-
No products found
because this supplier's products are not listed.
Petra Hafker, et al.,
bioRxiv - Physiology 2021
Quote:
... we used a benchtop chiller with an antifreeze bath set to 1°C (MicroCooler II Model 260010, Boekel Scientific ...
-
No products found
because this supplier's products are not listed.
Allison K. Meyers, et al.,
bioRxiv - Immunology 2021
Quote:
... peripheral white blood cells were stained with FLICA 660-YVAD-FMK (1:150 dilution, ImmunoChemistry Technologies), CD115-PE (eBioscience ...
-
No products found
because this supplier's products are not listed.
Shabirul Haque, et al.,
bioRxiv - Immunology 2023
Quote:
... split into two eppendorf tubes prefilled with 150 μl of 0.5-mm glass beads (Scientific Industries) in each tube ...
-
No products found
because this supplier's products are not listed.
Jiling Feng, Yuexun Tang, Wenwei Fu, Hongxi Xu,
bioRxiv - Cell Biology 2023
Quote:
... RT-PCR was performed with a one-step real time PCR using KAPA SYBR FAST One-Step qRT-PCR Universal (D-MARK Biosciences). Primers were used as previously described [18] ...
-
No products found
because this supplier's products are not listed.
Serdar Turkarslan, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
Ancestor and EPD cocultures were revived anaerobically in 18×150-mm balch tubes (Chemglass Life Sciences: CLS420901) from freezer stocks through dilution into CCMA media to ensure syntrophic growth and prevent carryover of glycerol into the fresh growth medium ...
-
No products found
because this supplier's products are not listed.
Tsunaki Hongu, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... mice were intravenously injected with clodronate-liposome (100-150 μl per mouse, every other day, Liposoma BV). Short-term experiments were performed by treatment with clodronate-liposome on day 14 ...
-
No products found
because this supplier's products are not listed.
Tasneem Qaqorh, et al.,
bioRxiv - Cell Biology 2023
Quote:
... followed by in situ mRNA reverse transcription using photo-caged oligodeoxynucleotides (ODNs) primers (Glen research). After first strand synthesis ...
-
No products found
because this supplier's products are not listed.
Katie N. Kang, Joseph M. Boll,
bioRxiv - Microbiology 2022
Quote:
... overnight cultures were back diluted to OD600 0.01 and set up as triplicate biological replicas in either a 96-well plate or 24-well plate (BrandTech BRAND). A BioTek SynergyNeo2 microplate reader was used to record optical density ...
-
No products found
because this supplier's products are not listed.
Ana Cascalho, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and incubated with horseradish peroxidase conjugated anti-rabbit antibody diluted in blocking buffer (Jackson Immunoreagents: 715-035-150). They then received a final series of washing steps ...
-
No products found
because this supplier's products are not listed.
Christopher D. Go, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Beads were then washed with 150 μl of HPLC-grade water (Caledon Laboratory Chemicals CAT# 7732-18-5), centrifuged at 400 RCF for 1 min to pellet beads ...
-
No products found
because this supplier's products are not listed.
Zsuzsa Radvanyi, et al.,
bioRxiv - Physiology 2023
Quote:
... The resultant concentrate (150 µl) was used to measure c-terminal FGF23 by ELISA (Quidel; Cat. #60-6300).
-
No products found
because this supplier's products are not listed.
Jan Zlamal, et al.,
bioRxiv - Immunology 2022
Quote:
... a calcium independent ligand of PS (200nM, 15 min, RT; Haematologic Technologies, Essex Junction, USA).29,30 Sufficient blocking concentrations of Lactadherin were assessed via Calibrated Automated Thrombogram (CAT ...
-
No products found
because this supplier's products are not listed.
R. Doolan, et al.,
bioRxiv - Immunology 2024
Quote:
... and reverse transcribed using FIREScript RT cDNA synthesis Kit Solis Biodyne (Lucerna 06 −15-00200) for qPCR analysis ...
-
No products found
because this supplier's products are not listed.
Jarrod C. Harman, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Mice were thermoregulated at a body temperature of 37±0.5 °C throughout the procedure using a temp-set YSI 402 micro temperature controller (Yellow Springs Instruments) system with a rectal probe reference ...
-
No products found
because this supplier's products are not listed.
Joana Saldida, et al.,
bioRxiv - Systems Biology 2020
Quote:
... The third set of cultures contained a mixture of 80% unlabelled and 20% uniformly labelled [U-13C] glucose (Buchem, CLM-1396). From each flask ...
-
No products found
because this supplier's products are not listed.
Kohitij Kar, James J DiCarlo,
bioRxiv - Neuroscience 2020
Quote:
... we used an in-house set up to lower the injection needles (30-32 gauge, small Hub RN Needle; Hamilton Company) using a micro-syringe pump and controller (Micro4™ World Precision Instruments) ...
-
No products found
because this supplier's products are not listed.
Tetsuhiro Harimoto, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... 150 μL of blood from the single donor human whole blood or murine (BALB/c) whole blood (Innovative Research) were aliquoted into 3 wells/strain in a 96-well plate ...
-
No products found
because this supplier's products are not listed.
Thomas A. Desautels, et al.,
bioRxiv - Microbiology 2023
Quote:
Immunoassays for screening the first set of 230 designs (Fig. ED1) and later characterization were performed on the Gyrolab xPlore instrument (Gyros Protein Technologies) using the Bioaffy 200 discs (Gyros Protein Technologies) ...
-
No products found
because this supplier's products are not listed.
Giuditta Annibaldis, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... with or without knockdown (two 150-mm culture dishes each condition) were treated with 100 µg/ml cycloheximide (Focus biomolecules) for 4 min at 37 °C ...
-
No products found
because this supplier's products are not listed.
Qian Zhou, et al.,
bioRxiv - Neuroscience 2021
Quote:
We delivered ∼150 nl of AAV virus with a stereotaxic instrument (David Kopf Instruments, #PF-3983; RWD Life Science, #68030) and a pressure micro-injector (Nanoject II ...
-
No products found
because this supplier's products are not listed.
Sarah Vogt, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 25 μg of Ta-sro1 WWE-PARP was mixed with 175 μL SEC buffer and 150 μL 10 μM PAR (Trevigen). The control sample consisted of 25 μg of Ta-sro1 WWE-PARP and 325 μL SEC buffer.
-
No products found
because this supplier's products are not listed.
Adam G. Maynard, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... and resuspended in 150 μl of porphyrin extraction buffer (1:4 ratio of 1.7 M HCl:ACN, 1μM deuteroporphyrin IX (Frontier Scientific, D510-9)) and 0.5 μM isotopically labeled amino acids (Cambridge Isotopes ...
-
No products found
because this supplier's products are not listed.
Thomas HB FitzGerald, et al.,
bioRxiv - Neuroscience 2019
Quote:
... agents update beliefs about all states within a fixed-length time window that includes the present time but stretches a set distance into the past (Figure 1) (FitzGerald et al., 2017). This window moves forward in time at the same rate that observations are gathered ...
-
No products found
because this supplier's products are not listed.
Richard B. Kennedy, et al.,
bioRxiv - Immunology 2020
Quote:
IFNα and IFNλ production by 2’3’ cGAMP-treated STING-transduced BJAB cells were measured in triplicate using commercial sandwich ELISA assay sets (IFNα: VeriKine-HS™ Human Interferon Alpha All Subtype ELISA Kit, PBL Assay Science; Piscataway ...
-
No products found
because this supplier's products are not listed.
Dapeng Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... SMG) and the normal gravity control (NG) were set up in a high-aspect rotating vessel (HARV) bioreactor (Synthecon, Inc., Houston, Texas, USA) and cultured continuously ...
-
No products found
because this supplier's products are not listed.
Etkin Akar, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... then long-term drug responses were recorded every 30 minutes through (RT-CES) (ACEA Biosciences; Agilent Technologies). Cell Index values (CI ...
-
No products found
because this supplier's products are not listed.
Minsuk Kong, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... Increasing ratios (multiplicities of incubation ranging from 20 to 150) of SSHELαHER2 (labeled with AlexaFluor647) were incubated with 5 × 105 cells/ml in siliconized tubes (G-tubes, Bio Plas Inc.) for 1 h at 37 °C with gentle inversion ...
-
No products found
because this supplier's products are not listed.
Atsuhiko Ichimura, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and embedded in Epon to prepare thin sections (100∼150 nm thickness) for analysis under a transmission electron microscope (JEM-200CX, JEOL, Japan).
-
No products found
because this supplier's products are not listed.
Jens O. Watzlawik, et al.,
bioRxiv - Neuroscience 2024
Quote:
HEK293E cells and MEFs were lysed in RIPA buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 1% NP-40 [United States Biological, 19628] ...
-
No products found
because this supplier's products are not listed.
A. Schlör, et al.,
bioRxiv - Immunology 2022
Quote:
... 1 h incubation at RT) and 1-2 μg/mL of HRP-conjugated secondary antibody (ABIN1981272, antibodies-online) in 50 μL/well supplemented with PBS/1% casein or PBS/5 % NCS for 45 min incubation at RT.
-
No products found
because this supplier's products are not listed.
Joanna G. Rothwell, et al.,
bioRxiv - Microbiology 2021
Quote:
... The body of the DBDD reactor consisted of a 150 mm long gas dispersion tube with a fritted glass tip with a pore size 10-20 μm (Ace Glass 720208). A sealed borosilicate tube (≈4mm OD ...
-
No products found
because this supplier's products are not listed.
Thanh-Tam Tran, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Immunohistochemistry to detect the expression of APOA1BP in the gingiva was performed using primary antibodies rabbit anti-APOA1BP (Biorbyt, orb155691; 1:150). The stained sections were analyzed under a Zeiss Axio Scope A1 microscope.
-
No products found
because this supplier's products are not listed.
Christopher Wong, Elena M. Jurczak, Richard Roy,
bioRxiv - Developmental Biology 2023
Quote:
... The gene expression levels were determined by RT-qPCR using a 2x SyberGreen qPCR Master-mix (ZmTech Scientifique, Q2100N) and a Bio-Rad CFX384 Real-Time 96-well PCR qPCR Detection System (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Sandrine Valade, et al.,
bioRxiv - Immunology 2021
Quote:
... ADAMTS13 activity (N: 50-150 IU/dL) was measured with in-house FRETS-VWF73 assay using the recombinant VWF73 peptide (Peptide Institute, Osaka, Japan), as previously described [29] ...
-
No products found
because this supplier's products are not listed.
Azaz Ahmed, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Langerhans islets cells were placed in a 96-well plate (around 7500 cells/ per well) and cultured in a mixture of 150 μl volume of complete growth medium with serum (Celprogen, USA; M35002-04S) and 50 μl of supernatant of human PDA tissue explants ...
-
No products found
because this supplier's products are not listed.
Hayley R. Waterman, et al.,
bioRxiv - Immunology 2023
Quote:
25,000 – 45,000 pDCs were added to 96 well U bottom plate wells in 150 μl of culture medium (RPMI with 2% heat inactivated human AB serum (Omega Scientific HS-20), 20 ng/mL IL-3 (Peprotech 200-03) ...
-
No products found
because this supplier's products are not listed.
Kristin S. Fitzpatrick, et al.,
bioRxiv - Immunology 2022
Quote:
... into empty 96-well low profile PCR plates (Labcon), which were sealed with PCR microplate sealing foil (Eppendorf) ...
-
No products found
because this supplier's products are not listed.
Emily EF Fekete, et al.,
bioRxiv - Systems Biology 2024
Quote:
... Peptides were loaded onto a tip column (75 μm diameter, 150 mm length) packed with reverse phase beads (3 μm/120 Å ReproSil-Pur C18 resin, Dr. Maisch HPLC GmbH). A 60 min gradient of 5 to 35% (v/v ...
-
No products found
because this supplier's products are not listed.
A Elgheznawy, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Platelets were activated with appropriately diluted agonists for 8 min at 37°C followed by 8 min at RT in the presence of saturating amounts of PE-coupled JON/A (4H5, Emfret Analytics) detecting activated αIIbβ3 integrin and FITC-coupled anti-P-selectin (Wug.E9 ...
-
No products found
because this supplier's products are not listed.
Johanna Hol Fosse, et al.,
bioRxiv - Microbiology 2023
Quote:
... Acetone-fixed cells were incubated with IgG1 against the ISAV nucleoprotein (P10, Aquatic Diagnostics Ltd, 0.4 μg/mL, 60 min, RT), washed (PBS × 3) ...
-
No products found
because this supplier's products are not listed.
Linda M. Sircy, et al.,
bioRxiv - Immunology 2023
Quote:
... virus strain for 30min at room temperature (RT) prior to addition of 50μL of 1% chicken red blood cells (cRBCs) (Lampire Biological Laboratories) in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Vipul Sharma, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... amplified using Taq Polymerase 2X PCR premix (Intact Genomics, 3249) and SX primers (SX_F ...
-
No products found
because this supplier's products are not listed.
Rishi R. Agrawal, et al.,
bioRxiv - Neuroscience 2022
Quote:
... the slides were equilibrated at RT for 25 min and stained using the Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit (Biosensis TR-100) following manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Max F. Moescheid, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 10 µM of 5’amine C6 primers (IDT, Coralville, IA) were incubated with 1 mM Bis-PEG10-NHS ester (BroadPharm, San Diego, CA) in borate buffer (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Timothy M. Ghaly, et al.,
bioRxiv - Microbiology 2021
Quote:
... All PCRs were performed using GeneReleaser® (Bioventures, Murfreesboro, TN, USA) as previously described [32] ...
-
No products found
because this supplier's products are not listed.
Emily M. Mace, et al.,
bioRxiv - Immunology 2019
Quote:
... selected with puromycin and confirmed by PCR for SV40 expression (Applied Stemcell). 293T fibroblast lines were maintained in DMEM supplemented with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Nora Hartig, Kathrin M. Seibt, Tony Heitkam,
bioRxiv - Plant Biology 2022
Quote:
... PCR products were purified (As One International,Invisorb Spin DNA Extraction Kit) and ligation into pGEM-T plasmids (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Jennifer Hampton Hill, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... all contain 6X-His tags and were incubated with individual membrane strips or arrays at a final concentration of 0.75 μg/mL for 1 hr at RT as directed by the manufacturer (Echelon Biosciences, Inc., Salt Lake City, UT). For visualization ...
-
No products found
because this supplier's products are not listed.
Paul Mollenkopf, et al.,
bioRxiv - Biophysics 2021
Quote:
... These strands were hybridized in a Professional Standard PCR Thermocycler (Core Life Sciences Inc., USA) by denaturation for 10min at 90°C and complementary base pairing for 20h between 80° C and 20° C ...
-
No products found
because this supplier's products are not listed.
Drake Jensen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... These templates underwent an additional PCR step with Klentaq LA DNA Polymerase (84) (DNA Polymerase Technology; catalog # 110), using a biotinylated primer (Integrated DNA Technologies ...