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No products found
because this supplier's products are not listed.
Yannick O Alexandre, et al.,
bioRxiv - Immunology 2020
Quote:
... were coated overnight at 4°C with 10 μg/ml HSV-1 inactivated Vero cell extract (Advanced Biotechnologies). Unbound protein was washed away (PBS 0.05% Tween20 ...
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No products found
because this supplier's products are not listed.
Flavia Chiuppesi, et al.,
bioRxiv - Immunology 2020
Quote:
... or vesicular stomatitis virus G (VSV-G, Aldevron), using FuGENE6 (Roche ...
-
No products found
because this supplier's products are not listed.
Sara Al Rawi, et al.,
bioRxiv - Neuroscience 2021
Quote:
Fbxo7 was immunoprecipitated from cell lysates using 2μl of anti-Fbxo7 antibody (Aviva), or isotype matched control ...
-
No products found
because this supplier's products are not listed.
Cecilia Östlund, et al.,
bioRxiv - Cell Biology 2022
Quote:
... the cell lysates were homogenized with a Wheaton Dounce homogenizer (DWK Life Sciences), underlaid with a 40% sucrose cushion ...
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Milica Moskovljevic, et al.,
bioRxiv - Immunology 2024
Quote:
... Cells stimulated with either lysates of CMV-infected fibroblasts (Virusys, 10 μg/mL), overlapping Gag 15mer peptides (HIV-1 Gag peptide pool ...
-
No products found
because this supplier's products are not listed.
Hilda Mirbaha, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Lysate was homogenized by GEA Niro Soavi’s PandaPLUS 2000 ...
-
No products found
because this supplier's products are not listed.
Angela Ma, et al.,
bioRxiv - Microbiology 2022
Quote:
... adenovirus (D3Ultra Respiratory Virus Screening & Identification kit, Quidel, San Diego, CA), and cytomegalovirus (D3 DFA Cytomegalovirus Immediate Early Antigen Identification kit ...
-
No products found
because this supplier's products are not listed.
Katharina Kases, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and anti- ZC3H11A (Atlas Antibodies, heavy lysate) antibodies in Protein LoBind tubes (Eppendorf) ...
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No products found
because this supplier's products are not listed.
Danny Galleguillos, et al.,
bioRxiv - Neuroscience 2020
Quote:
... BDNF and IGF-1 in cell medium and cell lysates of mouse primary microglia were measured using Mature BDNF Rapid ELISA kit (Biosensis, BEK-2211-1P) and Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems ...
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No products found
because this supplier's products are not listed.
Youssouf Sereme, et al.,
bioRxiv - Microbiology 2020
Quote:
... A poliovirus serology-positive control serum (Poliomyelitis virus kit, GenWay, San Diego, California, USA) was also used as a control ...
-
No products found
because this supplier's products are not listed.
Surya Cayre, et al.,
bioRxiv - Developmental Biology 2020
Quote:
The following primary antibodies were used on mouse cell-derived lysates: anti-mouse milk proteins (Accurate Chemical; YNRMMSP; 1/2000), anti-mouse b-casein (a kind gift from C ...
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No products found
because this supplier's products are not listed.
Susanne A. Wycislo, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Equivalent amounts of “light”-labeled control and “heavy”-labeled GFP–containing lysates or “light”-labeled control and “heavy”-labeled GFP–containing lysates were incubated (separately) with GFP-Trap agarose beads (Allele Biotechnology, San Diego, CA) for 30 min at 4°C ...
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No products found
because this supplier's products are not listed.
Orest V. Matveichuk, et al.,
bioRxiv - Immunology 2023
Quote:
... Protein concentration in the lysates was measured with BradfordUltra (Expedeon), the lysates were supplemented with sample buffer and subjected to 10% SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Nicole L. Welch, et al.,
bioRxiv - Microbiology 2020
Quote:
... Marbled eel adomavirus virions were purified from lysates of infected EK-1 cells using Optiprep gradient ultracentrifugation (Peretti, FitzGerald et al. 2015). DNA extracted from Optiprep gradient fractions was subjected to rolling circle amplification (RCA ...
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No products found
because this supplier's products are not listed.
Han Kang Tee, et al.,
bioRxiv - Microbiology 2019
Quote:
... The viral loads in organs were determined using TaqMan fast virus 1-step master mix (ABI, USA). One step RT-PCR was also performed to amplify viral RNA from the organs using MyTaq One-Step RT-PCR kit (Bioline ...
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No products found
because this supplier's products are not listed.
Caire Robin, et al.,
bioRxiv - Cell Biology 2022
Quote:
... aureus load of cell lysates was quantified on an agar plate using an automatic plate seeder (EasySpiral Dilute, Interscience, St-Nom la Bretèche, France) and a colony counter (Scan 4000 ...
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No products found
because this supplier's products are not listed.
Bioy Alexis, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... supernatants (1 μg/mL of lysate) were treated with DNAse I (Eurogentec) for 1 hour on ice ...
-
No products found
because this supplier's products are not listed.
Avital Licht-Murava, et al.,
bioRxiv - Neuroscience 2022
Quote:
... per ml culture medium at DIV 8 using Lipofectamine 3000 5 h before infection with vesicular stomatitis virus (VSV) at 100 MOI or with adenovirus-eGFP (Ad5CMV-eGFP, lot #ad3586, Viral Vector Core Facility, Carver College of Medicine ...
-
No products found
because this supplier's products are not listed.
Ben Vanderkruk, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Insulin concentration was measured in conditioned medium and islet lysate by ELISA (Alpco). DNA concentration of islet lysate was determined by Qubit dsDNA HS Assay (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
David L. Haggerty, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Flash frozen brain lysates were homogenized using a BeadBug™ 6 (Benchmark scientific Cat No ...
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No products found
because this supplier's products are not listed.
Nikol Dibus, et al.,
bioRxiv - Cell Biology 2023
Quote:
... lysates were incubated with the PD-1 antibody (1 µg; Exbio #11-176) and mixed with Dynabeads® Protein G ...
-
No products found
because this supplier's products are not listed.
Brenton T. Laing, et al.,
bioRxiv - Neuroscience 2022
Quote:
Immunostaining was conducted for axonal projections and verification of virus expression using a chicken anti-GFP polyclonal antibody (1:1000, Cat # GFP-1020, RRID:AB_10000240; Aves Labs, CA, U.S.A.) or a rabbit anti-DsRed polyclonal antibody (1:1000 ...
-
No products found
because this supplier's products are not listed.
Michael J Massare, et al.,
bioRxiv - Immunology 2021
Quote:
... pBac1 plasmids were transfected into Sf9 with Flash-bacGOLD bacmid containing the Autographa californica polydedrosis virus genome (Oxford Expression Technology, Oxford UK). Sf9 cells cultures were infected with recombinant baculovirus expressing the HA genes ...
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No products found
because this supplier's products are not listed.
Rongkui Han, et al.,
bioRxiv - Genetics 2020
Quote:
... 600 μL of clear lysate was transferred to DNA binding plates (Epoch Life Sciences EconoSpin™ 96 well) stacked over a 1 mL collection plate and centrifuged 5 minutes (RT ...
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No products found
because this supplier's products are not listed.
Kuan-Yi Lu, et al.,
bioRxiv - Microbiology 2020
Quote:
... 5 mg parasite protein lysate was precleaned with 600 μL uncoated agarose beads (Echelon Biosciences, Salt Lake City, UT) at 4 °C for 90 min ...
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No products found
because this supplier's products are not listed.
Nitchakarn Kaokhum, et al.,
bioRxiv - Biochemistry 2022
Quote:
DUB activity in cartilage tissue lysates were measured using ubiquitin-rhodamine(110)-glycine quenched substrate (Boston Biochem, cat No U-555). In the presence of active DUBS ...
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No products found
because this supplier's products are not listed.
Alakesh Singh, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were stained to estimate EdU positive cells using Sulfo-Cyanine5 azide (Lumiprobe, USA) as per the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Maria Maloverjan, et al.,
bioRxiv - Cell Biology 2021
Quote:
... cells were incubated in cell culture medium containing 10 μM Draq5 DR50200 reagent (BioStatus, UK) for 3 min at 37 °C ...
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No products found
because this supplier's products are not listed.
Sebastian Müller, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Circulating cancer cells were grown in stem cell ECM T75-flasks (Celprogen, # E36102-29-T75) and ECM 6-well plates (Celprogen ...
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No products found
because this supplier's products are not listed.
Beerend H.J. Winkelman, et al.,
bioRxiv - Neuroscience 2019
Quote:
... amplified (X3+Cell; FHC, Bowdoinham, ME), digitized at 15 kHz (Power1401 ...
-
No products found
because this supplier's products are not listed.
Didem Ilter, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... HEK293T cells were obtained from GenHunter. All cell line were maintained at 37°C and 5% CO2 in the presence of 100 unit/ml penicillin and 100 μg/ml streptomycin (Gibco ...
-
No products found
because this supplier's products are not listed.
Georg Petkau, et al.,
bioRxiv - Immunology 2022
Quote:
... Cell numbers per generation were enumerated using cell counting beads for flow cytometry (ACBP-50-10, Spherotech).
-
No products found
because this supplier's products are not listed.
John C Kostyak, et al.,
bioRxiv - Immunology 2024
Quote:
... Blood cell analysis was carried out using a Hemavet blood cell analyzer (Drew Scientific, Miami Lakes, FL).
-
No products found
because this supplier's products are not listed.
Jen M. Hope, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
PC12 cells (NeuroScreen-1 subclone, Cellomics, discontinued) were maintained in complete medium (F12K supplemented with 15% horse serum and 2.5% FBS ...
-
No products found
because this supplier's products are not listed.
Mark A. Skylar-Scott, et al.,
bioRxiv - Bioengineering 2020
Quote:
HUVEC-RFP cells (Angio-Proteomie, #cAP-0001RFP), WT-eGFP PGP1 cells ...
-
No products found
because this supplier's products are not listed.
Yingying Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... rabbit red blood cell (Complement technology, B300) was washed in GVBo (Ca2+ and Mg2+ free ...
-
No products found
because this supplier's products are not listed.
Jonathan M. Goodwin, et al.,
bioRxiv - Cell Biology 2021
Quote:
HeLa cells or U2OS cells were made to stably express Cas9 through lentiviral transduction (vector Cat# SVC9-PS-Hygro, Cellecta). Knockout cell lines were generated as pooled populations following subsequent lentiviral transduction with gRNA sequences as indicated (vector Cat# SVCRU6UP-L ...
-
No products found
because this supplier's products are not listed.
Mercè Gomar-Alba, et al.,
bioRxiv - Cell Biology 2021
Quote:
... resuspended in 200 μl of ice-cold washing buffer and subsequently cell wall was digested by incubating 100 μl of cells with 0.4 mg/ml of Zymolyase 100T (SEIKAGAKU CORPORATION) at 30 °C for 5-15 min ...
-
No products found
because this supplier's products are not listed.
Daniel D. Lam, et al.,
bioRxiv - Genetics 2021
Quote:
... cells were stained with methyl green pyronin (Dianova). In the case of efficient lysis ...
-
No products found
because this supplier's products are not listed.
Murilo Delgobo, et al.,
bioRxiv - Immunology 2019
Quote:
... cells were stained with Hoechst 33342 (Immunochemistry technologies) for 2h ...
-
No products found
because this supplier's products are not listed.
Svetlana Glushakova, et al.,
bioRxiv - Biophysics 2022
Quote:
... The mechanical activation of PIEZO1 in RBC by shear stress was achieved by constant agitation of cells (1-2 × 108 cells/ml) on the platform of a “Belly Button Shaker” (Model BBUAAUV15, Ibi Scientific) at a speed of 14 rpm ...
-
No products found
because this supplier's products are not listed.
Shelby A. Roseman, et al.,
bioRxiv - Genomics 2023
Quote:
... cells were resuspended and fixed at a density of 106 cells/mL in media containing 10% FBS (Peak Serum, PS-FB2) and 1% formaldehyde (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Ana Kucera, et al.,
bioRxiv - Immunology 2020
Quote:
... T cell cultures were re-stimulated with DCs and 10 days later T cells were stained with MART-1 dextramer (Immudex, Copenhagen, Denmark) to assess the presence of MART-1 antigen-specific T cells in the cultures.
-
No products found
because this supplier's products are not listed.
P. Malaka De Silva, et al.,
bioRxiv - Microbiology 2021
Quote:
... The percentage of GFP expressing cells were determined using the cells not exposed to ciprofloxacin as a negative control using FCS Express version 7 (De Novo Software). Four individual biological replicates were tested for each plasmid.
-
No products found
because this supplier's products are not listed.
Lucia Leitner, et al.,
bioRxiv - Developmental Biology 2024
Quote:
Total RNA was extracted from single cell suspensions of 500,000 CSCs or CB-derived cells using the FavorPrep™ Blood/Cultured Cell Total RNA Mini Kit (Favorgen, FABRK001-2), eluted with 40μl RNase-free ddH2O and the RNA concentration was measured with a Nanodrop 2000c spectrophotometer (Thermo Scientific) ...
-
No products found
because this supplier's products are not listed.
Keith F. DeLuca, et al.,
bioRxiv - Cell Biology 2021
Quote:
... cells were treated with 10 μM reversine (Adooq Biosciences) for 1 hr prior to fixation for immunofluorescence or harvesting for immunoblot analysis ...
-
No products found
because this supplier's products are not listed.
Mathieu Paquette, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cells were incubated in 5% BSA (Bioshop Canada, ALB001.500) in PBS for 1 h and then with TFEB or TFE3 primary antibodies in 5% BSA in PBS for 2.5 h at 37°C ...
-
No products found
because this supplier's products are not listed.
Jian Zhang, et al.,
bioRxiv - Immunology 2022
Quote:
... were cocultured with autologous memory B cells (5×104 cells) in the presence of 100 ng/ml staphylococcal enterotoxin B (SEB) (Toxin Technology, Sarasota, FL, USA) and RPMI 1640 medium supplemented with 10% FBS in 96-well U-bottom plates for 6 days ...
-
No products found
because this supplier's products are not listed.
Nathaniel H. Thayer, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were then moved using a microdissection needle (Singer Instruments) to a unique position on the plate ...
-
No products found
because this supplier's products are not listed.
Martin P. Schwalm, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... was added to the cells using an Echo acoustic dispenser (Labcyte) and the cells were incubated additional 20h at 37 °C/5 % CO2 ...