-
No products found
because this supplier's products are not listed.
Jose L. Nieto-Torres, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The in vitro kinase reaction mixtures were incubated with 350 μl clarified lysate of HeLa LC3B-KO cells (8×105 cell equivalents, prepared as described above) and glutathione-sepharose beads (Bioworld) for 3 h at 4°C on a rotator ...
-
No products found
because this supplier's products are not listed.
Meredith J. Sigman, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Clarified lysates were combined with 2 μL of AGO4 antibody (Agrisera) or 2uL of rabbit IgG as a mock IP (Cell Signaling Technology) ...
-
No products found
because this supplier's products are not listed.
Milène Vandal, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The lysate was incubated with 100 U/mL salt-active nuclease (Arcticzymes) at 37°C for 1 h and then centrifuged at 2000 xg for 15 min ...
-
No products found
because this supplier's products are not listed.
Aleksi Isomursu, et al.,
bioRxiv - Biophysics 2020
Quote:
Cells that were collected for protein lysates were cultured on commercial hydrogel-coated 6-well plates (Matrigen, SW6-EC-0.5/SW6-EC-8/SW6-EC-50). These gels were similarly coated with 10 μg/ml of fibronectin before use.
-
No products found
because this supplier's products are not listed.
Sloan A. Lewis, et al.,
bioRxiv - Immunology 2021
Quote:
Circulating endotoxin was measured from plasma using a Limulus amebocyte lysate (LAL) assay (Hycult Biotech) following the manufacturers protocol.
-
No products found
because this supplier's products are not listed.
Eva Mittler, et al.,
bioRxiv - Microbiology 2020
Quote:
... cleared U2OS-Cas9 lysates were pre-incubated with the protease inhibitor E-64 (20 μM; Peptides International) for 20 min at room temperature (when indicated) ...
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No products found
because this supplier's products are not listed.
Minhui Guan, et al.,
bioRxiv - Microbiology 2024
Quote:
... Each test virus was diluted to a final concentration of 100 pM with 1 × kinetic buffer containing 10 µM oseltamivir carboxylate (American Radiolabeled Chemicals, St. Louis, MO) and zanamivir (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Rosalie Lipsh-Sokolik, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Xylanase activity was assayed using 100μl lysate and 0.5mM O-PNPX2 substrate (4-nitrophenyl beta-D-xylobioside, Megazyme) or cellPNP substrate (4-nitrophenyl beta-cellobioside ...
-
No products found
because this supplier's products are not listed.
Salwa Sebti, Zhongju Zou, Michael U. Shiloh,
bioRxiv - Physiology 2022
Quote:
... Cleared lysates were diluted in 2× SDS-PAGE loading buffer and submitted to western blotting using anti-p62 (GP62-C, Progen, 1:1,000 dilution), anti-LC3B (L7543 ...
-
No products found
because this supplier's products are not listed.
Lining Zheng, Sarith R. Bandara, Cecilia Leal,
bioRxiv - Bioengineering 2022
Quote:
... 4T1 cells (ATCC) and HeLa-Luc cells (Signosis) were cultured in full cell media consisting of Dulbecco’s modified Eagle’s medium (Corning) ...
-
No products found
because this supplier's products are not listed.
William Tyler Frantz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells from BMP-KO samples as well as non-melanocyte lineage keratinocytes were then removed ...
-
No products found
because this supplier's products are not listed.
Jeanette M. Metzger, et al.,
bioRxiv - Neuroscience 2022
Quote:
GFP-expressing human embryonic kidney cells (i.e., GFP-HEK cells, GenTarget Inc.) were used as an RNP delivery cell model ...
-
No products found
because this supplier's products are not listed.
Sanam Shafaatalab, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The cells were counted using the CellDrop automated cell counter (Froggabio, ON). The lysis buffer used contained 100 mM HEPES (pH= 8-8.5 ...
-
No products found
because this supplier's products are not listed.
Ni Zeng, et al.,
bioRxiv - Immunology 2021
Quote:
... Splenocytes depleted of T cells were used as antigen-presenting cells (APCs)/feeder cells and were irradiated in RS2000 (Rad Source Technologies) with a total dose of 30 Gy within a period of 10 mins ...
-
No products found
because this supplier's products are not listed.
Ragna S. Häussler, et al.,
bioRxiv - Biochemistry 2019
Quote:
... All secreted proteins were produced by using the transient Icosagen Cell Factory system with CHOEBNALT-85 cells and the QMCF Technology (Icosagen Cell Factory OÜ, Tartu, Estonia). Cells were maintained in a 50:50 mixture of 293 SFM II (Gibco ...
-
No products found
because this supplier's products are not listed.
Zixuan Liu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cell counting kit-8 (M4839, AbMole).
-
No products found
because this supplier's products are not listed.
K.G. Daniels, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... T cells were cryopreserved in RPMI1640 (UCSF cell culture core) with 20% human AB serum (Valley Biomedical, #HP1022HI) and 10% DMSO ...
-
No products found
because this supplier's products are not listed.
Amanda Acosta-Ruiz, et al.,
bioRxiv - Bioengineering 2019
Quote:
HEK 293T cells (authenticated by Bio-Synthesis, Inc ...
-
No products found
because this supplier's products are not listed.
M.A. Andres, et al.,
bioRxiv - Neuroscience 2022
Quote:
Human neural progenitor cells (hNPCs) (Neuromics, MN) were plated on Matrigel (Corning ...
-
No products found
because this supplier's products are not listed.
Oihana Iriondo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... cells were moved to hypoxic incubators (Nuaire NU-5831 for 4% O2 ...
-
No products found
because this supplier's products are not listed.
Sumeet Nayak, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... spotted (2.5μl of 105 cells/ml PBS cell suspension) and lysed on positively charged microscope slides (Globe Scientific, #1358W) by 7.5μl spreading buffer (0.5%SDS ...
-
No products found
because this supplier's products are not listed.
Shisu Zhao, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Cell counting kit 8 (CCK8) experiment for cell viability assay were performed using the CCK8 Kit (TargetMol, MA, USA). After a treatment ...
-
No products found
because this supplier's products are not listed.
Ryan S. Dhindsa, et al.,
bioRxiv - Genomics 2020
Quote:
... strained through 40 um cell strainers (MTC Bio) to remove cell clumps and collected for single-cell RNA-sequencing (scRNA-seq ...
-
No products found
because this supplier's products are not listed.
Juline Poirson, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Cells were treated with 1 µM MLN4924 (Chemietek) for 24 hours ...
-
No products found
because this supplier's products are not listed.
Daniel S Saxton, Jasper Rine,
bioRxiv - Molecular Biology 2019
Quote:
... RFP-expressing cells were diluted and plated at a density of ∼10 cells/plate (CSM-Trp (Sunrise Science Products, San Diego, CA), 1% agar) ...
-
No products found
because this supplier's products are not listed.
Midori Ohta, et al.,
bioRxiv - Cell Biology 2020
Quote:
... coding sequences optimized for human cell expression were synthesized (GENEWIZ). Sequences encoding Myc-tagged GIP-2 ...
-
No products found
because this supplier's products are not listed.
Ramireddy Bommireddy, et al.,
bioRxiv - Immunology 2022
Quote:
... and then 0.5% chicken red blood cells (Lampire Biological Laboratories) were added to determine HAI titers ...
-
No products found
because this supplier's products are not listed.
Allison J. Greaney, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were stained with recombinant biotinylated ACE2 (ACROBiosystems, AC2-H82E6) and serial dilutions of rCR3022 antibody for 1 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Makaía M. Papasergi-Scott, et al.,
bioRxiv - Neuroscience 2020
Quote:
... cells were treated with 5 μM CGP55845 (Hello Bio, Inc.). At 48 hours post-infection cells were harvested by centrifugation ...
-
No products found
because this supplier's products are not listed.
Frank W. Soveg, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were seeded on #1.5 12-mm coverslips (Bioscience Tools) coated with 10 μg/mL poly-L-ornithine hydrobromide (Sigma ...
-
No products found
because this supplier's products are not listed.
Stephen K. Dolan, et al.,
bioRxiv - Microbiology 2023
Quote:
... the cells were grown in Turbo Broth medium (Molecular Dimensions) (1 L ...
-
No products found
because this supplier's products are not listed.
Oded Danziger, et al.,
bioRxiv - Microbiology 2021
Quote:
... All cell lines were routinely tested (Boca Scientific #50-168-5641) negative for Mycoplasma contamination ...
-
No products found
because this supplier's products are not listed.
Tamara A. Potapova, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Transfected cells were selected with 1 mg/ml G418 (A.G. Scientific).
-
No products found
because this supplier's products are not listed.
Keng Hou Mak, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and were maintained in mesothelial cell growth medium (MSO-1, Zen-Bio, Inc.). Human cervical adenocarcinoma cell line HeLa from American Type Culture Collection was maintained in DMEM medium supplemented with 10% fetal bovine serum ...
-
No products found
because this supplier's products are not listed.
Tugsan Tezil, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Histones were extracted from HEK293T cells by EpiQuik Total Histone Extraction Kit (Epigentek), following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Elisa Matas-Rico, et al.,
bioRxiv - Cancer Biology 2021
Quote:
T cell migration was measured using 48-well chemotaxis chambers (Neuro Probe, Inc.) equipped with 5 µm-pore polycarbonate membranes (8 µm-pore for melanoma cells) ...
-
No products found
because this supplier's products are not listed.
Harry C. Tjondro, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Degranulated MPO (Dg-MPO) and cell death were monitored longitudinally in supernatants using ELISA (ICL LAB) and lactate dehydrogenase (LDH ...
-
No products found
because this supplier's products are not listed.
JN Rashida Gnanaprakasam, et al.,
bioRxiv - Immunology 2022
Quote:
... and glutamate in blank medium (without cells) and spent medium were measured by the bioanalyzer (YSI 2900). The metabolite consumption and production were determined by calculating the difference between blank and spent medium ...
-
No products found
because this supplier's products are not listed.
Trevor J. Hancock, et al.,
bioRxiv - Immunology 2022
Quote:
... as well as TGEV (transmissible gastroenteritis virus) (VMRD, Pullman, WA, USA). Normal cat serum was purchased from Jackson ImmunoResearch (West Grove ...
-
No products found
because this supplier's products are not listed.
Jianying Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus mixtures were subsequently mixed at a ratio of 1:1 with PBS-washed sheep blood cells (Hemostat laboratories, Galveston, TX, USA). Aliquots of blood meals were collected to verify the initial virus proportions in the inoculum ...
-
No products found
because this supplier's products are not listed.
Rueyhung R. Weng, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5% human platelet lysate (PLS1, Compass Biomedical), 70 ng/mL IL-15 (AF-200-15 ...
-
Cat# 158765-79-8,
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Minjoo Kim, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The resulting whole cell lysate was then tumbled with N,N-dimethyl-N-dodecylglycine (Empigen BB Detergent, BOC Sciences) (3% v/v ...
-
No products found
Oliver Hsia, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Lysate was filtered through a BioPrepNylon Matrix Filter (BioDesign) then incubated with 1 mL Ni-NTA resin per litre culture for 1 hour ...
-
No products found
because this supplier's products are not listed.
Dora Buzas, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Control wells containing virus only (no ADAH11) as well as a positive control (a commercial monoclonal antibody (Absolute Antibody; Sb#15) recognising the S protein RBD ...
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Cell viability Assay Kit (CQBL-10K)
Cat# CQBL-10K,
1.0 kit, 10000 tests, USD $279.0
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Liliana M. Sanmarco, et al.,
bioRxiv - Immunology 2023
Quote:
Pyruvate levels were quantified in BMDC lysates after 1h stimulation using EnzyChrom pyruvate assay kit (EPYR-100, BioAssay Systems), followed the procedure suggested by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Akiko Ogawa, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... cells were incubated with urantide (Peptide Institute) for 30 min at 100 nM (for UTS2R inhibition) ...
-
No products found
because this supplier's products are not listed.
Jayanth S. Shankara Narayanan, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... anti-Rat HRP Polymer (Cell IDX, 2AH-100) or anti-Rabbit HRP Polymer (Cell IDX ...
-
No products found
because this supplier's products are not listed.
Richard Gerum, et al.,
bioRxiv - Biophysics 2022
Quote:
... 1 ml of alginate solution are then added to the cell pellet of 106 cells in the Falcon tube and mixed using a positive displacement pipette (15314274, Gilson/Fisher Scientific) by slowly (∼ 2 s cycle time ...
-
No products found
because this supplier's products are not listed.
Christophe Chapard, et al.,
bioRxiv - Genetics 2023
Quote:
... Yeast cells were synchronized in G1 by adding α-factor (Proteogenix, WY-13) in the media every 30 min during 2h30 (1 μg/mL final) ...
-
No products found
because this supplier's products are not listed.
Louis J. Born, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The cell monolayer was then scratched using a 200 μL pipette tip (Rainin, 30389243). Media was then replaced using the same serum starving conditions ...