-
No products found
because this supplier's products are not listed.
Jonathan Perr, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
-
No products found
because this supplier's products are not listed.
Fransenio Clark, et al.,
bioRxiv - Immunology 2022
Quote:
The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
-
No products found
because this supplier's products are not listed.
Min Zhang, et al.,
bioRxiv - Bioengineering 2020
Quote:
... the chip devices were sterilized in an autoclave and the porous member of the device was coated with rat tail collagen type I (200 μg/mL, Corning) on both sides at 37 °C for 48 h before cell seeding.
-
No products found
because this supplier's products are not listed.
Gang Wu, et al.,
bioRxiv - Immunology 2020
Quote:
... Tyramide-SS-biotin was from Iris Biotech GmbH ...
-
No products found
because this supplier's products are not listed.
Eri Morimoto, Kotaro Tsuboyama, Yukihide Tomari,
bioRxiv - Biochemistry 2022
Quote:
... Anti-mouse antibody was used as the secondary antibody at 1:100 (Protein Simple).
-
No products found
because this supplier's products are not listed.
Sean J. Ravel, Victoria M. Hollifield,
bioRxiv - Microbiology 2023
Quote:
... Controls samples included an 8-member positive controls (Zymo Research), as well as an extraction negative and a PCR negative controls ...
-
No products found
because this supplier's products are not listed.
Rajika Arora, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Probe specific for Tf L1 family was labeled with Red-dUTP (Enzo Life sciences) using a nick translation kit (Abbot) ...
-
No products found
because this supplier's products are not listed.
Maaike Allers, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Purified protein was shipped for antibody production (custom polyclonal antibodies, Eurogentec). Rabbit polyclonal anti-Shp1 was purified in house by affinity purification ...
-
No products found
because this supplier's products are not listed.
Brooke K. Hayes, et al.,
bioRxiv - Microbiology 2023
Quote:
... 3 μL of 3 mg/mL of protein was added to glow discharged UltrAuFoil holey gold grid (Quantifoil GmbH) using a Vitrobot Mark IV (Thermofisher ...
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No products found
because this supplier's products are not listed.
Gabriella Collu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The protein was concentrated to 3 mg/ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius). The purified protein was flash frozen in liquid nitrogen and stored at − 80 °C.
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No products found
because this supplier's products are not listed.
Katharina Kases, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... antibodies in Protein LoBind tubes (Eppendorf). Thereafter ...
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No products found
because this supplier's products are not listed.
Andreas Schoen, et al.,
bioRxiv - Immunology 2023
Quote:
... was coupled to 100 ng/IP Poly(I:C) (HMW) Biotin (Invivogen) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Li Wang, et al.,
bioRxiv - Systems Biology 2020
Quote:
... and the remainder was incubated with 3 ug anti-m6A polyclonal antibody (Synaptic Systems; 202003) which was preconjugated to Dynabeads Protein A (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Shengchen Lin, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Luciferase signal was read in FlexStation 3 (Molecular Devices).
-
No products found
because this supplier's products are not listed.
Ralph E Peterson, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
Three gerbil families (Meriones unguiculatus, n=6 per family: 2 adults, 4 pups) were used in this study (Charles River). All procedures related to the maintenance and use of animals were approved by the University Animal Welfare Committee at New York University ...
-
No products found
because this supplier's products are not listed.
Chaochao Dai, et al.,
bioRxiv - Immunology 2024
Quote:
... anti-TNFSF9 (tumor necrosis factor superfamily member 9) (#bs-3851R-APC) was from Bioss (Beijing, China); anti-interleukin (IL)-1β (#12-7018-41 ...
-
No products found
because this supplier's products are not listed.
David McClellan, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Biotin was obtained from TCI Chemicals and doxycycline (doxy ...
-
No products found
because this supplier's products are not listed.
Kit-Yi Leung, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... GLDC protein abundance was determined using anti-GLDC antibody (Atlas antibodies), with GAPDH (detected using anti-GAPDH ...
-
No products found
because this supplier's products are not listed.
Jonas Mårtensson, et al.,
bioRxiv - Immunology 2020
Quote:
... Sweden) and the Src family kinase inhibitor PP2 were from Calbiochem (La Jolla, CA, USA). PAF was obtained from Avanti Polar Lipids (Alabaster ...
-
No products found
because this supplier's products are not listed.
Aswini Panigrahi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Galectin-3-binding protein ORF was subcloned from its source vector (Origene # RC204918) into bicistronic lentiviral transfer vector pHR-CMV-TetO2 3C-TwinStrep-IRES-EmGFP vector (Addgene ...
-
No products found
because this supplier's products are not listed.
Penelope Lindsay, et al.,
bioRxiv - Plant Biology 2020
Quote:
... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
-
No products found
because this supplier's products are not listed.
Shao-Li Yang, et al.,
bioRxiv - Plant Biology 2021
Quote:
... the protein–chromatin solution was incubated with GFP antibody-conjugated magnetic beads (ChromoTek) under 4°C overnight ...
-
No products found
because this supplier's products are not listed.
Dipti Rai, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
-
No products found
because this supplier's products are not listed.
Andrew Dunbar, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific) and analyzed on a Cytation 3 plate reader (BioTek). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel and transferred to a nitrocellulose membrane ...
-
No products found
because this supplier's products are not listed.
Tanja Kuhm, et al.,
bioRxiv - Biophysics 2023
Quote:
... containing 3 moles of biotin per mole of BSA (BioVision). After removal of Biotin ...
-
No products found
because this supplier's products are not listed.
Krista K. Alexander, et al.,
bioRxiv - Biochemistry 2023
Quote:
... D-biotin was purchased from GoldBio. All solvents and other liquid reagents were purchased from Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Deike J. Omnus, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The final protein sample was concentrated (Pall Advance Centrifugal Device, MWCO 3 kDa), adjusted to 100 µM and stored in aliquots at −80°C ...
-
No products found
because this supplier's products are not listed.
Kangmin He, et al.,
bioRxiv - Cell Biology 2019
Quote:
... The antibody against GAK (M057-3) was purchased from MBL International Corp.
-
No products found
because this supplier's products are not listed.
Vanessa M. Doulames, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Rho-associated protein kinase inhibitor (3 uM; 1293823; PeproTech) was added to the media for 1 day post replating.
-
No products found
because this supplier's products are not listed.
Kirsten E. L. Garner, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The coverslip was then functionalized using PLL-PEG-Biotin (SuSOS), washed with 50 μl of TIRF buffer (30 mM HEPES pH 7.2 ...
-
No products found
because this supplier's products are not listed.
Ruoyu Jiang, et al.,
bioRxiv - Immunology 2023
Quote:
The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
-
No products found
because this supplier's products are not listed.
Destiny J. Davis, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cellulose was labeled with GFP-CBM3a (family 3 carbohydrate binding module; NZYtech, Lisboa, Portugal) following fixation with 1.5% PFA (PFA ...
-
No products found
because this supplier's products are not listed.
Lamiaa El-Shennawy, et al.,
bioRxiv - Immunology 2020
Quote:
... Biotin conjugated RBD protein (ACROBiosystems, SPD-C82E9) were coupled with magnetic beads (CELLection Biotin Binder Kit ...
-
No products found
because this supplier's products are not listed.
Hannah J. Loghry, et al.,
bioRxiv - Immunology 2022
Quote:
... Tim-3 was neutralized using InVivoMAb anti-mouse Tim-3 antibodies (10µg/mL) (Bio X Cell, Lebanon, NH) for 1 h prior to rBma-LEC-1 or rBma-LEC-2 treatment.
-
No products found
because this supplier's products are not listed.
Shuaijie Dou, et al.,
bioRxiv - Immunology 2021
Quote:
... and the antibody to Tim-3 (A2516) for western Blot was obtained from Abclonal.
-
No products found
because this supplier's products are not listed.
Wenbin Du, et al.,
bioRxiv - Plant Biology 2024
Quote:
Localization of ARC3-mNG and ARC6 (or its derivates)-mNG-3×HA fusion proteins in transgenic plants was detected using a laser scanning confocal microscope (Nikon A1 HD25) with a 100× oil immersion objective (numerical aperture ...
-
No products found
because this supplier's products are not listed.
Leïla Dumas, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 0.75 mM biotinylated UTP (Biotin-16-UTP, Lucigen BU6105H) and either 7.5 mM GTP or 6.75 mM 7-deazaguanine (TriLink N-1044 ...
-
No products found
because this supplier's products are not listed.
How-Wing Leung, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Lisa Houston, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... eEF3 protein was detected with anti-eEF3 antibody (Kerafast ED7003) and Glucose-6-phosphate dehydrogenase (G-6-PDH ...
-
No products found
because this supplier's products are not listed.
András Iring, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Detection was processed using biotin-free secondary antibodies (MACH 4 Universal HRP-Polymer, Biocare Medical LLC, Cat. No. M4U534). For visualization ...
-
No products found
because this supplier's products are not listed.
Indrajit Lahiri, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... we added 25-fold excess of biotin-labeling reagent (ChromaLink Biotin, Trilink, USA) to the sample and incubated the reaction for 2h at room temperature ...
-
No products found
because this supplier's products are not listed.
Linjia Su, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Pre-acetylated Biotin-Histone 4 Peptide was purchased from AnaSpec (#64989-025). Bovine serum albumin fraction V (#A7888) ...
-
No products found
because this supplier's products are not listed.
T S Sreevidya, et al.,
bioRxiv - Biophysics 2021
Quote:
The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
-
No products found
because this supplier's products are not listed.
Madhu Tiwari, et al.,
bioRxiv - Plant Biology 2021
Quote:
... The VirE2 protein was detected by the anti-VirE2 polyclonal antibody (G-Biosciences, 1:10000) and anti-rabbit secondary antibody (Sigma ...
-
No products found
because this supplier's products are not listed.
Daniela Di Girolamo, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 3.6 ug of Sens Target DNA are fragmented and biotin labelled using Encore Biotin Module kit (Tecan). After control of fragmentation using Bioanalyzer 2100 ...
-
No products found
because this supplier's products are not listed.
Wei-Siang Liau, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein identification was carried out using Protein Pilot 5.0 (SCIEX) for database searching.
-
No products found
because this supplier's products are not listed.
Yanqi Yu, et al.,
bioRxiv - Biophysics 2021
Quote:
... 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was purchased from Alfa Aesar (Haverhill, MA). Nigericin sodium salt was purchased from Tocris Bioscience (Minneapolis ...
-
No products found
because this supplier's products are not listed.
Qiuling Wang, Suiwen Hou,
bioRxiv - Plant Biology 2022
Quote:
... Protein concentration was determined using BCA protein assay kit (Solarbio, PC0021).
-
No products found
because this supplier's products are not listed.
Supratim Basu, et al.,
bioRxiv - Plant Biology 2021
Quote:
... The agro-infiltrated leaves were analyzed for protein localization at 3 dpi under a microscope (Olympus BX51-P) equipped with a UV light source ...
-
No products found
because this supplier's products are not listed.
Ryoma Hattori, Takaki Komiyama,
bioRxiv - Neuroscience 2021
Quote:
... The implanted GRIN lens was secured using 3 M Vetbond (WPI) on the skull ...