-
No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
-
No products found
because this supplier's products are not listed.
L. Rainer Butler, et al.,
bioRxiv - Microbiology 2023
Quote:
... The weight of the ticks was measured using a Pioneer™ analytical balance (OHAUS) and tick engorgement was evaluated ...
-
No products found
because this supplier's products are not listed.
Nikhil J. Parekh, et al.,
bioRxiv - Immunology 2019
Quote:
... Live cells were incubated in Fc block and 10% normal mouse serum (Gemini Bio-Products) at 4°C ...
-
No products found
because this supplier's products are not listed.
Shahrnaz Kemal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All vectors contain an N-terminal 6x-His-tag, C terminal Strep-Tag II tag, with or without a N or C terminal fluorophore (EGFP, mNeonGreen (Allele Biotech), or mRuby2).
-
No products found
because this supplier's products are not listed.
Evangelos Stefanidis, et al.,
bioRxiv - Bioengineering 2023
Quote:
... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
-
No products found
because this supplier's products are not listed.
Luis F. Garcia-Alles, et al.,
bioRxiv - Biochemistry 2019
Quote:
... protein tags were removed by incubation overnight at 25°C with turbo TEV protease (GenWay, 5 μg/mL final) in 50 mM Tris pH 8.0 / 300 mM NaCl /2 mM DTT/ 1 mM EDTA ...
-
No products found
because this supplier's products are not listed.
Martina Hauke, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 10% FCS (PromoCell, Germany). HEK-NF-κB_luc were supplemented for routine growth with 50 µg/mL Hygromycin B (Invivogen ...
-
No products found
because this supplier's products are not listed.
Flavia Chiuppesi, et al.,
bioRxiv - Immunology 2020
Quote:
... or vesicular stomatitis virus G (VSV-G, Aldevron), using FuGENE6 (Roche ...
-
No products found
Akshay Deshpande, et al.,
bioRxiv - Biophysics 2023
Quote:
... 8·104 or 5·105 TZM-bl cells that stably express the emiRFP670-laminB1 nuclear envelope marker was respectively plated in a 8-well chambered slide (#C8-1.5H-N, CellVis) for single time-point nuclear import experiments ...
-
No products found
because this supplier's products are not listed.
Christopher M. Yellman,
bioRxiv - Genetics 2021
Quote:
... The native Nop1 protein was stained with the MCA-28F2 mouse monoclonal antibody (EnCor Biotechnology), followed by anti-mouse CY3 (Jackson ImmunoResearch)
-
No products found
because this supplier's products are not listed.
Maria Jose Lista, et al.,
bioRxiv - Microbiology 2021
Quote:
... The relative quantities of envelope (E) gene were measured using SARS-CoV-2 (2019-nCoV) CDC qPCR Probe Assay (IDT DNA technologies). Relative quantities of E gene were normalised to GAPDH mRNA levels (Applied Bioscience ...
-
No products found
because this supplier's products are not listed.
Binayak Sarkar, et al.,
bioRxiv - Microbiology 2024
Quote:
... while DAGs/TAGs were resolved using a Luna 5U C-5 column (Phenomenex) using established solvent systems.
-
No products found
because this supplier's products are not listed.
Maxime De Rudder, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Factor V serum protein concentration was assessed by ELISA following manufacturer’s instructions (Mouse factor V ELISA kit orb409284, Biorbyt).
-
No products found
because this supplier's products are not listed.
Jiachen Huang, et al.,
bioRxiv - Immunology 2021
Quote:
... 25 µl of secondary antibody (goat anti-human IgG Fc; Meridian Life Science) at a 1:4,000 dilution in blocking buffer was applied to each well for 1 hr at room temperature ...
-
No products found
because this supplier's products are not listed.
Marcin Poreba, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Fluorescent tags (Cyanine-5 NHS and Cyanine-7 NHS) were purchased from Lumiprobe (Hannover, Germany). Diazomethane was generated according to the Aldrich Technical Bulletin (AL-180 ...
-
No products found
because this supplier's products are not listed.
Gila Lustig, et al.,
bioRxiv - Microbiology 2019
Quote:
... Supernatant containing released virus was harvested two days post-transfection and filtered through a 0.45 micron filter(GVS) and stored in 0.5ml aliqouts at −80°C ...
-
No products found
because this supplier's products are not listed.
Yoshiaki Nishimura, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were grown to ~90% confluency in a 96-well plate prior to addition of serially diluted virus to achieve luminescence signals of 30,000-1,000,000 (BMG Labtech PolarStar Optima plate reader at maximum gain) ...
-
No products found
because this supplier's products are not listed.
Joshua D. Powell, et al.,
bioRxiv - Microbiology 2024
Quote:
... NJ) and were seronegative to IAV antibodies by a commercial ELISA kit (Swine Influenza Virus Ab Test, IDEXX) prior to the start of the study ...
-
No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
-
No products found
because this supplier's products are not listed.
Tino Pleiner, et al.,
bioRxiv - Cell Biology 2022
Quote:
... a commercial system with orthogonal cleavage sites based on the SUMOStar tag and SUMOStar protease (LifeSensors, USA) (Liu et al. ...
-
No products found
because this supplier's products are not listed.
Brenton T. Laing, et al.,
bioRxiv - Neuroscience 2022
Quote:
Immunostaining was conducted for axonal projections and verification of virus expression using a chicken anti-GFP polyclonal antibody (1:1000, Cat # GFP-1020, RRID:AB_10000240; Aves Labs, CA, U.S.A.) or a rabbit anti-DsRed polyclonal antibody (1:1000 ...
-
No products found
because this supplier's products are not listed.
Herman KH Fung, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Confocal volume estimation was carried out by ten 1-min FCS measurements of 10 nM Atto488 (ATTO-TEC) in water ...
-
No products found
because this supplier's products are not listed.
Massilia Abbas, et al.,
bioRxiv - Biophysics 2023
Quote:
... MGL-ECDD269H was purified using an AktaXpress with a Strep tag affinity column eluted with 2.5 mM Desthiobiotin followed by a Toyopearl HW-50S gel filtration column (Tosoh Bioscience).
-
No products found
because this supplier's products are not listed.
Danya Abazari, et al.,
bioRxiv - Neuroscience 2022
Quote:
Protein palmitoylation assay was performed using CAPTUREome S-palmitoylated protein kit (Badrilla, Leeds, UK), as described by manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Thaís Del Rosario Hernández, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... The cages also contained a transparent red mouse house (Bio-Serv, mouse arch, red) and a transparent 7-sided pill box for food and water (Amazon ...
-
No products found
because this supplier's products are not listed.
Eleni Kafkia, et al.,
bioRxiv - Systems Biology 2020
Quote:
... mouse anti-lamin B1 (1:500, Atlas Antibodies, AMAb91251) and anti-mouse Alexa Fluor 555 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Joël S. Bloch, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Protein Complex Suite (NeXtal) and PEG/Ion Suite (Hampton Research). Precipitant solution from crystallization screen was dispensed at 1:1 macromolecular complex/precipitant solution ratio in a total drop volume of 400 nL at room temperature ...
-
No products found
because this supplier's products are not listed.
Naureen Javeed, et al.,
bioRxiv - Cell Biology 2019
Quote:
... total protein lysates was isolated using NP-40 buffer (Amresco, J619) supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling Technology ...
-
No products found
because this supplier's products are not listed.
Cassian Afting, et al.,
bioRxiv - Bioengineering 2024
Quote:
WNT surrogate-Fc Fusion Protein (Wnt-surrogate; ImmunoPrecise Antibodies Ltd. ...
-
No products found
because this supplier's products are not listed.
Joanna M. Cooper, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and IV Fc chimera proteins were produced by Molecular Innovations. His-tagged recombinant human tau variants 2N4R ...
-
No products found
because this supplier's products are not listed.
Shan Jiang, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The protein standard with the C-terminal FLAG tag (E-PKSH032870.10) was from Biomol. Linear DNA templates were produced by PCR and were PCR purified ...
-
No products found
because this supplier's products are not listed.
Kishor Dnyaneshwar Ingole, et al.,
bioRxiv - Plant Biology 2020
Quote:
... cDNAs of SUM1AA or SUM3AA were cloned as EcoRI-SalI fragment into pASK-IBA16 vector (N-terminal Strep-tag II affinity tag; Neuromics). For in vitro isoform influence on SUMOylation assays SUM1GG or SUM3GG were cloned as EcoRI-SalI fragment into pASK-IBA16 vector ...
-
No products found
because this supplier's products are not listed.
Laura Medina-Puche, et al.,
bioRxiv - Plant Biology 2019
Quote:
... GFP-fused proteins were detected using mouse monoclonal anti-GFP antibody (1:5,000; Abiocode).
-
No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
-
No products found
because this supplier's products are not listed.
Musleh M. Muthana, et al.,
bioRxiv - Immunology 2023
Quote:
... Azide-free human IgG-Fc was purchased from Athens Research and Technology (Athens ...
-
No products found
because this supplier's products are not listed.
Jinye Liang, et al.,
bioRxiv - Microbiology 2023
Quote:
... and imaged on a Li-COR Odyssey Fc scanner (Carver Center for Genomics ...
-
No products found
because this supplier's products are not listed.
Susan A. Leonhardt, et al.,
bioRxiv - Biophysics 2022
Quote:
... virus-containing supernatants were filtered and placed in poly-lysine-coated glass-bottom dishes (MatTek). Particles were then washed and stained with Alexa594-annexin V (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Valeria Yartseva, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 500 μg BioMag® Goat anti-Human IgG (Fc Specific, Bangs Laboratories #BM562) were washed twice with DPBS without Calcium or Magnesium (Corning #21-031-CV ...
-
No products found
because this supplier's products are not listed.
Yueyuan Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
... All cells were supplemented with 10% (v/v) fetal calf serum (FCS) (ABI) at 37 °C in 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Robert M. Schilke, et al.,
bioRxiv - Immunology 2020
Quote:
... Data analysis was performed using FCS express (Denovo Software) and NovoExpress (Acea Biosciences).
-
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Nila Roy Choudhury, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Cells were incubated with virus for 1.5 hours before the addition of virus growth media (DMEM, 0.14% BSA, 1 μg/mL TPCK-treated trypsin) supplemented with 500 μM of Favipiravir (Selleck Chemicals) or DMSO ...
-
No products found
Larissa Krüger, et al.,
bioRxiv - Microbiology 2023
Quote:
... and the tag was removed with the TEV protease (ratio 10:1 w/w) during overnight dialysis (Biodesign™ Cellulose Dialysis Tubing Roll ...
-
No products found
because this supplier's products are not listed.
Arjen J. Jakobi, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... For the gold labeling experiments the His6-tag was not removed to allow binding of 5 nm Ni-NTA-Nanogold® (Nanoprobes). For consistency the His6-tag was also kept on the proteins for the co-pelletation assay.
-
No products found
because this supplier's products are not listed.
Petra Kangas, et al.,
bioRxiv - Cell Biology 2022
Quote:
... For depletion of the most abundant proteins from CSF ProteoSpin Abundant Serum Protein depletion kit (Norgen Biotek) was used according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Mingyu Fang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... gels were either stained with Coomassie (Protein Ark) or transferred to PVDF membranes for Western blot analysis.
-
No products found
because this supplier's products are not listed.
Jasmin N. Beaver, et al.,
bioRxiv - Neuroscience 2023
Quote:
... all mouse cages contained Nestlets (Ancare, Bellmore, NY) and huts ...
-
No products found
because this supplier's products are not listed.
Qiang Liu, et al.,
bioRxiv - Neuroscience 2024
Quote:
... mouse anti-SaCas9 (Epigentek, A-9001-100, 1:300), mouse anti-GAD67 (Abcam ...
-
No products found
because this supplier's products are not listed.
Kimberley R.G. Cortenbach, et al.,
bioRxiv - Immunology 2021
Quote:
... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Gokul Swaminathan, et al.,
bioRxiv - Immunology 2021
Quote:
... following the Mouse Anti-OVA IgG Antibody Assay Kit (Chondrex), or as previously described (76).
-
No products found
because this supplier's products are not listed.
Michael Korenkov, et al.,
bioRxiv - Immunology 2023
Quote:
For protein complexes crystallization we used a mosquito crystallization robot (TTP Labtech) to set vapor diffusion sitting drops with 96-well iQ plates (TTP Labtech) ...