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No products found
because this supplier's products are not listed.
Christine E. Nelson, et al.,
bioRxiv - Immunology 2022
Quote:
... and control sets (Diagnostica Stago Inc, Parsippany, NJ). Heart rate ...
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No products found
because this supplier's products are not listed.
Archana Verma, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Primer synthesis was by Reprocell.
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No products found
because this supplier's products are not listed.
Albéric A. de Lajarte, et al.,
bioRxiv - Biochemistry 2024
Quote:
... adding a T7 promoter sequence at the forward primer (5′ TAATACGACTCACTATAG 3′) using a 2X PCR PreMix (Syd Labs, Cat. MB067-EQ2N), human cDNA (ZYAGEN ...
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No products found
because this supplier's products are not listed.
Emily D Hartjes, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... An indoxyl sulfate concentration-response effect on CYP3A4 mRNA expression was generated using a concentration range of indoxyl sulfate found in normal and CKD patients (0 to 1000 µM) with 40 g/L human serum albumin (HSA, Lee Biosolutions, St. Louis, MO). Indoxyl sulfate was incubated in media containing 40 g/L HSA for 3 hours at 37°C to allow for plasma protein binding equilibration prior to cell treatment ...
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No products found
because this supplier's products are not listed.
Qingtao Sun, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and Phosphatase Inhibitor Cocktail Set I (#AA-PHI-I; RayBiotech). Samples were homogenized in Tissue Lyser II (#85300 ...
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No products found
because this supplier's products are not listed.
Ugo Tomasello, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... three sections for each brain electroporated at E14.5 with pUB6-TOM and pSilencer-U6-scram (number of brains, n = 4) or pSilencer-U6-miR-137 (number of brains, n = 4) and injected with retrobeads (Lumafluor) at P9 ...
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No products found
because this supplier's products are not listed.
Sandra L. Bixler, et al.,
bioRxiv - Microbiology 2021
Quote:
Total SARS-CoV-2 E gene and subgenomic E gene target copy numbers were determined by real-time RT-PCR using previously described assays (24–25) and a synthetic RNA containing the subgenomic E RNA sequence (Bio-Synthesis, Inc., Lewisville, TX, USA). Extracted nucleic acid was tested in triplicate (5 μL extracted nucleic acid ...
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No products found
because this supplier's products are not listed.
Seth R. Taylor, et al.,
bioRxiv - Neuroscience 2022
Quote:
... P8 or P14 VGluT2/VGAT-Cre;Ai14;miR-218fl/fl and VGluT2/VGAT-Cre;Ai14;miR-218+/+ mice were rapidly decapitated and brains removed and placed in Hibernate A without Ca2+ (Brainbits #HACA500), supplemented with 0.132 M trehalose and synaptic blockers (AP-V 50 µM ...
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No products found
because this supplier's products are not listed.
Xiaoli Qi, et al.,
bioRxiv - Biochemistry 2023
Quote:
Glutathione probe RT-AM (Kerafast, Cat. No. EBY001)
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No products found
because this supplier's products are not listed.
Ortal Iancu, et al.,
bioRxiv - Bioengineering 2022
Quote:
... using combinations of 4 primers for Vγ and 3 primers for Jγ regions in each reaction (IdentiClone™ TCRG Gene Clonality Assay, Invivoscribe, Inc.). TRG clonality was ran and analyzed on 2% agarose gel ...
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No products found
because this supplier's products are not listed.
Dennis Alejandro Escolástico-Ortiz, et al.,
bioRxiv - Microbiology 2023
Quote:
... the forward primer Ueda19F (GCI WTY TAY GGI AAR GGI GG) and the reverse primer R6 (GCC ATC ATY TCI CCI GA). The oligonucleotide sequences used for amplification included the selected primers and specific sample indexes conceived for Illumina sequencing.
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Magnetofection
Cat# IV-MAG1,
contains 1 extra small cylinder (ø 2 mm), 1 small cylinder (ø 5 mm), 1 cylinder (ø 10 mm) and 1 square (18x18 mm) magnets, USD $69.00/KIT
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Allison Ogren, et al.,
bioRxiv - Genetics 2019
Quote:
... The culture plate was set on top of a plate magnet (Oz Biosciences) for 20 min and returned to the incubator ...
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No products found
because this supplier's products are not listed.
Tamar (Skaist) Mehlman, et al.,
bioRxiv - Biophysics 2022
Quote:
... we used fragments from the Maybridge 1000 fragment library (Maybridge Ro3 core set), the Edelris Keymical fragment library ...
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The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically...
Cat# B40015, SKU# B40015-500rxns,
500rxns, $267.00
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Suman Khan, et al.,
bioRxiv - Microbiology 2023
Quote:
... The composition of the screening set was 0.7% Bioactive collections (Selleck Chemicals, USA), 7.6% HitFinder (Maybridge ...
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No products found
because this supplier's products are not listed.
Rebecca E. Poulsen, et al.,
bioRxiv - Neuroscience 2020
Quote:
6dpf larval zebrafish were set in a 2% low melting point agarose (Progen Biosciences), dorsal side up ...
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No products found
Shuangfen Tao, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... We purchased Target sequences for Bcl-2 miRNA 3’-UTR clone and the one with a site mutation at miR-383-binding site from Creative Biogene (Shirley, NY, USA). We seeded MiR-383-modified cells of GC in 24-well plates ...
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No products found
because this supplier's products are not listed.
Matteo Chighizola, et al.,
bioRxiv - Biophysics 2022
Quote:
... for 30 min at RT) glass-bottomed plates (Willco Wells). The fixed cells were characterised using a Bioscope Catalyst AFM from Bruker ...
-
No products found
because this supplier's products are not listed.
Nathan M. Good, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Extracts were prepared as described using an OS Cell Disrupter set at 25,000 psi (Constant Systems Limited ...
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No products found
because this supplier's products are not listed.
Sudhir Kumar, et al.,
bioRxiv - Microbiology 2021
Quote:
... Gametocyte cultures were set up in 6 well plates using O+ human RBCs (Valley Biomedical, VA, US) and O+ human serum (Interstate Blood Bank ...
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No products found
because this supplier's products are not listed.
Danielle L. Schmitt, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... All filter sets were controlled by an external filter-exchanger (Prior Scientific or Ludl Electronic Products, Ltd). Exposure times ranged between 50 to 100 ms ...
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No products found
because this supplier's products are not listed.
Katie E. Blise, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Single marker positivity thresholds were set using FCS Express Image Cytometry RUO (De Novo Software, Glendale, CA) to visually validate protein biomarker expression overlaid on signal extracted images ...
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No products found
Ons Mamai, et al.,
bioRxiv - Cell Biology 2022
Quote:
... After blocking the membrane for 1h at RT with AdvanBlock (Advansta®), and proteins were detected by incubation with relevant antibodies (Supplementary Table S6 ...
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No products found
because this supplier's products are not listed.
Petra Hafker, et al.,
bioRxiv - Physiology 2021
Quote:
... we used a benchtop chiller with an antifreeze bath set to 1°C (MicroCooler II Model 260010, Boekel Scientific ...
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No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Alternating tangential flow was set to 0.11 L/min (5 L/m2/min) using an XCell Lab Controller (Repligen). The desired rate of medium exchange was achieved by controlling the flow rate of the permeate (spent medium ...
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No products found
because this supplier's products are not listed.
Jiling Feng, Yuexun Tang, Wenwei Fu, Hongxi Xu,
bioRxiv - Cell Biology 2023
Quote:
... RT-PCR was performed with a one-step real time PCR using KAPA SYBR FAST One-Step qRT-PCR Universal (D-MARK Biosciences). Primers were used as previously described [18] ...
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No products found
because this supplier's products are not listed.
Courtney A. Copeland, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Hypoxia-treated cells were transferred to a tissue culture glovebox set at 0.5% oxygen and 5% CO2 (Coy Lab Products). Medium was supplemented with DMSO 0.1% or BAY (10 μM ...
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No products found
because this supplier's products are not listed.
Kristin L. Rosche, et al.,
bioRxiv - Microbiology 2023
Quote:
... cells were transfected with 0.05µg of each vector from the UPR/ER stress response luciferase reporter vector set (Signosis, LR-3007) and 0.5µl of Lipofectamine 2000 in Opti-MEM I reduced-serum medium (Gibco ...
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No products found
because this supplier's products are not listed.
Jan Zlamal, et al.,
bioRxiv - Immunology 2022
Quote:
... a calcium independent ligand of PS (200nM, 15 min, RT; Haematologic Technologies, Essex Junction, USA).29,30 Sufficient blocking concentrations of Lactadherin were assessed via Calibrated Automated Thrombogram (CAT ...
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No products found
because this supplier's products are not listed.
R. Doolan, et al.,
bioRxiv - Immunology 2024
Quote:
... and reverse transcribed using FIREScript RT cDNA synthesis Kit Solis Biodyne (Lucerna 06 −15-00200) for qPCR analysis ...
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No products found
because this supplier's products are not listed.
A.D. Reed, et al.,
bioRxiv - Microbiology 2021
Quote:
... The full data set was acquired in a randomized fashion with water blanks and system suitability samples (QReSS, Cambridge Isotope Laboratories) collected every 10 samples.
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No products found
because this supplier's products are not listed.
Ian Dardani, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The two readout probes targeting a given amplifier set were pooled together and then coupled to one of four NHS-ester dyes (Atto 488, ATTO-TEC, AD 488-31 ...
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No products found
because this supplier's products are not listed.
Joana Saldida, et al.,
bioRxiv - Systems Biology 2020
Quote:
... The third set of cultures contained a mixture of 80% unlabelled and 20% uniformly labelled [U-13C] glucose (Buchem, CLM-1396). From each flask ...
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No products found
because this supplier's products are not listed.
Na Yang, et al.,
bioRxiv - Genomics 2023
Quote:
... the frozen tissue was ground to a fine powder using a liquid nitrogen cooled mini mortar and pestle set (Bel-Art). The ground tissue was crosslinked first with 3 mM DSG and then with 1% formaldehyde ...
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No products found
because this supplier's products are not listed.
Amelia Staats, et al.,
bioRxiv - Microbiology 2022
Quote:
... Inoculated broth cultures were grown for 18 hours at 37°C in an orbital shaker set to 200 RPM (Innova 44; New Brunswick Scientific).
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No products found
because this supplier's products are not listed.
Thomas A. Desautels, et al.,
bioRxiv - Microbiology 2023
Quote:
Immunoassays for screening the first set of 230 designs (Fig. ED1) and later characterization were performed on the Gyrolab xPlore instrument (Gyros Protein Technologies) using the Bioaffy 200 discs (Gyros Protein Technologies) ...
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No products found
because this supplier's products are not listed.
Thomas HB FitzGerald, et al.,
bioRxiv - Neuroscience 2019
Quote:
... agents update beliefs about all states within a fixed-length time window that includes the present time but stretches a set distance into the past (Figure 1) (FitzGerald et al., 2017). This window moves forward in time at the same rate that observations are gathered ...
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No products found
because this supplier's products are not listed.
Etkin Akar, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... then long-term drug responses were recorded every 30 minutes through (RT-CES) (ACEA Biosciences; Agilent Technologies). Cell Index values (CI ...
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No products found
because this supplier's products are not listed.
Florencia Rammauro, et al.,
bioRxiv - Microbiology 2024
Quote:
... Cells were further incubated 1 h at RT with monoclonal antibodies anti-p24 (BLV3, 1/200, VMRD, USA). After three washes with PBS ...
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No products found
because this supplier's products are not listed.
Josée Perreault, et al.,
bioRxiv - Immunology 2020
Quote:
... The plates were incubated once again for one hour at RT followed by washing and addition of 100 μl of 3,3’,5,5’-Tetramethylbenzidine (TMB, ESBE Scientific). The colorimetric reaction proceeded for 20 minutes at RT and was stopped by addition of 100 μl of H2SO4 1N (Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Martijn A. Langereis, et al.,
bioRxiv - Microbiology 2021
Quote:
... followed by incubation for 10 minutes at RT with 100 μL/well Super Sensitive TMB (Surmodics, TMBS-1000-01). Reactions were stopped by adding 100 μL/well of 12.5% H2SO4 (Millipore ...
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No products found
because this supplier's products are not listed.
Christopher Wong, Elena M. Jurczak, Richard Roy,
bioRxiv - Developmental Biology 2023
Quote:
... The gene expression levels were determined by RT-qPCR using a 2x SyberGreen qPCR Master-mix (ZmTech Scientifique, Q2100N) and a Bio-Rad CFX384 Real-Time 96-well PCR qPCR Detection System (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Jimena Leyria, Ian Orchard, Angela B. Lange,
bioRxiv - Molecular Biology 2020
Quote:
... RT-qPCR was performed using an advanced master mix with super green low rox reagent (WISENT Bioproducts Inc, QC, Canada). Three independent experiment were performed (n=3 ...
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No products found
because this supplier's products are not listed.
Anton Sumser, et al.,
bioRxiv - Neuroscience 2022
Quote:
... at room temperature (RT) with constant agitation and subsequently with the primary antibody (Rabbit anti somatostatin, 1:500, BMA Biomedicals; Rabbit anti parvalbumin ...
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No products found
because this supplier's products are not listed.
Leanne E. Wybenga-Groot, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and 0.5 mM Na3VO4) at rt for 5-10 min before addition of master mix (42 pmol E1 (UBE1, Boston Biochem or Ubiquitin-Proteasome Biotechnologies) ...
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No products found
because this supplier's products are not listed.
Kristin S. Fitzpatrick, et al.,
bioRxiv - Immunology 2022
Quote:
... into empty 96-well low profile PCR plates (Labcon), which were sealed with PCR microplate sealing foil (Eppendorf) ...
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No products found
because this supplier's products are not listed.
John Paul Tsu Ouyang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Samples were then shaken at 1500 RPM for five minutes at RT in a benchtop shaker (Benchmark Scientific; Model #: H5000-HC). Samples were then incubated for 5 minutes at RT with no shaking ...
-
No products found
because this supplier's products are not listed.
Ester Orav, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Sections were incubated for 24-72 hrs at RT with rabbit anti-5-HT primary antibody (1:1000, cat no 20080, Immunostar, USA) in carrier solution containing 1% NGS ...
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No products found
because this supplier's products are not listed.
Tatiana Agback, et al.,
bioRxiv - Molecular Biology 2021
Quote:
The PCR product was cloned into pE-SUMOpro-3 plasmid (LifeSensors Inc) between Bsa I and Xho I restriction sites ...
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No products found
because this supplier's products are not listed.
Lucas Caldi Gomes, et al.,
bioRxiv - Neuroscience 2024
Quote:
... C9orf72 repeat expansion analysis was performed using the AmplideX PCR/CE C9orf72 Kit (Asuragen, Austin, TX, USA). Briefly ...
-
No products found
because this supplier's products are not listed.
Mindaugas Zaremba, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... was amplified by PCR from the genomic DNA of Geobacter sulfurreducens Caccavo (ATCC No. 51573, LGC Standards cat#51573D-5) using the oligonucleotides MZ-239 and MZ-240 (Supplementary Table 2) ...