-
No products found
because this supplier's products are not listed.
Wei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... Parasites were stained with α-Toxoplasma gondii antibody (Abcam; see Supplementary table 6) without permeabilisation ...
-
No products found
because this supplier's products are not listed.
Nicolas Dos Santos Pacheco, et al.,
bioRxiv - Microbiology 2022
Quote:
Toxoplasma gondii tachyzoites were amplified in human foreskin fibroblasts (HFFs, ATCC) in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 5% of Fetal Bovine Serum (FCS ...
-
No products found
because this supplier's products are not listed.
Simon Butterworth, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... gondii (Santa Cruz) for 1 h at room temperature followed by 1:10,000 goat anti-rat IRDye 800CW (LI-COR ...
-
No products found
because this supplier's products are not listed.
Zisis Koutsogiannis, et al.,
bioRxiv - Microbiology 2022
Quote:
... RNA was isolated from freshly purified tachyzoites of Toxoplasma gondii using RNeasy Micro Kit (Qiagen) subsequently reverse-transcribed into first-strand cDNA using Qiagen one step RT-PCR kit System.
-
No products found
because this supplier's products are not listed.
Nurul Fadilah Ali Polanunu, et al.,
bioRxiv - Microbiology 2021
Quote:
... Antibody IgM and IgG levels for Toxoplasma gondii were measured by using kits from MyBioSource, Sandiego-California-USA ...
-
No products found
because this supplier's products are not listed.
Lorenzo Brusini, et al.,
bioRxiv - Microbiology 2021
Quote:
Toxoplasma gondii tachyzoites were grown in human foreskin fibroblasts (HFFs, American Type Culture Collection-CRL 1634) maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Simon Butterworth, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... gondii RHΔHXGPRT were transfected with KpnI-HF-digested (NEB) perturb-seq vectors by electroporation using the Amaxa 4D Nucleofector system (Lonza ...
-
No products found
because this supplier's products are not listed.
Karla M. Márquez-Nogueras, et al.,
bioRxiv - Microbiology 2020
Quote:
... gondii RHTatiΔKu80 parasites using a Gene Pulser X Cell electroporator (BioRad). Selection was done with 20 μM chloramphenicol ...
-
No products found
because this supplier's products are not listed.
Arne L. ten Hoeve, et al.,
bioRxiv - Microbiology 2024
Quote:
... gondii tachyzoites of the indicated strains and lysed in TRI Reagent (Sigma-Aldrich) or Lysis buffer (Jena Bioscience) ...
-
No products found
because this supplier's products are not listed.
Amol K. Bhandage, et al.,
bioRxiv - Immunology 2021
Quote:
... gondii (RH-LDM) were sorted (FACS Aria Fusion, BD Biosciences) based on GFP-expression and CD56 expression ...
-
No products found
because this supplier's products are not listed.
Francine Bittencourt Schiffler, et al.,
bioRxiv - Microbiology 2023
Quote:
... gondii (Dako, Carpinteria®, California, USA) using standardized protocols [46] ...
-
No products found
because this supplier's products are not listed.
Pragati Mastud, Swati Patankar,
bioRxiv - Cell Biology 2019
Quote:
... gondii RH strain using TRIsoln (Merck Genei). The cDNA for TgTPx1/2 was generated using gene specific reverse primers TPx1/2R by RevertAid Reverse Transcriptase (Thermo Scientific) ...
-
No products found
because this supplier's products are not listed.
Yifan Wang, et al.,
bioRxiv - Microbiology 2019
Quote:
... The GRA7 blot was incubated with a secondary antibody (HRP)-conjugated anti-rabbit antibody (Roche). The HRP was detected using an enhanced chemiluminescence (ECL ...
-
No products found
because this supplier's products are not listed.
June Guha, et al.,
bioRxiv - Immunology 2022
Quote:
... or the Toxoplasma specific peptides AS15 and ROP5 (custom made, Genscript) for 4 hours ...
-
No products found
because this supplier's products are not listed.
Franziska Hildebrandt, et al.,
bioRxiv - Microbiology 2023
Quote:
... gondii infected BMDCs were deposited into 384-well plates (Eppendorf twin.tecTM PCR plates) containing 2.3 μl of lysis buffer 114 using a CyClone™ robotic arm and at highly stringent single cell sort settings (single mode ...
-
No products found
because this supplier's products are not listed.
Jacqueline M Leung, et al.,
bioRxiv - Cell Biology 2019
Quote:
... gondii gDNA was prepared from freshly egressed parasites and extracted using the Wizard Genomic DNA Purification kit (Cat# A1120, Promega, Madison, WI).
-
No products found
because this supplier's products are not listed.
Akihiro Fujikawa, et al.,
bioRxiv - Biochemistry 2019
Quote:
Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
-
No products found
because this supplier's products are not listed.
Sandeep Srivastava, et al.,
bioRxiv - Microbiology 2020
Quote:
... Toxoplasma α-Centrin-1 (Kerafast company) 1:2,000 ...
-
No products found
because this supplier's products are not listed.
Wei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
Freshly lysed Toxoplasma tachyzoites were transfected with Amaxa 4D-Nucleofector system (Lonza). ~1×106 parasites were centrifuged and resuspended in 50 μl P3 buffer ...
-
No products found
because this supplier's products are not listed.
Aaron M. Keeler, et al.,
bioRxiv - Biochemistry 2023
Quote:
... gondii tachyzoites in T-150 cm2 cell culture flasks (Corning) in DMEM supplemented with 10% HI-FBS (v:v) ...
-
No products found
because this supplier's products are not listed.
Steven V. John, et al.,
bioRxiv - Cell Biology 2024
Quote:
... gondii surface antigen 1 (SAG1, rabbit, 1:300; Genetex GTX38936) overnight at 4°C and then thoroughly washed with DPBS ...
-
No products found
because this supplier's products are not listed.
Xin Luo, et al.,
bioRxiv - Immunology 2024
Quote:
... of S protein) and recombinant S protein (Sino Biological) were used for stimulation at a final concentration of 2 μg/mL ...
-
No products found
because this supplier's products are not listed.
Justin A. Peruzzi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... purified FLAG tagged protein (recombinant p53 protein, R&D Systems) was run with vesicles to quantify loading ...
-
No products found
because this supplier's products are not listed.
Kylene M. Harold, et al.,
bioRxiv - Physiology 2023
Quote:
... Total protein was stained (Revert Total Protein Stain, LI-COR) and imaged (Odyssey CLx ...
-
No products found
because this supplier's products are not listed.
Dharendra Thapa, et al.,
bioRxiv - Physiology 2019
Quote:
... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
-
No products found
because this supplier's products are not listed.
Eric J. Hartman, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Toxoplasma-infected cells were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in 0.25 M HEPES (ph7.4 ...
-
No products found
because this supplier's products are not listed.
Syrian G. Sanchez, et al.,
bioRxiv - Microbiology 2023
Quote:
... gondii tachyzoites using the Nucleospin RNA II kit (Macherey-Nagel). Reverse transcription was performed with the Superscript III first-strand synthesis kit (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Michael A. Kovacs, et al.,
bioRxiv - Immunology 2022
Quote:
... gondii cysts were enumerated using a DM 2000 LED brightfield microscope (Leica).
-
No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
-
No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.
-
No products found
because this supplier's products are not listed.
Yoshimi Kawamura, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Protein concentrations were measured using BCA Protein Assay Kit (TaKaRa). The samples were subjected to SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Rachel Baum, et al.,
bioRxiv - Cell Biology 2022
Quote:
A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
-
No products found
because this supplier's products are not listed.
Susana M. Contreras, et al.,
bioRxiv - Microbiology 2020
Quote:
... gondii γH2A.X serum sample was obtained from Eurogentec (Belgium) on the basis of the peptide NH2-C+ GKHGV-S(PO3H2)-QEF −COOH ...
-
No products found
because this supplier's products are not listed.
A. Tsitsiklis, et al.,
bioRxiv - Immunology 2020
Quote:
... gondii brain cysts were quantified by imaging of homogenized brain samples stained with fluorescein-conjugated Dolichos biflorus agglutinin (Vector Laboratories) to stain the cell wall with a protocol adapted from (S.-K ...
-
No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...
-
No products found
because this supplier's products are not listed.
Wei-Siang Liau, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein identification was carried out using Protein Pilot 5.0 (SCIEX) for database searching.
-
No products found
because this supplier's products are not listed.
Muaz Nik Rushdi, et al.,
bioRxiv - Immunology 2022
Quote:
... The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
-
No products found
because this supplier's products are not listed.
Stefanie Krug, et al.,
bioRxiv - Immunology 2021
Quote:
... Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Qiuling Wang, Suiwen Hou,
bioRxiv - Plant Biology 2022
Quote:
... Protein concentration was determined using BCA protein assay kit (Solarbio, PC0021).
-
No products found
because this supplier's products are not listed.
Eli Isael Maciel, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
-
No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
-
No products found
because this supplier's products are not listed.
Stefan Kol, et al.,
bioRxiv - Bioengineering 2019
Quote:
... protein A biosensors (Pall) were equilibrated in sample diluent buffer (PBS containing 0.1% BSA and 0.02% Tween-20) ...
-
No products found
because this supplier's products are not listed.
Lalitha Yadhanapudi, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
No products found
because this supplier's products are not listed.
Qiuli Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Izumi Yanatori, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
-
No products found
because this supplier's products are not listed.
Alexandra C. Walls, et al.,
bioRxiv - Immunology 2020
Quote:
... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
-
No products found
because this supplier's products are not listed.
David Gonzalez-Perez, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Proteins were separated by SDS-PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
N Ogino, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
-
No products found
because this supplier's products are not listed.
Isaac W. Babcock, et al.,
bioRxiv - Immunology 2024
Quote:
... gondii strain Me49 was maintained in Swiss Webster mice (#024, Charles River Laboratories) and passaged through CBA/J mice (Jackson Laboratory) ...