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No products found
because this supplier's products are not listed.
Tran N. H. Nguyen, et al.,
bioRxiv - Bioengineering 2024
Quote:
... We injected the culture medium through tubing (Tygon®, Cole-Parmer, IL, USA) connected to the injector loop at a 2.5 mL/min flow rate using a Fusion 200 syringe pump (Chemyx ...
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No products found
because this supplier's products are not listed.
Amaia Jauregi-Miguel, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... the 20 mL culture media was supplemented with 0.1µM biotin (LS-3500, Iris Biotech), and either 0.01 µM CHIR99021 (Cat ...
-
No products found
because this supplier's products are not listed.
Adham Safieddine, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cells were maintained in anti-bleaching live cell visualization medium (DMEMgfp; Evrogen), supplemented with 10% fetal bovine serum at 37°C in 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Saria Mcheik, et al.,
bioRxiv - Immunology 2019
Quote:
... Absolute cell numbers were determined by incorporating beads in the cell suspension (15 µm, Bangs Laboratories) and acquiring 15,000 beads ...
-
No products found
because this supplier's products are not listed.
Kei Haga, et al.,
bioRxiv - Microbiology 2021
Quote:
... cells were incubated with anti-NSP8 (5A10, GeneTex) or anti-dsRNA (rJ2 ...
-
No products found
because this supplier's products are not listed.
Maria Maloverjan, et al.,
bioRxiv - Cell Biology 2021
Quote:
... cells were incubated in cell culture medium containing 10 μM Draq5 DR50200 reagent (BioStatus, UK) for 3 min at 37 °C ...
-
No products found
because this supplier's products are not listed.
Alex S Genshaft, et al.,
bioRxiv - Systems Biology 2020
Quote:
... and conditioned media supplement Afamin-Wnt3a (10X, MBL international) was added to each well ...
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No products found
because this supplier's products are not listed.
Anna Cutrina-Pons, et al.,
bioRxiv - Immunology 2022
Quote:
... cell cultures were also treated with different concentration of IC87114 (LKT Laboratories Inc), a small molecule inhibitor of PI3K p110δ signalling used in the mouse studies ...
-
No products found
because this supplier's products are not listed.
K.G. Daniels, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... T cells were cryopreserved in RPMI1640 (UCSF cell culture core) with 20% human AB serum (Valley Biomedical, #HP1022HI) and 10% DMSO ...
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No products found
because this supplier's products are not listed.
Suheda Erener, et al.,
bioRxiv - Physiology 2019
Quote:
... islets and cell culture media using a Mouse Ultrasensitive Insulin ELISA (ALPCO, Salem, NH). Proinsulin was measured from plasma collected from cardiac blood using Rat/Mouse Proinsulin ELISA (Mercodia ...
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No products found
because this supplier's products are not listed.
Yedan Liu, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... of HBMVPs were seeded in a cell culture plate pre-coated with Cell-Tak Cell and Tissue Adhesive and incubated in pericyte growth medium (cAP-09, Angio-proteomie) for the following experiments ...
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No products found
because this supplier's products are not listed.
Cody J. Steely, et al.,
bioRxiv - Genomics 2021
Quote:
Patient ascites samples were grown in Human Breast Epithelial Cell Culture Complete Media (Celprogen M36056-01S); MCF7 whole cell lysate was purchased (Novus) ...
-
No products found
because this supplier's products are not listed.
Karen L. Kanke, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and plated in T75 culture flask at 5000 cells/ cm2 with iMatrix-511 (Nacalai USA #892021).
-
No products found
because this supplier's products are not listed.
Ana Kucera, et al.,
bioRxiv - Immunology 2020
Quote:
... T cell cultures were re-stimulated with DCs and 10 days later T cells were stained with MART-1 dextramer (Immudex, Copenhagen, Denmark) to assess the presence of MART-1 antigen-specific T cells in the cultures.
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No products found
because this supplier's products are not listed.
Livio Dukaj, Nicholas Rhind,
bioRxiv - Cell Biology 2020
Quote:
... cultures were vacuum-filtered and cells were resuspended in fresh YP-Raf supplemented with α-factor (Biomatik) to a final concentration of 25 nM ...
-
No products found
because this supplier's products are not listed.
Merve Mutlu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... cell culture chambers and transfected with the sgRNA library plasmid mix together with lentiviral packaging mix (Cellecta, containing psPAX2 and pMD2 plasmids that encode Gag/Pol and VSV-G ...
-
No products found
because this supplier's products are not listed.
Jakob Kreye, et al.,
bioRxiv - Immunology 2020
Quote:
... was expressed in HEK293T cells and immobilized onto 96-well plates from cell culture supernatant via anti-rabbit IgG (Dianova, 111-035-045) antibodies ...
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No products found
because this supplier's products are not listed.
Chenggong Ji, et al.,
bioRxiv - Biochemistry 2022
Quote:
... was incubated with serially diluted plasmodium proteins in 50 μl RPMI-1640 for 20 min and then mixed with equal volumes of OCI-Ly10 culture (~20,000 cells) and normal human serum complement (1:12.5 dilution, Quidel). The resulting mixtures were then transferred into a 96-microwell plate ...
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No products found
because this supplier's products are not listed.
Alessandro Bonifazi, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Cells were grown to confluence in α-MEM culture medium containing 10% FBS (Atlas Biologicals, Fort Collins, CO), 0.05% penicillin/streptomycin ...
-
No products found
because this supplier's products are not listed.
C. Inizan, et al.,
bioRxiv - Microbiology 2021
Quote:
... Viral antigens were detected in cell culture supernatants or detachment supernatants by Gag p24 ELISA according to the manufacturer’s instructions (Zeptometrix). When indicated ...
-
No products found
because this supplier's products are not listed.
Kjetil Hodne, et al.,
bioRxiv - Physiology 2019
Quote:
... In each culture 2-7 primary target cells were stimulated by 1 μM Gnrh1 (Bachem Americas, Inc. CA, USA).
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No products found
because this supplier's products are not listed.
Alyssa D. Cordero, et al.,
bioRxiv - Cell Biology 2020
Quote:
Polyclonal ASC culture ASC080414A (Zen-Bio, Inc.) was trypsinized and neutralized in DMEM supplemented with 20% FBS ...
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No products found
because this supplier's products are not listed.
Malika Gantier, et al.,
bioRxiv - Cell Biology 2022
Quote:
The quantity of human AFP and HGF secreted into the cell culture medium were determined using the AFP Human ELISA Kit (Abnova) and the HGF Human ELISA Kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Courtney A. Copeland, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Hypoxia-treated cells were transferred to a tissue culture glovebox set at 0.5% oxygen and 5% CO2 (Coy Lab Products). Medium was supplemented with DMSO 0.1% or BAY (10 μM ...
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No products found
because this supplier's products are not listed.
Matheus P. Viana, et al.,
bioRxiv - Cell Biology 2021
Quote:
... All cell lines were expanded and grown on an automated cell culture platform developed on a Hamilton Microlab STAR Liquid Handling System (Hamilton Company). This platform is summarized in part in Figure 1A ...
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No products found
because this supplier's products are not listed.
Leslie Duplaquet, et al.,
bioRxiv - Cancer Biology 2024
Quote:
The following chemicals (stored at −20°C or −80°C) were added to cell culture where indicated: BVdU was purchased from Chem-Impex International Inc. ...
-
No products found
because this supplier's products are not listed.
Tise Suzuki, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Total RNA from cell culture samples was isolated using IBI Isolate DNA/RNA Reagent Kit (#IB47602, IBI Scientific, Dubuque, IA, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Stephanie Torrino, et al.,
bioRxiv - Cell Biology 2020
Quote:
... collagen-coated hydrogel pre-plated in culture wells (Matrigen) was generated from a mix of acrylamide and bis-acrylamide coated with collagen ...
-
No products found
because this supplier's products are not listed.
Daniela Ramirez-Sanchez, et al.,
bioRxiv - Microbiology 2022
Quote:
... 6.0 g of plant tissue culture agar (PhytoTechnology Laboratories, A296), 1L of milli-Q water and a pH adjusted to range of 5.7-5.8 ...
-
No products found
because this supplier's products are not listed.
Weitong Yao, et al.,
bioRxiv - Microbiology 2021
Quote:
... 20 µL of cell culture supernatant of each sample and 100 µL of assay buffer containing 4 µM coelenterazine native (Biosynth Carbosynth, Cat. No. C-7001) were added to one well of a 96-well black opaque assay plate (Corning ...
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No products found
because this supplier's products are not listed.
Shou-Long Deng, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... and after 9 days of culture for acrosin (Bioss, Beijing, China, bs-5151R), a marker for spermatozoa [30] ...
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No products found
because this supplier's products are not listed.
George D. Moschonas, et al.,
bioRxiv - Microbiology 2023
Quote:
... culture medium was supplemented with recombinant human interferon-alpha 2 alpha (rhIFN-a2a) (#11343504, ImmunoTools), doxycycline (#D9891 ...
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No products found
Benoit Dehapiot, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... the culture medium was supplemented with the PLK1 inhibitor Bi-2536 (250 nM; Axon Medchem #1129) or the Rock-1 inhibitor Y-27632 (50 μM ...
-
No products found
because this supplier's products are not listed.
Jeremy Mom, et al.,
bioRxiv - Microbiology 2023
Quote:
... Genomic DNA was prepared from liquid cultures using the XIT Genomic DNA from Gram-Positive Bacteria kit (G-Biosciences), as instructed by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Soon-Gook Hong, et al.,
bioRxiv - Cell Biology 2023
Quote:
... cell monolayers were washed with 1X HBSS then imaged in culture media or 1X HBSS using Zeiss Colibri LED plus CMOS camera (Photometrics 95B) detection or LSM900 with Airyscan2 GaAsP-PMT detector for gentle confocal imaging.
-
No products found
because this supplier's products are not listed.
Minyue Qiu, et al.,
bioRxiv - Immunology 2022
Quote:
Myeloperoxidase (MPO) concentrations in Tregs and neutrophil culture supernatants were analyzed using an MPO (mouse) ELISA kit (BOSTER Biological Technology, Wuhan, China) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Dániel Molnár, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... The cells were disrupted using a cell disruptor (Scientific Industries SI-DD38 Digital Disruptor Genie Cell Disruptor ...
-
No products found
because this supplier's products are not listed.
Andrés López-Perrote, et al.,
bioRxiv - Biophysics 2020
Quote:
... coli cells (NZYTech) grown in LB medium ...
-
No products found
because this supplier's products are not listed.
Kashish Singh, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... coli cells (Genlantis) were used and the expression was induced overnight at 18°C with 0.1-0.25 mM IPTG added at O.D of 0.6-0.8 ...
-
No products found
because this supplier's products are not listed.
Ralf Schmidt, et al.,
bioRxiv - Immunology 2021
Quote:
... bulk T cells were frozen in Bambanker Cell Freezing Medium at 50e6 cells/ml (Bulldog Bio cat BB01) and stored at −80°C for short term or liquid nitrogen for long term storage ...
-
No products found
because this supplier's products are not listed.
Federico Tessadori, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Cell counting: cell counting was carried out in Volocity (Quorum technologies) on confocal-acquired 3D stacks.All statistical assays were carried out in Graphpad Prism 6.0 (GraphPad Software).
-
The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell...
Cat# B34304, SKU# B34304-25 mL (2500 rxns),
25 mL (2500 rxns), $201.00
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Wei Liu, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... to native K562 cells and the model cells were detected by cell counting kit-8 (CCK-8) (Bimake, Houston, USA). Cells were planted into 96-well plates in a density of 5000 cells/100uL medium/well ...
-
No products found
because this supplier's products are not listed.
Trieu Phan Hai, et al.,
bioRxiv - Microbiology 2020
Quote:
Protein digestion was confirmed by running treated and untreated culture filtrate on precast polyacrylamide gel 4-20 % (Mini-PROTEAN® TGX Stain-Free™ Precast Gels, BIO-RAD) at 200V for 40 mins ...
-
No products found
because this supplier's products are not listed.
Siv Anita Hegre, et al.,
bioRxiv - Molecular Biology 2021
Quote:
We performed cell counting using Moxi z mini automated cell counter (ORFLO Technologies) to investigate how knockdown of the lncRNA candidates affected cell growth ...
-
No products found
because this supplier's products are not listed.
Lawrence G. Welch, et al.,
bioRxiv - Cell Biology 2024
Quote:
... CD19+ B-cells (HemaCare, US) were cultured in B cell Excellerate medium with the CEllXVivo B cell expansion kit (R&D systems ...
-
No products found
because this supplier's products are not listed.
Nelson E. Bruno, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... The in cell western (LI-COR) was used to quantitate uptake of puromycin ...
-
No products found
because this supplier's products are not listed.
Niyati Jhaveri, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The flow cell (Akoya Biosciences, #240204) was assembled onto the slide as described in the user guide ...
-
No products found
because this supplier's products are not listed.
Rory Hennell James, et al.,
bioRxiv - Biochemistry 2022
Quote:
... protein complexes were overexpressed in BL21(DE3) cells and then extracted from cell membranes using lauryl maltose neopentyl glycol (LMNG; Anatrace). Protein complexes were then affinity-purified using StrepTactin XT resin (IBA ...
-
No products found
because this supplier's products are not listed.
Jeffrey S. Bourgeois, et al.,
bioRxiv - Microbiology 2020
Quote:
... cells were stained with 7-aminoactinomycin D (Biomol) and analyzed on a Guava Easycyte Plus flow Cytometer (Millipore) ...
-
No products found
because this supplier's products are not listed.
Dan-Hua Li, et al.,
bioRxiv - Microbiology 2021
Quote:
Cell morphology was determined by transmission electron microscopy (JEM-1400; JEOL). The utilization of carbon sources was determined using the 96 well BIOLOG AN microplate (BIOLOG Inc. ...