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No products found
because this supplier's products are not listed.
Javed Akhter, et al.,
bioRxiv - Microbiology 2020
Quote:
... The recombinant Envelope protein was obtained from MyBioSource (Cat#MBS8309649). All other reagents were from Sigma Aldrich.
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No products found
because this supplier's products are not listed.
Bin Zhou, et al.,
bioRxiv - Microbiology 2020
Quote:
... S proteins with Avi-tag were pre-biotinylated using BirA biotin-protein ligase standard reaction kit (Avidity). 25 nM S-614D or 15 nM S-614G in 10X kinetic buffer (ForteBio ...
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No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
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No products found
because this supplier's products are not listed.
Amanda M. Travis, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and C-terminal MYC tag was placed in front of human NPHP3 (GeneCopoeia GC-H2370). All mutations to replace the lipidated cysteine with alanine were made using PCR mutagenesis (Weiner et al. ...
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No products found
because this supplier's products are not listed.
Satoshi Ninagawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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No products found
because this supplier's products are not listed.
Gary Baisa, et al.,
bioRxiv - Immunology 2020
Quote:
... an RBD-Fc fragment (319-541) with a C-terminal human Fc-tag was purchased from BPS Bioscience (San Diego, CA). Recombinant biotinylated ACE2 was purchased from Acro Biosystems (Newark ...
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No products found
because this supplier's products are not listed.
Haizhang Chen, et al.,
bioRxiv - Immunology 2020
Quote:
... or a PE-TexasRed-conjugated human IgG-Fc fragment (Rockland) for 1h at 4°C in PBS/3%FCS ...
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No products found
because this supplier's products are not listed.
Fangzhou Lou, et al.,
bioRxiv - Immunology 2020
Quote:
... Normal Human Epidermal Keratinocytes (NHEKs; Lifeline Cell Technology, cat. FC-0007) stored in liquid nitrogen were defrosted and cultured in serum-free basal medium with growth factors (Lifeline Cell Technology ...
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No products found
because this supplier's products are not listed.
CL Esposito, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant Human DNMT1 with an N-terminal GST tag was purchased from Active Motif and Pierce Glutathione Magnetic Beads (Thermo Scientific ...
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No products found
because this supplier's products are not listed.
Jiahao Ma, et al.,
bioRxiv - Biophysics 2020
Quote:
... ACE2-Fc (10 μg/mL) was immobilized on Protein A (ProA) biosensors (Pall). Real-time receptor binding curves were obtained by applying the sensor in a two-fold serial dilutions of S-Trimer from 22.5-36 μg/mL in PBS ...
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No products found
because this supplier's products are not listed.
Krisztina Ötvös, et al.,
bioRxiv - Plant Biology 2019
Quote:
For BiFC assays Yellow Fluorescent Proteins (YFP) were visualized using a Leica TCS-SP2 laser scanning confocal microscope (LSM) (Leica Microsystems, Heidelberg, Germany) with a 63×1.4 NA oil-objective lens and processed using Leica confocal software v.2.61 about 16 h post-transformation ...
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No products found
because this supplier's products are not listed.
Carolin Gerke, et al.,
bioRxiv - Microbiology 2023
Quote:
... FCS (PAN Biotech), 1 % (v/v ...
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Recombinant Human MADCAM1(19-317 aa) fused with Fc fragment of Human IgG was expressed in HEK293.
Cat# MADCAM1-3983H,
50ug , USD $298
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Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Recombinant protein of human DSG2 tagged with IgG Fc domain (Fc) (Creative Biomart; #DSG2-1601H) and N-CAD-Fc (Sino Biological ...
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No products found
because this supplier's products are not listed.
Motokazu Uchigashima, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Tag-blue fluorescent protein (Tag-BFP, Evrogen), Nlgn3Δ ...
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No products found
because this supplier's products are not listed.
Jennifer L. Reedy, et al.,
bioRxiv - Immunology 2022
Quote:
... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...
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No products found
because this supplier's products are not listed.
Alexandra Paul, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The envelope vector expressing VSV-G (vesicular stomatitis virus glycoprotein) was obtained from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). To generate the expression plasmid for D614G mutant of SARS-CoV-2 Spike (S ...
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No products found
because this supplier's products are not listed.
Veronica Locher, et al.,
bioRxiv - Immunology 2022
Quote:
Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
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No products found
because this supplier's products are not listed.
Joachim S. Grimstvedt, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and a red fluorescent protein tag (tdTomato; ChromoTek, 5f8-100, 1:500) expressed by the rabies virus ...
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No products found
because this supplier's products are not listed.
Berislav Bošnjak, et al.,
bioRxiv - Immunology 2022
Quote:
... FCS files were analyzed using FCS Express V7 (De Novo Software) or FlowJo V10 (BD).
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No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
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No products found
because this supplier's products are not listed.
David Andruszewski, et al.,
bioRxiv - Neuroscience 2023
Quote:
... resuspended in PBS/FCS supplemented with Fc-block (BioXCell) and incubated for 15min on ice ...
-
No products found
because this supplier's products are not listed.
Harman Malhi, et al.,
bioRxiv - Immunology 2022
Quote:
... Anti-Human Fc biosensors (ForteBio, catalog #18-0015) were submerged in wells of black 96-well microplates (Greiner, catalog #655209) containing 250 μL of kinetics buffer (PBS ...
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No products found
because this supplier's products are not listed.
Andrés Guillén Samander, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The Twin-Strep tag and Strep-Tactin XT protein fluorescently conjugated with DY-649 were purchased from IBA Lifesciences.
-
No products found
because this supplier's products are not listed.
Carmen Campos-Silva, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Cytomics FC 500 (Beckman Coulter) or CytoFLEX (Beckman Coulter ...
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No products found
because this supplier's products are not listed.
Jinchao Chen, et al.,
bioRxiv - Plant Biology 2023
Quote:
... GST-tag Protein Purification Kit (Glutathione Sepharose, BioVision) and MBP-tag Protein Purification Kit (Dextrin Beads ...
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No products found
because this supplier's products are not listed.
Dipan C. Patel, et al.,
bioRxiv - Neuroscience 2023
Quote:
... fluorescent dye Lucifer yellow (25573, Cayman Chemical) was added to intracellular solution (0.4 mg/ml ...
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No products found
because this supplier's products are not listed.
Siti Naqiah Amrun, et al.,
bioRxiv - Immunology 2019
Quote:
... virus inoculum was removed and replaced with IMDM with 10% human serum (HS; Innovative Research Inc). Cells were incubated at 37°C and harvested at time-points 0 ...
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No products found
because this supplier's products are not listed.
E Chuntharpursat-Bon, et al.,
bioRxiv - Cell Biology 2019
Quote:
... perfusion set (yellow/green) and fluidics unit (Ibidi). Cells were exposed to shear stress of 10 dyn.cm−2 for 24 hr ...
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No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... human recombinant GST-fusion ERK3 protein (SignalChem) and E.coli purified ARP3 ...
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No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Yu-Feng Chien, et al.,
bioRxiv - Bioengineering 2020
Quote:
... a TAG lens (TAG lens 2.5β, TAG Optics) was placed directly on top of an objective (UPLFLN 10XP, Olympus) to enable high-speed axial scan of the focus ...
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No products found
because this supplier's products are not listed.
Alexander W. Justin, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Green fluorescent protein (GFP) and Red Fluorescent Protein (RFP) human umbilical vein endothelial cells (HUVECs, Promocell), normal human lung fibroblasts (NHLFs ...
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No products found
because this supplier's products are not listed.
Matthew Gagne, et al.,
bioRxiv - Immunology 2022
Quote:
... SARS-CoV-2 S-pseudotyped virus was prepared by transfection in 293T/17 cells (human embryonic kidney cells in origin; obtained from American Type Culture Collection, #CRL-11268) using a lentivirus backbone vector ...
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No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
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No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
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No products found
because this supplier's products are not listed.
Erik Procko,
bioRxiv - Biochemistry 2020
Quote:
Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
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No products found
because this supplier's products are not listed.
Jieqiong Qu, et al.,
bioRxiv - Microbiology 2023
Quote:
... CHIKV virus stock (5 × 108 TCID50/mL) and full human blood (Sanquin) was 1:2 mixed to make the infectious blood meal with a final virus titer of 1.7× 108 TCID50/ml ...
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No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
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No products found
because this supplier's products are not listed.
Young Bong Choi, et al.,
bioRxiv - Immunology 2021
Quote:
Purified recombinant human TAX1BP1 protein (catalog # P01; Abnova) was incubated with purified GST-tagged IKKi (catalog # PV4875 ...
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No products found
because this supplier's products are not listed.
Patrik Risteski, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and human anti-centromere protein antibody (Antibodies Incorporated). The secondary antibodies used were donkey anti-mouse IgG-Alexa Fluor 488 (Abcam) ...
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
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No products found
Konstantina Nikolatou, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... V5-Tag (ABM, G189). Following three PFS washes ...
-
No products found
because this supplier's products are not listed.
Julianne B. Riggs, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were subjected to Fc receptor blocking using Fc Shield (Tonbo Biosciences) for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Imke M.E. Schuurmans, et al.,
bioRxiv - Neuroscience 2024
Quote:
... RevitaCell™ and 2.5% AM FCS (ScienCell®; 0010). Then medium of the neurons was refreshed with neurobasal medium supplemented with B-27 ...
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No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Purified human complement proteins (4 µg/mL, Complement Technology) were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL ...
-
No products found
because this supplier's products are not listed.
Juan Jauregui-Lozano, et al.,
bioRxiv - Genomics 2021
Quote:
CUT&Tag was performed using CUTANA™ CUT&Tag reagents (Epicypher, Durham NC ...
-
No products found
because this supplier's products are not listed.
Kensuke Ninomiya, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... All MS/MS spectra were searched against protein sequences of the RefSeq (NCBI) human protein database (RDB) using the Protein Pilot software package (AB Sciex), and decoy sequences were then selected with a false discovery rate <1%.
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No products found
because this supplier's products are not listed.
Jamie C. Moffa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
No products found
because this supplier's products are not listed.
Andrew B Janowski, et al.,
bioRxiv - Microbiology 2022
Quote:
... the virus RNA was isolated from the virus stocks using Direct-zol RNA MicroPrep (Zymo research) and used for RT-PCR and Sanger sequencing of the entire virus genome.
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No products found
because this supplier's products are not listed.
Yi-Sook Kim, et al.,
bioRxiv - Microbiology 2020
Quote:
... into a 200 μL yellow tip with C18 Empore disk membranes (3M, Bracknell, UK) at the bottom ...