-
No products found
because this supplier's products are not listed.
Joel D. Pearson, et al.,
bioRxiv - Microbiology 2020
Quote:
The 2019-nCoV TaqMan RT-PCR Kit from Norgen Biotek and 2019-nCoV ...
-
No products found
because this supplier's products are not listed.
Francisco J. Calero-Cuenca, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Reverse transcription quantitative PCR (RT-qPCR) was performed using Power SYBR Green PCR MasterMix (Alfagene) according to the manufacturer’s instructions and using primers forward and reverse at 0,25 μM (final concentration ...
-
No products found
because this supplier's products are not listed.
William N. Voss, et al.,
bioRxiv - Immunology 2024
Quote:
... resuspended in a one-step RT-PCR solution with an overlap extension VH and VL primer set as previously described,6 emulsified using a dispersion tube (IKA), and subjected to overlap-extension RT-PCR under the following conditions ...
-
No products found
because this supplier's products are not listed.
Ryota Kurimoto, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... We also synthesized RI-labeled pri-let-7a1 in which pseudouridine was randomly introduced using UTP: pseudouridine (Carbosynth) at a ratio of 1:1 ...
-
No products found
because this supplier's products are not listed.
Giovannino Silvestri, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... anti-SET (Globozymes); anti-ABL (Ab-3) ...
-
No products found
because this supplier's products are not listed.
Susan Paton, et al.,
bioRxiv - Microbiology 2021
Quote:
... RT-PCR was performed using the VIASURE SARS-CoV-2 Real Time PCR Detection Kit (Viasure; CerTest Biotec, Zaragoza, Spain), following the methods provided ...
-
No products found
because this supplier's products are not listed.
Jun Cao, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... three sets of E20 and three sets of 6M rat heart RNA (Zyagen) were used ...
-
No products found
because this supplier's products are not listed.
Joseph H. Chapman, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The plate was sealed with Avant ThermalSeal optically clear polyester RT-PCR film (MIDSCI TS-RT2-100), briefly spun down ...
-
No products found
because this supplier's products are not listed.
Nathan Singh, et al.,
bioRxiv - Immunology 2019
Quote:
... and perforin (antibody sets from MABTECH), using ELISA substrate ADHP from Cayman Chemical ...
-
No products found
because this supplier's products are not listed.
Sadis Matalon, et al.,
bioRxiv - Physiology 2023
Quote:
... The 8.1kb mtDNA was amplified by PCR and quantitatively analyzed by real-time PCR using specific primers provided in the Mouse Real-Time PCR Mitochondrial DNA Damage Analysis Kit from Detroit R&D (cat. #DD2M; Detroit, MI). Mitochondrial damage in the 8.1kb mtDNA fragment was quantified by a standard curve prepared ...
-
No products found
because this supplier's products are not listed.
Preeti Verma, et al.,
bioRxiv - Biophysics 2024
Quote:
... another set of reaction was set up wherein 5U of endo-(1,4)-β-glucanase (E-CELTR; Megazyme) was added at the beginning of the synthesis reaction for the enzymatic degradations of the in vitro synthesized glucan ...
-
No products found
because this supplier's products are not listed.
Aisha A. AlJanahi, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... supplemented with 1% HSA (Baxter) and cytokines (SCF 100ng/mL, FLT3L 100ng/mL and TPO 100ng/mL; all from PeproTech)55 ...
-
No products found
because this supplier's products are not listed.
Nicole M. Gaudelli, et al.,
bioRxiv - Genomics 2020
Quote:
... the cells were spun down to remove the X-VIVO 10 media and washed in MaxCyte buffer (HyClone) with 0.1% HSA (Akron Biotechnologies). The cells were then resuspended in cold MaxCyte buffer at 1,250,000 cell per mL and split into multiple 20µL aliquots ...
-
No products found
because this supplier's products are not listed.
Iuliia Polina, et al.,
bioRxiv - Cell Biology 2023
Quote:
... containing 0.35% (w/v) human serum albumin (HSA) 0.35% (w/v), and prostaglandin I2 (PGI2, 0.5 μM) (Cayman Chemical, Ann Arbor, Michigan). Platelets were immobilized on the glass bottom plates (Cellvis Mountain View ...
-
No products found
because this supplier's products are not listed.
Jimin Yoon, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Cells were grown to confluency and then screened based on functional testing of the XBP1s construct using RT-PCR (described below) with or without 2 μg/mL doxycycline (dox; Alfa Aesar). The selected SupT1 single colony cell line encoding tetracycline-inducible XBP1s was then transduced with lentivirus encoding DHFR.ATF6(1–373 ...
-
No products found
because this supplier's products are not listed.
Markus Hackl, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The NTA modified primer (backward primer, 5’-NTA-SS-C6-TCCAAAGGTGAAGAACTGTTCACC) was purchased from Gene Link, Inc ...
-
No products found
because this supplier's products are not listed.
Xanthe A.M.H. van Dierendonck, et al.,
bioRxiv - Physiology 2019
Quote:
... NEFAs (NEFA-HR set R1, R2 and standard, WAKO Diagnostics, Instruchemie ...
-
No products found
because this supplier's products are not listed.
Mikael Lundqvist, et al.,
bioRxiv - Neuroscience 2022
Quote:
... a new set of acute electrode pairs (tungsten, epoxy-coated, FHC) was lowered through a grid ...
-
No products found
because this supplier's products are not listed.
Dolunay Kelle, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... a total reaction volume of 20 µl was prepared consisting of either 2 µl of telomere primers or 2 µl of SCR primers from the Relative Human Telomere Length Quantification qPCR kit (ScienCell), 1 µl of genomic DNA template (5 ng/µl) ...
-
No products found
because this supplier's products are not listed.
Sara C. Di Rienzi, et al.,
bioRxiv - Microbiology 2022
Quote:
... set in a size 0 silicone cork (7752-15, Cole-Parmer, USA) in a 20 × 125 mm glass reactor tube (9826 ...
-
No products found
because this supplier's products are not listed.
Joseph L. Basalla, et al.,
bioRxiv - Biochemistry 2023
Quote:
Samples for imaging were set up in 16 well CultureWells (Grace BioLabs). Wells were passivated by overnight incubation in 5% (w/v ...
-
No products found
because this supplier's products are not listed.
Joseph L. Basalla, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Samples were set up in 96 well glass-bottom plates (Thomas Scientific) and absorbance at 350 nm was measured as previously described (15) ...
-
No products found
because this supplier's products are not listed.
Sean K. Ryan, et al.,
bioRxiv - Pathology 2019
Quote:
... RT cocktail consists of: 50mM Tris (Amresco J837) pH 7.8 ...
-
No products found
because this supplier's products are not listed.
Molly Brady, et al.,
bioRxiv - Neuroscience 2021
Quote:
... a NIRF filter set (Semrock ICG-B, IDEX Health & Science LLC Rochester NY) and camera (Prosilica GT1380 ...
-
No products found
because this supplier's products are not listed.
Christelle Damon-Soubeyrand, et al.,
bioRxiv - Developmental Biology 2022
Quote:
Ultramicroscopy data sets were uploaded to IMARIS version 9.7 (Bitplane, Oxford Instruments England). The stacks were converted to IMARIS files (.ims ...
-
No products found
because this supplier's products are not listed.
François Mallard, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... and took the set maximizing the difference in median Integrated Complete-data Likelihood (ICL) between control groups (log area ...
-
No products found
because this supplier's products are not listed.
Laurin Heinrich, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and segmented using the CODEX Processor set at default manufacturer values (Akoya Biosciences, 2021b). Final data were then viewed using the CODEX Multiplex Analysis Viewer (MAV ...
-
No products found
because this supplier's products are not listed.
Mahmudul Hasan, et al.,
bioRxiv - Microbiology 2021
Quote:
... Sitting drop crystallization trials were set up with screens Wizard I and II (Rigaku), PEG/Ion (Hampton Research) ...
-
No products found
because this supplier's products are not listed.
Isabella Joubert, et al.,
bioRxiv - Immunology 2019
Quote:
... cell fixation and permeabilization was performed using the FoxP3 Staining Buffer Sets (Tonbo Biosciences) according to the manufacturer’s instructions and 10% naïve mouse serum (in permeabilization buffer ...
-
No products found
because this supplier's products are not listed.
George C. Russell, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Purified RNA was treated with RTS DNase (Cambio, Cambridge, UK) before cDNA synthesis.
-
No products found
because this supplier's products are not listed.
Logan K. Smith, Kareem Fawaz, Bebhinn Treanor,
bioRxiv - Immunology 2020
Quote:
... Fluorescence intensity was converted into molecular count using a fluorescence standard38 (Quantitation set, Bangs Laboratories).
-
No products found
because this supplier's products are not listed.
Jose Sandoval, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... and loaded onto sets of Octet RED 384 SSA biosensors (Pall ForteBio LLC., Menlo Park, CA) to density of 12 nm followed by blocking of non-occupied streptavidin residues of biosensors with 200 u biotin ...
-
No products found
because this supplier's products are not listed.
Martina de Majo, et al.,
bioRxiv - Neuroscience 2022
Quote:
... One set of GRN−/− astrocytes was treated with PGRN 1 μg/ml (Adipogen, AG-40A-0188Y) every 72 hours ...
-
No products found
because this supplier's products are not listed.
Johanna Hol Fosse, et al.,
bioRxiv - Microbiology 2023
Quote:
... treated with blocking buffer (Background sniper, Biocare Medical, 30 min, RT), incubated with mouse IgG1 specific to ISAV HE (clone 3H6F8 [52] ...
-
No products found
because this supplier's products are not listed.
Gen Honda, et al.,
bioRxiv - Biophysics 2020
Quote:
... Prepared SU-8 and glass substrates were set at the bottom of φ35 mm culture dish (MatTek) using a 9 × 9 mm2 frame seal (SLF0201 ...
-
No products found
because this supplier's products are not listed.
Marina E. Garrett, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Vital signs were monitored with a Physiosuite (model # PS-MSTAT-RT; Kent Scientific). Eye drops (Lacri-Lube Lubricant Eye Ointment ...
-
No products found
because this supplier's products are not listed.
Olga Gewartowska, et al.,
bioRxiv - Genetics 2020
Quote:
... and secondary goat anti-rabbit HRP conjugated antibody (Agrisera, AS101069; 1 h, RT) was performed in antibody dilution buffer (1% BSA ...
-
No products found
because this supplier's products are not listed.
Valeria Scagliotti, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... samples were incubated at room temperature (RT) with Histoclear II (National Diagnostics, HS202) (2 x 20 minutes for E9.5-E11.5 and postnatal pituitaries ...
-
No products found
because this supplier's products are not listed.
Daniel Nichol, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Sequencing of the SHV gene was performed using M13 primers (MCLab, Harbor Way, CA).
-
No products found
because this supplier's products are not listed.
Kie Kumaishi, et al.,
bioRxiv - Plant Biology 2021
Quote:
... and 1 μM blocking primers (mPNA and pPNA, PNA BIO, Inc., Newbury Park, CA). PCR was performed using the following specifications ...
-
No products found
because this supplier's products are not listed.
Gary Reynolds, et al.,
bioRxiv - Immunology 2020
Quote:
... cells were fixed for 30 minutes at RT in a solution of 0.5x Fix Buffer (OXP3 buffer set, eBiosciences, 00-5523-00) supplemented with 1.6% formaldehyde (Polysciences, 18814). Post-fixation ...
-
No products found
because this supplier's products are not listed.
Elizaveta O. Boldinova, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Primer-18 was 5′-labeled with [γ-32P]-ATP by T4 polynucleotide kinase (SibEnzyme, Russia) and annealed to the corresponding unlabeled Template-55 at a molar ratio of 1:1.1 ...
-
No products found
because this supplier's products are not listed.
Diana Legutko, et al.,
bioRxiv - Neuroscience 2023
Quote:
... was set to 720 nm and used to uncage 4-Methoxy-7-nitroindolinyl-caged-L-glutamate (MNI-caged glutamate, Tocris). Up to four spines in separate regions of interest (ROI ...
-
No products found
because this supplier's products are not listed.
Roman Franěk, et al.,
bioRxiv - Zoology 2019
Quote:
... and 3 μl PCR H2O (Top-Bio). Reaction conditions were 30 cycles of 94 °C for 30 s ...
-
No products found
because this supplier's products are not listed.
Seesandra V. Rajagopala, et al.,
bioRxiv - Genomics 2022
Quote:
... and 7.25 μl PCR Certified Water (Teknova). RNA was reverse transcribed at 50ºC for 15 minutes ...
-
No products found
because this supplier's products are not listed.
Jingyu Diao, et al.,
bioRxiv - Microbiology 2020
Quote:
... The reaction was quenched after 60 minutes at RT with 0.5 μL of 4.8% Lauryl Dimethylamine-N-Oxide (Anatrace), followed by addition of 6 μL Detection Solution ...
-
No products found
because this supplier's products are not listed.
Sara Meril, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 10μM forward and reverse primers (see Table 1) and 5μL of AzuraView GreenFast qPCR Blue Mix LR (Azura Genomics, AZ2305). Data was analyzed using QuantStudio 5 software (Thermo-Scientific) ...
-
No products found
because this supplier's products are not listed.
Robert Hardt, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Blots were washed thrice with TBS-T and incubated with one of the following secondary antibodies for 1 h at RT: HRP-goat anti-rabbit (1: 5,000, goat pAb, 111-035-003, Dianova, Germany) or HRP-goat anti-mouse (1:5,000 ...
-
No products found
because this supplier's products are not listed.
Clara Sidor, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... was amplified by PCR from the mNeonGreen vector (Allele Biotechnology), with the exclusion of the stop codon and the addition of a C-terminal linker.
-
No products found
because this supplier's products are not listed.
Steven A Kemp, et al.,
bioRxiv - Microbiology 2021
Quote:
... 9 DNA fragments with overlap sequences were amplified by PCR from a plasmid (phCMV1, Genlantis) encoding the full-length SARS-CoV-2 S gene (BetaCoV/Wuhan-Hu-1/2019 ...