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No products found
because this supplier's products are not listed.
Magdy S. Alabady, Bulak A. Arpat,
bioRxiv - Genomics 2016
Quote:
... The quantity and integrity of the RNA was assessed spectrophotometrically and by agarose gel electrophoresis in 1% agarose/6% formaldehyde/1X MOPS gels stained with SYBR gold nucleic acid gel stain (Molecular Probes) in 1X MOPS buffer following electrophoresis [67] ...
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No products found
because this supplier's products are not listed.
Quote:
... Amplicons were visualized after gel electrophoresis and ultrasensitive GelRed® nucleic acid gel stain (Biotium, Fremont, CA, USA) on a UV-light transilluminator ...
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No products found
because this supplier's products are not listed.
Quote:
... and nucleic acids integrity was checked using an automated gel electrophoresis system (Experion, Bio-Rad, Hercules, USA).
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No products found
because this supplier's products are not listed.
Havell Markus, et al.,
bioRxiv - Genomics 2019
Quote:
... DNA was extracted using QIAamp Circulating Nucleic Acid kit (QIAGEN). We measured DNA yield using digital PCR assay22 ...
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No products found
because this supplier's products are not listed.
Bryan P. Brown, et al.,
bioRxiv - Microbiology 2019
Quote:
Viral nucleic acid was extracted from 200µl of the filtrate using the High Pure viral nucleic acid kit (Roche Diagnostics, USA) following the standard protocol ...
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No products found
because this supplier's products are not listed.
Borja Garnelo Gomez, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The SYBRR Green I nucleic acid gel stain (Sigma-Aldrich) was used for amplification ...
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No products found
because this supplier's products are not listed.
Quote:
... Beads were washed four times with IP buffer and bound nucleic acids were isolated using the Wizard SV gel and PCR clean-up kit (Promega). Collected DNA fragments were quantified using RT-PCR as described above with the following primers ...
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No products found
because this supplier's products are not listed.
Quote:
... Nucleic acid quality and quantity were determined by Agilent Tapestation and Qubit Fluorometry respectively.
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No products found
because this supplier's products are not listed.
Quote:
... Native gel electrophoresis followed by phosphorimaging (Typhoon, GE Healthcare) was used to visualize the formation of RNA-protein complexes.
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No products found
because this supplier's products are not listed.
Nayi Wang, et al.,
bioRxiv - Systems Biology 2018
Quote:
... the gel was stained in 15 ml water containing 1.5 μl GelStar Nucleic Acid Gel Stain (Lonza #50535) for 15 minutes ...
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acid was treated with DNAseI (NEB, Catalogue number M0303L) and 10 replicates per genotype were processed ...
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No products found
because this supplier's products are not listed.
Quote:
... purified in gel electrophoresis with Zymoclean Gel DNA Recovery Kit (Zymo Research, D4002), quantified with the nanophotometer (Implen ...
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No products found
because this supplier's products are not listed.
U-Ging Lo, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... the 15% TBE-Urea gel was then stained with GelRed− Nucleic Acid Gel Stains (VWR) and visualized under UV light in the Alpha Imager devise (Protein Simple) ...
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No products found
because this supplier's products are not listed.
Quote:
... and Chemagic MSM1 nucleic acid extraction robot (PerkinElmer).
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No products found
because this supplier's products are not listed.
Yu Sun, et al.,
bioRxiv - Microbiology 2019
Quote:
... agarose gel electrophoresis and Microplate reader (BioTek, FLx800). Negative control with only reagent was added to evaluate laboratory microbial contaminations in the agarose gel electrophoresis ...
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No products found
because this supplier's products are not listed.
Tobias Vornholt, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... nucleic acids were digested by addition of 1 µL benzonase (Merck KGaA) and 10 µL 1 M MgSO4 followed by incubation at room temperature for 10 min ...
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acids were extracted using the Nucleospin Plant II kit (Macherey-Nagel, Düren, DE) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... and the integrity of RNA was verified by resolving in 1% agarose gel in Mupid gel electrophoresis (TaKaRa, Japan).
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No products found
because this supplier's products are not listed.
Ricardo C.H. del Rosario, et al.,
bioRxiv - Immunology 2019
Quote:
... and equal amounts of proteins were resolved by electrophoresis on 12% Tris-HCl gels (Mini-PROTEAN TGX gels; Bio-Rad, #4561043) and transferred onto polyvinylidene difluoride membranes (Trans-Blot Turbo Transfer Pack ...
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No products found
because this supplier's products are not listed.
Vipin Shankar Chelakkot, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... PCR products were detected by agarose gel electrophoresis using RedSafe™ Nucleic Acid Staining Solution (iNtRON Biotechnology, Inc.).
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No products found
because this supplier's products are not listed.
Jiatao Li, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... Nucleic acid was cleaned up after each steps using SPRIselect beads (Beckman Coulter). Libraries were then quantified by Qubit and realtime quantitative PCR on a LightCycler 96 System (Roche).
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No products found
because this supplier's products are not listed.
Quote:
... gel containing 1× RedSafe Nucleic Acid Staining Solution (FroggaBio) and electrophoresed in 1× Tris-Acetate-EDTA (TAE ...
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No products found
because this supplier's products are not listed.
Mohd Ezhar Mohd Noor, et al.,
bioRxiv - Microbiology 2017
Quote:
Nucleic acid extractions were performed by using Metagenome DNA isolation kit (Epicentre) and PowerWater DNA Isolation Kit (MO BIO Lab ...
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No products found
because this supplier's products are not listed.
Xiao Xuan Huang, et al.,
bioRxiv - Microbiology 2018
Quote:
... The peptide nucleic acid probe was conjugated to AlexaFluor 488 (supplied by PNA Bio Company ...
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No products found
because this supplier's products are not listed.
Sara Elfarrash, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Equal volumes of supernatants and pellets were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis on 8-16% Bis-Tris gels (Genscript) that subsequently were stained with Coomassie blue R-250 (Supplementary fig ...
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No products found
because this supplier's products are not listed.
Z Han, et al.,
bioRxiv - Genetics 2019
Quote:
... proteins were fractionated using a Gel Elution Liquid Fraction Entrapment Electrophoresis (GelFree) system (Expedeon) following manufacturer’s specifications and the different fractions were monitored for the presence of Rpb1 by SDS-PAGE ...
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No products found
because this supplier's products are not listed.
Quote:
... of each sample was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting with antibodies against CD63 (LSBio) and TSG-6 (sc-30140; Santa Cruz Biotechnology). This experiment was repeated a minimum of three times to confirm reproducibility.
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No products found
because this supplier's products are not listed.
Quote:
... and free fatty acids (Free Fatty Acid Fluorometric Assay Kit, Cayman Chemical). All assays were performed according to the kit instructions ...
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No products found
because this supplier's products are not listed.
Daniel Delgado-Bellido, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... gel electrophoresis and transferred to PVDF membrane (Pall laboratory) by semi-wet blotting.
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No products found
because this supplier's products are not listed.
Quote:
... PCR products underwent agarose gel electrophoresis and gel purification (Universal DNA purification kit, Tiangen, Beijing, China). For animal experiments ...
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No products found
because this supplier's products are not listed.
Diana I. Martínez-Tobón, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... and verified by DNA gel electrophoresis and sequencing (ABI 3730 DNA sequencer, Applied Biosystems). Constructs were inserted in the expression strain E ...
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No products found
because this supplier's products are not listed.
Nathan D. Elrod, et al.,
bioRxiv - Genomics 2019
Quote:
... Small RNAs were separated by 8% denaturing polyacrylamide gel electrophoresis (National Diagnostics EC-833) and electroblotted/UV crosslinked to Hybond N+ membrane (GE Healthcare RPN303B) ...
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No products found
because this supplier's products are not listed.
Quote:
... Kainic acid (Abcam ab144490) was diluted from 10 mM/ml to 300µM/ml in primary cultured media and added to motor neurons for 10 minutes followed by a replacement with glia-conditioned media for one hour ...
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No products found
because this supplier's products are not listed.
Quote:
... MEM nonessential amino acids (Corning) and antibiotics/antimycotics (Corning) ...
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No products found
because this supplier's products are not listed.
Matthew Wind, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... all trans retinoic acid (0.1 µM, Tocris), BDNF (20ng/ml ...
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No products found
because this supplier's products are not listed.
Hannah Laeverenz Schlogelhofer, et al.,
bioRxiv - Microbiology 2019
Quote:
... with nucleic acid staining and confocal microscopy (Olympus Fluoview FV1200) used to confirm an even distribution of cells on the filter ...
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No products found
because this supplier's products are not listed.
Tao Fan, et al.,
bioRxiv - Cell Biology 2018
Quote:
... Fifty micrograms of protein was subjected to SDS-polyacrylamide gel electrophoresis (12%PAGE; Amresco) and transferred to a polyvinylidene fluoride membrane (Millipore ...
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No products found
because this supplier's products are not listed.
Elliot Zwelibanzi Jiyane, et al.,
bioRxiv - Microbiology 2019
Quote:
Electrophoresis was performed on all samples using 2,0% agarose gel (Crystal TBE, Bioline, UK) for 40 minutes at 100 V ...
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No products found
because this supplier's products are not listed.
Quote:
... Nucleic acids were extracted using either Trizol solution or the Mag-Bind Viral DNA/RNA kit (Omega Bio-tek, USA) and eluted into 50μL of water ...
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No products found
because this supplier's products are not listed.
Quote:
... Total nucleic acid was extracted following the standardized Metahit protocol36 (http://www.metahit.eu/ and using the FastPrep instrument (MP Biomedicals, USA). Libraries were prepared following the instructions of “Kapa Library Preparation Kit for Illumina platforms” (Kapabiosystems ...
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No products found
because this supplier's products are not listed.
Quote:
... Products were resolved in agarose gel electrophoresis and imaged using Odyssey Scanner (LI-COR Biosciences, Lincoln, NE).
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No products found
because this supplier's products are not listed.
Quote:
... and then crocein scarlet-acid fuchsin solution for 2 minutes (4 parts crocein scarlet – 0.1% crocein scarlet [Alfa Aesar, J66876] and 0.5% glacial acetic acid; 1 part acid fuchsin – 0.1% acid fuchsin [Alfa Aesar, B22222] and 0.5% glacial acetic acid). Sections were then treated 2 times with 5% phosphotungstic acid for 5 minutes each (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... Okadaic acid (OA, Calbiochem) and SRPIN340 (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... 20 ml casamino acids (BD) (10 % (m/V)) ...
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No products found
because this supplier's products are not listed.
Andrew Lugowski, et al.,
bioRxiv - Molecular Biology 2017
Quote:
... were washed with 100 μL of nucleic acid equilibration buffer (Miltenyi Biotec) and then five times with 100 μL of 1x wash buffer ...
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No products found
because this supplier's products are not listed.
Quote:
... Electrophoresis gels were made with 1% agarose (Eurogentec, Seraing, Belgium) in tris-acetate-EDTA (TAE ...
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No products found
because this supplier's products are not listed.
Quote:
The nucleic acid sequences of N-terminal adhesin domains of CfaE (GenBank M55661) was cloned into a pMAL-C5X vector (Addgene) in-frame with a MBP tag to express as periplasmic proteins with improved solubility (MBP-CfaE-N).
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No products found
because this supplier's products are not listed.
Shira Weingarten-Gabbay, et al.,
bioRxiv - Genomics 2017
Quote:
... Digested DNA was separated from smaller fragments and uncut PCR products by electrophoresis on a 2.5% agarose gel stained with GelStar (Cambrex Bio Science Rockland). Fragments in the size of 200bp were cut from the gel and eluted using electroelution Midi GeBAflex tubes (GeBA ...
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No products found
because this supplier's products are not listed.
Cara R. Schiavon, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and 1% tannic acid (EMS) for 1h each ...
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No products found
because this supplier's products are not listed.
Quote:
... Samples for amino acid quantification were spiked with 1 μl of uniformly labeled amino acids (Cambridge Isotope Labs) and derivatized using AccQ-Tag reagent (Waters Corp. ...