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No products found
because this supplier's products are not listed.
M. A. Rossotti, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Virus infection was detected with mouse mAb to SARS-CoV- 2 nucleocapsid (N) (R&D Systems, Minneapolis, MN, Cat#MAB10474) and counterstained with rabbit anti-mouse IgG-HRP (Rockland Immunochemicals ...
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No products found
because this supplier's products are not listed.
Debashree Goswami, et al.,
bioRxiv - Cell Biology 2022
Quote:
... One mL of lysate was subsequently processed using the Abbott m2000sp using mSample RNA preparation kit (Abbott, Niles, IL). The eluate was tested by qRT-PCR for the pan-Plasmodium 18S rRNA target ...
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No products found
because this supplier's products are not listed.
Stephanie Pei Tung Yiu, et al.,
bioRxiv - Microbiology 2024
Quote:
... Precleared lysate was then incubated with 2 μg/ml anti-GFP antibody (Proteintech #50430-2-AP) for 1 hr at 4°C with rotation ...
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No products found
because this supplier's products are not listed.
Stephen Graham, et al.,
bioRxiv - Immunology 2020
Quote:
... The virus-monoclonal antibody mixture was added (2 mL) to Vero cells in a 6-well plate (Greiner). After infecting the cells for 1 hour at 37°C ...
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No products found
because this supplier's products are not listed.
M. Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
... Nile Red (Enzo Life Sciences) was added to the culture (2 μg/ml final concentration ...
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No products found
because this supplier's products are not listed.
Paulo V. M. Boratto, et al.,
bioRxiv - Microbiology 2020
Quote:
... the μl of virus solution were placed on glow discharged C-Flat 2/2 grids (EMS) and plunge frozen into liquid ethane using a Gatan Cryoplunge 3 ...
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No products found
because this supplier's products are not listed.
Temitayo T. Bamgbose, et al.,
bioRxiv - Immunology 2023
Quote:
... and Nile red fluorescent intensity was quantified using cellSens Dimension 1.16 (OLYMPUS, Tokyo, Japan)
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No products found
because this supplier's products are not listed.
Rasha Khaddaj, Roger Schneiter,
bioRxiv - Cell Biology 2022
Quote:
... Images of Nile Red stained cells were collected using a Leica TCS SP5 (Leica, Wetzlar, Germany) confocal microscope equipped with a 63x/1.20 HCX PL APO objective ...
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Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...
Cat# LS004174,
100 mg, $42.00
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Brigitte E. Martin, et al.,
bioRxiv - Microbiology 2020
Quote:
... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
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No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Piyali Majumder, et al.,
bioRxiv - Neuroscience 2020
Quote:
... post-mortem whole brain lysates and Type 2 Diabetic (NB820-59232) and Non-Diabetic (NB820-59291) post-mortem whole liver lysates were purchased from Novus Biologicals. For statistical reasons we procured products from different patients with different lot numbers (for patients details see Table S1a and Table S1b).
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No products found
because this supplier's products are not listed.
Rhiannon R. Penkert, et al.,
bioRxiv - Cell Biology 2021
Quote:
... GST lysates were incubated with glutathione agarose resin (GoldBio; 50 μL resin per 0.5-1.5 mL of lysate) for 30 min at 4°C and then washed 6x (3x quick washes ...
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No products found
because this supplier's products are not listed.
Heather J. Clemons, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... Each element was located and analyzed using SpotReader (Niles Scientific) or GenePix Pro 6.0 (Molecular Devices). The microarrays were submitted to the Stanford Microarray Database for further analysis [103] ...
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No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Sophie I Holland, et al.,
bioRxiv - Microbiology 2020
Quote:
... was fitted with a Luna C18(2) column (150 × 4.6 mm, 5 µm; Phenomenex, Lane Cove West, Australia). The mobile phases were 0.5 mM ammonium acetate in water (A ...
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No products found
because this supplier's products are not listed.
Sinem Yılmaz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The nuclear lysates were subjected to Nrf-2 Transcription Factor Assay (Cayman Chemical, US, 600590) to evaluate the effect of CA on the nuclear Nrf-2 activity ...
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No products found
because this supplier's products are not listed.
Tanay Bhatt, et al.,
bioRxiv - Pathology 2022
Quote:
About 0.1 to 0.2 MOI (Multiplicity of Infection) equivalent of VSV-G and pseudotype SARS-CoV-2 spike virus particles were treated with recombinant LL37 (Tocris) and niacinamide (vitamin B3 ...
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No products found
because this supplier's products are not listed.
Mingming Chen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...
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No products found
because this supplier's products are not listed.
M. Claireaux, et al.,
bioRxiv - Immunology 2023
Quote:
Pseudovirus was produced by co-transfecting the pCR3 SARS-CoV-2–SΔ19 expression plasmid with the pHIV-1NL43 ΔEnv-NanoLuc reporter virus plasmid in HEK293T cells (American Type Culture Collection, CRL-11268) (61 ...
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No products found
because this supplier's products are not listed.
Yang S. Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Lysates were sonicated by Covaris Sonicator to ~500 bp fragments (130 μL/tube ...
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No products found
because this supplier's products are not listed.
Huan Chen, et al.,
bioRxiv - Microbiology 2021
Quote:
... the co-immunoprecipitation sample and 2% whole-cell lysates were analyzed by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Pall Corp.). The membrane was blocked in 3% skim milk in phosphate buffered saline with Tween 20 (PBST ...
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No products found
because this supplier's products are not listed.
Arthur Flohr Svendsen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Virus was produced by transfecting Platinum-E retroviral packaging cells (Cell Biolabs) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
C. N. Byrne-Hoffman, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... blocked with Mouse IgG (1:100, concentration, Jackson Labs, West Grove, PA), stained with FITC-IL12RB2 (R&D Systems) ...
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No products found
because this supplier's products are not listed.
Xiaowen Duan, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
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No products found
because this supplier's products are not listed.
Anniek Zaalberg, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Lysates were sonicated (EpiShear Probe Sonicatore, Active Motif) for 10 cycles with one second intervals and a 20% amplitude using ...
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No products found
because this supplier's products are not listed.
Sebastian Tacke, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plain grids (Quantifoil 2/2) were transferred to the microscope ...
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No products found
because this supplier's products are not listed.
Hsiang-i Tsai, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant human interleukin-2 (2 ng/ml, IL-2, Peprotech). Ficoll® lymphocyte separation medium (LSM ...
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No products found
because this supplier's products are not listed.
Eugenia Zah, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The lysate supernatant was ultracentrifuged with iodixanol (OptiPrep; StemCell Technologies) density gradient solutions (54% ...
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No products found
because this supplier's products are not listed.
Christopher M. Schafer, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Incubation of cell lysates with polyubiquitin-affinity beads (Calbiochem: #662200-1KIT) was carried out according to the manufacturer’s protocol followed by immunoblotting with an anti-ERG Ab (Santa Cruz ...
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No products found
because this supplier's products are not listed.
Sarayu Row, Wu-Min Deng,
bioRxiv - Developmental Biology 2020
Quote:
... the ovaries were incubated in Nile red (TCI America, 7385-67-3) (0.002% dye diluted in PBT ...
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No products found
because this supplier's products are not listed.
Peter Vandeberg, et al.,
bioRxiv - Immunology 2020
Quote:
Anti-SARS-CoV-2 IgG titers were determined using Human Anti-SARS-CoV-2 Virus Spike 1 (S1) IgG assay (Alpha Diagnostic). hIVIG batches were tested using multiple serial dilutions and a curve constructed by plotting the log of the optical density as a function of the log of the dilution ...
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No products found
because this supplier's products are not listed.
Michael Bauer, et al.,
bioRxiv - Microbiology 2020
Quote:
... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
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No products found
because this supplier's products are not listed.
Weichao Huang (黄伟超), et al.,
bioRxiv - Plant Biology 2023
Quote:
... and the Nile Red fluorescence emission quantified at 575 nm following excitation at 530 nm using a microplate reader (Infinite M1000; TECAN).
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No products found
because this supplier's products are not listed.
Prashant Rajbhandari, et al.,
bioRxiv - Cell Biology 2019
Quote:
Whole cell lysate or tissue lysate was extracted using RIPA lysis buffer (Boston Bioproducts) supplemented with complete protease inhibitor cocktail (Roche) ...
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No products found
because this supplier's products are not listed.
Bei Zhao, Yinghua Liu, Xuemei Tang, Shi Cheng,
bioRxiv - Cancer Biology 2022
Quote:
... RIPA lysate (Solarbio, Beijing, China), N,N′-methylenebisacrylamide (Aladdin ...
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No products found
because this supplier's products are not listed.
Christina Ross, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... A total of 200–500 μg of nuclear lysates were incubated with 2 μg of SMARCD1 antibody (Bethyl A301-594A) and 50 μg of Dynabeads Protein G (Invitrogen) ...
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No products found
because this supplier's products are not listed.
John W. Ribis, et al.,
bioRxiv - Microbiology 2023
Quote:
... Spores were analyzed on an Axioskop (Carl Zeiss, West-Germany) upright microscope ...
-
No products found
because this supplier's products are not listed.
Dongying Chen, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... To harvest protein lysate from IBIDI µSlide ...
-
No products found
because this supplier's products are not listed.
Patrick Jouandin, et al.,
bioRxiv - Genetics 2019
Quote:
... Lysates were resolved by electrophoresis (Mini-PROTEAN TGX Precast Gels ...
-
No products found
because this supplier's products are not listed.
Edward A. de Koning, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Light microscopy was performed using either 488 nm (imaging of GFP channel) or 561 nm (imaging of nile red channel) laser-based illumination through a 100x objective (Nikon CFI TU Plan Apo 100x/NA0.9). Intensities at the sample were kept below 50 W/cm2 to avoid sample heating and devitrification(63) ...
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No products found
because this supplier's products are not listed.
Xiao-Yu Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
... The brain lysate was 1:10 diluted in the PBS and stained with rhodamine-labeled dolichos biflorus agglutinin (DBA-red, Vector Laboratories, RL-1032-2). GFP and DBA-red double-positive cysts were counted and diluted to 5 cysts per 200 uL of PBS ...
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No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
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No products found
because this supplier's products are not listed.
David Ollitrault, et al.,
bioRxiv - Cell Biology 2021
Quote:
... cells were placed for 2 days in Endothelial Cell Growth Medium 2 (EGM-2; PromoCell) supplemented with 0.5 ng/ml of vascular endothelial growth factor 165 (VEGF) ...
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No products found
because this supplier's products are not listed.
Andrew M. Hogan, et al.,
bioRxiv - Microbiology 2019
Quote:
... The cells were washed and resuspended in PBS with 0.5 μg/mL Nile Red (Carbosynth) and stained at room temperature in the dark for 20 minutes ...
-
No products found
because this supplier's products are not listed.
Jian Hang Lam, et al.,
bioRxiv - Immunology 2022
Quote:
... with Trans IT-Virus GEN (Mirus). In brief ...
-
No products found
because this supplier's products are not listed.
K Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Lysates for all WTs within a cohort (Cohort 1: 2F + 3M; Cohort 2: 3M; Cohort 3: 4F; Cohort 4: 6M), and all KOs within a cohort were pooled (Cohort 1 ...
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No products found
because this supplier's products are not listed.
ER Forster, X Yang, HC Hang, A Shen,
bioRxiv - Microbiology 2022
Quote:
... Biotin was conjugated to the probes in a click reaction with 0.4 mg cleared lysate in 180 μL PBS and 20 μL 10X click master mix (1 mM azido-PEG3-biotin [Alfa Aesar], 10 mM tris(2-carboxyethyl)phosphine hydrochloride ...