-
No products found
because this supplier's products are not listed.
M. Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
... Nile Red (Enzo Life Sciences) was added to the culture (2 μg/ml final concentration ...
-
No products found
because this supplier's products are not listed.
Rhiannon R. Penkert, et al.,
bioRxiv - Cell Biology 2021
Quote:
... GST lysates were incubated with glutathione agarose resin (GoldBio; 50 μL resin per 0.5-1.5 mL of lysate) for 30 min at 4°C and then washed 6x (3x quick washes ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Yang S. Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Lysates were sonicated by Covaris Sonicator to ~500 bp fragments (130 μL/tube ...
-
No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Erin R. Scheidemann, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... CTSD for mouse cell lysates (#MAB1123-SP) and CTSA for mouse cell lysates (AF1029-SP) were bought from R&D Systems.
-
No products found
because this supplier's products are not listed.
Samantha J Borland, et al.,
bioRxiv - Cell Biology 2020
Quote:
VSMC lysates were analyzed for PKCα (Proteintech 21991-1-AP), phosphorylated MARK2 ...
-
No products found
because this supplier's products are not listed.
Debashree Goswami, et al.,
bioRxiv - Cell Biology 2022
Quote:
... One mL of lysate was subsequently processed using the Abbott m2000sp using mSample RNA preparation kit (Abbott, Niles, IL). The eluate was tested by qRT-PCR for the pan-Plasmodium 18S rRNA target ...
-
No products found
because this supplier's products are not listed.
Heather J. Clemons, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... Each element was located and analyzed using SpotReader (Niles Scientific) or GenePix Pro 6.0 (Molecular Devices). The microarrays were submitted to the Stanford Microarray Database for further analysis [103] ...
-
No products found
because this supplier's products are not listed.
Doowon Huh, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Lysate was digested with RNase I (Lucigen) before ribosomes were isolated through ultracentrifugation through a sucrose cushion ...
-
No products found
because this supplier's products are not listed.
Anniek Zaalberg, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Lysates were sonicated (EpiShear Probe Sonicatore, Active Motif) for 10 cycles with one second intervals and a 20% amplitude using ...
-
No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... virus containing supernatants were clarified using a 0.45 µM PDVF filter (Pall Corp, NY, USA # 4614) and pelleted by ultracentrifugation on a 15% sucrose cushion before resuspension in culture media to achieve a 20X concentration over the original volume ...
-
No products found
because this supplier's products are not listed.
Rasha Khaddaj, Roger Schneiter,
bioRxiv - Cell Biology 2022
Quote:
... Images of Nile Red stained cells were collected using a Leica TCS SP5 (Leica, Wetzlar, Germany) confocal microscope equipped with a 63x/1.20 HCX PL APO objective ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Prashant Rajbhandari, et al.,
bioRxiv - Cell Biology 2019
Quote:
Whole cell lysate or tissue lysate was extracted using RIPA lysis buffer (Boston Bioproducts) supplemented with complete protease inhibitor cocktail (Roche) ...
-
No products found
because this supplier's products are not listed.
Robbert Boudewijns, et al.,
bioRxiv - Microbiology 2020
Quote:
... virus stocks were confirmed to be free of mycoplasma (PlasmoTest, InvivoGen) and other adventitious agents by deep sequencing on a MiSeq platform (Illumina ...
-
No products found
because this supplier's products are not listed.
Kuei-Chen Wang, et al.,
bioRxiv - Immunology 2023
Quote:
... or heart tissue lysate (Novus Biologicals) was resolved on 4-20% gradient SDS-PAGE (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Patrick Jouandin, et al.,
bioRxiv - Genetics 2019
Quote:
... Lysates were resolved by electrophoresis (Mini-PROTEAN TGX Precast Gels ...
-
No products found
because this supplier's products are not listed.
Jenny Y. Pham, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
This study used Escherichia virus T4 (American Type Culture Collection [ATCC] #11303-B4) and three wild-type bacterial hosts ...
-
No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
-
No products found
because this supplier's products are not listed.
Prasun Kundu, et al.,
bioRxiv - Microbiology 2022
Quote:
Purified biotinylated proteins were multimerised on streptavidin coated Nile Red fluorescent beads (Spherotech Inc ...
-
No products found
because this supplier's products are not listed.
Temitayo T. Bamgbose, et al.,
bioRxiv - Immunology 2023
Quote:
... and Nile red fluorescent intensity was quantified using cellSens Dimension 1.16 (OLYMPUS, Tokyo, Japan)
-
No products found
because this supplier's products are not listed.
C. N. Byrne-Hoffman, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... blocked with Mouse IgG (1:100, concentration, Jackson Labs, West Grove, PA), stained with FITC-IL12RB2 (R&D Systems) ...
-
No products found
because this supplier's products are not listed.
Heejoo Kim, et al.,
bioRxiv - Immunology 2020
Quote:
... Lysates were incubated with 2.5 μg Jmjd1A antibody (Bethyl) and protein-G Dynabeads (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Weichao Huang (黄伟超), et al.,
bioRxiv - Plant Biology 2023
Quote:
... and the Nile Red fluorescence emission quantified at 575 nm following excitation at 530 nm using a microplate reader (Infinite M1000; TECAN).
-
No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
-
No products found
Svenja Fritzlar, et al.,
bioRxiv - Microbiology 2023
Quote:
... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
Yoshiaki Nishimura, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were grown to ~90% confluency in a 96-well plate prior to addition of serially diluted virus to achieve luminescence signals of 30,000-1,000,000 (BMG Labtech PolarStar Optima plate reader at maximum gain) ...
-
No products found
because this supplier's products are not listed.
Yilin Wang, et al.,
bioRxiv - Immunology 2021
Quote:
... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
-
No products found
because this supplier's products are not listed.
Bei Zhao, Yinghua Liu, Xuemei Tang, Shi Cheng,
bioRxiv - Cancer Biology 2022
Quote:
... RIPA lysate (Solarbio, Beijing, China), N,N′-methylenebisacrylamide (Aladdin ...
-
No products found
because this supplier's products are not listed.
Michael Hulse, et al.,
bioRxiv - Microbiology 2020
Quote:
Nile Red fluorescence staining was assessed with the Lipid Droplets Fluorescence Assay Kit according to the manufacturer’s protocol (Cayman Chemical, Ann Arbor, MI, USA). One day before staining assay ...
-
No products found
because this supplier's products are not listed.
Hua Qin, et al.,
bioRxiv - Microbiology 2023
Quote:
... immunoreactive bands were detected with the ChemiGlow West Chemiluminescence Substrate Kit (Protein Simple). FLAG epitope-labeled lactate dehydrogenase (LDH ...
-
No products found
because this supplier's products are not listed.
Enakshi Roy, Wen Shi, Bin Duan, St Patrick Reid,
bioRxiv - Microbiology 2020
Quote:
... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
-
No products found
because this supplier's products are not listed.
Qiulong Yan, et al.,
bioRxiv - Microbiology 2020
Quote:
The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Jian Hang Lam, et al.,
bioRxiv - Immunology 2022
Quote:
... with Trans IT-Virus GEN (Mirus). In brief ...
-
No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Pengxiang Chang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in HBS-EP buffer (TEKnova) containing 10 μM oseltamivir carboxylate (Roche ...
-
No products found
because this supplier's products are not listed.
Philip Kawalec, et al.,
bioRxiv - Physiology 2022
Quote:
... Serum Troponin-I was measured using the Ultra-Sensitive Mouse Cardiac Troponin-I ELISA Kit purchased from Life Diagnostics (CTNI-1-US; Life Diagnostics; West Chester, PA).
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
-
No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
-
No products found
because this supplier's products are not listed.
Emily A. Hemann, et al.,
bioRxiv - Immunology 2022
Quote:
... this virus/serum mixture is incubated with 0.5% chicken red blood cells (Rockland) and hemagglutination inhibition was measured after 30 minutes.
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...
-
No products found
because this supplier's products are not listed.
Lauren Rodriguez, et al.,
bioRxiv - Microbiology 2019
Quote:
... The lysate was subjected to bead beating (Mini-Beadbeater, Biospec Products) followed by a chloroform extraction ...
-
No products found
because this supplier's products are not listed.
Michael Bauer, et al.,
bioRxiv - Microbiology 2020
Quote:
... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
-
No products found
because this supplier's products are not listed.
Teodors Pantelejevs, Marko Hyvönen,
bioRxiv - Biochemistry 2022
Quote:
... lysate was loaded on a 3 mL Ni-NTA agarose matrix (Cube Biotech), after which column matrix was washed with 10 CV Nickel Buffer A ...
-
No products found
because this supplier's products are not listed.
Xiaofeng Zheng, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Insulin and protein content of the lysate were measured using Mouse Insulin ELISA (Mercodia) and BCA assay (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
-
No products found
because this supplier's products are not listed.
Guus Franken, et al.,
bioRxiv - Immunology 2023
Quote:
... Serially diluted lysates were arrayed on nitrocellulose-coated slides (Grace Bio-Labs) by the Quanterix (Aushon ...