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No products found
because this supplier's products are not listed.
Christine E. Nelson, et al.,
bioRxiv - Immunology 2022
Quote:
... and control sets (Diagnostica Stago Inc, Parsippany, NJ). Heart rate ...
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No products found
because this supplier's products are not listed.
Archana Verma, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Primer synthesis was by Reprocell.
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No products found
because this supplier's products are not listed.
Albéric A. de Lajarte, et al.,
bioRxiv - Biochemistry 2024
Quote:
... adding a T7 promoter sequence at the forward primer (5′ TAATACGACTCACTATAG 3′) using a 2X PCR PreMix (Syd Labs, Cat. MB067-EQ2N), human cDNA (ZYAGEN ...
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No products found
because this supplier's products are not listed.
M Bayne, et al.,
bioRxiv - Physiology 2019
Quote:
... Plasma levels of counter-regulatory hormones and GHRH levels in cell supernatant were determined by ELISA (Crystal Chem Glucagon 81518; Corticosterone 80556; GH Millipore Sigma 3P EZRMGH45K, GHRH Aviva systems biology OKEH03121).
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No products found
because this supplier's products are not listed.
Ugo Tomasello, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... three sections for each brain electroporated at E14.5 with pUB6-TOM and pSilencer-U6-scram (number of brains, n = 4) or pSilencer-U6-miR-137 (number of brains, n = 4) and injected with retrobeads (Lumafluor) at P9 ...
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No products found
because this supplier's products are not listed.
Sandra L. Bixler, et al.,
bioRxiv - Microbiology 2021
Quote:
Total SARS-CoV-2 E gene and subgenomic E gene target copy numbers were determined by real-time RT-PCR using previously described assays (24–25) and a synthetic RNA containing the subgenomic E RNA sequence (Bio-Synthesis, Inc., Lewisville, TX, USA). Extracted nucleic acid was tested in triplicate (5 μL extracted nucleic acid ...
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No products found
because this supplier's products are not listed.
Seth R. Taylor, et al.,
bioRxiv - Neuroscience 2022
Quote:
... P8 or P14 VGluT2/VGAT-Cre;Ai14;miR-218fl/fl and VGluT2/VGAT-Cre;Ai14;miR-218+/+ mice were rapidly decapitated and brains removed and placed in Hibernate A without Ca2+ (Brainbits #HACA500), supplemented with 0.132 M trehalose and synaptic blockers (AP-V 50 µM ...
-
No products found
because this supplier's products are not listed.
Xiaoli Qi, et al.,
bioRxiv - Biochemistry 2023
Quote:
Glutathione probe RT-AM (Kerafast, Cat. No. EBY001)
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No products found
because this supplier's products are not listed.
Ortal Iancu, et al.,
bioRxiv - Bioengineering 2022
Quote:
... using combinations of 4 primers for Vγ and 3 primers for Jγ regions in each reaction (IdentiClone™ TCRG Gene Clonality Assay, Invivoscribe, Inc.). TRG clonality was ran and analyzed on 2% agarose gel ...
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No products found
because this supplier's products are not listed.
Dennis Alejandro Escolástico-Ortiz, et al.,
bioRxiv - Microbiology 2023
Quote:
... the forward primer Ueda19F (GCI WTY TAY GGI AAR GGI GG) and the reverse primer R6 (GCC ATC ATY TCI CCI GA). The oligonucleotide sequences used for amplification included the selected primers and specific sample indexes conceived for Illumina sequencing.
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Magnetofection
Cat# IV-MAG1,
contains 1 extra small cylinder (ø 2 mm), 1 small cylinder (ø 5 mm), 1 cylinder (ø 10 mm) and 1 square (18x18 mm) magnets, USD $69.00/KIT
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Allison Ogren, et al.,
bioRxiv - Genetics 2019
Quote:
... The culture plate was set on top of a plate magnet (Oz Biosciences) for 20 min and returned to the incubator ...
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No products found
because this supplier's products are not listed.
Tamar (Skaist) Mehlman, et al.,
bioRxiv - Biophysics 2022
Quote:
... we used fragments from the Maybridge 1000 fragment library (Maybridge Ro3 core set), the Edelris Keymical fragment library ...
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DMU-212 is a methylated derivative of Resveratrol, with antimitotic, anti-proliferative,...
Cat# E2203, SKU# E2203-5mg,
5mg, $109.00
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Suman Khan, et al.,
bioRxiv - Microbiology 2023
Quote:
... The composition of the screening set was 0.7% Bioactive collections (Selleck Chemicals, USA), 7.6% HitFinder (Maybridge ...
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No products found
because this supplier's products are not listed.
Rebecca E. Poulsen, et al.,
bioRxiv - Neuroscience 2020
Quote:
6dpf larval zebrafish were set in a 2% low melting point agarose (Progen Biosciences), dorsal side up ...
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No products found
Shuangfen Tao, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... We purchased Target sequences for Bcl-2 miRNA 3’-UTR clone and the one with a site mutation at miR-383-binding site from Creative Biogene (Shirley, NY, USA). We seeded MiR-383-modified cells of GC in 24-well plates ...
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No products found
because this supplier's products are not listed.
Matteo Chighizola, et al.,
bioRxiv - Biophysics 2022
Quote:
... for 30 min at RT) glass-bottomed plates (Willco Wells). The fixed cells were characterised using a Bioscope Catalyst AFM from Bruker ...
-
No products found
because this supplier's products are not listed.
Nathan M. Good, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Extracts were prepared as described using an OS Cell Disrupter set at 25,000 psi (Constant Systems Limited ...
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No products found
because this supplier's products are not listed.
Sudhir Kumar, et al.,
bioRxiv - Microbiology 2021
Quote:
... Gametocyte cultures were set up in 6 well plates using O+ human RBCs (Valley Biomedical, VA, US) and O+ human serum (Interstate Blood Bank ...
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No products found
because this supplier's products are not listed.
Danielle L. Schmitt, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... All filter sets were controlled by an external filter-exchanger (Prior Scientific or Ludl Electronic Products, Ltd). Exposure times ranged between 50 to 100 ms ...
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No products found
because this supplier's products are not listed.
Katie E. Blise, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Single marker positivity thresholds were set using FCS Express Image Cytometry RUO (De Novo Software, Glendale, CA) to visually validate protein biomarker expression overlaid on signal extracted images ...
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No products found
Ons Mamai, et al.,
bioRxiv - Cell Biology 2022
Quote:
... After blocking the membrane for 1h at RT with AdvanBlock (Advansta®), and proteins were detected by incubation with relevant antibodies (Supplementary Table S6 ...
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No products found
because this supplier's products are not listed.
Petra Hafker, et al.,
bioRxiv - Physiology 2021
Quote:
... we used a benchtop chiller with an antifreeze bath set to 1°C (MicroCooler II Model 260010, Boekel Scientific ...
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No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Alternating tangential flow was set to 0.11 L/min (5 L/m2/min) using an XCell Lab Controller (Repligen). The desired rate of medium exchange was achieved by controlling the flow rate of the permeate (spent medium ...
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No products found
because this supplier's products are not listed.
Jiling Feng, Yuexun Tang, Wenwei Fu, Hongxi Xu,
bioRxiv - Cell Biology 2023
Quote:
... RT-PCR was performed with a one-step real time PCR using KAPA SYBR FAST One-Step qRT-PCR Universal (D-MARK Biosciences). Primers were used as previously described [18] ...
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No products found
because this supplier's products are not listed.
Katie N. Kang, Joseph M. Boll,
bioRxiv - Microbiology 2022
Quote:
... overnight cultures were back diluted to OD600 0.01 and set up as triplicate biological replicas in either a 96-well plate or 24-well plate (BrandTech BRAND). A BioTek SynergyNeo2 microplate reader was used to record optical density ...
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No products found
because this supplier's products are not listed.
Courtney A. Copeland, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Hypoxia-treated cells were transferred to a tissue culture glovebox set at 0.5% oxygen and 5% CO2 (Coy Lab Products). Medium was supplemented with DMSO 0.1% or BAY (10 μM ...
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No products found
because this supplier's products are not listed.
Kristin L. Rosche, et al.,
bioRxiv - Microbiology 2023
Quote:
... cells were transfected with 0.05µg of each vector from the UPR/ER stress response luciferase reporter vector set (Signosis, LR-3007) and 0.5µl of Lipofectamine 2000 in Opti-MEM I reduced-serum medium (Gibco ...
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No products found
because this supplier's products are not listed.
Jan Zlamal, et al.,
bioRxiv - Immunology 2022
Quote:
... a calcium independent ligand of PS (200nM, 15 min, RT; Haematologic Technologies, Essex Junction, USA).29,30 Sufficient blocking concentrations of Lactadherin were assessed via Calibrated Automated Thrombogram (CAT ...
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No products found
because this supplier's products are not listed.
R. Doolan, et al.,
bioRxiv - Immunology 2024
Quote:
... and reverse transcribed using FIREScript RT cDNA synthesis Kit Solis Biodyne (Lucerna 06 −15-00200) for qPCR analysis ...
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No products found
because this supplier's products are not listed.
A.D. Reed, et al.,
bioRxiv - Microbiology 2021
Quote:
... The full data set was acquired in a randomized fashion with water blanks and system suitability samples (QReSS, Cambridge Isotope Laboratories) collected every 10 samples.
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No products found
because this supplier's products are not listed.
Ian Dardani, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The two readout probes targeting a given amplifier set were pooled together and then coupled to one of four NHS-ester dyes (Atto 488, ATTO-TEC, AD 488-31 ...
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No products found
because this supplier's products are not listed.
Jarrod C. Harman, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Mice were thermoregulated at a body temperature of 37±0.5 °C throughout the procedure using a temp-set YSI 402 micro temperature controller (Yellow Springs Instruments) system with a rectal probe reference ...
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No products found
because this supplier's products are not listed.
Joana Saldida, et al.,
bioRxiv - Systems Biology 2020
Quote:
... The third set of cultures contained a mixture of 80% unlabelled and 20% uniformly labelled [U-13C] glucose (Buchem, CLM-1396). From each flask ...
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No products found
because this supplier's products are not listed.
Kohitij Kar, James J DiCarlo,
bioRxiv - Neuroscience 2020
Quote:
... we used an in-house set up to lower the injection needles (30-32 gauge, small Hub RN Needle; Hamilton Company) using a micro-syringe pump and controller (Micro4™ World Precision Instruments) ...
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No products found
because this supplier's products are not listed.
Na Yang, et al.,
bioRxiv - Genomics 2023
Quote:
... the frozen tissue was ground to a fine powder using a liquid nitrogen cooled mini mortar and pestle set (Bel-Art). The ground tissue was crosslinked first with 3 mM DSG and then with 1% formaldehyde ...
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No products found
because this supplier's products are not listed.
Cassandra Velasco, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... PCR master mixes were decontaminated with double-stranded DNAse treatment (PCR decontamination kit, Arcticzymes, Tromsø, Norway). Sterile water was processed using the same procedure as a negative control ...
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No products found
because this supplier's products are not listed.
Amelia Staats, et al.,
bioRxiv - Microbiology 2022
Quote:
... Inoculated broth cultures were grown for 18 hours at 37°C in an orbital shaker set to 200 RPM (Innova 44; New Brunswick Scientific).
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No products found
because this supplier's products are not listed.
Thomas A. Desautels, et al.,
bioRxiv - Microbiology 2023
Quote:
Immunoassays for screening the first set of 230 designs (Fig. ED1) and later characterization were performed on the Gyrolab xPlore instrument (Gyros Protein Technologies) using the Bioaffy 200 discs (Gyros Protein Technologies) ...
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No products found
because this supplier's products are not listed.
Thomas HB FitzGerald, et al.,
bioRxiv - Neuroscience 2019
Quote:
... agents update beliefs about all states within a fixed-length time window that includes the present time but stretches a set distance into the past (Figure 1) (FitzGerald et al., 2017). This window moves forward in time at the same rate that observations are gathered ...
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No products found
because this supplier's products are not listed.
Richard B. Kennedy, et al.,
bioRxiv - Immunology 2020
Quote:
IFNα and IFNλ production by 2’3’ cGAMP-treated STING-transduced BJAB cells were measured in triplicate using commercial sandwich ELISA assay sets (IFNα: VeriKine-HS™ Human Interferon Alpha All Subtype ELISA Kit, PBL Assay Science; Piscataway ...
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No products found
because this supplier's products are not listed.
Dapeng Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... SMG) and the normal gravity control (NG) were set up in a high-aspect rotating vessel (HARV) bioreactor (Synthecon, Inc., Houston, Texas, USA) and cultured continuously ...
-
No products found
because this supplier's products are not listed.
Etkin Akar, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... then long-term drug responses were recorded every 30 minutes through (RT-CES) (ACEA Biosciences; Agilent Technologies). Cell Index values (CI ...
-
No products found
because this supplier's products are not listed.
Alexander P. Clark, et al.,
bioRxiv - Physiology 2022
Quote:
Patch-clamp measurements were made at a 10 kHz sampling frequency by an amplifier with the low-pass filter set to 5 kHz (Model 2400; A-M Systems, Sequim, WA), and was controlled by the Real Time eXperiment Interface (RTXI ...
-
No products found
because this supplier's products are not listed.
Florencia Rammauro, et al.,
bioRxiv - Microbiology 2024
Quote:
... Cells were further incubated 1 h at RT with monoclonal antibodies anti-p24 (BLV3, 1/200, VMRD, USA). After three washes with PBS ...
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No products found
because this supplier's products are not listed.
Josée Perreault, et al.,
bioRxiv - Immunology 2020
Quote:
... The plates were incubated once again for one hour at RT followed by washing and addition of 100 μl of 3,3’,5,5’-Tetramethylbenzidine (TMB, ESBE Scientific). The colorimetric reaction proceeded for 20 minutes at RT and was stopped by addition of 100 μl of H2SO4 1N (Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Martijn A. Langereis, et al.,
bioRxiv - Microbiology 2021
Quote:
... followed by incubation for 10 minutes at RT with 100 μL/well Super Sensitive TMB (Surmodics, TMBS-1000-01). Reactions were stopped by adding 100 μL/well of 12.5% H2SO4 (Millipore ...
-
No products found
because this supplier's products are not listed.
Christopher Wong, Elena M. Jurczak, Richard Roy,
bioRxiv - Developmental Biology 2023
Quote:
... The gene expression levels were determined by RT-qPCR using a 2x SyberGreen qPCR Master-mix (ZmTech Scientifique, Q2100N) and a Bio-Rad CFX384 Real-Time 96-well PCR qPCR Detection System (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Jimena Leyria, Ian Orchard, Angela B. Lange,
bioRxiv - Molecular Biology 2020
Quote:
... RT-qPCR was performed using an advanced master mix with super green low rox reagent (WISENT Bioproducts Inc, QC, Canada). Three independent experiment were performed (n=3 ...
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No products found
because this supplier's products are not listed.
Anton Sumser, et al.,
bioRxiv - Neuroscience 2022
Quote:
... at room temperature (RT) with constant agitation and subsequently with the primary antibody (Rabbit anti somatostatin, 1:500, BMA Biomedicals; Rabbit anti parvalbumin ...
-
No products found
because this supplier's products are not listed.
Kristin S. Fitzpatrick, et al.,
bioRxiv - Immunology 2022
Quote:
... into empty 96-well low profile PCR plates (Labcon), which were sealed with PCR microplate sealing foil (Eppendorf) ...