-
No products found
because this supplier's products are not listed.
Sherif Abouelhadid, et al.,
bioRxiv - Microbiology 2021
Quote:
... coli strain EC100D pir+ (Cambio U.K.). The plasmid was then transformed into E ...
-
The E protein composes the majority of the virion surface and it is involved with replication,...
Cat# abx061381-1MG,
1 mg USD $2001.0
Ask
James C. Chamness, et al.,
bioRxiv - Plant Biology 2022
Quote:
... coli strain DB3.1 (ThermoFisher, discontinued; available from Abbexa, abx098865), and all other vectors were propagated in either NEB10β Competent or NEB Stable Competent cells (NEB C3019I ...
-
No products found
because this supplier's products are not listed.
Erliang Yuan, et al.,
bioRxiv - Developmental Biology 2022
Quote:
The pea aphid Acyrthosiphon pisum (strain: Ningxia Red) was originally collected from Medicago sativa in Ningxia Province and had been reared in the laboratory for over 8 years ...
-
No products found
because this supplier's products are not listed.
Taeyong Kwon, et al.,
bioRxiv - Microbiology 2022
Quote:
... The same amount of the virus was mixed with 45 µL of human saliva (Lee Biosolutions) or medium and transferred into a 2 mL tube or onto stainless steel in the 12-well plate ...
-
No products found
because this supplier's products are not listed.
Daniela Niemeyer, et al.,
bioRxiv - Microbiology 2021
Quote:
... and B.1.351 was assessed by a surrogate virus neutralization test (cPass Assay, Medac, Wedel, Germany) as described previously (Momsen Reincke et al. ...
-
No products found
because this supplier's products are not listed.
Susanne A. Wycislo, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Equivalent amounts of “light”-labeled control and “heavy”-labeled GFP–containing lysates or “light”-labeled control and “heavy”-labeled GFP–containing lysates were incubated (separately) with GFP-Trap agarose beads (Allele Biotechnology, San Diego, CA) for 30 min at 4°C ...
-
No products found
because this supplier's products are not listed.
Lisa-Marie Kuhl, et al.,
bioRxiv - Genetics 2020
Quote:
... Cell lysates were mixed on a VXR basic Vibrax (IKA) for 2 min at 1500 rpm ...
-
No products found
because this supplier's products are not listed.
Tiffany C. Taylor, et al.,
bioRxiv - Immunology 2022
Quote:
... tuberculosis strain H37Ra (DIFCO laboratories) and administered 100 ng pertussis toxin (List Biological Laboratories) i.p ...
-
No products found
because this supplier's products are not listed.
Meredith J. Sigman, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Clarified lysates were combined with 2 μL of AGO4 antibody (Agrisera) or 2uL of rabbit IgG as a mock IP (Cell Signaling Technology) ...
-
No products found
because this supplier's products are not listed.
Jie Dong, et al.,
bioRxiv - Plant Biology 2020
Quote:
... The lysate was filtered through a 30 μm mesh (Sysmex Partec). Propidium iodide (Sigma ...
-
No products found
because this supplier's products are not listed.
Anna Fortuny, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Lysates were homogenised with a tight Dounce homogeniser (DWK Life Sciences) and nuclei were collected by centrifugation ...
-
No products found
because this supplier's products are not listed.
Milène Vandal, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The lysate was incubated with 100 U/mL salt-active nuclease (Arcticzymes) at 37°C for 1 h and then centrifuged at 2000 xg for 15 min ...
-
No products found
because this supplier's products are not listed.
Emily S. Norton, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... lysates were clicked to 10 μM DBCO-S-S-PEG3-biotin (BroadPharm) for 6 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Richard J Mills, et al.,
bioRxiv - Cell Biology 2021
Quote:
... or 1 μg/ml LPS (from Escherichia coli strain 0127:B8, Sigma) (all Peprotech unless noted). Additional concentrations were performed for the dose-response curves for TNF ...
-
No products found
because this supplier's products are not listed.
Avital Licht-Murava, et al.,
bioRxiv - Neuroscience 2022
Quote:
... per ml culture medium at DIV 8 using Lipofectamine 3000 5 h before infection with vesicular stomatitis virus (VSV) at 100 MOI or with adenovirus-eGFP (Ad5CMV-eGFP, lot #ad3586, Viral Vector Core Facility, Carver College of Medicine ...
-
No products found
because this supplier's products are not listed.
Sara Al Rawi, et al.,
bioRxiv - Neuroscience 2021
Quote:
Fbxo7 was immunoprecipitated from cell lysates using 2μl of anti-Fbxo7 antibody (Aviva), or isotype matched control ...
-
No products found
because this supplier's products are not listed.
Vigneshwaran Venkatesan, et al.,
bioRxiv - Biochemistry 2022
Quote:
... the protein lysate was analysed for haemoglobin tetramer using G8 HPLC Analyzer (Tosoh). The globin chain analysis was performed using HPLC equipment with UV detector (Shimadzu ...
-
Cat# ZK312-1,
USD $795.0/mg
Ask
Milica Moskovljevic, et al.,
bioRxiv - Immunology 2024
Quote:
... Cells stimulated with either lysates of CMV-infected fibroblasts (Virusys, 10 μg/mL), overlapping Gag 15mer peptides (HIV-1 Gag peptide pool ...
-
No products found
because this supplier's products are not listed.
Adam S Hassan, et al.,
bioRxiv - Immunology 2019
Quote:
... Successfully transformed strains were identified using LB agar containing 50 μg/mL ampicillin (Wisent Bioproducts, St-Bruno, QC). Aliquots of each transformed strain were stored in LB with 15% glycerol at −80°C until used in experiments.
-
No products found
because this supplier's products are not listed.
Ashley S. Denney, et al.,
bioRxiv - Genetics 2021
Quote:
... The TH5 strain was propagated on rich or synthetic medium with 100 µg/mL dTMP (TCI America #TCT0845), or on minimal medium containing essential amino acids adenine ...
-
No products found
because this supplier's products are not listed.
Paola Munoz-Tello, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... non-infectious B31-e2 background strain (6) was grown in BSK-II medium supplemented with 6% (v/v) heat-inactivated normal rabbit serum (Atlanta Biologicals) at 33°C ...
-
No products found
because this supplier's products are not listed.
Stefan L Nordqvist, et al.,
bioRxiv - Microbiology 2020
Quote:
... The phenotype of the resulting strains was checked by agglutination with an O1 and Ogawa-specific monoclonal antibodies (Fitzgerald, United States)
-
No products found
because this supplier's products are not listed.
Tansy Vallintine, Christiaan van Ooij,
bioRxiv - Microbiology 2023
Quote:
... falciparum erythrocytic stage parasites of strain 3D7 were cultured in human erythrocytes (UK National Blood Transfusion Service and Cambridge Bioscience, UK) at 3% haematocrit and 37°C ...
-
No products found
because this supplier's products are not listed.
DaoFei Song, Lei Yin, Chang Wang, XiuYing Wen,
bioRxiv - Molecular Biology 2019
Quote:
... The protein concentration in tissue lysates was measured with a BCA protein assay kit (Boster, Wuhan, China) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Nicholas McCaul, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... IgM was immunoprecipitated from lysates or media samples with a goat anti-mouse IgM antibody (Southern Biotech). Immunoprecipitates were washed with lysis buffer and eluted from beads with reducing SDS-PAGE sample buffer and analyzed by SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Julieta Ramirez, et al.,
bioRxiv - Genomics 2023
Quote:
Whole cell lysates were separated on a 5% SDS/PAGE containing 20 μm Phos-tag (NARD Institute), followed by western blotting with anti-CTCF antibody.
-
No products found
because this supplier's products are not listed.
Kelly T. Rios, et al.,
bioRxiv - Microbiology 2024
Quote:
The gametocyte and zygote lysates were mechanically lysed with a 1 mL Dounce homogenizer (Wheaton, Cat# 357538) for one minute on ice with a tight pestle following chemical lysis ...
-
No products found
because this supplier's products are not listed.
Ayush Saurabh, et al.,
bioRxiv - Biophysics 2023
Quote:
HeLa cells (Kyto strain) were grown in 60 mm glass petri dishes on Poly-d-lysine coated 40 mm #1.5 coverslips (Bioptechs, 40-1313-03192) for a minimum of 48 h in DMEM media (ATCC 30-2002 ...
-
No products found
because this supplier's products are not listed.
Matthew L. Turner, et al.,
bioRxiv - Microbiology 2019
Quote:
... Potassium was measured in the cleared lysates using a Jenway PFP7 flame photometer (Cole-Parmer, Stone, Staffordshire, UK).
-
No products found
because this supplier's products are not listed.
Michael J. D. Daniels, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 25 μL lysate was combined with 175 μL cathepsin probe L (Z-Phe-Arg-AMC, 45 μM, Bachem) or C (H-Gly-Phe-AMC ...
-
No products found
because this supplier's products are not listed.
Julia H. Kemis, et al.,
bioRxiv - Systems Biology 2019
Quote:
40 μL of DO plasma collected at sacrifice (30 μL used for founder strains) were aliquoted into a tube with 10 μL SPLASH Lipidomix internal standard mixture (Avanti Polar Lipids, Inc.). Protein was precipitated by addition of 215 μL MeOH ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
... the clarified lysate was concentrated by tangential flow filtration at 5 L/min/m2 using a KR2i KrosFlo (Repligen) with Pellicon 2 mini cassettes (300 kDa ...
-
No products found
Liliana M. Sanmarco, et al.,
bioRxiv - Immunology 2023
Quote:
Pyruvate levels were quantified in BMDC lysates after 1h stimulation using EnzyChrom pyruvate assay kit (EPYR-100, BioAssay Systems), followed the procedure suggested by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Jose L. Nieto-Torres, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The in vitro kinase reaction mixtures were incubated with 350 μl clarified lysate of HeLa LC3B-KO cells (8×105 cell equivalents, prepared as described above) and glutathione-sepharose beads (Bioworld) for 3 h at 4°C on a rotator ...
-
No products found
because this supplier's products are not listed.
Nitchakarn Kaokhum, et al.,
bioRxiv - Biochemistry 2022
Quote:
DUB activity in cartilage tissue lysates were measured using ubiquitin-rhodamine(110)-glycine quenched substrate (Boston Biochem, cat No U-555). In the presence of active DUBS ...
-
No products found
because this supplier's products are not listed.
Vi Pham, et al.,
bioRxiv - Cell Biology 2023
Quote:
... SGSH activity was measured from whole cell lysates following a two-step protocol [25] and using 4MU-αGlcNS (Chem-Impex Int’l Inc, Cat #30694) as fluorogenic substrate and with 4-methylumbelliferone (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Trevor J. Hancock, et al.,
bioRxiv - Immunology 2022
Quote:
... as well as TGEV (transmissible gastroenteritis virus) (VMRD, Pullman, WA, USA). Normal cat serum was purchased from Jackson ImmunoResearch (West Grove ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... goat anti- xenotropic MLV virus antibody ABIN457298 (1:1000, antibodies-online); mouse anti- MLV gag ab100970 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The RBD-binding antibodies against the ancestral strain variant were depleted by using magnetic beads coated with the RBD fragment of ancestral strain SARS-CoV-2 (AcroBiosystems, MBS-K002). According to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Jason L. Cantera, et al.,
bioRxiv - Microbiology 2021
Quote:
... tuberculosis strain Aoyama-B was procured from Nacalai USA, Inc ...
-
No products found
because this supplier's products are not listed.
Zhenming Jin, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and Anti-virus Drug Library (Shanghai Institute for Advanced Immunochemical Studies, SIAIS), which includes ∼10,000 compounds ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
... A fixed concentration of SARS-CoV-2 GFP pseudotyped virus (BPS Biosciences) was added and the plate incubated for 60 minutes at 37°C and 5% CO2 ...
-
No products found
because this supplier's products are not listed.
M. Águila Ruiz-Sola, et al.,
bioRxiv - Plant Biology 2023
Quote:
... icl-C (icl strain complemented with the WT ICL gene), dum11 (defective in defective in ubiquinol cytochrome c oxidoreductase of the respiratory complex III ...
-
No products found
because this supplier's products are not listed.
Katharina Kases, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and anti- ZC3H11A (Atlas Antibodies, heavy lysate) antibodies in Protein LoBind tubes (Eppendorf) ...
-
No products found
Yuanyuan He, et al.,
bioRxiv - Microbiology 2023
Quote:
Imaging plates for virus quantification were prepared by coating coverslip-bottom wells (Cellvis) with 0.18 mg/ml BSA-biotin in PBS overnight at 4 °C ...
-
No products found
Leander Rohr, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Proteins were transiently expressed using the AGL1 Agrobacterium tumefaciens strain (Lifeasible), as previously described (Hecker et al. ...
-
No products found
because this supplier's products are not listed.
Luis E. Martinetti, et al.,
bioRxiv - Neuroscience 2021
Quote:
... we used a virus-retrobead or saline-retrobead mixture (red RetroBeads, Lumafluor, Cat# R180). When comparing different AAV serotypes ...
-
No products found
because this supplier's products are not listed.
Sheng Xiao, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected using a manual volume displacement injector (Narishige International USA, MMO-220A) connected to a glass pipette (Drummond Scientific ...
-
No products found
because this supplier's products are not listed.
Cinzia Klemm, Gudjon Olafsson, Peter H Thorpe,
bioRxiv - Cell Biology 2023
Quote:
... All plasmids and strains were validated by Sanger sequencing (GENEWIZ Brooks Life Science, UK).