking with TBS/Blocking buffer (Rockland), the membrane was probed with anti-DmNMNAT (1:8,000 ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in The Journal of Neuroscience doi: 10.1523/JNEUROSCI.1514-19.2020Quote: ... Novaplus) followed by freshly prepared 2% glutaraldehyde/2% paraformaldehyde in 0.1M PB solution filtered with #3 filter paper (VWR) and pH to 7.4 ... -
Quantifoil Micro Tools
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Journal of Cell Biology doi: 10.1083/jcb.201809088Quote: Dam1C/DASH-MT complexes (3 µl) were applied on the carbon side of a Quantifoil 2/2 grid (Quantifoil Micro Tools GmbH ... -
Illumina
No products found because this supplier's products are not listed.Cited in Terminal uridylyltransferases target RNA viruses as part of the innate immune system in animalsbioRxiv - Immunology 2017Quote: ... 2 μg of RNA were submitted to 3′ ligation using the TruSeq Small RNA kit (Illumina), following the manufacturer’s instructions ... -
Active Motif
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2019Quote: ... MEIS1/2/3 (Active Motif 39796) and LTL (Vectorshield FL-1321) ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1911024116Quote: ... 2-to-3-month-old C57BL/6J female mice (Jackson Laboratory) were used for fluorescent-minus-one (FMO ... -
Bio X Cell
No products found because this supplier's products are not listed.bioRxiv - Genetics 2019, published in Journal of Virology doi: 10.1128/JVI.01034-19Quote: CC mice received 2 mg of IFNAR-blocking mouse mAb (MAR1-5A3, BioXCell) by intraperitoneal (IP ... -
Cayman Chemical
No products found because this supplier's products are not listed.Cited in Influenza A matrix protein M1 induces lipid membrane deformation via protein multimerizationbioRxiv - Biophysics 2019, published in Bioscience Reports doi: 10.1042/BSR20191024Quote: ... 2-(4-(3-(4-acetyl-3-hydroxy-2-propylphenoxy) propoxy) phenoxy acetic acid (PHE) was purchased from Cayman Chemical (Ann Arbor, MI, USA). 10-fold concentrated phosphate buffer (PBS ... -
Peptides International
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2017, published in Neuron doi: 10.1016/j.neuron.2017.12.032Quote: ... agatoxin (Peptides International), and calmodulin (Sigma-Aldrich). -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in The EMBO Journal doi: 10.15252/embj.2019102586Quote: ... pure resin at 30°C for 2 × 12 h and pure resin with 3% accelerator (BDMA, EMS) at 30°C for 12 h ...