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No products found
because this supplier's products are not listed.
Raza Ali Naqvi, Araceli Valverde, Afsar Naqvi,
bioRxiv - Immunology 2023
Quote:
... Gene expression was examined using PCR array plate containing 88 primer sets directed against human NF-κB pathway genes and 8 housekeeping primer sets (Real Time Primers, LLC Elkins Park, PA). Briefly ...
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No products found
because this supplier's products are not listed.
Emily D Hartjes, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... An indoxyl sulfate concentration-response effect on CYP3A4 mRNA expression was generated using a concentration range of indoxyl sulfate found in normal and CKD patients (0 to 1000 µM) with 40 g/L human serum albumin (HSA, Lee Biosolutions, St. Louis, MO). Indoxyl sulfate was incubated in media containing 40 g/L HSA for 3 hours at 37°C to allow for plasma protein binding equilibration prior to cell treatment ...
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No products found
because this supplier's products are not listed.
Ugo Tomasello, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... three sections for each brain electroporated at E14.5 with pUB6-TOM and pSilencer-U6-scram (number of brains, n = 4) or pSilencer-U6-miR-137 (number of brains, n = 4) and injected with retrobeads (Lumafluor) at P9 ...
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No products found
because this supplier's products are not listed.
Nora Hartig, Kathrin M. Seibt, Tony Heitkam,
bioRxiv - Plant Biology 2022
Quote:
... PCR products were purified (As One International,Invisorb Spin DNA Extraction Kit) and ligation into pGEM-T plasmids (ThermoFisher Scientific ...
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No products found
Michael L. Nosella, et al.,
bioRxiv - Biochemistry 2021
Quote:
... with the exception that the alkyne-biotin detection reagent from the latter kit was replaced with methoxy-PEG-alkyne (average MW ∼5kDa; Biochempeg, Watertown, MA, USA), which was present at a final concentration of 5mM during the Click chemistry step ...
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This kit is used for distinguishing between healthy and apoptotic cells by detecting the changes...
Cat# KITA1006,
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Shuangfen Tao, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... We purchased Target sequences for Bcl-2 miRNA 3’-UTR clone and the one with a site mutation at miR-383-binding site from Creative Biogene (Shirley, NY, USA). We seeded MiR-383-modified cells of GC in 24-well plates ...
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No products found
because this supplier's products are not listed.
Florencia Rammauro, et al.,
bioRxiv - Microbiology 2024
Quote:
... Cells were further incubated 1 h at RT with monoclonal antibodies anti-p24 (BLV3, 1/200, VMRD, USA). After three washes with PBS ...
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No products found
because this supplier's products are not listed.
Josée Perreault, et al.,
bioRxiv - Immunology 2020
Quote:
... The plates were incubated once again for one hour at RT followed by washing and addition of 100 μl of 3,3’,5,5’-Tetramethylbenzidine (TMB, ESBE Scientific). The colorimetric reaction proceeded for 20 minutes at RT and was stopped by addition of 100 μl of H2SO4 1N (Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Anton Sumser, et al.,
bioRxiv - Neuroscience 2022
Quote:
... at room temperature (RT) with constant agitation and subsequently with the primary antibody (Rabbit anti somatostatin, 1:500, BMA Biomedicals; Rabbit anti parvalbumin ...
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No products found
because this supplier's products are not listed.
Justin A. Bosch, et al.,
bioRxiv - Genomics 2023
Quote:
... Briefly, embryos from each time period (i.e., sample) were treated with harringtonine (LKT Laboratories, H0169) in mild lysis buffer followed by the addition of cycloheximide and immediate grinding ...
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No products found
because this supplier's products are not listed.
A Elgheznawy, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Platelets were activated with appropriately diluted agonists for 8 min at 37°C followed by 8 min at RT in the presence of saturating amounts of PE-coupled JON/A (4H5, Emfret Analytics) detecting activated αIIbβ3 integrin and FITC-coupled anti-P-selectin (Wug.E9 ...
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No products found
because this supplier's products are not listed.
Vipul Sharma, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... amplified using Taq Polymerase 2X PCR premix (Intact Genomics, 3249) and SX primers (SX_F ...
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No products found
because this supplier's products are not listed.
Juliana Bernardi Aggio, et al.,
bioRxiv - Immunology 2021
Quote:
Neutrophils and A549 cells viability were determined at the indicated time points using Annexin V (ImmunoTools) and 7-Amino-Actinomycin (7-AAD ...
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No products found
because this supplier's products are not listed.
Ragna S. Häussler, et al.,
bioRxiv - Biochemistry 2019
Quote:
... the antibody-coupled beads were washed three times in 100 μl 1x PBS (09-9400, Medicago), 0.05% Tween20 (BP337 ...
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No products found
because this supplier's products are not listed.
Jan Zlamal, et al.,
bioRxiv - Immunology 2022
Quote:
... was blocked via the pretreatment of PLTs with 5 μg/mL anti-SELP humanized Ab (anti-CD62p, 15 min, RT; ProteoGenix, Schiltigheim, France). Using a modified FC approach that detects PLT-leukocyte aggregates ...
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No products found
because this supplier's products are not listed.
Sangderk Lee, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Cells were sequentially filtered two more times using Flowmi cell strainers (70μm pore size, Bel-Art #H13680-0040). Cell counts and viability were assessed using AO/PI cell viability dyes (Logos Biosystems #F23001 ...
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No products found
because this supplier's products are not listed.
Zi Ye, et al.,
bioRxiv - Neuroscience 2021
Quote:
... in PBST was applied and the slides were blocked in the dark at RT for 1h in HybriWell sealing chambers (Grace Bio-Labs, Bend, OR). Primary antibody Rabbit α-Orco was diluted 1:500 in 5% normal goat serum in PBST and applied on the slides and incubated overnight at 4°C.
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No products found
because this supplier's products are not listed.
Marek Harhala, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Then the slurry was washed with 10x of 85 mM imidazole in PBS and the active protein was eluted with 250 mM imidazole and dialyzed three times against 100-fold excess of PBS at 4 °C (3,000 kDa molecular weight cut-off, Spectra/Por, Repligen). Finally ...
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No products found
because this supplier's products are not listed.
S. Francesca Antillon, et al.,
bioRxiv - Microbiology 2023
Quote:
... and at the time of expected lysis .At each time point 1.5µL samples were put onto a 25 × 75 x 1mm glass slide (Globe Scientific) and covered with a 22 × 22mm cover slip (Globe Scientific ...
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No products found
because this supplier's products are not listed.
Cameron Moshfegh, et al.,
bioRxiv - Cell Biology 2021
Quote:
... PCR Primers were designed using PrimerSelect from the Lasergene software package (DNASTAR, USA). All primers were synthesized at Microsynth ...
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No products found
because this supplier's products are not listed.
Kathryn Stewart, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... after which the samples were homogenized 3 times for 30s at 6500 rpm using a Precellys24 tissue homogenizer (Bertin Corp). Upon homogenization ...
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No products found
because this supplier's products are not listed.
Felix Clemens Richter, et al.,
bioRxiv - Immunology 2022
Quote:
... FAMEs were identified routinely by comparing retention times of peaks with those of G411 FA standards (Nu-Chek Prep Inc). TotalChrom software (Perkin-Elmer ...
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No products found
because this supplier's products are not listed.
Tomás Urzúa Lehuedé, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Seeds were then rinsed five times with sterile water and placed uniformly across ½ Murashige and Skoog (MS; PhytoTechnology laboratories, USA), 1% sucrose ...
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No products found
because this supplier's products are not listed.
Nirmitee Mulgaonkar, et al.,
bioRxiv - Microbiology 2020
Quote:
... cells were washed three times with OptiMEM and replaced with medium containing anti-VSV-G neutralizing antibody (clone 8G5F11; Absolute Antibody) at a dilution of 1:50,000 to block remaining VSV-G pseudovirus ...
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No products found
because this supplier's products are not listed.
Chanese A. Forté, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Air samples were collected during the time farmers were spraying pesticides (14 to 63 minutes) using XAD-2 sorbent tubes (SKC Ltd) based on the standard NIOSH method of 5600 at a flowrate of one liter per minute(20) ...
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No products found
because this supplier's products are not listed.
Martin F. Peter, et al.,
bioRxiv - Biophysics 2020
Quote:
... the protein eluate was treated with 5 times excess per cysteine of the nitroxide spin label MTSSL (Toronto Research Chemicals, Canada), dissolved in DMSO ...
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No products found
because this supplier's products are not listed.
William Lee, et al.,
bioRxiv - Molecular Biology 2023
Quote:
TOM20 and H2B Type 1A ORFs were amplified by PCR from human heart cDNA (Zyagen). The amplicons were cloned into the pT7Blue cloning vector (Novagen ...
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No products found
because this supplier's products are not listed.
Daniel D. Lam, et al.,
bioRxiv - Genetics 2021
Quote:
GABAergic neurons and glutamatergic neurons (iCell GABANeurons Kit and iCell GlutaNeurons Kit) were purchased from Cellular Dynamics. iCell GABANeurons and GlutaNeurons are highly pure populations of human neurons ...
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No products found
because this supplier's products are not listed.
Ho-Shiang Huang, Chan-Jung Liu,
bioRxiv - Biochemistry 2021
Quote:
Commercial kits were used to determine the urine level of NGAL (NGAL ELISA Kit, BioPorto Diagnostics A/S, Copenhagen, Denmark) and the urine levels of stone-induced renal tubular damage markers ...
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No products found
because this supplier's products are not listed.
Mindaugas Zaremba, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... was amplified by PCR from the genomic DNA of Geobacter sulfurreducens Caccavo (ATCC No. 51573, LGC Standards cat#51573D-5) using the oligonucleotides MZ-239 and MZ-240 (Supplementary Table 2) ...
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No products found
because this supplier's products are not listed.
Adil R Wani, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 4 transgenic lines were identified through the loss of y and were PCR-tested for orientation of insertion by Bestgene. We then tested these lines for expression of TdTomato and gene disruption after conditional expression of FLP Recombinase (Figure S3A-D) ...
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No products found
because this supplier's products are not listed.
Adebayo O. Shittu, Tomiwa Adesoji, Edet E. Udo,
bioRxiv - Microbiology 2020
Quote:
... aureus genotyping Kit 2.0 (Alere Technology, Jena, Germany) as described previously [10] ...
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No products found
because this supplier's products are not listed.
Kathleen E. DelGiorno, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... slides were stained using a kit (IHC world) according to the manufacturer’s instructions ...
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Detect mature miRNA hsa-mir-296-5p expression.
Cat# CPK1164,
50 rxns USD $200.0, 100 rxns USD $330.0, 200 rxns USD $530.0
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Pierick Mouginot, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... MDA concentrations were determined using the commercial kit MDA Microplate Assay Kit (Cat. no. CAK1011; Cohesion Bioscience; 532 and 600 nm). GSH levels were assessed with spectrophotometric method ...
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No products found
because this supplier's products are not listed.
Edward Sullivan, et al.,
bioRxiv - Microbiology 2021
Quote:
The BioSensor SARS-CoV-2 Ag Kit (Oxford Biosystems) was used in accordance with manufacturer’s instructions to process swab samples from hamsters ...
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No products found
because this supplier's products are not listed.
Pauline Bohne, et al.,
bioRxiv - Neuroscience 2023
Quote:
... connected to the ‘Brain Infusion Kit 3’ (#0004760, Durect). Pumps were prepared sterilely according to the manual ...
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No products found
because this supplier's products are not listed.
Eun Jeong Lee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Plasma ACTH concentrations were measured using the ACTH ELISA kit (MD Biosciences), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Tomasz Zieliński, et al.,
bioRxiv - Biophysics 2022
Quote:
... CytoGlow™ Cofilin (Phospho-Ser3) Colorimetric Cell-Based ELISA Kit was applied (Assay Biotechnology) to monitor target proteins concentration ...
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No products found
because this supplier's products are not listed.
S. Gulberk Ozcebe, et al.,
bioRxiv - Bioengineering 2020
Quote:
... ROS levels in the culture were determined via Mitochondrial ROS Activity Assay Kit (eEnzyme). Briefly ...
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No products found
because this supplier's products are not listed.
Kelsey R. Moreno, et al.,
bioRxiv - Zoology 2020
Quote:
... the standard procedure of the commercial kit “PHASE”TM Haptoglobin Assay (Tridelta, Maynooth, Ireland) was followed ...
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No products found
because this supplier's products are not listed.
Mohammed Fahad Albeshr, Abdulwahed Fahad Alrefaei,
bioRxiv - Epidemiology 2019
Quote:
... The swabs were then immediately inoculated individually into InPouchTM TV culture kits (BioMed Diagnostics) using the manufacturer’s guidance and instructions ...
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No products found
because this supplier's products are not listed.
Matthew A. Lawlor, et al.,
bioRxiv - Genomics 2021
Quote:
... ribosomal RNAs were removed suing iTools rRNA depletion Kit from Galen Laboratory Supplies (dp-P020-000007) and Thermo Fisher MyOne Streptavidin C1 Dynabeads (#65001) ...
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No products found
because this supplier's products are not listed.
Enjie Li, et al.,
bioRxiv - Biochemistry 2021
Quote:
... followed by incubation with secondary antibody at room temperature and visualized using a DAB Kit (Bioworld). Then ...
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No products found
because this supplier's products are not listed.
Manal Ibrahim Kosta, et al.,
bioRxiv - Pathology 2020
Quote:
... VWF antigen was measured with a commercially available enzyme-linked immunosorbent assay kit from Diagnostica Stago.
-
No products found
because this supplier's products are not listed.
Caijun Wu, et al.,
bioRxiv - Immunology 2022
Quote:
... Mouse Factor X total antigen was measured using kit from Molecular Innovations (Cat. No. MFXKT-TOT).
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No products found
because this supplier's products are not listed.
Ayan Rajgarhia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and protein extraction was carried out using a total protein extraction Kit (BioChain Institute, Inc. Hayward, CA). Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Aarthi Subramani, et al.,
bioRxiv - Immunology 2020
Quote:
... Genomic DNA was then removed from the total RNA using a Message Clean kit (GenHunter, Nashville, TN) per the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Robert J. Fialkowski, et al.,
bioRxiv - Physiology 2022
Quote:
Oxidative DNA damage was evaluated for 8-OhDG damage using a DNA damage ELISA kit (StressMarq Biosciences Inc.) (Fialkowski et al. ...
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No products found
because this supplier's products are not listed.
Quentin Defenouillere, et al.,
bioRxiv - Cell Biology 2019
Quote:
... clone 9 and clone 10 was purified using the Qiagen genomic DNA kit (Genomic-tip 20/G) using 30 OD equivalents of material following the manufacturer’s instructions after zymolyase treatment (Seikagaku). A PCR-free library was generated from 10 µg of gDNA and sequenced at the Beijing Genomics Institute (Hong Kong ...
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No products found
because this supplier's products are not listed.
Dominic Esposito, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Transfection-quality DNA was produced in-house using the Qiagen Plasmid Plus Maxi Kit per the manufacturer’s protocols or was generated at large-scale by Aldevron (Fargo, ND).