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No products found
because this supplier's products are not listed.
William B. Klimstra, et al.,
bioRxiv - Microbiology 2020
Quote:
Confluent monolayers of Vero-E6 cells in 35 mm-glass bottom dishes (Ibidi) were infected with P3 Munich virus at a multiplicity of infection (MOI ...
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No products found
because this supplier's products are not listed.
Li Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cell lysates and recombinant ACE2 protein control (Sino Biological) were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2 ...
-
No products found
because this supplier's products are not listed.
Xiaochen Wang, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... cell lysate was immunoprecipitated with anti-Flag antibody (Proteintech, 80010-1-RR) and Protein A/G magnetic beads (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Michelle A. Johnson, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Cell lysate was immunoprecipitated with Magstrep Type3 beads (IBA Lifesciences; 2-4090-002) overnight with end/end rocking at 4°C following the protocol provided by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Satoshi Matsui, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cell lysate was sonicated by Covaris S220 (Covaris ...
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No products found
because this supplier's products are not listed.
Christopher M. Hoel, et al.,
bioRxiv - Biophysics 2022
Quote:
... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Dawei Zhou, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Percentages of virus-infected cells was quantified by using the Gen5 Image+ software (BioTek).
-
No products found
because this supplier's products are not listed.
Kapil Gupta, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Stitched images of 9 fields covering the central 50% of the well were analyzed for virus infected cells using Cell Reporter Xpress software (Molecular Devices). The cell number was determined by automated counted of DAPI stained nuclei and infected cells were identified as those cells in which positive N staining was detected associated with nuclear DNA staining.
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No products found
because this supplier's products are not listed.
Nour Zeaiter, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or 10 μg human ACSS2-overexpressing HEK-293 cell lysate (ref. LY412981, Origene, MD ...
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No products found
because this supplier's products are not listed.
Elsie Yekwa, et al.,
bioRxiv - Biochemistry 2019
Quote:
... titration and infection of Vero E6 cells (VERO C1008 [Vero 76, clone E6, Vero E6] ATCC® CRL-1586™ (ATCC, LGC Standards, Molsheim, France) are described in [44] ...
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This kit is faster, stable, and more sensitive than the traditional soft-agar assay.
Cat# KITA1039,
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Yunjeong Kim, et al.,
bioRxiv - Microbiology 2021
Quote:
... Vero E6 cells expressing human TMPRSS2 (Vero-TMPRSS2) cells were obtained from Creative Biogene (Shirley, NY)[88] ...
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Cell strainers (Falcon), components of the NCIS kit. Suitable for removal of tissue debris in...
Cat# LK003265,
5 ea, $50.00
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Richard J Mills, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The inoculum for Vero cells incorporated TPCK-treated Trypsin (Worthington Biochemical Corporation) at 1 μg/ml ...
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No products found
because this supplier's products are not listed.
Ruiting Shen, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Vero cells were seeded in 12-well plates including 20 mm coverslips (Solarbio, China) and pre-treated with 50 μM ...
-
No products found
because this supplier's products are not listed.
Galina Popova, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... This RNA was then introduced to Vero cells using TransIT-mRNA transfection (Mirus Bio, MIR 2250). At 72 hours post-transfection ...
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No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Ling Wei, et al.,
bioRxiv - Systems Biology 2023
Quote:
... Cell lysates were clarified in filter plates (Pall Cat# 8075) at 3500 rpm for 30 minutes ...
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No products found
because this supplier's products are not listed.
Claudio Schmidli, et al.,
bioRxiv - Biophysics 2019
Quote:
... HeLa cell lysate (human origin, Enzo Life Sciences, BML-SW8750, Switzerland) was incubated in the presence of 0.7 mM of biotinylated Fabs at 4 °C for 5 h ...
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No products found
because this supplier's products are not listed.
Austin M. Stults, Gregory A. Smith,
bioRxiv - Microbiology 2019
Quote:
... HSV-1 strains were produced by electroporation of infectious clones into Vero cells using an ECM630 electroporation system (BTX Instrument Division, Harvard Apparatus). Cells were pulsed once with the following settings ...
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No products found
because this supplier's products are not listed.
Gonca Bayraktar, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Cell lysate extracts were incubated with the anti-GFP microbeads (Miltenyi Biotec GmbH, Gladbach, Germany) for 1h ...
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No products found
because this supplier's products are not listed.
Ethan C.M. Thomas, et al.,
bioRxiv - Microbiology 2022
Quote:
... Vero cells growing on 35mm glass-bottom dishes (MatTek, Ashland, MA) were transfected using X-treme GENE HP DNA transfection reagent (Roche ...
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No products found
because this supplier's products are not listed.
Enakshi Roy, Wen Shi, Bin Duan, St Patrick Reid,
bioRxiv - Microbiology 2020
Quote:
... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
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No products found
because this supplier's products are not listed.
Muriel E. Babey, et al.,
bioRxiv - Neuroscience 2024
Quote:
... with 10% human platelet-derived lysate (Stem Cell Technologies, Cat#06960) and 1% Penicillin-Streptomycin solution (Pen-Strep ...
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No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... Infectivity index was quantified by the ratio of green (virus infected cells) and red (total cells) fluorescence using Image Studio Lite software (LI-COR).
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No products found
because this supplier's products are not listed.
Priyanka Dutta Gupta, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... the cells were harvested and cell lysates processed using recommended buffers (cGAMP detection kit, Cayman Chemical) and then used for incubation with anti-cGAMP antibody and detection conjugate for 2 hours or overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Yilin Wang, et al.,
bioRxiv - Immunology 2021
Quote:
... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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No products found
because this supplier's products are not listed.
Jeffrey R. Johnson, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel) and proteins were transferred to nitrocellulose membranes ...
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No products found
because this supplier's products are not listed.
Pavlo Gilchuk, et al.,
bioRxiv - Immunology 2020
Quote:
A modified protocol using a commercially available human anti-zika virus Envelope protein (ZIKV-Env) IgG kit (Alpha Diagnostics International) was used to quantify ZIKV-117 mAb levels in NHP serum samples ...
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No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
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No products found
because this supplier's products are not listed.
Eul Sik Jung, et al.,
bioRxiv - Neuroscience 2020
Quote:
Cell lysates were prepared using RIPA buffer (Rockland Immunochemicals ...
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No products found
because this supplier's products are not listed.
Biao Zhou, et al.,
bioRxiv - Microbiology 2021
Quote:
... The cell-cell fusion between Vero-E6 TMPRSS2 and HEK293T-Spike-GFP was then determined under a fluorescence microscope (Nikon ELIPSE) and the images of randomly selected region were captured using 4× and 10× objectives using the Nikon software.
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No products found
because this supplier's products are not listed.
Kirsten E. L. Garner, et al.,
bioRxiv - Cell Biology 2022
Quote:
... S4 E and S5A) and Vero cells (Fig. S5 B) were imaged using an Olympus BX60 or BX50 microscope (Olympus Keymed) with a Plan apochromat 60X 1.4 N.A ...
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No products found
because this supplier's products are not listed.
Koen M.A. Dreijerink, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Total cell lysates were analyzed through immunoblotting using anti-menin (A300-105A, Bethyl) and anti-alpha tubulin (CP06 Calbiochem ...
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No products found
because this supplier's products are not listed.
Sarah W. Cai, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the P3 virus was used to infect Tni suspension insect cell culture (Expression Systems; Cat. 94-002S) for protein expression.
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No products found
because this supplier's products are not listed.
Nicholas M. Chesarino, Michael Emerman,
bioRxiv - Evolutionary Biology 2021
Quote:
... Cell lysate was measured for luciferase activity using a LUMIstar Omega microplate luminometer (BMG Labtech). Raw luciferase values were normalized to 2,000 mU RT activity for each virus ...
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No products found
because this supplier's products are not listed.
Meghan E. Wynne, et al.,
bioRxiv - Cell Biology 2022
Quote:
HAP1 or SH-SY5Y cell lysates or one nanogram of recombinant APOE (Novus Biologicals, 99158) were suspended in Buffer A + 0.5% Tx-100 and Laemmli sample buffer ...
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No products found
because this supplier's products are not listed.
Kyoko Tossell, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Stereotaxic virus injections were performed using an Angle Two apparatus (Leica) linked to a digital brain atlas (Leica Biosystems Richmond ...
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No products found
because this supplier's products are not listed.
Sara B. York, et al.,
bioRxiv - Microbiology 2021
Quote:
... Zika Envelope (EastCoast Bio; HM325), Zika capsid (GeneTex ...
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No products found
because this supplier's products are not listed.
Xueyan Zhang, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 400 μg total RNA from virus-infected Vero-E6 cells was incubated with an anti-m6A antibody (Synaptic Systems, Goettingen, Germany) or an IgG antibody in 300 μL IP buffer (150 mM NaCl ...
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No products found
because this supplier's products are not listed.
Tiansheng Li, et al.,
bioRxiv - Microbiology 2023
Quote:
Vero cells were pretreated with the indicated concentration of IFN-β (Peprotech; 300-02BC) for 18-20 h ...
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No products found
because this supplier's products are not listed.
Marco Grodzki, et al.,
bioRxiv - Microbiology 2021
Quote:
... 1): AGM Vero E6 cells were transduced with the newly generated Vervet sgRNA library and human HEK293T-hACE2 cells (Genecopoeia) were transduced with the Brunello sgRNA library ...
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No products found
because this supplier's products are not listed.
Hristo L. Svilenov, et al.,
bioRxiv - Immunology 2020
Quote:
... Vero E6 cells were incubated with a 1:500 dilution of an anti-dsRNA J2 antibody (Jena Bioscience) in PBS supplemented with 1% FCS at 4°C overnight with shaking ...
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No products found
because this supplier's products are not listed.
Sylvia Varland, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar) and denatured at 95°C for 5 min ...
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No products found
because this supplier's products are not listed.
Christopher M. Schafer, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Incubation of cell lysates with polyubiquitin-affinity beads (Calbiochem: #662200-1KIT) was carried out according to the manufacturer’s protocol followed by immunoblotting with an anti-ERG Ab (Santa Cruz ...
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No products found
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Maria C. Sterrett, et al.,
bioRxiv - Genetics 2022
Quote:
... Lysates were placed in a Mini Bead Beater 16 Cell Disrupter (Biospec) for 6 × 1 min at 25°C with ice submersion intervals of 1 minute between rounds ...
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No products found
because this supplier's products are not listed.
Lorène Gonnin, et al.,
bioRxiv - Microbiology 2023
Quote:
... the luciferase activities were determined for each cell lysate with an Infinite 200 Pro (Tecan, Männedorf, Switzerland) and normalised based on β-galactosidase (β-Gal ...
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12 well plate with tissue culture treated #1.5 glass-like polymer cover slip (0.175±0.010mm)....
Cat# P12-1.5P,
20/case, $221.00
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Kenneth H. Risner, et al.,
bioRxiv - Microbiology 2022
Quote:
... Vero cells were transferred to 35 mm Glass bottom dishes (Cellvis, D35-20-1-N) 24 hours after transfection ...
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No products found
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Jakob Kreye, et al.,
bioRxiv - Immunology 2020
Quote:
... Viruses were propagated on Vero E6 cells (ATCC CRL-1586) in minimal essential medium (MEM; PAN Biotech) supplemented with 10% fetal bovine serum (PAN Biotech ...
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No products found
because this supplier's products are not listed.
Javed Akhter, et al.,
bioRxiv - Microbiology 2020
Quote:
... Vero cells (ATCC©, CCL-81) were seeded at 20,000 cells per well on an E-Plate 16 (ACEA Biosciences, Inc., CA, USA) and incubated with the same media conditions as described previously at 36°C with 5% CO2 ...
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No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
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No products found
because this supplier's products are not listed.
Vikas A. Tillu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cleared cell lysates were prepared using a continuous flow cell disruptor (Constant Systems Limited, UK) at pressure range 25 – 30 kPsi with subsequent addition of 0.5 % w/v Triton X-100 (Cavin1 purification ...