-
No products found
because this supplier's products are not listed.
James S. Terry, et al.,
bioRxiv - Microbiology 2020
Quote:
... Stocks of virus were grown in Vero-E6 cells in DMEM (Gibco) supplemented with 10% fetal bovine serum (Atlas Biologicals ...
-
No products found
because this supplier's products are not listed.
Kyle Rosenke, et al.,
bioRxiv - Microbiology 2020
Quote:
... The virus was propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
-
No products found
because this supplier's products are not listed.
Madison S. Strine, et al.,
bioRxiv - Microbiology 2022
Quote:
... Diluted virus was added to 107 Vero-E6 cells grown in T175 (Corning) for three days ...
-
No products found
because this supplier's products are not listed.
Petrus Jansen van Vuren, et al.,
bioRxiv - Immunology 2022
Quote:
... Virus stocks were propagated and titrated in Vero E6 cells (American Type Culture Collection (ATCC), Manassas ...
-
No products found
because this supplier's products are not listed.
Jiahui Ding, et al.,
bioRxiv - Immunology 2021
Quote:
... ZIKA Viral RNA was isolated from Zika virus stock using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) to create a standard curve using serial 10-fold dilutions of ZIKV RNA ...
-
No products found
because this supplier's products are not listed.
Sara B. York, et al.,
bioRxiv - Microbiology 2021
Quote:
... Zika capsid (GeneTex; GTX133317), Zika prM (GeneTex;133305) ...
-
No products found
because this supplier's products are not listed.
Derek D.C. Ireland, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Primary antibodies used include: human polyclonal anti-Zika virus antibody (Kerafast EVU302, MA) or mouse anti-ZikV E protein mAb (Fitzgerald ...
-
No products found
because this supplier's products are not listed.
Kerri Basile, et al.,
bioRxiv - Microbiology 2023
Quote:
Vero C1008 (Vero 76, clone E6, Vero E6 [ECACC 85020206]) cells were seeded with DMEM (BE12-604F, Lonza Group AG (Basel, Switzerland)) from stocks in Costar□ 96-well clear tissue culture-treated flat bottom plates (353072 ...
-
No products found
because this supplier's products are not listed.
Stephen Graham, et al.,
bioRxiv - Immunology 2020
Quote:
... The virus-monoclonal antibody mixture was added (2 mL) to Vero cells in a 6-well plate (Greiner). After infecting the cells for 1 hour at 37°C ...
-
No products found
because this supplier's products are not listed.
Joshua Victor, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Miguel Ángel Cuesta-Geijo, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cytotoxicity in Vero cells was previously tested using CellTiter 96 (Promega). Vero cells were pre-treated with DMSO or increasing concentrations of U18666A drug ranging from 0.01-10 μM ...
-
No products found
because this supplier's products are not listed.
Boris Bonaventure, et al.,
bioRxiv - Microbiology 2021
Quote:
... Vero E6 cells (Merck) were a gift from Christine Chable (CEMIPAI ...
-
No products found
because this supplier's products are not listed.
Nick Platt, et al.,
bioRxiv - Immunology 2022
Quote:
Cell lysates were prepared using cell lysis buffer (Cell Signaling), incubated on ice for 60 min and spun at 4°C at 13K to remove insoluble material ...
-
No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
-
No products found
because this supplier's products are not listed.
Jin Wei, et al.,
bioRxiv - Genetics 2020
Quote:
... Cas9 activity was assessed by transducing parental Vero-E6 or Vero-E6-Cas9 cells with pXPR_047 (Addgene 107645), which expresses eGFP and an sgRNA targeting eGFP (72) ...
-
No products found
because this supplier's products are not listed.
N.D. Carvalho, et al.,
bioRxiv - Microbiology 2022
Quote:
Total RNA was extracted from 200μL of VERO cells culture infected by herpes virus (treated or not with hemolymph) using the MagNA Pure extractor (Roche, Basel, Switzerland). The Herpes simplex virus quantitative real time PCR assay was performed was described by Read et al. ...
-
No products found
because this supplier's products are not listed.
Qian Wang, et al.,
bioRxiv - Microbiology 2023
Quote:
... cell and virus particle lysates were treated with PNGase F and Endo Hf (New England Biolabs) following the manufacturer’s protocol for 1 hour at 37°C prior to Western blotting.
-
No products found
because this supplier's products are not listed.
Niraj Mishra, et al.,
bioRxiv - Microbiology 2019
Quote:
... Overnight UV-irradiation was performed to inactivate the virus in the cell lysate preparations and large debris was removed via filtering through 70 μm cell strainers (BD Biosciences).
-
No products found
because this supplier's products are not listed.
Wai-Lok Yau, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Viral stocks were propagated in Vero B4 cells or A549 MAVS knockout cells (Abcam, cat no. ab282354). LGTV strain TP21 (kindly provided by Gerhard Dobler ...
-
No products found
because this supplier's products are not listed.
Jonathan Burnie, et al.,
bioRxiv - Microbiology 2021
Quote:
The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
-
No products found
because this supplier's products are not listed.
Arthur Flohr Svendsen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Virus was produced by transfecting Platinum-E retroviral packaging cells (Cell Biolabs) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Mathias Cobbaut, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
-
No products found
because this supplier's products are not listed.
Richard J Smeyne, et al.,
bioRxiv - Neuroscience 2022
Quote:
... was propagated in Vero cells using a 10-30% sucrose gradient in an ultracentrifuge (Beckman L8-55), and the yield titrated using the classic Reed and Muench formula 20 ...
-
No products found
because this supplier's products are not listed.
Rahima Ziane, Alain Camasses, Marta Radman-Livaja,
bioRxiv - Molecular Biology 2020
Quote:
... Zirconium beads were removed from the cell lysate by centrifugation and the entire cell lysate was subject to sonication using the Bioruptor-Pico (Diagenode) for 3×10 cycles of 30 seconds ON/OFF each resulting in a final median size of chromatin fragments of 200 bp ...
-
No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
-
No products found
because this supplier's products are not listed.
Wyler Emanuel, et al.,
bioRxiv - Systems Biology 2020
Quote:
... virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators (Sartorius Stedim Biotech). Virus stocks were stored at −80°C ...
-
No products found
because this supplier's products are not listed.
Dirk Jochmans, et al.,
bioRxiv - Microbiology 2022
Quote:
... Passage 1 virus stocks were further produced in Vero TMPRSS2 cells and the presence of mutations was confirmed by Illumina sequencing ...
-
No products found
because this supplier's products are not listed.
Drishya Kurup, et al.,
bioRxiv - Microbiology 2020
Quote:
A codon optimized Zika prME-NS1 gene (strain PRVABC59 Asian 2015; GenBank: ANW07476.1) was gene synthesized by GenScript. The recombinant ZIKV-NS1 was constructed as published previously (13) ...
-
No products found
because this supplier's products are not listed.
Erick X. Pérez-Guzmán, et al.,
bioRxiv - Microbiology 2019
Quote:
DENV titrations by plaque assay were performed seeding Vero-81 (∼8.5×104 cells /well) in flat bottom 24-well cell culture well plates (Eppendorf) in supplemented DMEM the day before ...
-
No products found
because this supplier's products are not listed.
Hongyun Tong, et al.,
bioRxiv - Genetics 2021
Quote:
... Pulldowns of the GFP-fusion proteins were performed by incubating tissue homogenate (7-day-old seedlings) or cell lysate (CHO cell lysate) with agarose beads that conjugated with GFP antibodies (GFP Nano-Trap, Chromotek). After incubations of 4-24 hours with gentle inverting ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Sung-Eun Kim, et al.,
bioRxiv - Genetics 2024
Quote:
... The cell lysates were immunoprecipitated with HA (Santa Cruz sc-7392 ...
-
No products found
because this supplier's products are not listed.
Nikita Shiliaev, et al.,
bioRxiv - Microbiology 2021
Quote:
... Vero E6 and Huh7.5 cells with PiggyBAC/ACE2 and integrase-encoding plasmids (System Biosciences, Inc.) using Lipofectamine 3000 ...
-
No products found
because this supplier's products are not listed.
Mandy Boontanrart, et al.,
bioRxiv - Genetics 2020
Quote:
... Cell lysate concentrations were measured using Bradford reagent (VWR) or BCA assay (ThermoFisher) ...
-
No products found
because this supplier's products are not listed.
Chen-xi Zhang, et al.,
bioRxiv - Immunology 2020
Quote:
The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
-
No products found
because this supplier's products are not listed.
Irene González-Domínguez, et al.,
bioRxiv - Immunology 2022
Quote:
... VERO-TMPRSS2 cells (BPS Biosciences, #78081) were maintained in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Siyuan Hao, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus preparation or supernatants of virus-infected cells using a viral RNA extraction kit (Quick-RNA Viral Kit, R1035, Zymo Research) by following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Yuko Okemoto-Nakamura, et al.,
bioRxiv - Microbiology 2020
Quote:
... Full-length Vero-PVR1 and Vero-PVR2 cDNA was amplified with the Easy-A High Fidelity PCR Cloning Enzyme (Agilent Technologies, CA, USA), and cloned into T-vector pMD20 (Takara Bio Inc.) ...
-
No products found
because this supplier's products are not listed.
Erin R. Scheidemann, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... CTSD for mouse cell lysates (#MAB1123-SP) and CTSA for mouse cell lysates (AF1029-SP) were bought from R&D Systems.
-
No products found
because this supplier's products are not listed.
Longping V. Tse, et al.,
bioRxiv - Microbiology 2021
Quote:
... into Vero cell using PEI Max (MW 40,000) (Polysciences) and selected with 2.5 µg/ml Puromycin (Gibco) ...
-
No products found
because this supplier's products are not listed.
Emanuele Andreano, et al.,
bioRxiv - Immunology 2020
Quote:
... sub-confluent Vero E6 cell monolayers were prepared in T175 flasks (Sarstedt) containing supplemented D-MEM high glucose medium ...
-
No products found
because this supplier's products are not listed.
Susie S. Cha, Mark E. Bucklin, Xue Han,
bioRxiv - Bioengineering 2020
Quote:
... The micropipette was then frontloaded with virus or cells using a controller (WPI, Sys-Micro4). In general ...
-
No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
-
No products found
because this supplier's products are not listed.
Jessie J.-Y. Chang, et al.,
bioRxiv - Immunology 2023
Quote:
... propagation of the virus was carried out in Vero (African green monkey kidney epithelial – ATCC: CCL-81) cells cultured in MEM (MP Biomedicals), supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington) ...
-
No products found
because this supplier's products are not listed.
Miguel Á. Muñoz-Alía, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
-
No products found
because this supplier's products are not listed.
Markus Hoffmann, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Vero-TMPRSS2 cells additionally received puromycin (0.5 μg/ml, Invivogen). A549 (human ...
-
No products found
because this supplier's products are not listed.
Zhihua Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
-
No products found
because this supplier's products are not listed.
Prashant Rajbhandari, et al.,
bioRxiv - Cell Biology 2019
Quote:
Whole cell lysate or tissue lysate was extracted using RIPA lysis buffer (Boston Bioproducts) supplemented with complete protease inhibitor cocktail (Roche) ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Callie P. Wigington, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...