-
No products found
because this supplier's products are not listed.
Francisco J. Calero-Cuenca, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Reverse transcription quantitative PCR (RT-qPCR) was performed using Power SYBR Green PCR MasterMix (Alfagene) according to the manufacturer’s instructions and using primers forward and reverse at 0,25 μM (final concentration ...
-
No products found
because this supplier's products are not listed.
Jinze Li, et al.,
bioRxiv - Neuroscience 2023
Quote:
Blood flow in the mouse brain was recorded in real-time using an RFLSI III device (RWD Life Sciences, Shenzhen, China) throughout the whole process of ischemic stroke ...
-
No products found
because this supplier's products are not listed.
Qiang Sun,
bioRxiv - Plant Biology 2021
Quote:
... Xylem segments were then silver-enhanced at RT for 15 min with a silver enhancement kit (BBI Solutions, UK, Batch No. 12348). After wash with DD H2O and dehydration via an ethanol series ...
-
No products found
because this supplier's products are not listed.
Brian W. Ji, et al.,
bioRxiv - Microbiology 2019
Quote:
... inverted sterile 200 µL pipette tips (Rainin RT-L200F) were used to core out a small sample from the stool ...
-
No products found
because this supplier's products are not listed.
Pradeep Ramalingam, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
-
No products found
because this supplier's products are not listed.
Maximilian Bunz, et al.,
bioRxiv - Microbiology 2023
Quote:
... or SiR-DNA dye (1 µM, 90 min, RT; Tebu-Bio). Three final washing steps were performed ...
-
No products found
because this supplier's products are not listed.
Anne-Perrine Foray, et al.,
bioRxiv - Immunology 2021
Quote:
... and an anti-IFNγ capture and detection antibody pair (U-CyTech Biosciences) were used ...
-
No products found
because this supplier's products are not listed.
Philipp Starkl, et al.,
bioRxiv - Immunology 2023
Quote:
... Primary antibodies and detection reagents used: polyclonal chicken anti-GFP (Aves labs); anti-beta-tubulin III (clone 2G7D4 ...
-
No products found
because this supplier's products are not listed.
Leanne E. Wybenga-Groot, C. Jane McGlade,
bioRxiv - Biochemistry 2020
Quote:
... 10% (w/v) PEG8000 at rt using a Mosquito robot (TTP Labtech). Small rod-like crystals were observed after approximately five months ...
-
No products found
because this supplier's products are not listed.
Qi Wang, et al.,
bioRxiv - Genomics 2023
Quote:
... and RNasin Plus (Promega)] 10-15 times using pestle A “loose” followed by pestle B “tight” 10-15 times (DWK Life Sciences, Millville, NJ, USA). Homogenate was passed through a 70 µm 1.5 ml mini strainer (PluriSelect ...
-
No products found
because this supplier's products are not listed.
Marina E. Garrett, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Vital signs were monitored with a Physiosuite (model # PS-MSTAT-RT; Kent Scientific). Eye drops (Lacri-Lube Lubricant Eye Ointment ...
-
No products found
because this supplier's products are not listed.
C Bourges, et al.,
bioRxiv - Genetics 2020
Quote:
Oligonucleotide libraries were re-suspended in nuclease-free water and amplified by emulsion PCR (NEB Q5 polymerase, 30 cycles, Micellula DNA Emulsion & Purification Kit (Chimerx)) using primers containing SfiI restriction sites ...
-
No products found
because this supplier's products are not listed.
Thomas HB FitzGerald, et al.,
bioRxiv - Neuroscience 2019
Quote:
... agents update beliefs about all states within a fixed-length time window that includes the present time but stretches a set distance into the past (Figure 1) (FitzGerald et al., 2017). This window moves forward in time at the same rate that observations are gathered ...
-
No products found
because this supplier's products are not listed.
Sanne van der Niet, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Dihydrochloride) detection was performed using anti dinitrophenol (DNP) (Polyclonal Anti-DNP, Oxford Biomedical Research). All antibodies were diluted in 1% BSA in PBS ...
-
No products found
because this supplier's products are not listed.
Zhexin Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 3 times at 10,000 rpm for 5 s (IKA TC10 basic ULTRA-TURRAX® homogenizer with S10N-5G dispersing element ...
-
No products found
because this supplier's products are not listed.
Lizhi He, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... lysed by grinding 50 times in Wheaton A (Wheaton, 357538), and then incubated on ice for 20 minutes ...
-
No products found
because this supplier's products are not listed.
Kevin Lin, et al.,
bioRxiv - Systems Biology 2023
Quote:
... two-round PCR was performed using the Cellecta NGS prep kit for sgRNA barcode libraries in pRSG16/17 (KOHGW) (Cellecta # LNGS-120) and the Supplementary Primer Sets (Cellecta #LNGS-120-SP ...
-
No products found
because this supplier's products are not listed.
Michelle E. Maxson, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Time series images were analyzed using Volocity v6.3 software (Quorum Technologies), and SidK-AL568 molecule fluorescence over time plotted to generate traces where the number of steps required for SidK-AL568 spot disappearance were counted ...
-
No products found
because this supplier's products are not listed.
Sangsu Bang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... followed by detection using an anti-GRP37L1 antibody (Bioss, Rabbit, 1:1000, Cat No. bs-15390R) or anti-flag antibody (Cell signaling ...
-
No products found
because this supplier's products are not listed.
Thibault Courtellemont, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cells were passed one time through a French press (Constant Systems LTD) at 4°C with 2.2 kpsi of pressure ...
-
No products found
because this supplier's products are not listed.
Roman Franěk, et al.,
bioRxiv - Zoology 2019
Quote:
... and 3 μl PCR H2O (Top-Bio). Reaction conditions were 30 cycles of 94 °C for 30 s ...
-
No products found
because this supplier's products are not listed.
Seesandra V. Rajagopala, et al.,
bioRxiv - Genomics 2022
Quote:
... and 7.25 μl PCR Certified Water (Teknova). RNA was reverse transcribed at 50ºC for 15 minutes ...
-
No products found
because this supplier's products are not listed.
Dena Ennis, et al.,
bioRxiv - Microbiology 2023
Quote:
... Samples were separated by HPLC with fluorescence detection on a TSKgel Amide-80 column (Tosoh Bioscience, Tokyo, Japan) with a linear gradient of a 50 mM ammonium formate/acetonitrile solvent system ...
-
No products found
because this supplier's products are not listed.
A. Schlör, et al.,
bioRxiv - Immunology 2022
Quote:
... 1 h incubation at RT) and 1-2 μg/mL of HRP-conjugated secondary antibody (ABIN1981272, antibodies-online) in 50 μL/well supplemented with PBS/1% casein or PBS/5 % NCS for 45 min incubation at RT.
-
No products found
because this supplier's products are not listed.
Michelle A. Baird, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells were incubated for 1.5 hours at RT with the following primary antibodies: LBR (1:200, Atlas Antibodies HPA062236), LaminB1 (1:500 ...
-
No products found
because this supplier's products are not listed.
Noah J Harris, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Vesicles were extruded 11 times through a 150nm NanoSizer Liposome Extruder (T&T Scientific) and stored at −80°C.
-
No products found
because this supplier's products are not listed.
Marianne E Emmert, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... through detection of the 7-amino-4-methylcoumarin (AMC) labeled fluorogenic peptide substrates Z-LLE-AMC (Boston Biochem #S-230) and LLVY-AMC (Chemicon #APT280) ...
-
No products found
because this supplier's products are not listed.
Jingyao Li, et al.,
bioRxiv - Genomics 2021
Quote:
... 10 nl of 1 uM barcoded DRUG-seq RT primers were then dispensed into each well using an Echo 555 Liquid Handler (Labcyte). Plates were sealed (BioRad ...
-
No products found
because this supplier's products are not listed.
Rémi Porte, et al.,
bioRxiv - Immunology 2024
Quote:
... brain cell suspensions were incubated for 45 min at RT with the following barcoded dextramers diluted in complete RPMI: Kb-SIINFEKL (Immudex cat # JD2163-PfBC ...
-
No products found
because this supplier's products are not listed.
John Paul Tsu Ouyang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Samples were then shaken at 1500 RPM for five minutes at RT in a benchtop shaker (Benchmark Scientific; Model #: H5000-HC). Samples were then incubated for 5 minutes at RT with no shaking ...
-
No products found
because this supplier's products are not listed.
Clara Sidor, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... was amplified by PCR from the mNeonGreen vector (Allele Biotechnology), with the exclusion of the stop codon and the addition of a C-terminal linker.
-
No products found
because this supplier's products are not listed.
Ryan Finethy, et al.,
bioRxiv - Immunology 2020
Quote:
... and cells were blocked with PBS containing 5% Bovine Serum Albumin (BSA) for 30 min at RT Cells were stained with rabbit anti-ASC antibody (AG-25B-0006; Adipogen; 1:1000) diluted in blocking buffer overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... resuspended proteins were PEGylated for 1 hr at RT to label newly exposed cysteine thiols with the 5 kDa mass tag reagent (mPEG-5k, Badrilla SiteCounter™). Approximately 20 μl of each supernatant was saved as the “total input.” For immunoblot analysis ...
-
No products found
because this supplier's products are not listed.
Xingjie Ren, et al.,
bioRxiv - Genomics 2023
Quote:
... we transfected two million MCF7-dCas9/RT cells with 2000 ng of ngRNA/pegRNA plasmid containing an EGFP marker using PolyJet (SignaGen Laboratories, SL100688). Five days after transfection ...
-
No products found
because this supplier's products are not listed.
Aaron Lanz, Grant Gordon, Jaideep Bains,
bioRxiv - Neuroscience 2020
Quote:
... Virus was pressure injected into the brain three times with a Nanoject II apparatus (Drummond Scientific Company), each injection at a volume of 69 nL and placed increasingly deeper into the brain (AP ...
-
No products found
because this supplier's products are not listed.
Cody Juguilon, et al.,
bioRxiv - Physiology 2021
Quote:
... The beads with EC were washed several times and cultured in mouse EC cultured medium (Cell Biologics). When the cells were confluent ...
-
No products found
because this supplier's products are not listed.
Leonardo Betancurt-Anzola, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The complex was incubated at RT for 20 mins and pipetted onto glowed discharged cryo-EM grids (Quantifoil R 2/2 Au 300). Grids were frozen in liquid ethane by using a Vitrobot Mark IV (ThermoFischer ...
-
No products found
because this supplier's products are not listed.
Robert R. Bowers, et al.,
bioRxiv - Genetics 2021
Quote:
... coli kit (MS-CRED-KIT) was purchased from Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Eva Jarc Jovičić, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The retention time and at least six diagnostic ions for each lipid mediator were confirmed by means of external standards (Cayman Chemical/Biomol GmbH). Quantification was achieved by calibration curves for each lipid mediator ...
-
No products found
because this supplier's products are not listed.
Elisabet Perez-Coronel, J. Michael Beman,
bioRxiv - Genomics 2021
Quote:
... Temperature and dissolved oxygen were measured at the time of sample collection using a ProODO YSI probe (YSI Inc., Yellow Springs, OH, USA).
-
No products found
because this supplier's products are not listed.
Mindy Engevik, et al.,
bioRxiv - Cell Biology 2022
Quote:
Albumin concentration in stool was determined using a commercial kit (Albumin ELISA Kit, Genway, GWB-282C17) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Eric T. Son, et al.,
bioRxiv - Immunology 2021
Quote:
... QuickSwitch Custom Tetramer Kits (MBL International) were utilised to generate multiple tetramers with selected peptides in order to screen an array of pMHC epitopes ...
-
No products found
because this supplier's products are not listed.
Chengyan Wu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The CCK8 kit was purchased from Abbkine. The reactive oxygen detection kit for tissue sections was purchased from Shanghai Pebble Biotechnology Company ...
-
No products found
because this supplier's products are not listed.
Wyatt G. Paltzer, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Aniline Blue Stain Kit” (Newcomer Supply, #9179B) following the kit protocol ...
-
No products found
because this supplier's products are not listed.
Luca Valzania, Aya Alami, Pierre Léopold,
bioRxiv - Developmental Biology 2023
Quote:
... using the 20-Hydroxyecdysone ELISA kit (Bertin bioreagent, #A05120) as recommended by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Ichiro Yamauchi, et al.,
bioRxiv - Physiology 2023
Quote:
... TSH AccuLite VAST CLIA kit (Monobind Inc., Lake Forest, CA). We diluted serum samples 25-fold with phosphate buffered saline when we measured human TSH levels ...
-
No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... I-FABP (Human I-FABP ELISA Kit, Hycult biotech, Cat# HK406), and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit ...
-
No products found
because this supplier's products are not listed.
Zhouyi Rong, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Mouse Interferon Gamma (IFNg) ELISA Kit (RD-IFNg-Mu, Reddot biotech), Mouse Interleukin 6 (IL6 ...
-
No products found
because this supplier's products are not listed.
Thomas Klein, et al.,
bioRxiv - Neuroscience 2023
Quote:
... iPSC were generated using the StemRNA 3rd Gen reprogramming kit (Reprocell, Maryland, USA) for all male cell lines (FD-1 ...
-
No products found
because this supplier's products are not listed.
Caroline Passaes, et al.,
bioRxiv - Immunology 2019
Quote:
... Culture supernatants were assayed on day 7 using an SIV p27 Antigen ELISA Kit (Zeptometrix). Antiviral activity was calculated as log10 (mean p27 ng/mL in SIV-infected CD4+ T-cell cultures without CD8+ T-cells ...