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No products found
because this supplier's products are not listed.
Monica Varela, et al.,
bioRxiv - Immunology 2019
Quote:
Impermeable Sytox Green Nucleic Acid Stain (ThermoFisher Scientific) was used following manufacturer instructions at a final concentration of 5 μM to quantify cell death in RAW264.7 macrophages.
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No products found
because this supplier's products are not listed.
Jonathan J. Wilde, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... post-stained with GelRed Nucleic Acid Gel Stain (Biotium) according to manufacturer’s instructions and visualized using an InGenius Gel Documentation System (Syngene).
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No products found
because this supplier's products are not listed.
Havell Markus, et al.,
bioRxiv - Genomics 2019
Quote:
... DNA was extracted using QIAamp Circulating Nucleic Acid kit (QIAGEN). We measured DNA yield using digital PCR assay22 ...
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No products found
because this supplier's products are not listed.
Borja Garnelo Gomez, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The SYBRR Green I nucleic acid gel stain (Sigma-Aldrich) was used for amplification ...
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No products found
because this supplier's products are not listed.
Bryan P. Brown, et al.,
bioRxiv - Microbiology 2019
Quote:
Viral nucleic acid was extracted from 200µl of the filtrate using the High Pure viral nucleic acid kit (Roche Diagnostics, USA) following the standard protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... samples were incubated with Diamond™ Nucleic Acid Dye (Promega, Madison, WI), which can cross cell membranes ...
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No products found
because this supplier's products are not listed.
Quote:
The 10,000×SYBR Green 1 (SG1) nucleic acid stain (Lonza America, Alpharetta, GA, USA) was purchased and stored at −30ºC ...
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No products found
because this supplier's products are not listed.
Daan C. Swarts, Martin Jinek,
bioRxiv - Biochemistry 2018
Quote:
... was added to the sample and the DNA was resolved on 0.8% agarose gels stained with GelRed Nucleic Acid Gel Stain (Biotum) and visualized using a ChemiDoc Touch gel imager (Bio-Rad).
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No products found
because this supplier's products are not listed.
Quote:
... Nucleic acid quality and quantity were determined by Agilent Tapestation and Qubit Fluorometry respectively.
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No products found
because this supplier's products are not listed.
Quote:
... Green fluorescent nucleic acid stain signal was measured with FITC filter set on the Envision 2103 multilabel microplate reader (Perkin Elmer). The background fluorescence of medium-only with CyQuant reagent was subtracted for data normalization.
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No products found
because this supplier's products are not listed.
U-Ging Lo, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... the 15% TBE-Urea gel was then stained with GelRed− Nucleic Acid Gel Stains (VWR) and visualized under UV light in the Alpha Imager devise (Protein Simple) ...
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acid was treated with DNAseI (NEB, Catalogue number M0303L) and 10 replicates per genotype were processed ...
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No products found
because this supplier's products are not listed.
Tobias Vornholt, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... nucleic acids were digested by addition of 1 µL benzonase (Merck KGaA) and 10 µL 1 M MgSO4 followed by incubation at room temperature for 10 min ...
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No products found
because this supplier's products are not listed.
Franklin L. Zhong, et al.,
bioRxiv - Immunology 2018
Quote:
... a surface stain was performed for 20 minutes in Brilliant Stain Buffer (BD Biosciences) containing the following antibodies ...
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No products found
because this supplier's products are not listed.
Quote:
... transfer of nucleic acids to a Nytran supercharge nylon membrane (GE Healthcare Life Sciences) using a semidry transfer apparatus (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Mohd Ezhar Mohd Noor, et al.,
bioRxiv - Microbiology 2017
Quote:
Nucleic acid extractions were performed by using Metagenome DNA isolation kit (Epicentre) and PowerWater DNA Isolation Kit (MO BIO Lab ...
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No products found
because this supplier's products are not listed.
Xiao Xuan Huang, et al.,
bioRxiv - Microbiology 2018
Quote:
... The peptide nucleic acid probe was conjugated to AlexaFluor 488 (supplied by PNA Bio Company ...
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No products found
because this supplier's products are not listed.
Quote:
... Purified RNA was then 5’ end labelled with biotin-maleimide using a 5’ EndTag nucleic acid labelling system (Vector laboratories). Streptavidin coated Dynabeads M-280 (Invitrogen ...
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No products found
because this supplier's products are not listed.
Sergei Perov, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Coomassie Stain (Expedeon - InstantBlue Coomassie Protein Stain) was performed to visualize the migration of soluble CsgA in the gel ...
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No products found
because this supplier's products are not listed.
Sanguk Yun, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and Picrosirius Red stain (Abcam) were used ...
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No products found
because this supplier's products are not listed.
Nagham Alouche, et al.,
bioRxiv - Immunology 2019
Quote:
... Aqua or red Dead Cell Stain Kits (Biolegend) were used.
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No products found
because this supplier's products are not listed.
Quote:
... and free fatty acids (Free Fatty Acid Fluorometric Assay Kit, Cayman Chemical). All assays were performed according to the kit instructions ...
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No products found
because this supplier's products are not listed.
Quote:
Jasmonic acid-d0 and jasmonic acid-d5 were obtained from Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Quote:
... MEM nonessential amino acids (Corning) and antibiotics/antimycotics (Corning) ...
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No products found
because this supplier's products are not listed.
Hannah Laeverenz Schlogelhofer, et al.,
bioRxiv - Microbiology 2019
Quote:
... with nucleic acid staining and confocal microscopy (Olympus Fluoview FV1200) used to confirm an even distribution of cells on the filter ...
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No products found
because this supplier's products are not listed.
Quote:
... and RNA concentration was measured by nucleic acid quantification in Epoch Microplate Spectrophotometer (BioTek, Winooski, VT). Then ...
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No products found
because this supplier's products are not listed.
Quote:
... Phalloidin red (actin-stain™ 647 phalloidin) and green (actin-stain™ 488 phalloidin) were from Cytoskeleton. GM6001 was from Millipore.
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No products found
because this supplier's products are not listed.
C. Coulson-Gilmer, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... REVERT total protein stain solution (LI-COR) was used for normalisation ...
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No products found
because this supplier's products are not listed.
Quote:
... and then crocein scarlet-acid fuchsin solution for 2 minutes (4 parts crocein scarlet – 0.1% crocein scarlet [Alfa Aesar, J66876] and 0.5% glacial acetic acid; 1 part acid fuchsin – 0.1% acid fuchsin [Alfa Aesar, B22222] and 0.5% glacial acetic acid). Sections were then treated 2 times with 5% phosphotungstic acid for 5 minutes each (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Matthew Wind, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... all trans retinoic acid (0.1 µM, Tocris), BDNF (20ng/ml ...
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No products found
because this supplier's products are not listed.
Quote:
... gel containing 1× RedSafe Nucleic Acid Staining Solution (FroggaBio) and electrophoresed in 1× Tris-Acetate-EDTA (TAE ...
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No products found
because this supplier's products are not listed.
Andrew Lugowski, et al.,
bioRxiv - Molecular Biology 2017
Quote:
... were washed with 100 μL of nucleic acid equilibration buffer (Miltenyi Biotec) and then five times with 100 μL of 1x wash buffer ...
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No products found
because this supplier's products are not listed.
Jiatao Li, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... Nucleic acid was cleaned up after each steps using SPRIselect beads (Beckman Coulter). Libraries were then quantified by Qubit and realtime quantitative PCR on a LightCycler 96 System (Roche).
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acids were extracted using the Nucleospin Plant II kit (Macherey-Nagel, Düren, DE) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Lorenzo Calviello, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Precipitated nucleic acids were further purified and concentrated with Zymo-Spin IIC column (Zymo Research). Obtained RNA was depleted of rRNAs with Ribo-Zero Gold Kit (Human/Mouse/Rat ...
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No products found
because this supplier's products are not listed.
José Gustavo Ramírez-Paredes, et al.,
bioRxiv - Microbiology 2019
Quote:
... The extracted nucleic acids were tested using the commercial kit Path-ISKNV-EASY (Primerdesign, Southampton, UK) in the platform Genesig q16® (Primerdesign ...
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No products found
because this supplier's products are not listed.
Quote:
... were purchased from the Stanford Protein and Nucleic Acid facility and molecular cloning was performed using In-Fusion cloning (Clontech). GFP and mDHFR were PCR amplified from pARL2-SBP1-mDHFR-GFP (27 ...
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No products found
because this supplier's products are not listed.
Quote:
The nucleic acid sequences of N-terminal adhesin domains of CfaE (GenBank M55661) was cloned into a pMAL-C5X vector (Addgene) in-frame with a MBP tag to express as periplasmic proteins with improved solubility (MBP-CfaE-N).
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acid was extracted following the standardized Metahit protocol36 (http://www.metahit.eu/ and using the FastPrep instrument (MP Biomedicals, USA). Libraries were prepared following the instructions of “Kapa Library Preparation Kit for Illumina platforms” (Kapabiosystems ...
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No products found
because this supplier's products are not listed.
Claudia Cocozza, et al.,
bioRxiv - Physiology 2018
Quote:
... stained with UranylessEm Stain (Electron Microscopy Science) and lead citrate ...
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No products found
because this supplier's products are not listed.
Quote:
... and stained with Multiple Stain Solution (Polysciences, Inc). Eyes to be processed for immunohistochemistry and retinal flat mounts were harvested and processed in 4% PFA for two hours at room temperature prior to storage in 1M PBS (phosphate buffered saline) ...
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No products found
because this supplier's products are not listed.
Quote:
... stain quantification of total tumor area (necrotic and stromal areas removed) and percent positive stain or stain intensity was performed using ImageScope Aperio Analysis software (Leica, Buffalo Grove, IL). Areas for quantification were annotated using Aperio analysis tools and percent weak ...
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No products found
because this supplier's products are not listed.
Quote:
... Okadaic acid (OA, Calbiochem) and SRPIN340 (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... For some experiments the nuclear stain Draq5 (Cell Signaling #4084S) was used instead of an anti-histone antibody.
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No products found
because this supplier's products are not listed.
Quote:
... Samples for amino acid quantification were spiked with 1 μl of uniformly labeled amino acids (Cambridge Isotope Labs) and derivatized using AccQ-Tag reagent (Waters Corp. ...
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No products found
because this supplier's products are not listed.
Quote:
... Nucleic acid was precipitated by adding 50 μl Sodium Acetate (3M, pH 5.2), an equal volume of isopropanol ...
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No products found
because this supplier's products are not listed.
Elisa Manieri, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Total bile acids were measured using Hall’s Bile Stain Kit (American MasterTech). Bile acid species were examined by a modification of the method described by (Ye ...
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No products found
because this supplier's products are not listed.
L. S. Tran, et al.,
bioRxiv - Immunology 2019
Quote:
... Tissues were stained with Mayer’s Haematoxylin & Eosin or Periodic acid-Schiff (PAS)-Alcian Blue (AB) stains and imaged at 20 x magnification using a bright-field microscope (Nikon). Inflammatory scores for neutrophils and lymphocytes were graded in a blinded fashion (JSP ...
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No products found
because this supplier's products are not listed.
Quote:
Amino acids analysis was conducted on LC/MS/MS system according to EZ:faast protocol for free amino acids (Phenomenex, Torrance, CA, USA). The EZ:faast kit contains standard solutions ...
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No products found
because this supplier's products are not listed.
Quote:
... phosphatidylethanolamine (EYPE) and phosphatidic acid (EYPA) (Avanti Polar Lipids, Alabaster, AL); reagents used in the coupled enzyme assay (Sigma-Aldrich ...