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No products found
because this supplier's products are not listed.
Ellen F. Gregory, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 1.65µL of each of two lmn-1 crRNA per strain (30pmol/µl) was combined and added to 1µL of purified Cas9 protein (5µg/µl from PNA Bio, Inc). Following addition of 1µL of the dpy-10 ssODN (500ng/µL ...
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No products found
because this supplier's products are not listed.
Takanobu Tagawa, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virus pellet was resuspended to EBM2 (Lonza) media ...
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No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
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No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
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No products found
because this supplier's products are not listed.
Yang Yang, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus titers were measured using p24 enzyme-linked immunosorbent assays (ELISA) (R&D Systems, MN, USA). CD4+ Jurkat T cells were incubated with NL4-3 or NL4-3dNef viruses at MOI ...
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No products found
because this supplier's products are not listed.
Nicole D. Marino, et al.,
bioRxiv - Microbiology 2023
Quote:
... LbCas12a was detected using LbCpf1 (strain ND2006) mouse monoclonal antibody (Cell Signaling Technology, #91982S) at 1:2000 dilution ...
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No products found
because this supplier's products are not listed.
Tai L. Ng, et al.,
bioRxiv - Systems Biology 2021
Quote:
... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
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No products found
because this supplier's products are not listed.
Iktae Kim, et al.,
bioRxiv - Biophysics 2022
Quote:
The binding of surface-immobilized NS1 to p85β was measured at 25 °C using an Octet RED biolayer interferometry (Pall ForteBio). The N-terminal His6 and SUMO-tagged NS1 proteins were used for immobilization ...
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No products found
because this supplier's products are not listed.
Kuan-Hung Lin, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and the virus titer was measured based on the titer of the viral protein p24 with QuickTiterTM Lentivirus Titer Kit (Cell Biolabs) following the manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Taiichi Tsuyama, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Thirty-nine strains that belong to the core forty strains were first tested by using stereomicroscopic observations without dissection (MS5, Leica), and flies that appeared to display AFB defects and were hard to judge were further examined by subsequent dissection (Table 4) ...
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No products found
because this supplier's products are not listed.
Nir Joffe, et al.,
bioRxiv - Microbiology 2023
Quote:
... One algal strain was inoculated into the wells of a 12-well plate and the second strain into Thincert inserts (Greiner). Cell abundances and virus production were monitored for both strains located within each vessel ...
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
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No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
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No products found
because this supplier's products are not listed.
Xiaozhen Liu, et al.,
bioRxiv - Genetics 2022
Quote:
... and vesicular stomatitis virus G protein (VSV-G) plasmid using polyetherimide (PEI) (B600070, ProteinTech Group, Chicago, USA) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Gabrielle L. Turvey, et al.,
bioRxiv - Cell Biology 2023
Quote:
... At 48 h post-transfection the supernatant containing virus was harvested and filtered through a low-protein binding filter (0.45µm, Sarstedt) to remove HEK debris ...
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No products found
because this supplier's products are not listed.
Jogindra Naik, et al.,
bioRxiv - Plant Biology 2023
Quote:
... tumefaciens strain GV3101 (pMP90) (GoldBio) and subsequently infiltrated using a syringe into the abaxial side of N ...
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No products found
because this supplier's products are not listed.
Susannah Waxman, et al.,
bioRxiv - Bioengineering 2021
Quote:
... A 4x strain-free objective (UPLFLN 4XP, Olympus, Tokyo, Japan) was used for both FM and PLM ...
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No products found
because this supplier's products are not listed.
David M. Garcia, et al.,
bioRxiv - Molecular Biology 2020
Quote:
Yeast strains were cultured on either YPD agar or liquid (RPI) or SD-Ura (Sunrise Scientific ...
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No products found
because this supplier's products are not listed.
Aleksandra Luginina, et al.,
bioRxiv - Biophysics 2023
Quote:
Leishmania tarentolae cells of the strain T7-TR (Jena Bioscience, Germany) were transfected with the A2AAR expression plasmids linearized by the SwaI restriction enzyme ...
-
No products found
because this supplier's products are not listed.
Shumpei Horii, et al.,
bioRxiv - Microbiology 2022
Quote:
Avermectin production using strain MA-4680T was measured using an LC/MS spectra (AB Sciex TripleTOF 5600+ System ...
-
No products found
because this supplier's products are not listed.
Linkang Wang, et al.,
bioRxiv - Microbiology 2023
Quote:
... while the other bacterial strains were cultured in Luria Bertani (LB) medium (Solarbio, Beijing, China) at 37°C ...
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No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.
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No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
-
No products found
because this supplier's products are not listed.
Qiuli Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Sara B. York, et al.,
bioRxiv - Microbiology 2021
Quote:
... Zika Envelope (EastCoast Bio; HM325), Zika capsid (GeneTex ...
-
No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
-
No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The RBD-binding antibodies against the ancestral strain variant were depleted by using magnetic beads coated with the RBD fragment of ancestral strain SARS-CoV-2 (AcroBiosystems, MBS-K002). According to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Katherine S. Wetzel, et al.,
bioRxiv - Microbiology 2023
Quote:
... abscessus strains using a spectrophotometer (Tecan, infinite 200 PRO) until stationary phase was reached (2 days for M ...
-
No products found
because this supplier's products are not listed.
Emily A. Hemann, et al.,
bioRxiv - Immunology 2022
Quote:
... this virus/serum mixture is incubated with 0.5% chicken red blood cells (Rockland) and hemagglutination inhibition was measured after 30 minutes.
-
No products found
because this supplier's products are not listed.
Cawez Frédéric, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Strains were lysed by sonication with a Bioruptor Plus (Diagenode, Belgium) and centrifuged at 18000g in order to recover the bacterial content ...
-
No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...
-
No products found
because this supplier's products are not listed.
Chenyan Ma, et al.,
bioRxiv - Neuroscience 2023
Quote:
... starting at day 3 post virus injection (250 mg tamoxifen per kg of chow, Research Diets). Mice were gavaged with two additional doses of tamoxifen (250 mg/kg body weight ...
-
No products found
because this supplier's products are not listed.
Kai Bi, et al.,
bioRxiv - Microbiology 2020
Quote:
... The transgenic fungal strains were cultured on PDA amended with 100 μg/ml hygromycin B (Calbiochem) and/or 100 μg/ml Nourseothricin (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Richard J Mills, et al.,
bioRxiv - Cell Biology 2021
Quote:
... or 1 μg/ml LPS (from Escherichia coli strain 0127:B8, Sigma) (all Peprotech unless noted). Additional concentrations were performed for the dose-response curves for TNF ...
-
No products found
because this supplier's products are not listed.
Stefanie Krug, et al.,
bioRxiv - Immunology 2021
Quote:
... Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
David Gonzalez-Perez, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Proteins were separated by SDS-PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Richard S Tedder, et al.,
bioRxiv - Microbiology 2019
Quote:
Recombinant ZKV NS1 (rZKVNS1Ag) antigen expressed by baculovirus in insect cells (MyBioSource Inc, San Diego, USA) was used both to coat the solid-phase where appropriate and ...
-
No products found
because this supplier's products are not listed.
Robin Anger, et al.,
bioRxiv - Microbiology 2023
Quote:
... while strain BTH101 (Euromedex) – a non-reverting cya mutant – was used in BACTH assays29 ...
-
No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
-
No products found
because this supplier's products are not listed.
Christopher M. Hoel, et al.,
bioRxiv - Biophysics 2022
Quote:
... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
-
No products found
because this supplier's products are not listed.
Leena Hussein Bajrai, et al.,
bioRxiv - Microbiology 2021
Quote:
... the plates were analyzed by observing virus-induced CPE by light microscope (Nikon-ECLIPSE-Ti), and plaque formation was determined by crystal violet staining (C0775 ...
-
No products found
because this supplier's products are not listed.
Lei Zhang, José M Jiménez-Gómez,
bioRxiv - Plant Biology 2019
Quote:
... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...
-
No products found
because this supplier's products are not listed.
Pablo Castro-Córdova, et al.,
bioRxiv - Microbiology 2020
Quote:
... and protein concentration was quantified by BCA protein kit (RayBiotech USA). Next ...
-
No products found
because this supplier's products are not listed.
Hui Chen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...