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hsa let 7f RT PCR Primer Set
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Cohesion Biosciences Sponsored
Stem-loop RT primer and PCR primer set to detect mature miRNA hsa-let-7f expression.Cat# CPK3006, 1 nmol USD $100.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Nature Communications doi: 10.1038/s41467-018-06526-1Quote: ... hsa-let-7a-5p (Ambion, Cat. No# PM10050), hsa-miR-320a-3p (Ambion ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... commercially obtained hsa-let-7a-5p or hsa-miR-205-5p miRCURY LNA miRNA Inhibitor (Qiagen, cat# YI04101776 and YI04101508 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2021Quote: ... Standard RT-PCR using independent sets of Predesigned KiCqStart® SYBR® Green Primers (Sigma, Table S11) was carried out for validation with independent biological replicates from 2D cultures (n = 2 ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2018Quote: ... Prime PCR primer sets were purchased from Bio-Rad. Expression results were calculated by ΔΔCT method and were normalized to the reference genes GAPDH and βActin. -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... F1 dumpy animals were isolated and genotyped by PCR with let-7_SEQ_F5/R5 primers followed by an analysis of the PCR product using restriction digestion using EcoRV (NEB, Cat:N3195) and Sanger sequencing as described in (Duan et al. ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019, published in npj Systems Biology and Applications doi: 10.1038/s41540-019-0121-4Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Disease Models & Mechanisms doi: 10.1242/dmm.031716Quote: ... RT-PCR performed using specific primers sets (Supplementary Tables 2, 3) and GoTaq® Green Master Mix (Promega, Madison, WI). (30-40 cycles ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2022Quote: ... Primer sets were designed using ProbeFinder (Roche) and Primer 3 softwares and can be seen as Supporting Information (Table S1) ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma Plus PCR Primer Set (Agilent) was used to determine mycoplasma for all cell lines ... -
American BioInnovations,
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... TaqMan miRNA stem-loop RT primers/qPCR primer probe sets (ABI 4427975) (cel-miR-39 ID# 000200 Lot#P180110-003B10 ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were set up in a 384-well format using 2X SensiFAST Probe No-ROX Kit (Bioline, BIO-86005) and 1 µl of cDNA per reaction in a total volume of 10 µl ... -
Lucigen
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019, published in npj Systems Biology and Applications doi: 10.1038/s41540-019-0121-4Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... a region bearing six let-7 binding sites was PCR-amplified from MSCV puro let-7 sponge (Addgene #29766) and cloned between AgeI and EcoRI sites downstream of GFP. -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ... -
Eurogentec
No products found because this supplier's products are not listed.Cited in FANCD2 tunes the UPR preventing mitochondrial stress-induced common fragile site instabilitybioRxiv - Cell Biology 2019Quote: ... PCR primers were purchased from Eurogentec and used in PCRs with SYBR Green Master Mix (ThermoFisher ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... RT PCR was performed using the RealPlex2 (Eppendorf). Expression was normalized to ß-Actin (Mm02619580_g1 ... -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... The primer sets used were Quick-16S™ Primer Set V3-V4 (Zymo Research). The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation ... -
Research Products International
No products found because this supplier's products are not listed.bioRxiv - Genomics 2018Quote: ... separate PCR reactions were set up to generate the ADT library (SI-PCR and RPI-x primers), the hashtag library (SI-PCR and D7xx_s ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... FITC-CD24 (HSA, M1/69, BD Biosciences), and the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... Reactivation of the integrated HIV reporter construct was then assessed by staining for murine HSA with an anti-HSA-PE antibody (Biolegend) at 1:100 for 30 minutes ... -
Evrogen
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2017, published in PLOS ONE doi: 10.1371/journal.pone.0184507Quote: Oligonucleotide primers for DOP-PCR and iDOP-PCR were synthesized by Evrogen JSC (Moscow ... -
Active Motif
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2020Quote: ... Mouse positive control primer set Actb2 (#71017, Active Motif) and mouse negative control primer set 1 (#71011 ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2022Quote: ... Sections were let to dry and covered with Fluorsave (Calbiochem, San Diego, CA, US). -
Merck
No products found because this supplier's products are not listed.Cited in OR1D2 receptor mediates bourgeonal-induced human CatSper activation in a G-protein dependent mannerbioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation ... -
TianGen Biotech
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ... -
Biosearch Technologies
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2021Quote: ... a set of oligonucleotides spanning the first intron of let-7-C was designed using the Stellaris Probe Designer software (Biosearch Technologies). The oligos were ordered from Biosearch Technologies labeled with Quasar-670. -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2022Quote: ... Genotypes were confirmed using primer sets recommended by Jackson Laboratory and Sinha lab. -
IDT DNA
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... these were run as two separate multiplex PCR “pools” (A & B) using the ARTIC version 3 primer set (ARTIC nCoV-2019 V3 Panel, IDT DNA Inc ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... RT-PCR products were purified using PCR Clean-up (Macherey-Nagel, 740609.250) following the manufacturer’s instructions ... -
Sanquin
No products found because this supplier's products are not listed.Cited in Staphylococcal superantigen-like protein 13 activates neutrophils via Formyl Peptide Receptor 2bioRxiv - Microbiology 2018, published in Cellular Microbiology doi: 10.1111/cmi.12941Quote: ... containing 0.05% HSA (Sanquin). HL-60 cells were purchased from ATCC ... -
GenScript
No products found because this supplier's products are not listed.Cited in Optogenetic actuator/ERK biosensor circuits identify MAPK network nodes that shape ERK dynamicsbioRxiv - Systems Biology 2022Quote: ... The following primers were used for the RT-qPCR reaction (designed with the Real-time PCR (TaqMan) Primer and Probes Design Tool from GenScript). -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 20nM siRNA against hsa-CHST15_0003 or hsa-TNFRSF21_0001 or a non-targeting siRNA control (GE Healthcare Dharmacon, Inc., Lafayette, CO, USA) using Lipofectamine 3000 (ThermoFisher Scientific) ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019Quote: ... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes. -
Origene Technologies
No products found because this supplier's products are not listed.Cited in A mean-field approach for modeling the propagation of perturbations in biochemical reaction networksbioRxiv - Pharmacology and Toxicology 2021Quote: ... Chop mRNA levels in treated cells were measured using quantitative RT-PCR as previously described [28] using validated primers (OriGene, Cat # HP207450). -
Primerdesign
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Nature Communications doi: 10.1038/s41467-019-12295-2Quote: ... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2020Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Preparations were let dry and observed at 400x magnification on a DMi8 fluorescence inverted microscope (LEICA®). Pictures were taken using a DFC3000 G camera coupled to the microscope. -
Chroma Technology
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2017, published in ACS Nano doi: 10.1021/acsnano.7b04743Quote: ... Fluorescence filter sets from Chroma Technology and Semrock were used ... -
Biotium Inc.
No products found because this supplier's products are not listed.bioRxiv - Pathology 2021Quote: ... Positive bands of appropriate size were confirmed using individual primer sets and resultant PCR products were visualized on a 1.5% agarose gel with gel red (Biotium). -
abcam
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: ... Cy5-labeled aptamer-chimeras were pre-complexed with HSA (Abcam, ab8030) at a 1:1 molar ratio for 30 minutes at room temperature and then added to the HUVECs in serum-free Opti-MEM media ... -
Eton Bioscience
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018, published in eLife doi: 10.7554/eLife.42995Quote: ... The PCR products were sequenced using Sanger sequencing with the same primers used for the PCR (Eton Bioscience). -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in Scientific Reports doi: 10.1038/s41598-020-60269-yQuote: ... the cells were incubated with DAPI diluted in PBS for 10 min at RT and washed before being mounted onto slides with Vectashield Hard Set (Vector Laboratories). Single-planed images were taken on a Nikon C2 confocal microscope using a 40X oil-immersion lens ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... Filter sets from Olympus and Chroma Technology Corp (49309 ET – Orange#2 FISH and 49310 ET – Red#2 FISH ... -
Nikon
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2022Quote: ... and corresponding filter sets (Nikon). Imaging conditions were optimized for each sample ... -
Carl Zeiss
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... Filter set 10 (Carl Zeiss) was used for separating the fluorescence and the excitation light ... -
GENEWIZ
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... were synthesised either by PCR using two overlapping primers or by GENEWIZ. They were inserted into the linear backbone by HiFi Assembly (NEB) ... -
R&D Systems
No products found because this supplier's products are not listed.Cited in Pharmacological CDK4/6 inhibition unravels a p53-induced secretory phenotype in senescent cellsbioRxiv - Cell Biology 2020Quote: ... human CXCL1/GROα duo-set or CCL5 duo-set or IGFBP3 duo-set (R&D Systems) were used to detect the concentrations of CXCL1 or CCL5 or IGFBP3 in the plasma following manufacturer’s instructions. -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... The RT-PCR were performed using AmpliTaq Gold DNA polymerase (Perkin Elmer, Boston, USA). PCR fragments were separated with electrophoresis using 1% agarose gel in tris-borate-EDTA (TBE ... -
TTP Labtech
No products found because this supplier's products are not listed.Cited in How the dual PDZ domain from Postsynaptic density protein 95 clusters ion channels and receptorsbioRxiv - Biophysics 2019Quote: ... Crystallisation trials were set up using a Mosquito (TTP Labtech) liquid handling robot ...