-
No products found
because this supplier's products are not listed.
Zuolian Shen, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... thioredoxin (Sigma) was supplied at a final concentration of 0 to 60 μM after 15 min incubation with diamide ...
-
No products found
because this supplier's products are not listed.
Anika Wiegard, et al.,
bioRxiv - Microbiology 2019
Quote:
... vectors containing the GAL4 activation domain (AD) and the GAL4 DNA-binding domain (BD) were used ...
-
No products found
because this supplier's products are not listed.
Sadhana Sharma, et al.,
bioRxiv - Neuroscience 2024
Quote:
... NLR Family Pyrin Domain Containing 3 (NLRP3; 1:1000, Cell Signaling). Membranes were incubated in primary antibody (TBS-T in 5% BSA ...
-
No products found
because this supplier's products are not listed.
Michael B. Geeson, et al.,
bioRxiv - Immunology 2023
Quote:
Antibodies used include: Thioredoxin 1 (ab86255, Abcam; 2429S, Cell Signaling Technology), DPP9 (ab42080 ...
-
No products found
because this supplier's products are not listed.
Aya Zamoto-Niikura, et al.,
bioRxiv - Microbiology 2024
Quote:
Recombinant Bd37 (rBd37) protein was expressed as thioredoxin fusion protein from the pBAD/Thio-TOPO vector (Thermo Fisher Scientific), which encodes thioredoxin and histidine-tag at N-terminus and C-terminus of multi cloning site ...
-
No products found
because this supplier's products are not listed.
Fatima Amanat, et al.,
bioRxiv - Microbiology 2022
Quote:
... proteins from Sino Biologicals (with no trimerization domain) were purchased and used ...
-
No products found
because this supplier's products are not listed.
Ross Gillette, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... zinc finger and BTB domain-containing protein 16 [ZBTB16/PLZF (Santa Cruz Biotechnology, sc-22839, 1:200)] and secondary antibodies Alexa 488 (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Yuki Yoshida, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
-
No products found
because this supplier's products are not listed.
Camille N. Pierre, et al.,
bioRxiv - Immunology 2023
Quote:
... Plasmids encoding N-terminal domain proteins were obtained from GenScript. Plasmids were transiently co-transfected in FreeStyle 293-F cells using 293Fectin (ThermoFisher) ...
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No products found
because this supplier's products are not listed.
Eric S. Nealy, et al.,
bioRxiv - Bioengineering 2023
Quote:
... gBlocks were created for this sequence with a C-terminal “LPETG” Sortase recognition site and complementary 5’ and 3’ overhangs to the BamHI/HindIII double digested pCARSF63 Thioredoxin-SUMO fusion expression plasmid (Addgene #64695).89 The resulting gBlocks were ligated into pCARSF63 expression plasmids using Gibson assembly (NEB ...
-
No products found
because this supplier's products are not listed.
Kinya Nomura, et al.,
bioRxiv - Plant Biology 2023
Quote:
... and a rabbit LexA binding domain antibody (Clontech), respectively.
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No products found
because this supplier's products are not listed.
Manisha Sinha, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
-
No products found
because this supplier's products are not listed.
Daan Vorselen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
-
No products found
because this supplier's products are not listed.
Xinyu Teng, et al.,
bioRxiv - Biophysics 2021
Quote:
... and the flow-through fractions containing the MgtE TM domain protein were concentrated using an Amicon Ultra 50 K filter (Merck Millipore, USA). After concentration ...
-
No products found
because this supplier's products are not listed.
Tasha R Womack, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Rat antibody against Notch 3 intracellular domain was obtained from Biolegend. Blots were probed with a mouse anti-actin (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Jonathan M. Schmitz, et al.,
bioRxiv - Biochemistry 2022
Quote:
... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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No products found
because this supplier's products are not listed.
S Jain, J Maini, A Narang, S Maiti, V Brahmachari,
bioRxiv - Developmental Biology 2019
Quote:
... The recombinant DBINO domain containing protein (~80kDa) was purified using the amylose affinity column (New England Biolabs, Massachusetts, United States) and detected using the anti-MBP-HRP antibody (NEB) ...
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No products found
because this supplier's products are not listed.
Ajay Kumar, et al.,
bioRxiv - Immunology 2021
Quote:
... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
-
No products found
because this supplier's products are not listed.
Seyedehmahsa Moghimi, et al.,
bioRxiv - Microbiology 2022
Quote:
... the FLAG-APEX2 was fused in-frame upstream of the GBF1 truncated after HDS1 domain and containing a BFA-resistant Sec7 domain from ARNO (GARG-1060) in a mammalian expression vector pCI (Promega) generating a plasmid pCI-FLAG-APEX2-GARG- 1060 ...
-
No products found
because this supplier's products are not listed.
Krishnan K. Mahadevan, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... or MRTX849 for 3 hours prior to protein isolation with RIPA buffer containing 1x cOmpleted EDTA free protease inhibitor (Roche) and 1x phosSTOP (Roche) ...
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No products found
because this supplier's products are not listed.
Michal Nemergut, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The filtrated supernatant containing His-thioredoxin-ApoE was applied to a 5 ml Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
-
No products found
because this supplier's products are not listed.
Natasha M. Bourgeois, et al.,
bioRxiv - Systems Biology 2023
Quote:
... Antibodies against DENV non-structural protein 3 (NS3) were obtained from GeneTex (GTX124252), conjugated to Alexa Fluor 647 (NS3-647) ...
-
No products found
because this supplier's products are not listed.
Jonatan Alvelid, Martina Damenti, Ilaria Testa,
bioRxiv - Biophysics 2021
Quote:
... 1 μl of Synaptotagmin-1 antibody (1 mg/ml, luminal domain - Synaptic System, 105 3FB) and 1 μl of FluoTag-X2 anti-mouse KLC (batch n ...
-
No products found
because this supplier's products are not listed.
Bjoern Traenkle, et al.,
bioRxiv - Immunology 2021
Quote:
... VHH domain (Jackson ImmunoResearch) was applied for 15 min ...
-
No products found
because this supplier's products are not listed.
Haoyuan Jing, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 300 mOsm) containing NBQX (3 µM, Tocris) and bicuculine (30 µM ...
-
No products found
because this supplier's products are not listed.
Lingya Yao, et al.,
bioRxiv - Plant Biology 2022
Quote:
... The α-GFP antibody (Abmart) was used to detect the NPR1-YFP protein and α-Histone 3 antibody (Agrisera) was used to detect Histone 3 (as loading control) ...
-
No products found
because this supplier's products are not listed.
Wei Huang, et al.,
bioRxiv - Genetics 2023
Quote:
... 14-3-3 polyclonal antibody (Proteintech, Cat#14503-1-AP), and beta tubulin antibody (Abways Technology ...
-
No products found
because this supplier's products are not listed.
Meredith A. Redd, et al.,
bioRxiv - Physiology 2020
Quote:
... containing 3 μM CHIR99021 (Stem Cell Technologies), 500 μg/mL BSA (Sigma Aldrich) ...
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No products found
because this supplier's products are not listed.
Tierney B. Daw, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Sections were incubated at 4°C for ~3 days in primary antibody solution containing rabbit anti-RFP (Rockland Immunochemicals Inc. ...
-
No products found
because this supplier's products are not listed.
Hisako Takigawa-Imamura, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... containing recombinant human FGF10 protein (PeproTech) was overlaid on explants and allowed to solidify for 15 min at 37°C ...
-
No products found
because this supplier's products are not listed.
Junchao Wei, et al.,
bioRxiv - Bioengineering 2022
Quote:
Spectral-domain OCT (Leica, Chicago, IL, USA), with a broadband superluminescent diode light source (Superlum ...
-
No products found
because this supplier's products are not listed.
Saeko Akiyama, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The antibodies (Tables 2 and 3) were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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No products found
because this supplier's products are not listed.
Juan A. Perez-Bermejo, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Purified 53BP1 Tudor domain was labeled at exposed primary amine groups with NHS-biotin using ChromaLINK NHS-Biotin protein labeling kit (Vector Laboratories). 1 equivalent of chromalink biotin was incubated with the 53BP1 Tudor domain for 2 h and buffer exchanged into fresh PBS ...
-
No products found
because this supplier's products are not listed.
Shahbaz Ahmed, et al.,
bioRxiv - Biophysics 2021
Quote:
... containing chicken anti-HA antibodies from Bethyl labs (1˸600 dilution ...
-
No products found
because this supplier's products are not listed.
Tetyana P. Buzhdygan, et al.,
bioRxiv - Neuroscience 2020
Quote:
... spike protein subunit S2 or receptor binding domain (RBD) sequence of S1 (RayBiotech, Cat No 230-01101 ...
-
No products found
because this supplier's products are not listed.
R. Ryley Parrish, et al.,
bioRxiv - Neuroscience 2023
Quote:
... with mice containing a floxed STOP cassette in front of an eNpHR3.0/EYFP domain (Jackson Laboratory, Stock 014539 ...
-
No products found
because this supplier's products are not listed.
Brien K. Haun, et al.,
bioRxiv - Immunology 2020
Quote:
... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
-
No products found
because this supplier's products are not listed.
Ugo Sardo, et al.,
bioRxiv - Physiology 2023
Quote:
Mouse FGL1 cDNA sequences (full length, N-terminal domain, globular domain) were cloned into pFUSEN-hG2Fc plasmid (InvivoGen) with the following modifications ...
-
No products found
because this supplier's products are not listed.
Susheel Sagar Bhat, et al.,
bioRxiv - Plant Biology 2019
Quote:
... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
-
No products found
because this supplier's products are not listed.
Hyeonji Lim, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The blots were incubated in PBS containing 0.1% Tween-20 and 3% BSA with anti-HIF1α antibody (1:1000) (AF1935, Novus Biologicals) or anti-beta actin antibody (1:1000 ...
-
No products found
because this supplier's products are not listed.
Kevin J. McNaught, et al.,
bioRxiv - Molecular Biology 2020
Quote:
H3K27me2/3 ChIP using anti-H3K27me2/3 antibody (Active Motif, 39536) was performed as previously described (12 ...
-
No products found
because this supplier's products are not listed.
Aditya Iyer, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
-
No products found
because this supplier's products are not listed.
David A. Rocco, Jean-Paul V. Paluzzi,
bioRxiv - Neuroscience 2019
Quote:
... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
-
No products found
because this supplier's products are not listed.
Ankita Walvekar, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 3 ml of 2:1 chloroform: methanol containing different internal standards (Avanti Polar lipids) reported earlier (Abhyankar et al. ...
-
No products found
because this supplier's products are not listed.
Feng Long, et al.,
bioRxiv - Genomics 2020
Quote:
... SMOC2 EC domain and Mut SMOC2 EC domain containing the His tag were synthesized by Bioss Biotechnology ...
-
No products found
because this supplier's products are not listed.
Angela M. Bosco-Lauth, et al.,
bioRxiv - Microbiology 2020
Quote:
... Positive control antibodies to the receptor-binding domain (RBD) and full-length spike protein were human MAb CR3022 antibody (Absolute Antibody, Oxford UK) and human IgG whole molecule (Jackson Immuno Research ...
-
No products found
because this supplier's products are not listed.
Chi-Hong Wu, et al.,
bioRxiv - Neuroscience 2021
Quote:
... the protein-antibody-bead mixtures were resuspended directly in the SDS-containing loading buffer (30 µl, LI-COR) and the Shank3 proteins were eluted into the buffer by heating at 70°C for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Patrik T. Simmler, et al.,
bioRxiv - Cell Biology 2021
Quote:
... SF3B1 antibody from MBL International (D221-3), an HIF1β antibody from Novus Biological (NB 100-124) ...
-
No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli were made from an overnight agar culture and incubated with 3 mM HMGB1 recombinant protein containing a HIS tag (Abclonal) or an hFc tag (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Roger S. Zou, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 3 μL of antibody per IP (Cas9 – Diagenode C15310258 ...