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rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit
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Cohesion Biosciences Sponsored
Detect mature miRNA rno-mir-22 expression normalized by U6 snRNA.Cat# CPK2323, 50 rxns USD $300.0, 100 rxns USD $480.0, 200 rxns USD $750.0 Ask
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Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018, published in BMC Immunology doi: 10.1186/s12865-019-0287-yQuote: ... 2 µl was used in real time RT-PCR reactions performed on CFX96 Touch Real-Time PCR Detection System (Bio-Rad) in a 20µl volume with iQ SYBR®Green Supermix (Bio-Rad) ... -
Thermo Fisher
No products found because this supplier's products are not listed.Cited in Novel regulators of nitric oxide signaling triggered by host perception in a plant pathogenbioRxiv - Pathology 2019, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1918977117Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... Real-time RT-PCR was performed using the QuantiTech RT-PCR kit (Qiagen). The primers were designed as previously described and used in a one-step real-time RT-PCR [18] ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Journal of General Virology doi: 10.1099/jgv.0.001231Quote: ... Quantitative real-time RT-PCR was performed using theSYBR PrimeScript RT-kit(TaKaRa Bio) according to the manufacturer’s instructions.The relative transcript levels were analyzed using the ΔΔCt method ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020, published in STEM CELLS doi: 10.1002/stem.3272Quote: ... The RT product was amplified by real-time PCR (Roche LightCycler® 480 PCR instrument) using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™) ... -
American BioInnovations,
No products found because this supplier's products are not listed.Cited in DDX3 is exploited by Arenaviruses to suppress type I interferons and favor their replicationbioRxiv - Microbiology 2017Quote: ... and a Real-Time PCR Detection System (ABI). For BSL-4 infections ... -
Agilent
No products found because this supplier's products are not listed.Cited in Cell type identity determines transcriptomic immune responses in Arabidopsis thaliana rootsbioRxiv - Plant Biology 2018Quote: ... in a Stratagene Mx3005P Real-time PCR Detection System (Agilent Technologies) following a standard protocol ... -
Eppendorf
No products found because this supplier's products are not listed.Cited in 22-hydroxycholesterol leads to efficient dopaminergic specification of human Mesenchymal Stem CellsbioRxiv - Neuroscience 2018Quote: qRT-PCR experiments were performed using Realplex real time PCR detection system (Eppendorf, Germany) as previously described (Singh et al. ... -
Illumina
No products found because this supplier's products are not listed.Cited in Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019, published in iScience doi: 10.1016/j.isci.2020.101345Quote: ... a real-time PCR instrument (Eco Real-Time PCR System, Illumina) was used with the same primers (Table S1 ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018Quote: ... QRT-PCR was conducted with the GoTaq® Real-Time PCR Systems reagent kit (Promega, Beijing, China), and miRcute Plus miRNA Real-Time PCR Systems reagent kit (TianGen ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2016, published in Scientific Reports doi: 10.1038/s41598-017-01133-4Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using the SensiFast SYBR No-ROX Kit (Bioline) on a Rotor-Gene Q machine (Qiagen) ... -
Millipore Sigma
No products found because this supplier's products are not listed.Cited in Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Real-time RT-PCR (TaqMan) assay-specific primers and probes for ZIKV were designed by Sigma-Aldrich (St Louis, MO) following the protocol developed by the Molecular Diagnostics and Research Laboratory Centers for Disease Control and Prevention (CDC) ... -
TianGen Biotech
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018Quote: ... and miRcute Plus miRNA Real-Time PCR Systems reagent kit (TianGen, Beijing, China) following the manufacturer’s instructions ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2019Quote: ... and quantified by qPCR on a Bio-Rad CFX Connect Real-Time PCR detection system with the NEBNext Library Quant Kit for Illumina (New England Biolabs, catalog # E7630L). The libraries were normalized to 10 nM ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2020Quote: ... LMW and HMW Gel Filtration calibration kits (GE Healthcare) were used to estimate the molecular weight of protein complexes ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Genetics 2020Quote: ... The U6:3 promoter was PCR amplified from pAC-U63-tgRNA-Rev (Addgene #112811). The CR7T promoter was synthesized as a gBlock DNA fragment (IDT ... -
Primerdesign
No products found because this supplier's products are not listed.Cited in Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5bioRxiv - Cell Biology 2018, published in Nature Communications doi: 10.1038/s41467-018-07444-yQuote: ... The real-time PCR was subsequently performed on a Bio-Rad CFX96 Touch™ real-time PCR detection system using SYBR green based PrecisionFAST with LOW ROX qPCR kit (Primerdesign). The specificity of PCR primers were verified by both melt curves and agarose gel electrophoresis ... -
VWR
No products found because this supplier's products are not listed.Cited in Fatal neuroinvasion of SARS-CoV-2 in K18-hACE2 mice is partially dependent on hACE2 expressionbioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (Corman et al. ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Genomics 2018, published in Cell doi: 10.1016/j.cell.2018.07.001Quote: ... Cells were regularly cultured for three days in antibiotic-free medium and tested for mycoplasma using either the LookOut Mycoplasma PCR Detection Kit or the MycoAlert mycoplasma detection kit (Lonza). -
BioLegend
No products found because this supplier's products are not listed.Cited in Type 2 innate immunity regulates hair follicle homeostasis to control Demodex pathosymbiontsbioRxiv - Immunology 2021Quote: ... Serum IL-22 was measured using LEGEND MAXTM Mouse IL-22 ELISA Kit (BioLegend, #436307) and analyzed using a SpectraMax M2 microplate reader using SoftMax Pro software (Molecular Devices). -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.Cited in Hormetic Shifting of Redox Environment by Pro-Oxidative Resveratrol Protects Cells Against StressbioRxiv - Biochemistry 2016, published in Free Radical Biology and Medicine doi: 10.1016/j.freeradbiomed.2016.08.006Quote: ... Trypsinised cells were subjected to quantitative real-time PCR and 25 mM GSH measured in the Accuri C6 flow cytometer (BD Biosciences). Data were analysed using GraphPad Prism 5.0 and FlowJo 7.6 (Tree Star). -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019Quote: ... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes. -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in eLife doi: 10.7554/eLife.44574Quote: ... or the Ingenio electroporation kit (Mirus, #MIR 50117) with 1.5 µg of either control siRNA (ON-TARGETplus Non-Targeting Pool ... -
Spherotech
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2021Quote: ... Calibration beads (Spherotech, RCP30-5A) were measured at the end of each cytometry session ... -
Vector Labs
No products found because this supplier's products are not listed.Cited in POT1a depletion in the developing brain leads to p53-dependent neuronal cell death and ataxiabioRxiv - Molecular Biology 2018Quote: ... Detection was performed using the ImmPRESS HRP Polymer Detection Kit (Vector Labs) and the Metal Enhanced DAB Substrate Kit (Thermo Scientific ... -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... Colonization or depletion was verified by real time PCR of fecal DNA (Quick-DNA Fecal/Soil microbe miniprep kit, Zymo research, Cat. No. D6010). PCR was performed in presence of Power SYBR Green PCR master mix (Applied Biosystems ... -
ACEA Biosciences
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... Cell growth and survival were monitored in real time using iCELLigence and xCELLigence real-time cell analysis (RTCA ACEA Biosciences) by seeding 20.000 cells and 5.000 cells respectively ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in Frontiers in Immunology doi: 10.3389/fimmu.2020.00780Quote: ... After purification of the RT-PCR products using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... Real time quantitative PCR was performed with KAPA SYBR FAST qPCR Master Mix (Sigma-Aldrich - Merck, UK, cat no KK4602) according to manufacturer’s instructions in a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Genomics 2018Quote: ... In-situ Apoptosis Detection Kit (Abcam) was used to check for the apoptotic cells in the tissue sections according to the Manufacturer’s protocol ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Pathology 2019Quote: ... Secondary detection was performed this the Bond Polymer Refine Detection Kit (Leica, DS9800). Tissue sections were imaged using a Nikon Eclipse Ci light microscope with 4x or 20x objectives and Nikon Elements software. -
Biotium Inc.
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in mSystems doi: 10.1128/mSystems00588-19Quote: ... The qPCR was performed using EvaGreen Real-time PCR (Biotium, Hayward, USA) according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium ... -
iNtRON Biotechnology
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0198978Quote: ... RT-PCR was performed using the Maxime PCR Premix Kit (Intron Biotechnology, Seoul, Korea). Transcription of HC and LC genes was confirmed with primers as described above ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Genomics 2019, published in Chromosoma doi: 10.1007/s00412-020-00734-9Quote: ... F121-9 mESC were seeded on 0.1% gelatin-coated 22 × 22 mm coverslips (Corning, 2850-22), with total cell number 150-250 × 103 cells/coverslip ... -
Applied Biological Materials
To help researchers in the global fight against the coronavirus, abm has developed an RT-qPCR...Cat# G628, 100 Rxns/kit, please contact supplier for pricing. AskbioRxiv - Cancer Biology 2019Quote: ... and absence of mycoplasma was confirmed using Mycoplasma PCR detection kit (Applied Biological Materials). -
Molecular Devices
No products found because this supplier's products are not listed.Cited in A distinct pool of Nav1.5 channels at the lateral membrane of murine ventricular cardiomyocytesbioRxiv - Physiology 2019, published in Frontiers in Physiology doi: 10.3389/fphys.2019.00834Quote: ... Currents were measured at RT (22–23°C) using a low-noise Axopatch 200B amplifier (Molecular Devices, Sunnyvale, CA, USA). The internal pipette solution was composed of (in mM ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2017, published in eLife doi: 10.7554/elife.32332Quote: ... TSA Plus DIG detection Kit (Perkin Elmer) was used for signal amplification ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Scientific Reports doi: 10.1038/s41598-019-52648-xQuote: ... triplicate PCR reactions obtained with 22 cycles were pooled and purified using Agencourt AMpure XP beads (Beckman Coulter, Singapore). Products from this first step were then sent to the sequencing facility at the Singapore Centre for Life Science and Engineering (SCELSE ... -
GeneCopoeia
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2018Quote: ... The levels of mature miR-197 were determined with All-in-One™ miRNA qRT-PCR Detection Kit (GeneCopoeia, QP015). The levels of pri-miR-197 were detected with TaqMan pri-microRNA assay kit (Applied Biosystems ... -
Bimake
The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically...Cat# B40013, SKU# B40013-200rxns, 200rxns, $127.00 AskbioRxiv - Pathology 2020Quote: ... The cDNA samples were amplified by Real-time quantitative PCR (RT-qPCR) using SYBR Green qPCR Master Mix (Bimake) and 18S was used as a standard reference ... -
Diagenode
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in eLife doi: 10.7554/eLife.49501Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a QuantStudio 6 instrument (Themo Scientific ... -
Bangs Laboratories
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2020Quote: ... calibration curves were produced immediately prior to each experiment using a Quantum-MESF calibration kit (Bangs Labs, USA) of the appropriate fluorochrome according to the manufacturer’s instructions. -
Eurogentec
No products found because this supplier's products are not listed.Cited in Optimal use of statistical methods to validate reference gene stability in longitudinal studiesbioRxiv - Molecular Biology 2019, published in PLOS ONE doi: 10.1371/journal.pone.0219440Quote: ... Quantitative Real time PCR (qPCR) was performed using Takyon ROX SYBR 2X MasterMix (Eurogentec UF-RSMT-B0701) as a fluorescent detection dye ... -
PacBio
No products found because this supplier's products are not listed.bioRxiv - Genomics 2018, published in Genes doi: 10.3390/genes9080393Quote: ... real-time (SMRT) cells at Pacific Biosciences (PacBio). Sequence data were generated using the PacBio RSII instrument with P6v2 polymerase binding and C4 chemistry kits (P6-C4 ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... IL-22 (R&D Systems) and IFNγ (BD Biosciences ... -
Enzo Life Sciences
No products found because this supplier's products are not listed.Cited in Posttranscriptional regulation of intestinal epithelial cell repair by RNA binding protein IMP1bioRxiv - Physiology 2018Quote: CytoID Autophagy Detection Kit (Enzo Life Sciences) was used to stain single cell suspensions of crypt-enriched intestinal epithelium (1:100 in DPBS supplemented with 10% FBS at 37°C for 30 min ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018Quote: ... Reagents used were: Chemiluminescent Western detection kit (Cell Signaling); cycloheximide (CHX ... -
Molecular Cloning Laboratory
No products found because this supplier's products are not listed.Cited in Chromatin Functional States Correlate with HIV Latency Reversal in Infected Primary CD4+ T cellsbioRxiv - Microbiology 2018, published in eLife doi: 10.7554/elife.34655Quote: ... using 2X HoTaq Real Time PCR kit (McLab) and the appropriate primer-probe combinations described in (Calvanese et al. ... -
Advansta Corporation
Sample-loading buffer and protein stain in one Instant results - see protein bands immediately...Cat# K-11053-B30, 3 ml, USD $189.00/ea AskbioRxiv - Plant Biology 2020, published in New Phytologist doi: 10.1111/nph.17283Quote: ... WesternBright ECL Western blotting detection kit (Advansta) and Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare ... -
Nikon
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020, published in eLife doi: 10.7554/eLife.64207Quote: ... the Z-drift was prevented in real time by a built-in focus lock system (Nikon Perfect Focus).