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No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated with the anti-Influenza A virus NS1 rabbit antibody (GeneTex; GTX125990) at 1:1000 for two hours ...
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No products found
because this supplier's products are not listed.
Samuel W. Kazer, et al.,
bioRxiv - Immunology 2024
Quote:
... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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No products found
because this supplier's products are not listed.
Michele Martins, et al.,
bioRxiv - Biochemistry 2020
Quote:
... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
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No products found
because this supplier's products are not listed.
Li-Juan Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
Anti-Japanese encephalitis E (mouse monoclonal, Millipore), influenza A H9N2 HA (mouse monoclonal ...
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No products found
because this supplier's products are not listed.
Jonas N. Conde, et al.,
bioRxiv - Microbiology 2023
Quote:
... and recLI9-NS1-GFP11 reporter virus was purified using RNeasy kit (Qiagen). cDNA synthesis was performed as above using random hexamer primers (25°C for 10 min ...
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No products found
because this supplier's products are not listed.
Christoph Ruschil, et al.,
bioRxiv - Immunology 2020
Quote:
... we performed specific enzyme immunoassays to measure IgG-antibodies against tick borne encephalitis in human serum (Immunozym FSME IgG, REF 7701010, PROGEN, Heidelberg, Germany). The ELISA tests were performed according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Javier Gilbert-Jaramillo, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and 1:100 anti-NS1 (Abcam; ab214337) was conducted prior to secondary staining with 1:1000 goat anti-mouse Alexa Fluor 546 and goat anti-rabbit Alexa Fluor 750 ...
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No products found
because this supplier's products are not listed.
S.A. Codeluppi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and pZac2.1 gfaABC1D-lck-GCaMP6f virus (Addgene virus #52924).
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No products found
because this supplier's products are not listed.
Anne Weiß, Ivan Haralampiev, Susann Kummer,
bioRxiv - Microbiology 2022
Quote:
... anti-NS1 (Santa Cruz Biotechnology), and anti-beta-actin (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Romel Rosales Ramirez, Juan E. Ludert,
bioRxiv - Molecular Biology 2019
Quote:
The NS1 sequence from ZIKV (Mexican isolate, Asiatic linkage) and YFV NS1 (Brazilian yellow fever virus isolate) were synthesized de novo (GenScript, Piscataway, NJ). The synthetic ZIKV NS1 gene (GenBank accession number KY631493.1 ...
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No products found
because this supplier's products are not listed.
Chaoyue Zhao, et al.,
bioRxiv - Microbiology 2022
Quote:
... Tick DNA was extracted using the MightyPrep reagent for DNA Kit (Takara, Japan) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Laurie-Anne Lamotte, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... together with Anti-Influenza A Non-Structural Protein 1 (NS1) Mouse Monoclonal Antibody (EMB005, Kerafast). Cells were then washed and incubated with a mixture of secondary antibodies ...
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No products found
because this supplier's products are not listed.
Mathilde Gondard, et al.,
bioRxiv - Epidemiology 2020
Quote:
... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
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No products found
because this supplier's products are not listed.
Amber R. Paulson, et al.,
bioRxiv - Microbiology 2022
Quote:
Tick RNA was assessed using the 2100 Bioanalyzer (Agilent) to ensure sufficient quantity and quality before proceeding to rRNA depletion using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) ...
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No products found
because this supplier's products are not listed.
Nipun Verma, et al.,
bioRxiv - Genetics 2024
Quote:
... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
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No products found
because this supplier's products are not listed.
Nisha Singh, et al.,
bioRxiv - Genetics 2023
Quote:
... Tick cells were then plated on a glass coverslip (Corning). After 72 hours ...
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No products found
because this supplier's products are not listed.
A. A. Blisnick, et al.,
bioRxiv - Animal Behavior and Cognition 2019
Quote:
... and rps4 (a tick housekeeping gene) was evaluated by qPCR using the LightCycler 480 System (Roche). The SyBr green Master mix (Roche ...
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No products found
because this supplier's products are not listed.
Pooja Hoovina Venkatesh, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The JEV NS1 gene was PCR-amplified with Deep Vent polymerase (New England Biolabs) using forward primer OSV 389 and sequential reverse primers OSV 381 ...
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No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ...
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No products found
because this supplier's products are not listed.
Adi Miriam Goldenberg, et al.,
bioRxiv - Neuroscience 2020
Quote:
... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ...
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No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
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No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ...
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No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
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No products found
because this supplier's products are not listed.
Vincent Basse, et al.,
bioRxiv - Microbiology 2021
Quote:
... Purified NS1 was then concentrated using Vivaspin columns (Sartorius).
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No products found
because this supplier's products are not listed.
Agustin Rolandelli, et al.,
bioRxiv - Immunology 2023
Quote:
... Ticks were immobilized on a glass slide using double-sided tape and microinjected (Nanojet III microinjector; Drummond Scientific) through the anal pore with 69 nL of either CLD (Standard macrophage depletion kit ...
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No products found
because this supplier's products are not listed.
Chen-xi Zhang, et al.,
bioRxiv - Immunology 2020
Quote:
The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
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No products found
because this supplier's products are not listed.
Tae Kwon Kim, et al.,
bioRxiv - Systems Biology 2019
Quote:
... ten ticks fully fed but not detached from the host (BD) and six ticks spontaneously detached from the host (SD) ...
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No products found
because this supplier's products are not listed.
Carola Städele,
bioRxiv - Physiology 2023
Quote:
... Ticks were housed in 60 ml aseptic screw cap plastic vials (VWR, 216-1822P) with a mesh-covered 25 mm round hole in the lid ...
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No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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No products found
because this supplier's products are not listed.
Tanya Josek, et al.,
bioRxiv - Zoology 2020
Quote:
... The tip of the second leg on the right side of the tick was excised with surgical microscissors (World Precision Instruments, Sarasota, FL) and connected with an indifferent electrode ...
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No products found
because this supplier's products are not listed.
Jonathan Burnie, et al.,
bioRxiv - Microbiology 2021
Quote:
The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
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No products found
because this supplier's products are not listed.
Elaine Cristina Matos Vicentin, et al.,
bioRxiv - Immunology 2020
Quote:
... of 5 μg of each mAb (αDEC-NS1, αDCIR2-NS1, αDEC or αDCIR2) together with 50 μg poly (I:C) (Invivogen), exactly as described in (17) ...
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No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
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No products found
because this supplier's products are not listed.
Yang Yang, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus titers were measured using p24 enzyme-linked immunosorbent assays (ELISA) (R&D Systems, MN, USA). CD4+ Jurkat T cells were incubated with NL4-3 or NL4-3dNef viruses at MOI ...
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No products found
because this supplier's products are not listed.
Kristen Zarrella, et al.,
bioRxiv - Microbiology 2023
Quote:
... Cells were infected (MOI=1) with WT or UL138STOP virus for 48 hours and then processed for CUT&RUN (Cell Signaling Technologies) as per manufacturer’s recommended protocol ...
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No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
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No products found
because this supplier's products are not listed.
Lok R. Joshi, et al.,
bioRxiv - Immunology 2021
Quote:
... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
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No products found
because this supplier's products are not listed.
Iktae Kim, et al.,
bioRxiv - Biophysics 2022
Quote:
The binding of surface-immobilized NS1 to p85β was measured at 25 °C using an Octet RED biolayer interferometry (Pall ForteBio). The N-terminal His6 and SUMO-tagged NS1 proteins were used for immobilization ...
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No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
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No products found
because this supplier's products are not listed.
Joshua E. McGee, et al.,
bioRxiv - Bioengineering 2023
Quote:
... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
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Recombinant Antigen
Cat# TBEV-NS1-100,
100µg USD $412.0
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Pei Xuan Lee, et al.,
bioRxiv - Microbiology 2020
Quote:
... 10 mg/kg of purified NS1(D2Y98P) (The Native Antigen Company) or OVA protein (InvivoGen ...
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No products found
because this supplier's products are not listed.
Soraia Fernandes, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The frozen specimens were later sectioned into 10 µm tick sections using a cryostat (LEICA CM1950).
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No products found
because this supplier's products are not listed.
L. Rainer Butler, et al.,
bioRxiv - Microbiology 2023
Quote:
... Tick cells were grown to confluence and sub-cultured in capped T25 flasks (Greiner bio-one). All cell cultures were verified by PCR to be Mycoplasma-free (Southern Biotech).
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No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
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No products found
because this supplier's products are not listed.
Kuan-Hung Lin, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and the virus titer was measured based on the titer of the viral protein p24 with QuickTiterTM Lentivirus Titer Kit (Cell Biolabs) following the manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Filippo Bianchini, et al.,
bioRxiv - Immunology 2022
Quote:
... Avi-tagged SARS-CoV-2 RBD and SD1-RBD (both corresponding to SARS-CoV-2 ancestral virus) were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
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No products found
because this supplier's products are not listed.
Takanobu Tagawa, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virus pellet was resuspended to EBM2 (Lonza) media ...
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No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.