-
No products found
because this supplier's products are not listed.
Yusha Sun, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... containing 0.1 μL of 1 M Tris-HCl pH 8.3 (Hampton Research, HR2-900-14), 0.024 μL of 5 M NaCl (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Yu Takahashi, et al.,
bioRxiv - Genetics 2019
Quote:
... incubated in phloroglucinol-HCL solution for 1 min and observed with stereoscopic microscope SZX7 (OLYMPUS, Tokyo) for BC3F5 plants of azuki bean and yard-long bean ...
-
No products found
because this supplier's products are not listed.
Euido Nishijima, et al.,
bioRxiv - Neuroscience 2022
Quote:
Transient transfection in Cos-7 cells or Neuro2A cells was performed using Polyethylenimine HCl Max (Polyscience). After transfection for 20 h ...
-
No products found
because this supplier's products are not listed.
Ian W. McCahill, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Brightfield imaging of phloroglucinol-HCl and SBB sections was performed on an Eclipse E200 microscope (Nikon) and Pixelink camera (Pixelink) ...
-
No products found
because this supplier's products are not listed.
Andrew J. Lutkewitte, et al.,
bioRxiv - Physiology 2020
Quote:
... 100 mM Tris-HCl (pH 7.4)) containing 20 µM [14C]oleoyl-CoA (American Radiolabeled Chemicals, ARC 0527), and 200 µM sn-2-oleoylglycerol (Caymen Chemical ...
-
No products found
because this supplier's products are not listed.
Penghao Xu, et al.,
bioRxiv - Genomics 2023
Quote:
... neutralization using 2 M HCl and purification using HighPrep™ RNA Elite Clean-up System (MagBio Genomics) was performed ...
-
No products found
because this supplier's products are not listed.
Vivian Salgueiro, et al.,
bioRxiv - Microbiology 2023
Quote:
... samples were hydrolyzed overnight in 6N HCl at 100°C in 1 ml capacity-crystal ampoules (Wheaton). Samples were dried and resuspended in water and mixed in a 1:1:1 ratio with pure acetic acid and ninhydrin reagent (250 mg ninhydrin ...
-
No products found
because this supplier's products are not listed.
Y. M. Nuwan, D. Y. Bandara, Kevin J. Freedman,
bioRxiv - Biophysics 2022
Quote:
... The pH was adjusted by adding concentrated drops of HCl (SA48-1, Fischer Scientific) or KOH (LC192402, LabChem) and measured using Accumet AB200 pH meter.
-
No products found
because this supplier's products are not listed.
A. Reiser, et al.,
bioRxiv - Systems Biology 2019
Quote:
eGFP mRNA and eGFP mRNA with Cy5 label nucleotides (996 nucleotides, 1 mg/mL in 10 mM Tris-HCl and pH 7.5) were purchased from TriLink Biotechnologies ...
-
No products found
because this supplier's products are not listed.
A. Katherine Hatstat, et al.,
bioRxiv - Biochemistry 2020
Quote:
... cells were harvested and resuspended in TUBE lysis buffer (50mM Tris-HCl, pH 7.5, 0.15M NaCl, 1mM EDTA, 1% NP-40, 10% glycerol, published by LifeSensors, Inc.) with 1X protease inhibitor cocktail (Bimake ...
-
No products found
because this supplier's products are not listed.
Beatrice Dyring-Andersen, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 50 mM Tris-HCl and individually mixed using an ultra-Turrax blender (T 10 basic ultra, IKA, Staufen, Germany) for 30s x 8 while kept on ice ...
-
No products found
because this supplier's products are not listed.
Michael J. McFadden, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 50 μg of RNA were biotinylated in biotinylation buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA, 20 ng/μl MTSEA Biotin-XX (Biotium)) for 30 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Simone Caielli, et al.,
bioRxiv - Immunology 2023
Quote:
... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
-
No products found
because this supplier's products are not listed.
Coline Arone, et al.,
bioRxiv - Microbiology 2023
Quote:
... expressing C91-PL cells were diluted in TNE 1X (Tris/HCl pH7.4 NaCl, EDTA) and plated on glass-bottom fluorodishes (WPI) coated with 0.01% poly-L-lysine (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Sami T. Tuomivaara, et al.,
bioRxiv - Biochemistry 2023
Quote:
We mixed 97 μL of 100 mM Tris-HCl (pH 7.5) buffer with 2 μL of APEX2 and 1 μL of 100 mM Biotin-tyramide (BT, APExBIO) in DMSO onto a 96-well microplate ...
-
No products found
because this supplier's products are not listed.
Nathan Smith, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and 100 mM Tris-HCl pH 8.8) and pulverized by vortexing for >10 minutes with approximately 50 0.5mm stainless steel beads (Next Advance). The pulverized crystal seed stock was decanted and then centrifuged at 84×g for 1 min ...
-
No products found
because this supplier's products are not listed.
Nobuyuki Oguri, et al.,
bioRxiv - Pathology 2023
Quote:
... activity was measured at an enzyme concentration of 0.17 U/mL in 150 mM NaCl and 50 mM Tris-HCl (pH 7.4) with 300 μM Chromogenix S-2302 (Diapharma).
-
No products found
because this supplier's products are not listed.
Yahui Yan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The initial small crystals grew at 15% PEG 6000 and 0.1LM Tris-HCl pHL8.5 in ProPlex screen (Molecular Dimensions). After extensive optimisation of pH ...
-
No products found
because this supplier's products are not listed.
Avilash Singh Yadav, Adrienne H.K. Roeder,
bioRxiv - Developmental Biology 2024
Quote:
... and 1 ng/ml Thiamine HCl [40]) and 1000-fold diluted plant preservative mixture (PPM™, Plant Cell Technology).
-
No products found
because this supplier's products are not listed.
Hajime Futami, Mariko Sasaki, Takehiko Kobayashi,
bioRxiv - Molecular Biology 2024
Quote:
... DNA in the supernatant was precipitated with isopropanol and suspended in 250 μL of STE (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0], 100 mM NaCl containing 80 μg/mL RNaseA [Macherey-Nagel]). RNA was digested by incubation for 1 hr at 37°C ...
-
No products found
Sarah E. Arthur, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 40 mM Tris-HCl pH 8.0 at 25 °C) and 1.5 U/μL of T7 RNA polymerase (Applied Biological Materials). Reaction mixtures were incubated at 37°C for 2 h ...
-
No products found
because this supplier's products are not listed.
Xinrui L. Zhang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Cells were washed once with ice cold PBS and lysed in 130μL Sonication Buffer (10mM Tris-HCl pH 8, 0.25% SDS, 2mM EDTA, 1x protease inhibitor cocktail (Active Motif # 37490), 1mM PMSF) ...
-
No products found
Jonathan D. Lautz, et al.,
bioRxiv - Neuroscience 2021
Quote:
... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
-
No products found
because this supplier's products are not listed.
Martin P. Reichhardt, et al.,
bioRxiv - Immunology 2019
Quote:
... The flow-through was then acidified by addition of 1 μL 10 M HCl and injected onto a Dynamax 300-A□ C8 column (Rainin). The sample was eluted with a 0–80% ACN gradient in 0.1% TFA over 40 min ...
-
No products found
because this supplier's products are not listed.
Brian D. Rutter, et al.,
bioRxiv - Cell Biology 2022
Quote:
... vesicles resuspended in 20 mM Tris-HCl pH 7.5 were applied to glow-discharged 3.05-mm copper Formvar-carbon-coated electron microscopy grids (Electron Microscopy Sciences). After 1 minute ...
-
No products found
because this supplier's products are not listed.
Veronika Altmannova, et al.,
bioRxiv - Biochemistry 2022
Quote:
... were done in the commercial MST buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2; Nanotemper Technologies) supplied with 0.05% Tween-20 ...
-
No products found
because this supplier's products are not listed.
Stefano Donati, et al.,
bioRxiv - Systems Biology 2022
Quote:
... hydrolysis was performed by resuspending the cell pellets in 200μL of 6M HCl and transferring to 300μL - size FluidX tubes (Brooks Life Sciences). The tubes were sealed with FluidX rubber sealing mats (Brooks Life Sciences ...
-
No products found
because this supplier's products are not listed.
Samantha Mar, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... The spheroids were transferred into 500 μL acid ethanol (1 M HCl in 70% ethanol) for either human C-peptide ELISA (Alpco) or human glucagon ELISA (Mercodia).
-
No products found
Xiangyu Gong, et al.,
bioRxiv - Biophysics 2023
Quote:
... It was made sure about 4-5 spheroids were incorporated in 60uL gel solution per well in dopamine-HCl functionalized glass bottom 12-well plates (Cellvis). The gel droplets formed domes and were allowed to solidify for 1 hour at 37°C ...
-
No products found
because this supplier's products are not listed.
Linda Jansson, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Sample extracts were added to the Amicon tubes together with low TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0, Medicago, Uppsala, Sweden) to a total volume of 2 mL ...
-
No products found
because this supplier's products are not listed.
Philippe C Després, et al.,
bioRxiv - Evolutionary Biology 2024
Quote:
... the proteins were eluted in 1x BXT buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 1mM EDTA; IBA LifeSciences). We then exchanged the buffer to the protein buffer containing 20 mM Tris-HCl pH 8.0 ...
-
No products found
because this supplier's products are not listed.
Gareth T. Banks, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The columns were then washed with TBST (25 mM Tris-HCl [pH7.4], 138 mM NaCl, 0.05% Tween 20, National Diagnostics: EC-882) and conditioning buffer to remove non-bound components ...
-
No products found
because this supplier's products are not listed.
Bruno Motta Nascimento, Nikhil Unni Nair,
bioRxiv - Bioengineering 2020
Quote:
... coli were hydrolyzed 6 M HCl at 100 °C for 4 h in a vacuum sealed tube (Chemglass, #CG-4025-01). Acid was immediately removed with a vacuum centrifuge and pellet was resuspended in deionized water ...
-
No products found
because this supplier's products are not listed.
James T Nguyen, et al.,
bioRxiv - Physiology 2020
Quote:
... Mouse tails were snipped and digested in 100:1 ratio of Direct PCR Lysis Reagent (Tail) (FisherScientific #NC9724951) and Proteinase K (20mg/mL, 10mM Tris-HCl, pH 8.0, 1mM CaCl2, and 30% Glycerol) (IBI Scientific # IB05402) overnight 55°C ...
-
No products found
because this supplier's products are not listed.
Haruka Oda, et al.,
bioRxiv - Cell Biology 2022
Quote:
... were suspended in 20 mL ice-cold 50 mM Tris–HCl (pH 7.4) buffer containing 1 mg/mL lysozyme (Seikagaku Corporation, 100940) and 1× protease inhibitor cocktail (Nacalai Tesque ...
-
No products found
because this supplier's products are not listed.
Eliza C.B. Jaeger, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Sections were then incubated in DAPI in Tris-HCl for 30 min and mounted in Fluoromount-G® Mounting Medium (SouthernBiotech). Images were acquired using a confocal microscope (Zeiss LSM800 ...
-
No products found
because this supplier's products are not listed.
Simonas Juzenas, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... The reaction was terminated by quenching with a 10 mM Tris-HCl [pH 8.0] buffer supplemented with 50 µM methyltetrazine-DBCO (Click Chemistry Tools). The methanol-preserved cells conjugated with ClickTags were rehydrated following Supplementary Protocol 3.
-
No products found
because this supplier's products are not listed.
Tomohide Saio, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The reaction was quenched with an equivalent volume of 1 M HCl at the indicated time points and separated by RP-HPLC on a TSKgel Protein C4-300 column (Tosoh Bioscience, Japan) with monitoring at 229 nm ...
-
No products found
because this supplier's products are not listed.
Julia Kamenz, et al.,
bioRxiv - Cell Biology 2020
Quote:
... or in the case of the phosphorylated species of Arpp19 and ENSA on a 10% Tris-HCl SDS-PAGE supplemented with 10 μM Phos-tag reagent (NARD Institute, AAL-107) and then transferred onto PVDF membranes ...
-
No products found
because this supplier's products are not listed.
Kevin R. Coffey, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Tissue was stained in a 5 % potassium ferrocyanide and 10 % HCl and counterstained for antibodies against calbindin D28k immunohistochemistry (ImmunoStar, Inc., Hudson WI), as described previously(Coffey et al. ...
-
No products found
because this supplier's products are not listed.
Nivedya Swarnalekha, et al.,
bioRxiv - Immunology 2020
Quote:
... circumscribed with ImmEdge pen (Vector) and blocked in TBST (100mL Tris-HCl, 150mM NaCl, 0.05% Tween-20) containing 5% Donkey serum (Jackson ImmunoResearch #017-000-121) for 2 hours at RT ...
-
No products found
Mina Vaez, et al.,
bioRxiv - Bioengineering 2022
Quote:
Mono-dispersed collagen fibrils were produced by mixing 4 μl of type I tropocollagen monomers (3mg/mL in 0.01 N HCl, pH 2) from human placenta in acidic solutions (Advanced BioMatrix Inc., Carlsbad, CA) with a solution composed of 6 μl of UHQ water ...
-
No products found
because this supplier's products are not listed.
Ryan A. Martin, et al.,
bioRxiv - Physiology 2022
Quote:
... muscle samples were bead homogenized by a Qiagen Tissuelyser in reducing sample buffer (Tris-HCl, pH 6.8; 2% SDS; 10% glycerol; 1% β-mercaptoethanol) containing protease inhibitors (Cell Biolabs, AKR-190). Samples were centrifuged at 10,000 rpm at 4 °C ...
-
No products found
because this supplier's products are not listed.
Arumoy Chatterjee, et al.,
bioRxiv - Animal Behavior and Cognition 2021
Quote:
... 2-(3,4-dihydroxyphenyl)ethyl-1,1,2,2-d4-amine-HCl (dopamine-d4) and tryptamine-α,α,β,β-d4 were obtained from CDN Isotopes (Quebec, Canada). The deuterated internal standards 2-(4-Hydroxyphenyl)ethyl-1,1,2,2-d4-amine HCl and beta-Hydroxytyramine (α-d2 ...
-
No products found
because this supplier's products are not listed.
Takato Hiramatsu, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and 0.08 mM I2 in 25 mM HCl was taken in a glass capillary with its inner diameter of 1.13 mm (Drummond Scientific Company, Broomall, PA, USA) and placed on a sample stage ...
-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... bacteria cells were spin down and suspended in buffer H (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% Glycerol) containing 1 mg/ml lysozyme (Bio Basic, Cat. No. LDB0308-5) and 0.1 % TritonX-100 ...
-
No products found
because this supplier's products are not listed.
Isaac Framst, et al.,
bioRxiv - Microbiology 2022
Quote:
... Final elution was standardized across all DNA extraction methods with a volume of 100 μl in non-EDTA 10 mM tris-HCL elution buffer EDTA is known to interfere with downstream sequencing (Omega Bio-Tek, Norcross USA) (pH 8.5 and at 70 °C ...
-
No products found
because this supplier's products are not listed.
Gudrun Liebscher, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Snap- frozen samples of iBAT were homogenized in NP40-free lysis buffer (0.5 mM DTT in 50 mM Tris-HCl (pH 8.0) and freshly added protease and phosphatase inhibitors) using a tapered tissue grinder (DWK Life Sciences, Milville, New Jersey). The homogenate was passed through a 25G needle 6 times and centrifuged for 10 min at 1,000 x g and 4°C (pellet ...
-
No products found
because this supplier's products are not listed.
Lucie Grecias, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... We also exposed individuals to the SSRI fluoxetine for three days at a dose of 1mg / L (Fluoxetine HCl, BML-NS140, Enzo Life Sciences Inc., USA), known to result in behavioural changes similar to those induced by the presence of S ...
-
No products found
because this supplier's products are not listed.
Matthew Gabrielle, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Each cell pellet was resuspended in 500 µL of resuspension buffer (0.05 M HCl, 49% Methanol, 1% SPLASH Lipidomic Mass Spec Standard [Avanti Polar Lipids, Cat. Nu. 330707]), and 1 mL methyl-tert-butyl-ether was added ...