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No products found
because this supplier's products are not listed.
Quote:
... Nucleic acids were stained with SYBR Gold (Invitrogen) and visualized using Typhoon FLA 9500.
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No products found
because this supplier's products are not listed.
Havell Markus, et al.,
bioRxiv - Genomics 2019
Quote:
... DNA was extracted using QIAamp Circulating Nucleic Acid kit (QIAGEN). We measured DNA yield using digital PCR assay22 ...
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No products found
because this supplier's products are not listed.
Bryan P. Brown, et al.,
bioRxiv - Microbiology 2019
Quote:
Viral nucleic acid was extracted from 200µl of the filtrate using the High Pure viral nucleic acid kit (Roche Diagnostics, USA) following the standard protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... with GelRed Nucleic Acid GelStain (1:10,000) (Biotium) at 150V for 45 min ...
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No products found
because this supplier's products are not listed.
Quote:
... samples were incubated with Diamond™ Nucleic Acid Dye (Promega, Madison, WI), which can cross cell membranes ...
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No products found
because this supplier's products are not listed.
Borja Garnelo Gomez, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The SYBRR Green I nucleic acid gel stain (Sigma-Aldrich) was used for amplification ...
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No products found
because this supplier's products are not listed.
Quote:
... Nucleic acid quality and quantity were determined by Agilent Tapestation and Qubit Fluorometry respectively.
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acid was treated with DNAseI (NEB, Catalogue number M0303L) and 10 replicates per genotype were processed ...
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No products found
because this supplier's products are not listed.
Quote:
... and Chemagic MSM1 nucleic acid extraction robot (PerkinElmer).
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No products found
because this supplier's products are not listed.
Quote:
... transfer of nucleic acids to a Nytran supercharge nylon membrane (GE Healthcare Life Sciences) using a semidry transfer apparatus (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Tobias Vornholt, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... nucleic acids were digested by addition of 1 µL benzonase (Merck KGaA) and 10 µL 1 M MgSO4 followed by incubation at room temperature for 10 min ...
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No products found
because this supplier's products are not listed.
Quote:
... and nucleic acids integrity was checked using an automated gel electrophoresis system (Experion, Bio-Rad, Hercules, USA).
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No products found
because this supplier's products are not listed.
Quote:
The 10,000×SYBR Green 1 (SG1) nucleic acid stain (Lonza America, Alpharetta, GA, USA) was purchased and stored at −30ºC ...
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No products found
because this supplier's products are not listed.
Xiao Xuan Huang, et al.,
bioRxiv - Microbiology 2018
Quote:
... The peptide nucleic acid probe was conjugated to AlexaFluor 488 (supplied by PNA Bio Company ...
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No products found
because this supplier's products are not listed.
U-Ging Lo, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... the 15% TBE-Urea gel was then stained with GelRed− Nucleic Acid Gel Stains (VWR) and visualized under UV light in the Alpha Imager devise (Protein Simple) ...
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No products found
because this supplier's products are not listed.
Josephine Axis, et al.,
bioRxiv - Physiology 2018
Quote:
... α-Modification Minimal Essential Medium was obtained from Corning-CellGro ...
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No products found
because this supplier's products are not listed.
Lorenzo Calviello, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Precipitated nucleic acids were further purified and concentrated with Zymo-Spin IIC column (Zymo Research). Obtained RNA was depleted of rRNAs with Ribo-Zero Gold Kit (Human/Mouse/Rat ...
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No products found
because this supplier's products are not listed.
Quote:
... and free fatty acids (Free Fatty Acid Fluorometric Assay Kit, Cayman Chemical). All assays were performed according to the kit instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... The H4 K16ac modification was detected using a monoclonal antibody (Abcam, ab109463) and lysine crotonylation was detected using a pan-specific anti-CrK antibody (PTM Biolabs ...
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No products found
because this supplier's products are not listed.
Mohd Ezhar Mohd Noor, et al.,
bioRxiv - Microbiology 2017
Quote:
Nucleic acid extractions were performed by using Metagenome DNA isolation kit (Epicentre) and PowerWater DNA Isolation Kit (MO BIO Lab ...
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No products found
because this supplier's products are not listed.
Quote:
... and selected on LB (Miller’s modification, Alfa Aesar, USA) plates ...
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No products found
because this supplier's products are not listed.
Quote:
Jasmonic acid-d0 and jasmonic acid-d5 were obtained from Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Jiatao Li, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... Nucleic acid was cleaned up after each steps using SPRIselect beads (Beckman Coulter). Libraries were then quantified by Qubit and realtime quantitative PCR on a LightCycler 96 System (Roche).
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No products found
because this supplier's products are not listed.
Quote:
... were purchased from the Stanford Protein and Nucleic Acid facility and molecular cloning was performed using In-Fusion cloning (Clontech). GFP and mDHFR were PCR amplified from pARL2-SBP1-mDHFR-GFP (27 ...
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No products found
because this supplier's products are not listed.
Tamar Tak, et al.,
bioRxiv - Immunology 2019
Quote:
... with several modifications: CD48 BUV395 (BD biosciences 740236) was used instead of CD48 APC-Cy7 ...
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No products found
because this supplier's products are not listed.
Matthew Wind, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... all trans retinoic acid (0.1 µM, Tocris), BDNF (20ng/ml ...
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acids were extracted using the Nucleospin Plant II kit (Macherey-Nagel, Düren, DE) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... or K4me3K9me3 modifications (GenScript) was use as probe ...
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No products found
because this supplier's products are not listed.
Quote:
The nucleic acid sequences of N-terminal adhesin domains of CfaE (GenBank M55661) was cloned into a pMAL-C5X vector (Addgene) in-frame with a MBP tag to express as periplasmic proteins with improved solubility (MBP-CfaE-N).
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No products found
because this supplier's products are not listed.
Hannah Laeverenz Schlogelhofer, et al.,
bioRxiv - Microbiology 2019
Quote:
... with nucleic acid staining and confocal microscopy (Olympus Fluoview FV1200) used to confirm an even distribution of cells on the filter ...
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No products found
because this supplier's products are not listed.
Quote:
... and RNA concentration was measured by nucleic acid quantification in Epoch Microplate Spectrophotometer (BioTek, Winooski, VT). Then ...
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No products found
because this supplier's products are not listed.
Stephan B. Dreyer, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... according to the manufacturer’s protocols with some modifications (Illumina, Part no ...
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No products found
because this supplier's products are not listed.
Quote:
... Okadaic acid (OA, Calbiochem) and SRPIN340 (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... Samples for amino acid quantification were spiked with 1 μl of uniformly labeled amino acids (Cambridge Isotope Labs) and derivatized using AccQ-Tag reagent (Waters Corp. ...
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No products found
because this supplier's products are not listed.
Quote:
... gel containing 1× RedSafe Nucleic Acid Staining Solution (FroggaBio) and electrophoresed in 1× Tris-Acetate-EDTA (TAE ...
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No products found
because this supplier's products are not listed.
Andrew Lugowski, et al.,
bioRxiv - Molecular Biology 2017
Quote:
... were washed with 100 μL of nucleic acid equilibration buffer (Miltenyi Biotec) and then five times with 100 μL of 1x wash buffer ...
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No products found
because this supplier's products are not listed.
José Gustavo Ramírez-Paredes, et al.,
bioRxiv - Microbiology 2019
Quote:
... The extracted nucleic acids were tested using the commercial kit Path-ISKNV-EASY (Primerdesign, Southampton, UK) in the platform Genesig q16® (Primerdesign ...
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acid was extracted following the standardized Metahit protocol36 (http://www.metahit.eu/ and using the FastPrep instrument (MP Biomedicals, USA). Libraries were prepared following the instructions of “Kapa Library Preparation Kit for Illumina platforms” (Kapabiosystems ...
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No products found
because this supplier's products are not listed.
Quote:
... Nucleic acid was precipitated by adding 50 μl Sodium Acetate (3M, pH 5.2), an equal volume of isopropanol ...
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No products found
because this supplier's products are not listed.
Jingru Zhang, et al.,
bioRxiv - Epidemiology 2019
Quote:
... Bisulfite modification of 200 ng of genomic DNA was performed using the BisulFlash DNA Modification Kit (EpiGentek, USA) by standard methods ...
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No products found
because this supplier's products are not listed.
Mai Luo, et al.,
bioRxiv - Biochemistry 2020
Quote:
Total peptides from TEV protease digestion for isoTOP-ABPP or tryptic peptides for mapping EN219 site of modification on pure RNF114 were pressure loaded onto 250 mm tubing packed with Aqua C18 reverse phase resin (Phenomenex no. 04A-4299), which was previously equilibrated on an Agilent 600 series high-performance liquid chromatograph using the gradient from 100% buffer A to 100% buffer B over 10 min ...
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No products found
because this supplier's products are not listed.
Wesley A. Wierson, et al.,
bioRxiv - Genetics 2019
Quote:
... Nucleic acid dosages were as follows: 1.5 μg Cas9 mRNA (Trilink Biotechnologies), 1 μg each chemically modified sgRNA (Synthego) ...
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No products found
because this supplier's products are not listed.
Quote:
... extraction was performed in 10 % methanol / 1 % acetic acid with Jasmonic-d5 Acid and Salicylic-d4 Acid (CDN Isotopes) as internal standards ...
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No products found
because this supplier's products are not listed.
Quote:
... Assay protocol is based on Nakamura et al (63) with buffer modifications provided by the recombinant Wnt manufacturer (R&D Systems). A more detailed protocol is available upon request ...
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No products found
because this supplier's products are not listed.
Quote:
... phosphatidylethanolamine (EYPE) and phosphatidic acid (EYPA) (Avanti Polar Lipids, Alabaster, AL); reagents used in the coupled enzyme assay (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... All synthetic nucleic acid reagents were purchased from Integrative DNA Technologies (IDT DNA, Coralville, IA). For knock-in of a single GFP11 sequence ...
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No products found
because this supplier's products are not listed.
Quote:
... with minor modifications - MyTaq (Bioline) was used for PCR amplification of adapter ligated DNA ...
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No products found
because this supplier's products are not listed.
Quote:
... Purified RNA was then 5’ end labelled with biotin-maleimide using a 5’ EndTag nucleic acid labelling system (Vector laboratories). Streptavidin coated Dynabeads M-280 (Invitrogen ...
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No products found
because this supplier's products are not listed.
E. M. Luteran, J. D. Kahn, P. J. Paukstelis,
bioRxiv - Biochemistry 2020
Quote:
... were synthesized on a 1 μmol scale using standard phosphoramidite chemistry on an Expedite 8909 Nucleic Acid Synthesizer (PerSeptive Biosystems, Framingham, MA) with reagents from Glen Research (Sterling, VA). Oligonucleotides were purified by denaturing 20 % (19:1 ...
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No products found
because this supplier's products are not listed.
Quote:
... γ-linolenic acid (GLA) and dihomo-γ-linolenic acid (DGLA) (Nu-Chek Prep) were dissolved in absolute ethanol at a final concentration of 50mM as stock solutions ...