-
No products found
because this supplier's products are not listed.
Shixiang Guo, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... precursor and mature miR-15a/miR-16 specific primers were purchased from Thermo Fisher. cDNA synthesis was performed by the High Capacity cDNA Synthesis Kit (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Dayaneth Jácome, et al.,
bioRxiv - Neuroscience 2023
Quote:
... LNA™ PCR primer set (Qiagen). PCR amplification and detection were performed with the Applied Biosystems StepOnePlus Real-Time detector ...
-
No products found
because this supplier's products are not listed.
Mariska Miranda, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Standard RT-PCR using independent sets of Predesigned KiCqStart® SYBR® Green Primers (Sigma, Table S11) was carried out for validation with independent biological replicates from 2D cultures (n = 2 ...
-
No products found
because this supplier's products are not listed.
Amit Prabhakar, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... qRT-PCR reactions were performed using pre-miR-primers by real-time PCR machine (CFX96, BioRad). 100 U/ml RNase inhibitor (SUPERase•in™ ...
-
No products found
because this supplier's products are not listed.
Nikita Vasilyev, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Index Primers Set 3 (NEB). Amplified libraries were cleaned up using Sample Purification Beads (NEB ...
-
No products found
because this supplier's products are not listed.
Yasuo Ariumi,
bioRxiv - Microbiology 2021
Quote:
... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ...
-
No products found
because this supplier's products are not listed.
Yi-Chen Chen, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The DNA fragments around the crRNA target site were amplified by a two-step PCR with specific primer sets (Table S2) and Index PCR Primers mentioned in the manufacturer’s instructions (Illumina, USA). After gel purification ...
-
No products found
because this supplier's products are not listed.
Anna Tejchman-Skrzyszewska, et al.,
bioRxiv - Neuroscience 2023
Quote:
... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega ...
-
No products found
because this supplier's products are not listed.
Kasper Mikkelsen, et al.,
bioRxiv - Microbiology 2023
Quote:
... RT-PCR reactions were set up using the FastStart Essential DNA Green Master kit (Roche), using four different primer pairs ...
-
No products found
because this supplier's products are not listed.
Bonita H. Powell, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... TaqMan miRNA stem-loop RT primers/qPCR primer probe sets (ABI 4427975) (cel-miR-39 ID# 000200 Lot#P180110-003B10 ...
-
No products found
because this supplier's products are not listed.
Baojun Ren, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Mycoplasma Plus PCR Primer Set (Agilent) was used to determine mycoplasma for all cell lines ...
-
No products found
because this supplier's products are not listed.
Michael J. Podolsky, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Quantitative real-time PCR reactions were performed with different sets of primers and Sensifast SYBR (Bioline, Taunton, MA) on a CFX96 or CFX384 Real-Time PCR Detection System (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Syed Moiz Ahmed, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... by overnight capillary transfer at RT using 20X SSC transfer buffer (3M NaCl, 0.3M Sodium citrate, pH 7). DNA was fixed on the membrane by UV-crosslinking at 1.2J/cm2 for 30s in HL-2000 Hybrilinker (UVP ...
-
No products found
because this supplier's products are not listed.
Grant F Marshall, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Primers for reverse-transcription PCR (RT-PCR) and quantitative RT-PCR (qRT-PCR) were supplied by Merck with standard desalting at 100 µM in TE buffer ...
-
No products found
because this supplier's products are not listed.
Noushin Hadadi, et al.,
bioRxiv - Systems Biology 2019
Quote:
... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
-
No products found
because this supplier's products are not listed.
Sean O’Connor, et al.,
bioRxiv - Cell Biology 2020
Quote:
... primary miRNAs for miR-29a and miR-16 were amplified from genomic DNA by PCR and cloned into the pAdTrack shuttle (Addgene, Plasmid#16404). The miR-16 pAdTrack plasmid was then mutated by site-directed mutagenesis to induce 3 point mutations into the miR-16 seed sequence ...
-
No products found
because this supplier's products are not listed.
Kaneez Fatima, et al.,
bioRxiv - Cell Biology 2024
Quote:
... TransIT-X2 (MirusBio, MIR 6000) or TransIT2020 (Mirus, MIR 5400) were used for cell transfection according to manufacturer’s instruction.
-
No products found
because this supplier's products are not listed.
Wei Yu, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Mouse positive control primer set Actb2 (#71017, Active Motif) and mouse negative control primer set 1 (#71011 ...
-
No products found
because this supplier's products are not listed.
Karine Pozo, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Cilia R Pothast, et al.,
bioRxiv - Immunology 2022
Quote:
... Smartseq2modified PCR primer (Eurogentec) and TRAC or TRBC1/2 specific primers (Eurogentec ...
-
No products found
because this supplier's products are not listed.
Leonardo Lupori, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The primer sets used were Quick-16S™ Primer Set V3-V4 (Zymo Research). The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation ...
-
No products found
because this supplier's products are not listed.
Alexandr Samocha, et al.,
bioRxiv - Cell Biology 2020
Quote:
... RT PCR was performed using the RealPlex2 (Eppendorf). Expression was normalized to ß-Actin (Mm02619580_g1 ...
-
No products found
because this supplier's products are not listed.
Yumei Qin, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Genotypes were confirmed using primer sets recommended by Jackson Laboratory and Sinha lab.
-
No products found
because this supplier's products are not listed.
Liam McDonnell, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... SSC-A (PMT voltage = 200), FITC-A (PMT voltage = 450) ...
-
No products found
because this supplier's products are not listed.
Astrid Hoermann, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
-
No products found
because this supplier's products are not listed.
Shubhangini Tiwari, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The cDNA was obtained by RT-PCR and amplified through PCR using respective primers (OriGene Technologies) followed by agarose gel electrophoresis to assess the gene expression ...
-
No products found
because this supplier's products are not listed.
Philip R Nicovich, et al.,
bioRxiv - Neuroscience 2019
Quote:
... RT incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421™ Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ...
-
No products found
because this supplier's products are not listed.
Coralie Dessauges, et al.,
bioRxiv - Systems Biology 2022
Quote:
... The following primers were used for the RT-qPCR reaction (designed with the Real-time PCR (TaqMan) Primer and Probes Design Tool from GenScript).
-
No products found
because this supplier's products are not listed.
Pastor Jullian Fabres, et al.,
bioRxiv - Plant Biology 2021
Quote:
... RT-PCR products were purified using PCR Clean-up (Macherey-Nagel, 740609.250) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Peter F. Renz, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 12 μl Bead RT/PCR Enhancer reagent from BD Biosciences and 72 μl nuclease free water for a total volume of 200 μl ...
-
No products found
because this supplier's products are not listed.
Ekaterina S. Potekhina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... OneTube RT-PCR SYBR kit (Evrogen) was used for cDNA production by reverse transcription followed by qPCR ...
-
No products found
because this supplier's products are not listed.
Katarzyna Goljanek-Whysall, et al.,
bioRxiv - Physiology 2019
Quote:
... with 100nM miR scrambled or miR-181a mimic (100nM; GE Healthcare) (Soriano-Arroquia et al. ...
-
No products found
because this supplier's products are not listed.
Zhihua Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
-
No products found
Sean Manning, et al.,
bioRxiv - Genomics 2021
Quote:
... and PCR amplified using with a 5′-primer for mmu-miR-682 (CTGCAGTCACAGTGAAGTCTG) and the universal 3′ miRNA primer (Applied Biological Materials). Relative Ct values were calculated based on start-point samples and compared to the mid-(only for IL-KO ...
-
No products found
because this supplier's products are not listed.
Connor G. G. Bamford, et al.,
bioRxiv - Microbiology 2021
Quote:
... these were run as two separate multiplex PCR “pools” (A & B) using the ARTIC version 3 primer set (ARTIC nCoV-2019 V3 Panel, IDT DNA Inc ...
-
No products found
because this supplier's products are not listed.
Charlotte Bruzeau, et al.,
bioRxiv - Immunology 2023
Quote:
... REF#S32356) (1/300e in 4X SSC) during 1h at RT and mounted with vectashield containing DAPI (Vector Labs). Images were acquired with an epifluorescence microscope (NIKON) ...
-
No products found
because this supplier's products are not listed.
Krisztina Percze, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 130 of the colonies were analysed by colony PCR (using an M13 primer set, Table S1) and capillary electrophoresis (LabChip GX, PerkinElmer) using the DNA 1K Reagent Kit with DNA HT 5K LabChip single sipper chip ...
-
No products found
because this supplier's products are not listed.
Leelavathi N Madhu, et al.,
bioRxiv - Neuroscience 2024
Quote:
... The qRT-PCR was performed using RT² SYBR Green qPCR Mastermix and Primer mix (GeneCopoeia, Rockville, Maryland, USA) for measuring human microglial gene expression in iMicroglia (tmem119 ...
-
No products found
because this supplier's products are not listed.
Alena Pance, et al.,
bioRxiv - Cell Biology 2021
Quote:
... An optical modification was made on the primary laser detection pod so that the scattered light from 488nm laser light was split into two using a 50/50 beam splitter to measure the normal SSC (vertical) and depolarised SSC (Horizontal) by placing a polarizer (Chroma Technology Corp) with its polarisation axis horizontal to the polarisation plane of the laser light ...
-
No products found
because this supplier's products are not listed.
Peng Wang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and phosphatase inhibitor cocktail set III (Calbiochem). Protein was quantified with Pierce BCA protein assay kit (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Justin X. Boeckman, et al.,
bioRxiv - Pathology 2021
Quote:
... Positive bands of appropriate size were confirmed using individual primer sets and resultant PCR products were visualized on a 1.5% agarose gel with gel red (Biotium).
-
No products found
because this supplier's products are not listed.
Teketel A. Haile, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Two allele specific forward primers and a reverse primer were designed for use in fluorescence based competitive allele-specific PCR assays (KASP; LGC Biosearch Technologies). DNA from the 286 lines was assayed using these primers and KASP reaction mix (LGC Biosearch Technologies ...
-
No products found
because this supplier's products are not listed.
Felix J. Flomm, et al.,
bioRxiv - Microbiology 2022
Quote:
... and corresponding filter sets (Nikon). Imaging conditions were optimized for each sample ...
-
No products found
because this supplier's products are not listed.
Wataru Yamamoto, Rafael Yuste,
bioRxiv - Neuroscience 2019
Quote:
... equipped with a long-pass GFP filter set (Leica filter set ET GFP M205FA/M165FC), 1.63X Plan Apo objective ...
-
No products found
because this supplier's products are not listed.
Takehiro Takahashi, et al.,
bioRxiv - Neuroscience 2021
Quote:
... a commercially available specific primer set was used (System Biosciences). For the detection of some other targets ...
-
No products found
because this supplier's products are not listed.
Tian Huang, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 5x SSC buffer was prepared from 20x SSC buffer (CORNING, 46-020-CM).
-
No products found
because this supplier's products are not listed.
Erick X. Pérez-Guzmán, et al.,
bioRxiv - Microbiology 2019
Quote:
... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Jessica M Toothaker, et al.,
bioRxiv - Immunology 2021
Quote:
... Slides were soaked in SSC/0.1% NP-40 (ab142227, Abcam) to remove cover slips and placed in 2X SSC/0.1% NP-40 for 2 minutes at 74ºC before mounting with antifade plus Propidium Iodide (p36935 ...
-
No products found
because this supplier's products are not listed.
Shivnarayan Dhuppar, Sitara Roy, Aprotim Mazumder,
bioRxiv - Cell Biology 2020
Quote:
... Filter sets from Olympus and Chroma Technology Corp (49309 ET – Orange#2 FISH and 49310 ET – Red#2 FISH ...
-
No products found
because this supplier's products are not listed.
Aleksandr Alekseev, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Filter set 10 (Carl Zeiss) was used for separating the fluorescence and the excitation light ...