-
No products found
because this supplier's products are not listed.
Anne L. Sapiro, et al.,
bioRxiv - Microbiology 2023
Quote:
... Ticks were fed on young (4–6-week-old) female C3H/HeJ mice acquired from Jackson Laboratories. Mice were anesthetized with ketamine/xylazine before placement of ≤ 100 larval or ≤ 30 nymphal ticks ...
-
No products found
because this supplier's products are not listed.
Eileen Shiuan, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... samples were blocked in αCD16/32 mouse Fc block (Tonbo Biosciences #70-0161) and stained for extracellular proteins using an antibody master mix made in buffer ...
-
No products found
because this supplier's products are not listed.
Javed Akhter, et al.,
bioRxiv - Microbiology 2020
Quote:
... The recombinant Envelope protein was obtained from MyBioSource (Cat#MBS8309649). All other reagents were from Sigma Aldrich.
-
No products found
because this supplier's products are not listed.
Shih-Chia Yeh, et al.,
bioRxiv - Zoology 2022
Quote:
... and stained using 1:400 mouse monoclonal anti-envelope antibody (4G2) and 1:20,000 secondary anti-mouse Dylight 680 (Rockland). Focus forming units (ffu ...
-
No products found
because this supplier's products are not listed.
Ilhan Tomris, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences). Subsequently ...
-
No products found
because this supplier's products are not listed.
Michael Nshanian, et al.,
bioRxiv - Genetics 2024
Quote:
CUT&Tag analysis of NaBu treated CCD841 cells and mouse intestinal samples were performed using CUT&Tag-IT™ assay kits (Active Motif # 53160, 53170). Approximately 30 mg of tissue was homogenized per reaction by first chopping frozen tissue into smaller pieces in a petri dish with a razor blade on dry ice ...
-
No products found
because this supplier's products are not listed.
Berislav Bošnjak, et al.,
bioRxiv - Immunology 2022
Quote:
... FCS files were analyzed using FCS Express V7 (De Novo Software) or FlowJo V10 (BD).
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No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... Human recombinant ACE2-Fc-tag (Raybiotech) was then added at 1 μg/mL and incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Alexandra Paul, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The envelope vector expressing VSV-G (vesicular stomatitis virus glycoprotein) was obtained from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). To generate the expression plasmid for D614G mutant of SARS-CoV-2 Spike (S ...
-
No products found
because this supplier's products are not listed.
Jiahao Ma, et al.,
bioRxiv - Biophysics 2020
Quote:
... ACE2-Fc (10 μg/mL) was immobilized on Protein A (ProA) biosensors (Pall). Real-time receptor binding curves were obtained by applying the sensor in a two-fold serial dilutions of S-Trimer from 22.5-36 μg/mL in PBS ...
-
No products found
because this supplier's products are not listed.
Agustin Rolandelli, et al.,
bioRxiv - Immunology 2023
Quote:
... Ticks were immobilized on a glass slide using double-sided tape and microinjected (Nanojet III microinjector; Drummond Scientific) through the anal pore with 69 nL of either CLD (Standard macrophage depletion kit ...
-
No products found
because this supplier's products are not listed.
Tess Cherlin, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... and Anti-Mouse (Protein Simple #DM-002) antibodies are used ...
-
No products found
because this supplier's products are not listed.
Carolin Gerke, et al.,
bioRxiv - Microbiology 2023
Quote:
... FCS (PAN Biotech), 1 % (v/v ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
L. Rainer Butler, et al.,
bioRxiv - Microbiology 2023
Quote:
... Tick cells were grown to confluence and sub-cultured in capped T25 flasks (Greiner bio-one). All cell cultures were verified by PCR to be Mycoplasma-free (Southern Biotech).
-
No products found
because this supplier's products are not listed.
Tanya Josek, et al.,
bioRxiv - Zoology 2020
Quote:
... The tip of the second leg on the right side of the tick was excised with surgical microscissors (World Precision Instruments, Sarasota, FL) and connected with an indifferent electrode ...
-
No products found
because this supplier's products are not listed.
Carmen Campos-Silva, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Cytomics FC 500 (Beckman Coulter) or CytoFLEX (Beckman Coulter ...
-
No products found
because this supplier's products are not listed.
S. Kheir, et al.,
bioRxiv - Immunology 2020
Quote:
... cells were seeded in complete DMEM medium (10% FCS, 1000 U/ml Penicillin, 100 µg/ml Streptomycin) containing 30 ng/mL of mouse M-CSF (Peprotech). After two successive medium replacement (at D3 and D6) ...
-
No products found
because this supplier's products are not listed.
Jinchao Chen, et al.,
bioRxiv - Plant Biology 2023
Quote:
... GST-tag Protein Purification Kit (Glutathione Sepharose, BioVision) and MBP-tag Protein Purification Kit (Dextrin Beads ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Yu-Feng Chien, et al.,
bioRxiv - Bioengineering 2020
Quote:
... a TAG lens (TAG lens 2.5β, TAG Optics) was placed directly on top of an objective (UPLFLN 10XP, Olympus) to enable high-speed axial scan of the focus ...
-
No products found
because this supplier's products are not listed.
Jamie C. Moffa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
No products found
because this supplier's products are not listed.
Motokazu Uchigashima, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Tag-blue fluorescent protein (Tag-BFP, Evrogen), Nlgn3Δ ...
-
No products found
because this supplier's products are not listed.
Agustin Rolandelli, et al.,
bioRxiv - Immunology 2023
Quote:
... ticks were immobilized on a glass slide using double-sided tape (3M) and covered in a sphere of 10 µL of L15C300 + 50% FBS ...
-
No products found
because this supplier's products are not listed.
Sarah J. Jefferson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The mouse was briefly anesthetized with isofluorane and the magnetic ear tag was placed on a mouse’s ear using the ear tag applicator (#56791 Stoelting). Each mouse received one ear tag because signal was sufficiently strong with one tag and ears tended to get stuck together when both received ear tags ...
-
No products found
because this supplier's products are not listed.
Zhongyan Lu, et al.,
bioRxiv - Immunology 2021
Quote:
... IE2 and envelope glycoprotein B (Mabtech, OH) at a final concentration of 1 mg/ml for each individual peptide (Supplemental Table 2) ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
-
No products found
because this supplier's products are not listed.
Jennifer L. Reedy, et al.,
bioRxiv - Immunology 2022
Quote:
... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...
-
No products found
because this supplier's products are not listed.
Jerry C. Chang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The plasmids used were mouse full length GSAP with HA tag (EX-Mm30424-M07, Genecopoeia), human GSAP-16k with HA tag (a.a ...
-
No products found
because this supplier's products are not listed.
Mostafa Jarahian, et al.,
bioRxiv - Immunology 2020
Quote:
... Complexes of NCR-Fc fusion proteins (1 to 2 μg per staining) and PE-labeled goat anti-hIgG Fc (Dianova; 1:100 in FACS buffer) were allowed to form for 30 min before being added to cells for 60 min on ice ...
-
No products found
because this supplier's products are not listed.
Sundary Sormendi, et al.,
bioRxiv - Immunology 2020
Quote:
BMDNs were obtained by crushing long bones the bones in 5% FCS using a mortar and either isolated by negative selection using the EasySep Mouse Neutrophil Enrichment Kit (Stemcell Technologies) or by positive selection using biotinylated antibodies (see supplemental data for more details).
-
No products found
because this supplier's products are not listed.
Haoyuan Jing, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Endogenous Bassoon protein was stained by an anti-bassoon mouse monoclonal (Synaptic Systems) antibody ...
-
No products found
because this supplier's products are not listed.
Joshua E. McGee, et al.,
bioRxiv - Bioengineering 2023
Quote:
... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
-
No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
-
No products found
Konstantina Nikolatou, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... V5-Tag (ABM, G189). Following three PFS washes ...
-
No products found
because this supplier's products are not listed.
Andrew B Janowski, et al.,
bioRxiv - Microbiology 2022
Quote:
... the virus RNA was isolated from the virus stocks using Direct-zol RNA MicroPrep (Zymo research) and used for RT-PCR and Sanger sequencing of the entire virus genome.
-
No products found
because this supplier's products are not listed.
Neil G. Rumachik, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... FRG mice were maintained on irradiated high-fat low-protein mouse chow (Envigo Teklad Global 16% Protein Cat#2916) ad libitum to decrease flux through the tyrosine pathway ...
-
No products found
because this supplier's products are not listed.
Pavlo Gilchuk, et al.,
bioRxiv - Immunology 2020
Quote:
A modified protocol using a commercially available human anti-zika virus Envelope protein (ZIKV-Env) IgG kit (Alpha Diagnostics International) was used to quantify ZIKV-117 mAb levels in NHP serum samples ...
-
No products found
because this supplier's products are not listed.
Gary Baisa, et al.,
bioRxiv - Immunology 2020
Quote:
... an RBD-Fc fragment (319-541) with a C-terminal human Fc-tag was purchased from BPS Bioscience (San Diego, CA). Recombinant biotinylated ACE2 was purchased from Acro Biosystems (Newark ...
-
No products found
because this supplier's products are not listed.
Chunru Wei, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The eluted proteins were subjected to immunoblot analysis with anti-FLAG tag polyclonal antibody (Solarbio, China). The detailed Co-IP was performed as described by Zhu and Huq [28].
-
No products found
because this supplier's products are not listed.
Juan Jauregui-Lozano, et al.,
bioRxiv - Genomics 2021
Quote:
CUT&Tag was performed using CUTANA™ CUT&Tag reagents (Epicypher, Durham NC ...
-
No products found
because this supplier's products are not listed.
Manuel Bernabé-García, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and TAG-6 (Calbiochem, #581004) were added to 20 µM and 2.5 µM final concentration in cell culture ...
-
No products found
because this supplier's products are not listed.
Hemakumar M. Reddy, et al.,
bioRxiv - Genomics 2021
Quote:
... Trypsin digested spots were processed to obtain the protein tags by MS analysis on Hybrid Quadrupole TOF mass spectrometer (API QSTAR PULSAR i, PE SCIEX).
-
No products found
because this supplier's products are not listed.
Andrea Toledo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and the V5 tag recombinant Fab fragment (Abnova, RAB00032) were coupled NHS-derived dyes using the same protocol as above but without the size-exclusion chromatography purification step.
-
No products found
because this supplier's products are not listed.
Jenny Y. Pham, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
This study used Escherichia virus T4 (American Type Culture Collection [ATCC] #11303-B4) and three wild-type bacterial hosts ...
-
No products found
because this supplier's products are not listed.
Wanlu Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... TAG (triacylglycerol, D5-TAG-Mix, LM-6000/D5-TAG 17:0,17:1,17:1, Avanti Polar Lipids), PA (phosphatidic acid ...
-
No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Fc receptor blocker (Innovex Biosciences), and finally avidin/biotin blocking buffer (Vector Laboratories ...
-
No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Danila Boytsov, et al.,
bioRxiv - Biophysics 2022
Quote:
... the FCS unit (Confocor3, Carl Zeiss, Jena, Germany) of a commercial laser scanning microscope (LSM 510 ...
-
No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.