-
No products found
because this supplier's products are not listed.
Hirak Saxena, et al.,
bioRxiv - Microbiology 2023
Quote:
All strains were grown in 2YT media (16 g/L tryptone, 10 g/L yeast extract, 5 g/L NaCl, BioShop Canada). NEB® Stable E ...
-
No products found
because this supplier's products are not listed.
Rei Matsuoka, et al.,
bioRxiv - Biophysics 2021
Quote:
... Yeast PI solubilized in chloroform (Larodan) was dried using a rotary evaporator (Hei-Vap Core ...
-
No products found
because this supplier's products are not listed.
Ute Kothe, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... genomic DNA from yeasts cells was extracted by using Geneaid-Presto Mini gDNA Yeast Kit from FroggaBio Scientific Solutions following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Marisa Oliveira, et al.,
bioRxiv - Immunology 2020
Quote:
... and chicken interferon alpha (Yeast-derived Recombinant Protein, Kingfisher Biotech, Inc) were diluted in nuclease-free water (Ambion ...
-
No products found
because this supplier's products are not listed.
Arthur T. Molines, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Yeast cells were imaged with a 60x TIRF objective (Nikon, MRD01691) on a Nikon TI- E equipped with a Nikon TIRF system and a SCMOS camera (Andor ...
-
No products found
because this supplier's products are not listed.
G. Ozan Bozdag, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
We fixed snowflake yeast in 2% formaldehyde (fresh from paraformaldehyde (EMS)) containing 2 mM CaCl2 ...
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No products found
because this supplier's products are not listed.
Ravikanth Maddipati, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 25 μg/mL bovine pituitary extract (Gemini Bio-Products), 5 nmol/L 3,3’,5-triiodo-L-thyronine (Sigma) ...
-
No products found
because this supplier's products are not listed.
Natsumi Takei, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Then the extracts were diluted with Detergent Solution (MBL International) and centrifuged at 3,000 g for 3 min at 4°C ...
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No products found
because this supplier's products are not listed.
Rafael Muñoz-Tamayo, et al.,
bioRxiv - Microbiology 2019
Quote:
... and extracts run on a FlashGel System (Lonza, Rockland, Inc) to check integrity.
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No products found
because this supplier's products are not listed.
Bastien Bennetot, et al.,
bioRxiv - Genetics 2023
Quote:
... Genomic DNA was extracted using the Fungi/Yeast Genomic DNA Isolation Kit (Norgen Biotek Corp.) with the following modifications ...
-
No products found
because this supplier's products are not listed.
Lorenzo Tattini, et al.,
bioRxiv - Genomics 2019
Quote:
... Assemblies can be downloaded from the “Population-level Yeast Reference Genomes” website (https://yjx1217.github.io/Yeast_PacBio_2016/welcome/). Duplicates are removed by SAMtools 1.3.1 (using HTSlib 1.3.1) ...
-
No products found
because this supplier's products are not listed.
Sang-Gyun Kang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Extract purity was determined by probing with anti-β-tubulin pAb (NB600-936, Novus Biologicals), and anti-Lamin B1 pAb (ab16048 ...
-
No products found
because this supplier's products are not listed.
Yuma Nishikawa, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... Protein was extracted from the cells using E.coli/Yeast Protein Extraction Buffer (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), and the lysate fraction was used for analysis.
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No products found
Sumit Mukherjee, et al.,
bioRxiv - Microbiology 2020
Quote:
... SOD activity in whole cell extracts was measured using the EnzyChrome superoxide dismutase assay kit (BioAssay Systems) according to the manufacturer’s protocol.
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No products found
because this supplier's products are not listed.
Patrick O. Sorensen, et al.,
bioRxiv - Microbiology 2020
Quote:
... NO3- concentration in the 0.5M K2SO4 extracts was measured colorimetrically using a Versamax microplate spectrophotometer (Molecular Devices) using a modified Greiss reaction protocol (Doane and Horwath 2003) ...
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No products found
because this supplier's products are not listed.
Vivienne Woo, et al.,
bioRxiv - Microbiology 2021
Quote:
... and nuclear extracts were washed in TE 0.1% SDS with protease inhibitors and sonicated using a S220 Focused-ultrasonicator (Covaris). Prior to immunoprecipitation ...
-
No products found
because this supplier's products are not listed.
Yue Wu, et al.,
bioRxiv - Immunology 2022
Quote:
Denature of Protein of both the cytoplasmic extract and nuclear extract was performed utilizing 4X Protein Sample Loading Buffer (LI-COR) with β-mercaptoethanol ...
-
No products found
because this supplier's products are not listed.
Jared Feldman, et al.,
bioRxiv - Immunology 2021
Quote:
... yeast was stained with donkey anti-chicken IgY AF488 (Jackson ImmunoResearch). Two gates were drawn for cells with improved RBD binding over parental clones ...
-
No products found
because this supplier's products are not listed.
Tessa E. Steenwinkel, et al.,
bioRxiv - Physiology 2022
Quote:
... we measured the yeast powder using micro curettes (Fine Science Tools). The smallest available micro cuvette featured a tip diameter of 0.5 mm (#10080-05) ...
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No products found
because this supplier's products are not listed.
Pedro Henrique Bürgel, et al.,
bioRxiv - Immunology 2019
Quote:
... Yeast cells were then incubated with 0.01% Uvitex2B (a chitin marker; Polysciences) and mAb 18B7 (10 µg/ml ...
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No products found
because this supplier's products are not listed.
Rakesh Chatrikhi, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The in vitro splicing reaction used HeLa nuclear extract (ProteinOne) with a similar protocol as described56 ...
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No products found
because this supplier's products are not listed.
Elisa Violeta Bertini, et al.,
bioRxiv - Microbiology 2021
Quote:
... Protein extracts were obtained using the YPX extraction kit (EXPEDEON), and concentrations were determined with the QuantiPro BCA (SigmaAldrich) ...
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No products found
because this supplier's products are not listed.
Friederike Roger, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The yeast cells were kept in a heated perfusion chamber (FCS2, Bioptechs Inc.) at 28 °C to avoid heat-induced stress responses ...
-
No products found
because this supplier's products are not listed.
Christophe Chapard, et al.,
bioRxiv - Genetics 2023
Quote:
... Yeast cells were synchronized in G1 by adding α-factor (Proteogenix, WY-13) in the media every 30 min during 2h30 (1 μg/mL final) ...
-
No products found
because this supplier's products are not listed.
Vivienne Woo, et al.,
bioRxiv - Microbiology 2021
Quote:
... Extracts were run on a retinoic acid ELISA kit (MyBiosource, MBS706971) according to manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Daniel Neumeier, et al.,
bioRxiv - Immunology 2021
Quote:
... protein extracts from commercial bone marrow tissue (Zyagen, MT-704-C57) as well as self-made extracts from spleen and bone marrow were tested at a 100ug/ml coating concentration ...
-
No products found
because this supplier's products are not listed.
Ananya Acharya, et al.,
bioRxiv - Biochemistry 2023
Quote:
... the cell extracts were supplemented with 50 nM Okadaic acid (APExBIO), 1 mM sodium orthovanadate (Sigma) ...
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No products found
because this supplier's products are not listed.
Hasan Alhaddad, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Protein extracts were immunoblotted using antibodies against SERPINA3 (1:1000, Genetex), phospho-ERK (Thr202/Tyr204 ...
-
No products found
because this supplier's products are not listed.
Flora Paldi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Yeast were mounted onto a glass slide mounted in Vectashield (Vector Laboratories, Peterborough UK) and imaged on a Zeiss Axio Imager Z1 equipped with a x100 α Plan Fluar/1.45 NA (oil ...
-
No products found
because this supplier's products are not listed.
Nathalie Bastié, et al.,
bioRxiv - Genetics 2021
Quote:
... yeast cells were synchronised in G1 by adding of α-factor (Antibodies-online, ABIN399114) in the media every 30min during 2h30 (1μg/mL final) ...
-
No products found
because this supplier's products are not listed.
Fatemeh Mazloumi Gavgani, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and the light extracts were incubated with control beads (Echelon Biosciences P-B000) for 1 h rotating at 4°C ...
-
No products found
because this supplier's products are not listed.
Chiara Cassioli, et al.,
bioRxiv - Immunology 2020
Quote:
... Protein extracts were quantified with a BCS Total Protein Assay Kit (Euroclone, #EMP014500) and denatured in 4X Bolt LDS Sample Buffer (Life Technoogies ...
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No products found
because this supplier's products are not listed.
Yu-Te Yeh, et al.,
bioRxiv - Cell Biology 2022
Quote:
Insulin measurements from plasma and pancreatic extracts were measured by ELISA (Crystal Chem), blood glucose and luciferase assays were measured as described (Poy et al. ...
-
No products found
because this supplier's products are not listed.
Ying Liu, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Protein concentrations in the extracts were measured by BCA assay (AR1189A, BOSTER, Wuhan, CHN). Equal amounts of extracts were separated by SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Fritzsche Stefanie, et al.,
bioRxiv - Bioengineering 2021
Quote:
... redissolved extracts were filtered through syringe filters (0.22 μm, polyether sulfone, Sarstedt, Nümbrecht, Germany) in sterile 1.5-mL reaction vessels (Sarstedt ...
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No products found
because this supplier's products are not listed.
Devesh C. Pant, et al.,
bioRxiv - Neuroscience 2022
Quote:
Whole cell extracts were isolated using RIPA Lysis Buffer pH 7.4 (Bio-world, USA) supplemented with Halt™ protease and phosphatase inhibitor cocktail (ThermoFisher Scientific) ...
-
No products found
Yi Ran, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... cell extracts (3 ml of the supernatant) were incubated with Anti-Flag Affinity Gel (Bimake) at 4 °C for 3 h ...
-
No products found
because this supplier's products are not listed.
Isadora Matias, et al.,
bioRxiv - Neuroscience 2021
Quote:
Protein concentration in cell extracts was measured by the BCA Protein Assay Kit (Cole-Parmer). Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore ...
-
No products found
because this supplier's products are not listed.
Cécile Guth, et al.,
bioRxiv - Immunology 2023
Quote:
... Protein concentrations of crude extracts were determined using the Advanced Protein Assay (Cytoskeleton, Denver, USA).
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No products found
because this supplier's products are not listed.
Parbir S. Grewal, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
Oleate induction media was prepared as 6.7 g/L Difco Yeast Nitrogen Base without amino acids (Spectrum Chemical); 2 g/L Drop-out Mix Synthetic minus appropriate amino acids ...
-
No products found
because this supplier's products are not listed.
Benjamin J. Hanrahan, et al.,
bioRxiv - Genomics 2023
Quote:
... Tissue extracts were homogenized using T10 Basic ULTRA-TURRAX® Homogenizer (IKA, Staufen im Breisgau, Germany) and extracted using TRIzol reagent following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Zeyu Chen, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... a solution of 10 μg HDM extract (Stallergen Greer) and 500 ng SEB (List Biological Laboratories) in PBS was applied on a 1 cm2 gauze pad placed on the shaved skin and occluded with a Tegaderm Transparent Dressing (3 M HealthCare) ...
-
No products found
because this supplier's products are not listed.
Mitchell A. Ellison, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Yeast cells were grown to log phase in SC-L-W medium containing 1 mM BPA (Bachem F-2800). Crosslinking was performed as described (27) ...
-
No products found
because this supplier's products are not listed.
Meredith A. Skiba, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... and 187.5 μg nanobody insert DNA were transformed into 7.5E9 BJ5465 yeast with a 96-well ECM 830 Electroporator (BTX-Harvard Apparatus). Yeast were recovered in YPAD for 1.5 hours ...
-
No products found
because this supplier's products are not listed.
Hojjat Naderi-Meshkin, et al.,
bioRxiv - Cell Biology 2022
Quote:
... protein extracts of VOs were collected and analysed using the human protein array (RayBiotech, AAH-ANG-1000-8). To use conditioned media for protein array investigation ...
-
No products found
because this supplier's products are not listed.
Rebecca Cheung, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Precleared extract was incubated with Dynabeads Protein G beads conjugated with 6μg of mouse-anti-AGO2 (clone E12-1C9, Abnova) or mouse IgG (NXA931 ...
-
No products found
because this supplier's products are not listed.
Robin Mesnage, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... Cells were exposed to five concentrations of the test samples in the absence and presence of 0.25% S9 extract and required co-factors (RegenSysA+B, Moltox) for 24 h ...
-
No products found
because this supplier's products are not listed.
Angelo Cardoso Batista, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... the Avestin EmulsiFlex-C3 homogeniser was used at 15000-20000 psi and the final pellet-free cell extract was dialysed (using a Spectra/Por 4 Dialysis Tubing, 12-14 kD MWCO from Repligen) overnight in S30B buffer before one final centrifugation at 12000xg for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Vilasagaram Srinivas, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The genomic DNA was purified from the hippocampal region of the brain tissues (∼10-12mg) using the smart extract-DNA extraction kit as per the supplier’s instruction (Cat# SKDNEX-100, Eurogentec, Belgium). The quantity of total genomic DNAs (gDNA ...
-
No products found
because this supplier's products are not listed.
Thomas C. Williams, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... Crude DNA extracts were transferred to a 384-well plate compatible with the Echo 550 acoustic liquid transfer system (Labcyte), as were primer pairs for each PCR-tag in synXIV (File S2 ...