-
No products found
because this supplier's products are not listed.
Anne-Catherine Fluckiger, et al.,
bioRxiv - Immunology 2019
Quote:
... for detection of ZIKV E and mouse anti-Zika NS1 mAb (BioFront Technologies) as primary Abs ...
-
No products found
because this supplier's products are not listed.
David S. Tourigny, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Signal amplification was obtained by HiDef Detection HRP Polymer System (954D-50, Medac Diagnostika, Germany) followed by DAB substrate kit (957D-30 ...
-
No products found
because this supplier's products are not listed.
Jan Zlamal, et al.,
bioRxiv - Immunology 2022
Quote:
... a calcium independent ligand of PS (200nM, 15 min, RT; Haematologic Technologies, Essex Junction, USA).29,30 Sufficient blocking concentrations of Lactadherin were assessed via Calibrated Automated Thrombogram (CAT ...
-
No products found
because this supplier's products are not listed.
Wim J. de Jonge, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... by bead beating 7 times 3 minutes in a Genie Disruptor (Scientific Industries). The lysate was recovered and centrifuged at 1503g for 2 minutes at 4°C to remove cell debris ...
-
No products found
because this supplier's products are not listed.
Ayat Zawawi, et al.,
bioRxiv - Immunology 2019
Quote:
... The level of endotoxin in all the purified VLPs was measured with an ELISA-based endotoxin detection assay (Hyglos) following the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
María A. Duque-Correa, et al.,
bioRxiv - Immunology 2020
Quote:
... Organoids were washed six times with PBS and incubated with FocusClear™ (CelExplorer Labs.) at RT for 1-2 h ...
-
No products found
because this supplier's products are not listed.
Tongcui Ma, et al.,
bioRxiv - Immunology 2021
Quote:
... Cells were then washed three times with CyFACS buffer and stained with Maleimide DOTA (Macrocyclics) for 30 min on ice ...
-
No products found
because this supplier's products are not listed.
Colin J. Raposo, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Signal on the blot was detected with Amersham ECL Prime Western Blotting Detection Reagent (GE Lifesciences) and imaged with autoradiography film (Andwin Scientific).
-
No products found
because this supplier's products are not listed.
Regina M. Powers, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and incubated with the following primary antibodies diluted in PBS for 1hr at RT: TuJ1 (1:2000 to stain primary neurons, 1:300 to stain SH-SY5Y cells, Dr. A. Frankfurter, and Neuromics MO15013), anti-Nav1 (generation described above ...
-
No products found
because this supplier's products are not listed.
Johanna Hol Fosse, et al.,
bioRxiv - Microbiology 2023
Quote:
... Acetone-fixed cells were incubated with IgG1 against the ISAV nucleoprotein (P10, Aquatic Diagnostics Ltd, 0.4 μg/mL, 60 min, RT), washed (PBS × 3) ...
-
No products found
because this supplier's products are not listed.
Linda M. Sircy, et al.,
bioRxiv - Immunology 2023
Quote:
... virus strain for 30min at room temperature (RT) prior to addition of 50μL of 1% chicken red blood cells (cRBCs) (Lampire Biological Laboratories) in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Ana BF Emiliano, et al.,
bioRxiv - Physiology 2020
Quote:
... saline and enrofloxacin subcutaneously at the time of the surgery (Boehringer Ingelheim; saline from Medline; Bayer). A midline laparotomy was performed and the stomach isolated from surrounding connective tissue ...
-
No products found
because this supplier's products are not listed.
Juan Martín D’Ambrosio, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 1.5 μl for bound franction for GFP detection) were loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
-
No products found
because this supplier's products are not listed.
Alexis Bouin, et al.,
bioRxiv - Microbiology 2022
Quote:
... diluted 1:500 in blocking solution for detection of viral protein VP1 or anti dsRNA antibody (SCICONS, anti-dsRNA mAb J2, 10010500) diluted 1:1000 in blocking solution overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Aliya Sharipova, et al.,
bioRxiv - Bioengineering 2022
Quote:
... the Fe with 1wt% VH precursor mixture was loaded into a custom-build consolidation die and high-pressure cold-sintered at 2.5 GPa (corresponding to 5 t for 5 mm diameter die) and RT using manual press (Carver, Wabash, IN, USA) to obtain the drug-loaded metal (Supplementary Materials ...
-
No products found
because this supplier's products are not listed.
Sonia Ponzo, et al.,
bioRxiv - Neuroscience 2019
Quote:
... to check for variability over time and ii) a one-way (GVS: LGVS vs. RGVS vs. Sham) ANOVA on the averaged proprioceptive drift values of the two visual capture conditions ...
-
No products found
because this supplier's products are not listed.
Charlotte Repton, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Ovaries from 3-day matured flies were dissected one at a time in Halocarbon oil (700; Halocarbon) on a cover slip ...
-
No products found
because this supplier's products are not listed.
Timothy M. Ghaly, et al.,
bioRxiv - Microbiology 2021
Quote:
... All PCRs were performed using GeneReleaser® (Bioventures, Murfreesboro, TN, USA) as previously described [32] ...
-
No products found
because this supplier's products are not listed.
Pedro Trevizan-Baú, et al.,
bioRxiv - Neuroscience 2020
Quote:
... followed by incubation in hydrogen peroxide for 20 min and incubated for 24 h at room temperature (RT) with a goat anti-CT-B antibody (1:10,000 List Biological Laboratories, CA, USA). Then ...
-
No products found
because this supplier's products are not listed.
Abdoulie O. Touray, et al.,
bioRxiv - Microbiology 2023
Quote:
... Membranes were probed for 2 h at RT (or overnight at 4°C) with mAb α-V5 (BioShop Canada Inc., catalog number TAG006.100) 1:2,500 in 6% milk in PBS 0.05% Tween (PBS-T) ...
-
No products found
because this supplier's products are not listed.
Stephan Brouwer, et al.,
bioRxiv - Microbiology 2020
Quote:
... The primary antibodies used for the detection of SpeC and SSA protein in GAS culture supernatants were rabbit antibody to SpeC (PCI333, Toxin Technology; 1:1,000 dilution) and affinity-purified rabbit antibody to SSA (produced by Mimotopes ...
-
No products found
because this supplier's products are not listed.
Ji Zhang, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... and Primary Human IPF Lung Parenchymal Fibroblasts (Donor2) (BioIVT, #PCR-70-0214) were incubated at 37°C and 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Emily M. Mace, et al.,
bioRxiv - Immunology 2019
Quote:
... selected with puromycin and confirmed by PCR for SV40 expression (Applied Stemcell). 293T fibroblast lines were maintained in DMEM supplemented with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Zeru Tian, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cells were washed three times with PBS (pH 7.4) and then incubated with DiIC18(3) (Marker Gene Technologies, Inc.) for 20 min and Hoechst 33342 (Cat No ...
-
No products found
because this supplier's products are not listed.
Antonio Frasca, et al.,
bioRxiv - Bioengineering 2020
Quote:
8-10 mm discs of BP were rinsed 3 times in 0.9% saline (Rocky Mountain Biologicals, Missoula, MT, USA) and then incubated for 24 hours at 37°C in 0.9% saline ...
-
No products found
because this supplier's products are not listed.
Rodolpho Ornitz Oliveira Souza, et al.,
bioRxiv - Microbiology 2024
Quote:
... Samples were washed five times with PBST and resuspended in 100 µL containing 1mM biotin phenol (Iris Biotech GmbH) for 10 minutes and then mixed with the previous solution ...
-
No products found
because this supplier's products are not listed.
RA Seaborne, et al.,
bioRxiv - Physiology 2019
Quote:
... UBR5 PCR primers and pyrosequencing primers were purchased from EpigenDX (Hopkinton, MA, USA), rat assay no ...
-
No products found
because this supplier's products are not listed.
Nádia Rei, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 60-100 µg protein/well of cortex and 40-60 µg protein/well of spinal cord were incubated at RT for 2 hours with the selective high affinity A1R antagonist [3H]DPCPX ([3H]-8-Cyclopentyl-1,3-dipropylxanyine; American Radiolabeled Chemicals, Inc., St. Louis, MO, EUA) and 4 U/mL of Adenosine Deaminase (ADA ...
-
No products found
because this supplier's products are not listed.
D.M.K. Aladin, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The dissociated cells were gently pipetted 5 times to dissociate cell clumps before being deposited on a PLL-PEG-coated (SuSoS), glass-bottomed dish (IWAKI) ...
-
No products found
because this supplier's products are not listed.
Nika Pende, et al.,
bioRxiv - Microbiology 2020
Quote:
... 50 mL of the medium were aliquoted into serum bottles and sealed with blue rubber stoppers (pretreated by boiling ten times for 30 min in fresh ddH2O; 20 mm, Bellco) and open-top aluminum caps (9.5 mm opening ...
-
No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
-
No products found
because this supplier's products are not listed.
Mafalda Cacciottolo, et al.,
bioRxiv - Immunology 2023
Quote:
Fresh plasma at the time of terminal collection was analyzed according to the manufacturer’s instructions using the Hemavet 950FS (Drew Scientific).
-
No products found
because this supplier's products are not listed.
Guillaume Jacquemin, et al.,
bioRxiv - Cell Biology 2021
Quote:
Muscle injury was performed in Pax7CreERT2;R26mTmG adult mice at the time of PDMS-imaging window implantation by intramuscular injection of 50 μl of Cardiotoxin (10 μM, Latoxan, L8102) diluted in 0.9% NaCl ...
-
No products found
because this supplier's products are not listed.
Benjamin Souferi, Mark Emerson,
bioRxiv - Developmental Biology 2019
Quote:
... Cells were washed three times in 1 ml of 1X PBS upon being filtered through a 40μm strainer (Biologix, 15-1040). All centrifuge spins were at 1,700rcf for 5 minutes and supernatant removed with a P1000 pipettor.
-
No products found
because this supplier's products are not listed.
R. Stefan Isaac, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cells were washed three times with 1X PBS and the chamber slide was incubated in a hybridization oven (Boekel Scientific, RapidFISH) prewarmed to 37°C ...
-
No products found
because this supplier's products are not listed.
Allison V. Banse, et al.,
bioRxiv - Microbiology 2022
Quote:
... The resin was washed three times with PBS to remove unbound bacteria and finally resuspended in PBS supplemented with 0.4% GlcNAc (Chem-Impex #01427) to release bacteria attached to the resin ...
-
No products found
because this supplier's products are not listed.
Kärt Mätlik, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The cerebella were soaked in the DNA for 15-20 minutes on ice and were transferred one at a time into the well of an electroporation chamber (Protech International Inc ...
-
No products found
because this supplier's products are not listed.
Sarah Thorwall, Varun Trivedi, Ian Wheeldon,
bioRxiv - Systems Biology 2023
Quote:
... PCA and PCN peaks were identified by comparing retention times to those of purchased PCA and PCN (ChemScene; Monmouth Junction, NJ). Because pure 2-HP and 2-HPCA were not commercially available ...
-
No products found
because this supplier's products are not listed.
Grzegorz Maciag, et al.,
bioRxiv - Cell Biology 2024
Quote:
... After antibody staining the tissue was washed 6 times in PBS and before imaging the tissue was incubated in RapiClear (SUNJin Lab) for 30-60 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Paul Mollenkopf, et al.,
bioRxiv - Biophysics 2021
Quote:
... These strands were hybridized in a Professional Standard PCR Thermocycler (Core Life Sciences Inc., USA) by denaturation for 10min at 90°C and complementary base pairing for 20h between 80° C and 20° C ...
-
No products found
because this supplier's products are not listed.
Du-Hwa Lee, et al.,
bioRxiv - Plant Biology 2022
Quote:
... RealHelixTM qPCR kit (NANOHELIX; Korea), and the StepOnePlus Realtime PCR System (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Angus M Sidore, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... The individual wells were then washed >5 times using 2X Bind & Wash Buffer and a 384-Well Post Magnetic Plate (Permagen Labware, Peabody, MA). After washing ...
-
No products found
because this supplier's products are not listed.
Renato M. Rossi, et al.,
bioRxiv - Immunology 2022
Quote:
... Samples of all total mixed rations were collected at 2-weeks intervals from the feed bunk at the time of distribution and sent to an external laboratory for chemical composition analysis (Cumberland Valley Analytical Services, Waynesboro, PA). The composition and chemical analysis results of the diets are summarized in Supplementary Tables S1 and S2.
-
No products found
because this supplier's products are not listed.
Sadaf N Kalam, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Tissue was rinsed again in dH20 three times for 5 min and dehydrated with a graded series of ethanol then infiltrated with Spurr’s resin (SPI supplies, Leicestershire, England, UK). Samples were embedded in fresh Spurr’s resin in BEEM® capsules (ProSciTech ...
-
No products found
Fakhriedzwan Idris, et al.,
bioRxiv - Microbiology 2024
Quote:
... three times (weeks 0, 2, and 6) with 20 µg of purified hexameric NS1 from DENV2 D2Y98P strain (custom-made, The Native Antigen Company) or OVA protein (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Gyula Batta, et al.,
bioRxiv - Cell Biology 2020
Quote:
... After spectral compensation and gating out dead cells based on DAPI positivity the time-correlated fluorescence intensities were exported using FCS Express (De Novo Software, Pasadena, CA). A custom-written Matlab program was used for calculating a moving average with a window size of 20 seconds ...
-
No products found
because this supplier's products are not listed.
Drake Jensen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... These templates underwent an additional PCR step with Klentaq LA DNA Polymerase (84) (DNA Polymerase Technology; catalog # 110), using a biotinylated primer (Integrated DNA Technologies ...
-
No products found
because this supplier's products are not listed.
Stephanie N. Caty, et al.,
bioRxiv - Microbiology 2024
Quote:
... frogs were rinsed with sterile water and then swabbed 10 times (up and back is once) on the dorsal side using Puritan Sterile Tipped Polyester Applicators (Puritan Medical Products, 25-206 1PD BT). Alkaloid feeding began 5 days after the onset of swabbing (Day 13 ...
-
No products found
because this supplier's products are not listed.
Aggeliki Tserga, et al.,
bioRxiv - Systems Biology 2021
Quote:
... Urinary albumin concentration was measured by ELISA using the AlbuWell kit (WAK-Chemie Medical GmbH, Steinbach, Germany). Urinary creatinine concentration was measured by the colorimetric method of Jaffe ...
-
No products found
because this supplier's products are not listed.
Hongji Yan, et al.,
bioRxiv - Bioengineering 2019
Quote:
The sialic acid removal assay was conducted by using Neuraminidase immobilized on slurry (GlycoCleave Neuraminidase kit, GALAB technologies). Briefly ...