-
No products found
because this supplier's products are not listed.
Ionel Sandovici, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... 2% blocking reagent (Roche), 20% heat-inactivated goat serum and then incubated overnight with anti-DIG antibody (Roche ...
-
No products found
because this supplier's products are not listed.
Quote:
... a blocking FLAG peptide (Sigma), used at a 100 μg/ml concentration ...
-
No products found
because this supplier's products are not listed.
Quote:
... anti-PAK1/2/3 (1:800, Cell Signaling Technology), anti-ROCK1 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Christopher J. Brown, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Peptides were desalted (Acclaim PepMap 100, 75µm × 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA) and separated (Acclaim ...
-
No products found
because this supplier's products are not listed.
Quote:
... melanogaster CAPA2 (DromeCAPA-2: ASGLVAFPRV-NH2) peptide and an N-terminally biotinylated DromeCAPA2 (biotin-CAPA-2) peptide analog (Genscript Inc., Piscataway, NJ) were prepared in block solution ...
-
No products found
because this supplier's products are not listed.
Patricia E. Collins, et al.,
bioRxiv - Immunology 2018
Quote:
... For endogenous immunoprecipitation experiments TPL-2 was eluted using the TPL-2 blocking peptide by incubation at room temperature for 10 minutes with occasional agitation (Santa Cruz). Equal volumes of resuspended immunoprecipitates were analysed by western blot ...
-
No products found
because this supplier's products are not listed.
Ilja Vietor, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Specificity of the antibody was tested incubating part of the membrane with the blocking peptide (1 μg/mL; Abcam, ab66772). Chemiluminescence reagents (Supersignal West Pico Chemiluminescence substrate ...
-
No products found
because this supplier's products are not listed.
Mark Kalisz, et al.,
bioRxiv - Genomics 2019
Quote:
... Blocking was performed by 1h incubation in humid chamber with 3% of normal donkey serum (Vendor) in blocking solution (DAKO). Slides were incubated in humid chamber with primary antibodies overnight at 4°C and washed in PBS 0.2% Triton-X100 ...
-
No products found
because this supplier's products are not listed.
Quote:
... After blocking with Odyssey blocking buffer (LI-COR) or skim milk for 1 h ...
-
No products found
because this supplier's products are not listed.
Sonia Zicari, et al.,
bioRxiv - Molecular Biology 2017
Quote:
... was assessed using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent (Promega) following the supplier’s protocol ...
-
No products found
because this supplier's products are not listed.
Chitra Devi Ramachandran, et al.,
bioRxiv - Physiology 2019
Quote:
... Upon blocking the membrane with 2% (w/v) Amersham ECL Prime Blocking Reagent (GE Healthcare) for an hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Chen Hao Lo, et al.,
bioRxiv - Physiology 2020
Quote:
... Live bone marrow cells were subject to FcR-receptor blocking (1:3; BioLegend; Cat. No. 101319) and viability staining (1:500 ...
-
No products found
because this supplier's products are not listed.
Quote:
... Peroxidase block was conducted with 3% H2O2 and blocking was with 5% goat serum (Vector Labs #S-1000). Primary rabbit polyconal antibody against IBA1 (AIF1)(WAKO #016-20001 ...
-
No products found
because this supplier's products are not listed.
Quote:
... WDR5 blocking peptide (Bethyl) was used to examine WDR5 specificity in 1:1 and 1:2 (antibody ...
-
No products found
because this supplier's products are not listed.
Quote:
... pGEX-2T-GST Cdc42WT was obtained from Addgene (#12969) and pGEXTK-Pak1 (70-117) was obtained from Addgene (#12117). pRKVSV-Cdc42 WT ...
-
No products found
because this supplier's products are not listed.
Céline Portal, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... After blocking with 2% normal donkey serum (Jackson ImmunoResearch), 0.1% Triton in PBS for 30 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Quote:
... 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-oleoyl-sn-3-phosphoethanolamine (3:1; Avanti Polar Lipids) were dissolved in chloroform ...
-
No products found
because this supplier's products are not listed.
Quote:
... Sections were rinsed 3 times for 2 min in PBS-Tx and incubated in tyramide signal amplification (TSA) blocking reagent (PerkinElmer, FP1012) in PBS-Tx (TSA-block ...
-
No products found
because this supplier's products are not listed.
Jun Ogata, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... and eluted by incubation with 3× Flag peptide (Merck). A cDNA of StrepTagII-tagged PTBP1 was cloned into pET49 plasmid ...
-
No products found
because this supplier's products are not listed.
Quote:
... (2 μg/ml/peptide, ANASPEC). Results are reported as spot-forming cells (SFC ...
-
No products found
because this supplier's products are not listed.
Quote:
... cells were stimulated for 3 h with ConA (2 μg/ml) with or without CGRP (1 or 10 nM) (Peptide Institute) in RPMI 1640 medium ...
-
No products found
because this supplier's products are not listed.
ling huang, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... The lyophilized peptides were resuspended in 200 µl of 50 mM ammonium bicarbonate to which 3 µl of PNGaseF (New England Biolabs) were added for a 4h incubation at 37C ...
-
No products found
because this supplier's products are not listed.
Quote:
... followed by blocking with 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Membranes were incubated with streptavidin conjugated to horse radish peroxidase (1:7000 ...
-
No products found
because this supplier's products are not listed.
Quote:
... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...
-
No products found
because this supplier's products are not listed.
Konstantinos Sofiadis, et al.,
bioRxiv - Genomics 2020
Quote:
... donors (passage 2-3; Lonza) were continuously passaged to replicative exhaustion in complete Endopan-2 supplemented with 2% FBS under 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Quote:
... Luna 2 C18 3 μm (Phenomenex), Zorbax SB-C18 1.8 μm (Agilent) ...
-
No products found
because this supplier's products are not listed.
Ravi Holani, et al.,
bioRxiv - Immunology 2018
Quote:
Signal pathway involvement was investigated by pharmacologically blocking human epidermal growth factor receptor 2 (EGFR 2/ErbB2; TAK 165, 366017-09-6; Tocris Bioscience), formyl peptide receptor-like 1(FPRL1 ...
-
No products found
because this supplier's products are not listed.
Quote:
... SiRNAs against Nipbl (Nipbl-1: 5’-GTGGTCGTTACCGAAACCGAA-3’; Nipbl-2: 5’-AAGGCAGTACTTAGACTTTAA-3’) and Rad21 (5’-CTCGAGAATGGTAATTGTATA-3’) were made by Qiagen. AllStars Negative Control siRNA was obtained from Qiagen.
-
No products found
because this supplier's products are not listed.
Defne Bayik, et al.,
bioRxiv - Pathology 2019
Quote:
... and blocked in FACS buffer (PBS, 2% BSA) containing an FcR blocking reagent (Miltenyi Biotec). Samples were stained with fluorophore-conjugated antibody cocktails ...
-
No products found
because this supplier's products are not listed.
Laura A. Baker, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... Peptides were separated using a 15 cm chip column (ChromXP C18, 3 μm, 120 Å)(SCIEX). The mass spectrometer was operated in positive ion mode using a data dependent acquisition method (DDA ...
-
No products found
because this supplier's products are not listed.
Eric T. Son, et al.,
bioRxiv - Immunology 2020
Quote:
... Blocking with mouse Fc Block (BD Biosciences, catalogue# 553141) was performed for 10 minutes at 4°C and either or both of mouse anti-PE (clone PE001 ...
-
No products found
because this supplier's products are not listed.
Quote:
... The production of titan cells was also assessed in the presence of various reagents added at step 3: (1) Qsp1 peptide (NFGAPGGAYPW, [33]) (Biomatik, Cambridge, Canada) was resuspended at 10mM in water and stored at −80°C until use at 10 µM final with the scrambled peptide (AYAPWFGNPG ...
-
No products found
because this supplier's products are not listed.
Quote:
... Tissue sections were incubated with the rabbit polyclonal active anti-Caspase-3 primary antibody (directed against a peptide from the P17 fragment of the activated human/mouse Caspase-3) (R&D system, USA). This primary incubation lasted 2 hours at 4°C ...
-
No products found
because this supplier's products are not listed.
Jenny Clarke, et al.,
bioRxiv - Microbiology 2019
Quote:
... After blocking (Peprotech), samples were added to the wells and incubated for 2 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Odon Thiébeauld, et al.,
bioRxiv - Plant Biology 2017
Quote:
Biotinylated peptides (synthetized by Eurogentec, sequences shown in Figure 3) were quite insoluble in water and were resuspended in 6M urea ...
-
No products found
because this supplier's products are not listed.
Patpicha Arunsan, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... The peptide rOv-GRN-1was concentrated using Centripep with cut-off 3 kDa (Eppendorf) and resuspended in low salt solution ...
-
No products found
because this supplier's products are not listed.
Quote:
... we used microscopy system 3 (Leica DMi8, Table 2).
-
No products found
because this supplier's products are not listed.
Thomas O’Loughlin, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2’,3’-cGAMP (Invivogen) at 10 μg/mL ...
-
No products found
because this supplier's products are not listed.
M. M. Cooley, et al.,
bioRxiv - Pathology 2020
Quote:
... Blocking and incubations were done in PBS supplemented with 3% bovine serum albumin (Calbiochem), 2% goat serum (Sigma) ...
-
No products found
because this supplier's products are not listed.
Quote:
... and blocking with TBS/Blocking buffer (Rockland), the membrane was probed with anti-DmNMNAT (1:8,000 ...
-
No products found
because this supplier's products are not listed.
Gregory R Smith, et al.,
bioRxiv - Systems Biology 2018
Quote:
... and passaged every 2–3 days with 0.25% trypsin (Corning# 25-053-CI) to maintain subconfluency ...
-
No products found
because this supplier's products are not listed.
Quote:
... Novaplus) followed by freshly prepared 2% glutaraldehyde/2% paraformaldehyde in 0.1M PB solution filtered with #3 filter paper (VWR) and pH to 7.4 ...
-
No products found
because this supplier's products are not listed.
Quote:
Dam1C/DASH-MT complexes (3 µl) were applied on the carbon side of a Quantifoil 2/2 grid (Quantifoil Micro Tools GmbH ...
-
No products found
because this supplier's products are not listed.
Jérémie Le Pen, et al.,
bioRxiv - Immunology 2017
Quote:
... 2 μg of RNA were submitted to 3′ ligation using the TruSeq Small RNA kit (Illumina), following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jenny L. M. Digby, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... MEIS1/2/3 (Active Motif 39796) and LTL (Vectorshield FL-1321) ...
-
No products found
because this supplier's products are not listed.
Quote:
... 2-to-3-month-old C57BL/6J female mice (Jackson Laboratory) were used for fluorescent-minus-one (FMO ...
-
No products found
because this supplier's products are not listed.
Quote:
CC mice received 2 mg of IFNAR-blocking mouse mAb (MAR1-5A3, BioXCell) by intraperitoneal (IP ...
-
No products found
because this supplier's products are not listed.
Quote:
... 2-(4-(3-(4-acetyl-3-hydroxy-2-propylphenoxy) propoxy) phenoxy acetic acid (PHE) was purchased from Cayman Chemical (Ann Arbor, MI, USA). 10-fold concentrated phosphate buffer (PBS ...
-
No products found
because this supplier's products are not listed.
Quote:
... agatoxin (Peptides International), and calmodulin (Sigma-Aldrich).
-
No products found
because this supplier's products are not listed.
Quote:
... pure resin at 30°C for 2 × 12 h and pure resin with 3% accelerator (BDMA, EMS) at 30°C for 12 h ...