-
No products found
because this supplier's products are not listed.
Jason Neidleman, et al.,
bioRxiv - Immunology 2020
Quote:
... or Influenza Virus Control Peptide Pool (Anaspec). As a positive control for cytokine detection ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Alex J. Guseman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... or N234A Spike in Vero E6 cells and quantifying HIV-1 capsid (p24) concentrations by ELISA (XpressBio).
-
No products found
because this supplier's products are not listed.
Katharina M. Scherer, et al.,
bioRxiv - Microbiology 2021
Quote:
... Vero cells were plated at 60% confluence in 50 mm glass bottom dishes (GWST-5040, Willco Wells BV) the day before infection ...
-
No products found
because this supplier's products are not listed.
Jyoti Batra, et al.,
bioRxiv - Microbiology 2020
Quote:
... Clarified cell lysates were incubated with anti-GFP (MBL International Corp.), -HA or -FLAG magnetic beads (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Paula Lagan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Successful virus isolation was confirmed either by haemagglutination of 0.5% chicken blood cells (Envigo #S.B-0008) or by RRT-PCR.
-
No products found
because this supplier's products are not listed.
Shawn Freed Jr, Nancy D. Hanson,
bioRxiv - Microbiology 2023
Quote:
... Whole cell lysates were collected by bead-beating (Next Advance Bullet Blender) and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay ...
-
No products found
because this supplier's products are not listed.
Michelle N. Vu, et al.,
bioRxiv - Microbiology 2021
Quote:
TMPRSS2-expressing Vero E6 or Calu-3 2B4 cells were pretreated with 50-100 μM of K11777 (AdipoGen #AG-CR1-0158-M005) or 50-100 μM of camostat mesylate (Sigma-Aldrich #SML0057-10MG) ...
-
No products found
because this supplier's products are not listed.
Laura Cheradame, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Cell lysate protein concentration was determined using a Micro BCA Protein Assay kit (Bio Basic). Unless specified ...
-
No products found
because this supplier's products are not listed.
Xiaohui Zhao, et al.,
bioRxiv - Biochemistry 2021
Quote:
... cell lysates prepared as above were labeled with azido-PEG3-biotin (Click Chemistry Tools, AZ104), incubated with high-capacity neutravidin beads (Thermo Scientific Pierce) ...
-
No products found
because this supplier's products are not listed.
Eleni Petsouki, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Nde1-null C3H10T1/2 or double mutant cell lysates were incubated with α-FBW7 antibody (Abnova) overnight to immunoprecipitate FBW7 ...
-
No products found
because this supplier's products are not listed.
Marcus Buggert, et al.,
bioRxiv - Immunology 2020
Quote:
... bulk memory or virus-specific CD8+ T cells were sorted into cold fetal bovine serum and frozen in RNAzol (Molecular Research Center). TCRβ transcripts were amplified using a template-switch anchored RT-PCR ...
-
No products found
because this supplier's products are not listed.
Matthew D. Lauver, et al.,
bioRxiv - Immunology 2022
Quote:
... Virus dilutions were combined 1:1 with 0.45% sheep erythrocytes (Innovative Research) and incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Jieqiong Qu, et al.,
bioRxiv - Microbiology 2023
Quote:
... CHIKV virus stock (5 × 108 TCID50/mL) and full human blood (Sanquin) was 1:2 mixed to make the infectious blood meal with a final virus titer of 1.7× 108 TCID50/ml ...
-
No products found
because this supplier's products are not listed.
Cinzia Klemm, Gudjon Olafsson, Peter H Thorpe,
bioRxiv - Cell Biology 2023
Quote:
3xFLAG-tagged Mif2CENP-C cells were harvested after 4 hours of growth at restrictive (37°C) or permissive (23°C) temperature and whole cell lysates were prepared using glass beads and a cell disrupter (Scientific Industries Inc.) in Laemmeli buffer containing an EDTA-free protease inhibitor cocktail (Thermo Fisher) ...
-
No products found
because this supplier's products are not listed.
Delphyne Descamps, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cell lysates were incubated for 4 h at 4°C with an anti-Flag antibody coupled to agarose beads (Euromedex). The beads were then washed 3 times with lysis buffer and 1 time with PBS ...
-
No products found
because this supplier's products are not listed.
Naureen Javeed, et al.,
bioRxiv - Cell Biology 2019
Quote:
... total protein lysates was isolated using NP-40 buffer (Amresco, J619) supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology ...
-
No products found
because this supplier's products are not listed.
M. Julhasur Rahman, et al.,
bioRxiv - Microbiology 2021
Quote:
The mCherry-E3L integration sites in the purified HGT virus genomes were located by inverse PCR amplification and by PacBio sequencing ...
-
No products found
because this supplier's products are not listed.
Melissa H. Bergeman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Image segmentation and 3D rendering of HSV-1 virus particle undergoing exocytosis from Rab6a vesicle was done in Imaris (Oxford Instruments) by constructing spots and surfaces for the objects at respective image slices ...
-
No products found
because this supplier's products are not listed.
Xiaofeng Zheng, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Insulin and protein content of the lysate were measured using Mouse Insulin ELISA (Mercodia) and BCA assay (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Nitin Raj, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... the pre-cleared lysates were incubated with 1-2 µg Mettl3 polyclonal antibody (Abclonal, A8370) or 1-2 µg Mettl14 polyclonal antibody (Abcam ...
-
No products found
because this supplier's products are not listed.
Michael J. Cohen, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cytosolic lysate was covalently modified with or without 1mg/mL sulfo-NHS-LC biotin (ApexBio) for 1 hour ...
-
No products found
because this supplier's products are not listed.
Julie M. Button, Suchetana Mukhopadhyay,
bioRxiv - Microbiology 2021
Quote:
Five microliters of purified virus or pelleted cores were placed on a Formvar and carboncoated 300 mesh grid (Ted Pella Inc., Redding, CA) and stained with 1% uranyl acetate ...
-
No products found
because this supplier's products are not listed.
Flavia Ferrantelli, et al.,
bioRxiv - Microbiology 2022
Quote:
... virus dilutions and number of deaths/group after challenge were used to calculate the LD50 (Quest Graph™ LD50 Calculator, AAT Bioquest, Inc.). The Kaplan-Meier curve was used to show survival rate differences among groups of animals challenged with different doses of SARS-CoV-2 virus ...
-
No products found
because this supplier's products are not listed.
Paola Munoz-Tello, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
-
No products found
because this supplier's products are not listed.
Kelly T. Rios, et al.,
bioRxiv - Microbiology 2024
Quote:
The gametocyte and zygote lysates were mechanically lysed with a 1 mL Dounce homogenizer (Wheaton, Cat# 357538) for one minute on ice with a tight pestle following chemical lysis ...
-
No products found
because this supplier's products are not listed.
Aleksandr Andriianov, et al.,
bioRxiv - Microbiology 2023
Quote:
... Wells were screened by PCR for the presence of lysate with recombinant phages and T3Δ0.3 was further purified from individual plaques and verified by Sanger sequencing (Evrogen).
-
No products found
because this supplier's products are not listed.
Heather Felgate, et al.,
bioRxiv - Genomics 2022
Quote:
... For Illumina sequencing DNA was extracted from the lysate with the Quick-DNA Fungal/Bacterial 96 kit (Cambridge Bioscience), in accordance with the manufacturer’s guidelines ...
-
No products found
because this supplier's products are not listed.
Qiyue Ding, et al.,
bioRxiv - Biochemistry 2020
Quote:
... resuspended in the Lysate Buffer (supplied in the -SulfoBiotics- Protein Redox State Monitoring Kit Plus, Dojindo Molecular Technologies Inc., Rockville, MD, USA) supplemented with phenylmethylsulfonyl fluoride (2 mM) ...
-
No products found
because this supplier's products are not listed.
Daniel Ellis, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL quartz cell (Starna Cells, Inc.) and absorbance was measured from 180 to 1000 nm ...
-
No products found
because this supplier's products are not listed.
Sourav Banerjee, Rupak Datta,
bioRxiv - Cell Biology 2019
Quote:
... J774A.1 cells (murine macrophage cell line obtained from National Center for Cell Sciences, Pune) were grown in Dulbecco’s modified Eagle’s medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Guodong Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Cells were harvested by cell lifter (Biologix, 70-2180) 72-h after co-transfection with PHP.eB (Addgene ...
-
No products found
because this supplier's products are not listed.
Hope M. Healey, Susan Bassham, William A. Cresko,
bioRxiv - Genetics 2021
Quote:
... we filtered cells through a 40uM cell strainer (Thomas scientific 1181X52) then washed the original tube twice with 100ul of 0.04% BSA in PBS and poured over the same cell strainer.
-
No products found
because this supplier's products are not listed.
Young-Jun Choi, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cells were filtered through a 40 micron Flowmi cell strainer (Bel-Art) before flow sorted using Aria B (Becton ...
-
No products found
because this supplier's products are not listed.
Kung-Chi Chang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Invasion assays with 4T1 MaTAR25 knockout cells and 4T1 control cells were carried out using the Cultrex® 24 well BME Cell Invasion Assay (Trevigen) following the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Aline Pfefferle, et al.,
bioRxiv - Immunology 2019
Quote:
... The labeled NK cells were resuspended in Stem Cell Growth Medium (SCGM; CellGenix) supplemented with 10% human serum (Sigma) ...
-
No products found
because this supplier's products are not listed.
Saria Mcheik, et al.,
bioRxiv - Immunology 2019
Quote:
... Absolute cell numbers were determined by incorporating beads in the cell suspension (15 µm, Bangs Laboratories) and acquiring 15,000 beads ...
-
No products found
because this supplier's products are not listed.
Euido Nishijima, et al.,
bioRxiv - Neuroscience 2022
Quote:
Transient transfection in Cos-7 cells or Neuro2A cells was performed using Polyethylenimine HCl Max (Polyscience). After transfection for 20 h ...
-
No products found
because this supplier's products are not listed.
Zhuangzhuang Geng, et al.,
bioRxiv - Biochemistry 2021
Quote:
... These cells and normal HEK293T cells were maintained in a standard DMEM medium containing 10% FBS (Atlanta Biologicals, Cat# S11050), L-glutamine ...
-
No products found
because this supplier's products are not listed.
Lisa-Marie Kuhl, et al.,
bioRxiv - Genetics 2020
Quote:
... Cell lysates were mixed on a VXR basic Vibrax (IKA) for 2 min at 1500 rpm ...
-
No products found
because this supplier's products are not listed.
Saliha Musovic, et al.,
bioRxiv - Physiology 2021
Quote:
... P2Y2R protein levels were determined in cell lysate with specific mouse ELISA (catalog # MBS7200782, MyBioSource).
-
No products found
because this supplier's products are not listed.
Ella Doron-Mandel, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Two mg of cell lysate at 20-50mg/ml were loaded onto a Yarra SEC-4000 column (Phenomenex 00H-4514-K0 ...
-
No products found
because this supplier's products are not listed.
Paulo J. da Costa, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Protein lysates mixed with 1x SDS-PAGE Sample Loading Buffer (NZYtech) were denatured at 95°C for 10 minutes and centrifuged at 500g for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Magen E. Francis, et al.,
bioRxiv - Microbiology 2021
Quote:
... to confirm stability of the SARS-CoV-2 virus after culture in vDMEM (DMEM (Dulbecco’s Modified Eagle Medium) (Wisent Bioproducts (Cat # 319-005-CL)) ...
-
No products found
because this supplier's products are not listed.
DaoFei Song, Lei Yin, Chang Wang, XiuYing Wen,
bioRxiv - Molecular Biology 2019
Quote:
... The protein concentration in tissue lysates was measured with a BCA protein assay kit (Boster, Wuhan, China) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Gordon Rix, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... Lysate was clarified by spinning 14,000g for 20 min at 4 °C (New Brunswick Avanti J-30I). Protein was purified over hand-packed HisPur™ Ni-NTA Resin (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Zachary JS Mays, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Ten microliters of lysate were then mixed with 190 μL of pre-warmed 50 mM phe (Tokyo Chemical Industry, Portland, OR) in phosphate buffered saline (PBS ...
-
No products found
because this supplier's products are not listed.
Ameya P. Jalihal, et al.,
bioRxiv - Cell Biology 2020
Quote:
... For live cell imaging cells were seeded on Delta T dishes (Bioptechs, 04200417C) and imaged on a Bioptechs temperature control module (Bioptechs ...
-
No products found
because this supplier's products are not listed.
Yurika Ito, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Single iPS cells carrying an inducible MYOD1 activation system were expanded in Primate ES cell medium (Reprocell) without bFGF and with 10µM of Y-27632 (Nacalai tesque ...
-
No products found
Fia B. Larsen, et al.,
bioRxiv - Biochemistry 2023
Quote:
Cells were treated with bortezomib (LC Laboratories) and chloroquine (Sigma ...