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No products found
because this supplier's products are not listed.
Kejia Zhang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... FLAG-tagged proteins were purified by incubating whole cell lysates from the transfected cell lines with 20 μL of DYKDDDDK-Tag Monoclonal Antibody Magnetic Microbead (Syd Labs) for three hours at 4 °C ...
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No products found
because this supplier's products are not listed.
Julieta Ramirez, et al.,
bioRxiv - Genomics 2023
Quote:
Whole cell lysates were separated on a 5% SDS/PAGE containing 20 μm Phos-tag (NARD Institute), followed by western blotting with anti-CTCF antibody.
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No products found
because this supplier's products are not listed.
Luis E. Martinetti, et al.,
bioRxiv - Neuroscience 2021
Quote:
... we used a virus-retrobead or saline-retrobead mixture (red RetroBeads, Lumafluor, Cat# R180). When comparing different AAV serotypes ...
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No products found
because this supplier's products are not listed.
Sheng Xiao, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected using a manual volume displacement injector (Narishige International USA, MMO-220A) connected to a glass pipette (Drummond Scientific ...
-
No products found
because this supplier's products are not listed.
Taeyong Kwon, et al.,
bioRxiv - Microbiology 2022
Quote:
... The same amount of the virus was mixed with 45 µL of human saliva (Lee Biosolutions) or medium and transferred into a 2 mL tube or onto stainless steel in the 12-well plate ...
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No products found
because this supplier's products are not listed.
Daniela Niemeyer, et al.,
bioRxiv - Microbiology 2021
Quote:
... and B.1.351 was assessed by a surrogate virus neutralization test (cPass Assay, Medac, Wedel, Germany) as described previously (Momsen Reincke et al. ...
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No products found
because this supplier's products are not listed.
Marie A Brunet, et al.,
bioRxiv - Genetics 2020
Quote:
... Human tissue lysates for altFUS endogenous expression were purchased from Zyagen Laboratories (San Diego ...
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No products found
because this supplier's products are not listed.
Vigneshwaran Venkatesan, et al.,
bioRxiv - Biochemistry 2022
Quote:
... the protein lysate was analysed for haemoglobin tetramer using G8 HPLC Analyzer (Tosoh). The globin chain analysis was performed using HPLC equipment with UV detector (Shimadzu ...
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No products found
Yan Qi, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... the presence of anti- Ad and anti-hemagglutinin antibodies was measured by ELISA against a purified Ad6 virus preparation (Greffex, Inc.) and a recombinant hemagglutinin protein (Creative Biomart). In the first case ...
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No products found
because this supplier's products are not listed.
Angelica F. Castañeda, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The clarified lysate was filtered through a 0.45 µm PES filter (Foxx Life Sciences). The protein was purified on an equilibrated HisTrap (GE Healthcare ...
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No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
... the clarified lysate was concentrated by tangential flow filtration at 5 L/min/m2 using a KR2i KrosFlo (Repligen) with Pellicon 2 mini cassettes (300 kDa ...
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No products found
because this supplier's products are not listed.
F. Esra Demircioglu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The lysate was immediately mixed with 0.1 M phenylmethanesulfonyl fluoride (PMSF) (50 μl per 10 ml lysate) and 250 units of TurboNuclease (Eton Bioscience), and cleared by centrifugation ...
-
No products found
because this supplier's products are not listed.
Jessica Corry, et al.,
bioRxiv - Biochemistry 2022
Quote:
... cells were single cell sorted using the CellenONE (Scienion) into 96 well plates in 20% conditioned media ...
-
No products found
because this supplier's products are not listed.
Madeline E. McCarthy, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Normal Peripheral Blood Mononuclear Cells (PBMCs) (Precision for Medicine; 10M cells/vial) are thawed and counted with a hemacytometer ...
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This product is a Zika Virus E and prM/M protein Reporter Virus Particles. Reporter Virus...
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Ami Patel, et al.,
bioRxiv - Immunology 2020
Quote:
... CHO cells stably expressing ACE2 were used as target cells (Creative Biolabs, Catalog No. VCeL-Wyb019). SARS-Cov-2 pseudovirus were titered to yield greater than 20 times the cells only control relative luminescence units (RLU ...
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No products found
because this supplier's products are not listed.
Min Yao, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Cells were seeded in the 24-well glass-bottom cell culture plate (Chemglass, #CLS-1812-024), and used 2-3 days later at 50-80% confluency ...
-
No products found
because this supplier's products are not listed.
Daniel A Skelly, et al.,
bioRxiv - Genomics 2019
Quote:
Cell lines were genotyped by Neogen Corp ...
-
No products found
because this supplier's products are not listed.
Runwei Yang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The filtered cells were subjected to 1× Red Blood Cell Removal Solution (Biogems, 64010-00-100, USA) for 5 min to remove red blood cells ...
-
No products found
because this supplier's products are not listed.
Sanjeev S. Ranade, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The cell pellet was resuspended in PBSF and a small aliquot was used for manual cell counting (SKC hemocytometer ...
-
No products found
because this supplier's products are not listed.
Michael Jewer, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... cells were cultured in a bioreactor (Synthecon) as previously described56.
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No products found
because this supplier's products are not listed.
D.M.K. Aladin, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The dissociated cells were gently pipetted 5 times to dissociate cell clumps before being deposited on a PLL-PEG-coated (SuSoS), glass-bottomed dish (IWAKI) ...
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No products found
because this supplier's products are not listed.
Sumnima Singh, et al.,
bioRxiv - Immunology 2020
Quote:
... Cell numbers were calculated using PerfectCount Microspheres (Cytognos). Data from at least 10,000 single viable CD45+ cells were acquired and analyzed by FlowJo software (v10.0.7).
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No products found
because this supplier's products are not listed.
Takefumi Kimura, et al.,
bioRxiv - Molecular Biology 2023
Quote:
LX-2 cells were obtained from Sigma-Aldrich and HepG2 cells were procured from Cellular Engineering Technologies ...
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No products found
because this supplier's products are not listed.
Byung-Chul Lee, et al.,
bioRxiv - Cell Biology 2023
Quote:
... CD33 chemically modified guide RNAs (6.25 μg/106 cells, Synthego, Redwood City, CA) and Cas9 protein (12.5 μg/106 cells, PNA Bio, Thousand Oaks, CA) were mixed for 10 minutes at room temperature ...
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No products found
because this supplier's products are not listed.
Shumeng Jiang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... passed through a 250 µm metal cell strainer (MIDSCI) using 1x HBSS solution (Gibco 24020-117) ...
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No products found
because this supplier's products are not listed.
Alexandra-Madelaine Tichy, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... cells were incubated with 10 μM PTX (List Labs) overnight (o/n ...
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No products found
because this supplier's products are not listed.
Jing Zeng, et al.,
bioRxiv - Genetics 2023
Quote:
Hemolysates were prepared from erythroid cells after 18 days of erythroid differentiation or engrafting erythroid cells using Hemolysate reagent (Helena Laboratories cat# 5125) and analyzed with D-10 Hemoglobin Analyzer (Bio-Rad).
-
No products found
because this supplier's products are not listed.
Amy M. LeMessurier, et al.,
bioRxiv - Neuroscience 2023
Quote:
... whole-cell and cell-attached recordings were obtained using a Fiberoptic Light Stimulating Device with 465nm blue LED (A-M Systems, Item# 726500) connected to a Fiber Optic Light guide (A-M Systems ...
-
No products found
because this supplier's products are not listed.
Kevin O’Dwyer, et al.,
bioRxiv - Pathology 2021
Quote:
... The cell pellet was resuspended in 20mL of PreservCyt (Hologic) and incubated for 20 minutes at room temperature ...
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No products found
because this supplier's products are not listed.
Hannah Weber, Rachel Ruoff, Michael Garabedian,
bioRxiv - Cancer Biology 2019
Quote:
... cells were double cross-linked with DSG (ProteoChem; cat # c1104) for 20 min and 1% formaldehyde for 10 min ...
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No products found
because this supplier's products are not listed.
Catherine R Knoverek, et al.,
bioRxiv - Biophysics 2021
Quote:
... we transformed our desired plasmid into BL21(DE3) cells (Intact Genomics) and grew cultures to an OD600 of 0.6 before we induced protein expression by adding 1 mM IPTG ...
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No products found
because this supplier's products are not listed.
Nana Naetar, et al.,
bioRxiv - Cell Biology 2020
Quote:
... cells were stained with an anti-mEos2 antibody (Badrilla, Leeds, UK) following the standard IF protocol.
-
No products found
because this supplier's products are not listed.
Stefano Musardo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... cells were incubated with primary antibody (Oxytocin, 1/10000 dilution, Immunostar #20068 ...
-
No products found
because this supplier's products are not listed.
Venny Santosa, Masato T. Kanemaki,
bioRxiv - Molecular Biology 2019
Quote:
... asynchronously growing cells were treated with 20 μM lovastatin (LKT Laboratories) for 24 h ...
-
No products found
because this supplier's products are not listed.
Mitchell R. Lewis, Tara L. Deans,
bioRxiv - Synthetic Biology 2023
Quote:
Mouse embryonic stem cells (harvested from a TARGATT mouse, Applied StemCell) (see Note 1).
-
No products found
because this supplier's products are not listed.
Yinghua Zhao, et al.,
bioRxiv - Microbiology 2024
Quote:
... the cells were stained with anti-dsRNA antibody (SCICONS, Cat# 10010200) and an Alexa Fluor 488-conjugated anti-mouse secondary antibody (Proteintech ...
-
No products found
because this supplier's products are not listed.
Noah R. Johnson, et al.,
bioRxiv - Neuroscience 2021
Quote:
... TgF344 rat cells were labeled with chicken anti-tau (PhosphoSolutions, 1:1,000), rabbit anti-Aβ (OC ...
-
No products found
because this supplier's products are not listed.
S. John Liu, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... labeled with primary antibodies including SOX10 to mark schwannoma cells (API 3099, Biocare; labeling validated in schwannoma and melanoma) ...
-
No products found
because this supplier's products are not listed.
Bidur Paudel, et al.,
bioRxiv - Neuroscience 2023
Quote:
... cells were generated using the Alt-R CRISPR-Cas9 System (IDT DNA Technologies) following the manufacturer’s protocol with the following modifications ...
-
No products found
because this supplier's products are not listed.
Rhalena A. Thomas, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Cells were incubated with primary antibodies: MAP2 (1:1000, EnCor Biotech CPCA-MAP2); Nestin (1:500 ...
-
No products found
because this supplier's products are not listed.
Jun Yu Ip, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... with absorbance at 254 nm monitored using a Triax Flow Cell (Biocomp Instruments). Monosome fractions were pooled and treated with 200 μg/mL Proteinase K (Roche ...
-
No products found
because this supplier's products are not listed.
Jason A Iskarpatyoti, et al.,
bioRxiv - Cell Biology 2021
Quote:
... supernatants were collected and cell pellet was lysed in 0.1% Triton-100X (Spectrum Chemical) in Tyrode’s buffer ...
-
No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
Peripheral blood mononuclear cells were obtained with a Ficoll-Hypaque gradient (Alere Technologies AS) from blood donors and monocyte populations (>90% CD14+ ...
-
No products found
because this supplier's products are not listed.
Benjamin Ng, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... the cell culture supernatant was concentrated using a Polyethylene glycol concentrating solution (90626, Chondrex) and collagen content was quantified using a Sirius red collagen detection kit (9062 ...
-
No products found
because this supplier's products are not listed.
Lewis D. Evans, et al.,
bioRxiv - Neuroscience 2020
Quote:
Genomic DNA was extracted from cell pellets using QuickExtract DNA Extraction Solution (Cambio; QE09050) as per the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Anupama Shirali, et al.,
bioRxiv - Immunology 2023
Quote:
... IgG control for the cell-penetration assay was obtained from Leinco technologies (I-118).
-
No products found
because this supplier's products are not listed.
Md Imtiaz Khalil, Arrigo De Benedetti,
bioRxiv - Cancer Biology 2022
Quote:
LNCaP and PC3 cells were treated with 1-5 μM of GLPG 0259 (Medkoo Biosciences, Inc. ...
-
No products found
because this supplier's products are not listed.
Henry De Belly, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were seeded in a 96-well #1.5 glass-bottom plates (Brooks Life Sciences; Chelmsford, MA) in R+B imaging media ...
-
No products found
because this supplier's products are not listed.
Aditi Madan, et al.,
bioRxiv - Physiology 2023
Quote:
... cells were incubated in Schneider’s Insect Medium without Amino Acids (United States Biological, Cat# S0100-01.10) for the indicated amount of time ...
-
No products found
because this supplier's products are not listed.
Lauren A. Callender, et al.,
bioRxiv - Immunology 2019
Quote:
... The cells were viewed in a Jeol 1010 transmission electron microscope (Jeol) and imaged using a Gatan Orius CCD camera (Gatan). Mitochondrial volume density (percentage of T cell volume occupied by mitochondria ...