-
No products found
because this supplier's products are not listed.
Wenning Chu, et al.,
bioRxiv - Bioengineering 2023
Quote:
... flowthrough and elution fractions collected as described in Section 2.6 were determined using a HEK 293 HCP ELISA kit (Cygnus Technologies, Southport, NC) following the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Amado Carreras-Sureda, et al.,
bioRxiv - Biochemistry 2021
Quote:
... STIM1 cherry (in HEK-293 cells) or TCR clusters were imaged using a ZET 561/10 excitation filter (Chroma Technology Corp., Bellows Falls, VT). SiR-Actin was measured using 640 nm laser line ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Derek C. Rookhuizen, et al.,
bioRxiv - Immunology 2021
Quote:
... 45µl of room temperature Trans-IT 293 Transfection Reagent (Euromedex, Mirus 2700) was then added and the mixture incubated at RT for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Lars Borgards, et al.,
bioRxiv - Immunology 2023
Quote:
... The assay was performed according to the manufacturer’s instructions with a single technical replicate per sample (Uromodulin Human ELISA, BioVendor R&D, Cat. No.: RD191163200R; Azurocidin ELISA Kit, antibodies-online.com ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
-
No products found
because this supplier's products are not listed.
David M. Zuckerman, et al.,
bioRxiv - Microbiology 2022
Quote:
... The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD, Cocalico, Reamstown, PA; His-PilQOlut, Eurogentec, Seraing, BE). Sera were tested for cross-reactivity by immunoblotting lysates from wildtype M ...
-
No products found
because this supplier's products are not listed.
Luyao Huang, et al.,
bioRxiv - Plant Biology 2023
Quote:
... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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This Fibronectin solution has been purified from human plasma where it is found as a dimer and...
Cat# 5050-1MG,
1 mg, USD $135.0
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Alexei M Bygrave, et al.,
bioRxiv - Neuroscience 2021
Quote:
HEK cells were grown on 10cm plates (for biochemistry) or on collagen (Advanced Biomatrix) coated glass coverslips in 12-well plates (for immunofluorescence and live-cell imaging ...
-
Tezepelumab (Anti-TSLP) is a potent human IgG2 monoclonal antibody that inhibits the binding of...
Cat# A2307, SKU# A2307-1mg*25,
1mg*25, $11370.00
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Gahyang Cho, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... transfected HEK cells were treated with a proteasome inhibitor (20 μM MG132, Selleck Chemicals, TX) for 2 h ...
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Recombinant Antigen
Cat# REC31648-100,
100µg USD $509.0
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Maya Imbrechts, et al.,
bioRxiv - Immunology 2021
Quote:
... Spike Glycoprotein (Full-Length)-His (REC31868-500, The Native Antigen Company). Briefly ...
-
No products found
because this supplier's products are not listed.
Abhinav Parivesh, et al.,
bioRxiv - Cell Biology 2022
Quote:
HEK-293T cells were seeded at 2.5×106 in 25ml of DMEM (Genesee Cat. 25-500) completed with 10% heat-inactivated FBS (Thermo Cat ...
-
No products found
because this supplier's products are not listed.
Kari H. Ecklund, et al.,
bioRxiv - Cell Biology 2021
Quote:
Anti-His-coated 0.44 μm microbeads (PSS4; Spherotech; prepared as described previously72) were incubated with purified 6His-GFP-3HA-GST-dynein331-HALO in dynein trapping buffer (30 mM HEPES pH 7.2 ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
-
No products found
because this supplier's products are not listed.
Thomas Klünemann, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Cryoprotection was achieved by washing in reservoir solution containing 10% of (R,R)-2,3-butandiol (Alfa Aesar) for a few minutes prior to flash-cooling in liquid nitrogen.
-
No products found
because this supplier's products are not listed.
Sabal Chaulagain, et al.,
bioRxiv - Microbiology 2020
Quote:
... and imaged with FluorChem R (Protein Simple). Densitometric analysis was performed using ImageJ software and all readings were normalized to GAPDH values.
-
No products found
because this supplier's products are not listed.
Shekinah Phillips, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and R-CPP (5 μM; Hello Bio). Recordings were performed at a 10 mV test pulse at the end of each sweep to monitor series resistance and was excluded if there was more than a 20% change during the experiment ...
-
No products found
because this supplier's products are not listed.
Xiaoyu Shi, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and HEK 293T cells were seeded at 1 × 104 cells/cm2 in 16-well chambers (Grace Bio-Labs, 112358) and grown to 75% confluency.
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293 cells
Cat# HY29315,
1 x 1,5mL Hype-293 + 1 x 5mL B293, USD $197.00/KIT
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Saritha S. D’Souza, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 30μl of ViroMag R/L beads (OZ Biosciences) were added and incubated at room temperature for 15 minutes.35 During incubation 3×105 wild-type or CCR5-mut cells were placed in a single well of a 24-well plate and centrifuged at 530g for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Adrienne E. D. Stormo, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... the protocol is as described in Stehbens et al80 except that the system was upgraded with a next generation scientific CCD camera (cMyo, 293 Photometrics) with 4.5 um pixels allowing optimal spatial sampling using a 60x NA 1.49 objective (CFI 294 APO TIRF ...
-
No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
-
No products found
because this supplier's products are not listed.
Liam Hudson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... C-His tagged IDH1 R132H (Met1-Leu414) was purchased from G-Biosciences (BAN1708, 50 µg). N-His and GST tagged USP7 (Lys208-Glu560 ...
-
No products found
because this supplier's products are not listed.
Yoichi Araki, et al.,
bioRxiv - Neuroscience 2023
Quote:
Live HEK 293T cells and cultured hippocampal neurons were imaged using a Cell Observer spinning disk confocal microscope (Carl Zeiss) or an LSM 880 laser scanning confocal microscope (Carl Zeiss) ...
-
No products found
because this supplier's products are not listed.
Tracy J. Berg, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Primary human astrocytes (3H Biomedical) were cultured in Astrocyte Medium (3H Biomedical ...
-
No products found
because this supplier's products are not listed.
Peeali Mukherjee, et al.,
bioRxiv - Biophysics 2023
Quote:
... Crystals of FlrB-PAS7-108 were obtained by hanging drop vapour diffusion method at 293 K using 24 well crystallization tray with Ammonium Sulfate Grid Screen from Hampton Research with protein of concentration 10 mg ml-1 ...
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No products found
because this supplier's products are not listed.
Bradley M. Roberts, et al.,
bioRxiv - Neuroscience 2020
Quote:
... rabbi anti-NeuN (1:500, Biosensis, R-3770-100); guinea pig anti-S100β (1:2,000 ...
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No products found
because this supplier's products are not listed.
Monique Merchant, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... (S,R) Bis(Monoacylglycero)Phosphate (BMP) (Avanti Polar Lipids), and 1-cholesterol (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Daniel Schindler, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... The DNA Hi-C libraries were sheared into 300 bp using a Covaris S220 apparatus (Covaris) and the biotin-labeled fragments were selectively captured by Dynabeads Myone Streptavidin C1 ...
-
No products found
because this supplier's products are not listed.
Tal M. Dankovich, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and mouse anti-Tenascin-R (1:200, Synaptic Systems, 400004) for 24h ...
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No products found
because this supplier's products are not listed.
Lior Tal, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Arabidopsis KAI2 protein was expressed as a 6× His-SUMO fusion protein using the expression vector pSUMO (LifeSensors). His-SUMO-KAI2 was isolated from by Ni-NTA resin and the eluted His-SUMO KAI2 was further separated by anion-exchange ...
-
No products found
because this supplier's products are not listed.
Thi K. Tran-Nguyen,
bioRxiv - Molecular Biology 2021
Quote:
Recombinant human GRP78s purchased from StressMarq Biosciences or produced in the laboratory [30] were used as antigens in ELISA ...
-
No products found
because this supplier's products are not listed.
Nazila V. Jafari, Jennifer L. Rohn,
bioRxiv - Microbiology 2022
Quote:
HBLAK human bladder progenitor cells (CELLnTEC, Switzerland) were grown according to the CELLnTEC protocol ...
-
No products found
because this supplier's products are not listed.
Sei Motouchi, et al.,
bioRxiv - Biochemistry 2023
Quote:
... the samples were loaded onto the Sciex X500 R QTOF (Sciex) in the positive mode at a 20 μL/min flow rate.
-
No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
-
No products found
because this supplier's products are not listed.
Maurizio Chioccioli, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... using human Col1a1 and Acta2 primers from ABI for the specified gene and species.
-
No products found
because this supplier's products are not listed.
Yuichi Mitsui, et al.,
bioRxiv - Immunology 2022
Quote:
Human PBMCs were purchased from Precision for Medicine, Inc ...
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No products found
Alex M. Jaeger, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
-
No products found
because this supplier's products are not listed.
Léa Paolini, et al.,
bioRxiv - Immunology 2024
Quote:
... The data was then analyzed using R software and the phenoptrReports package (Akoya).
-
No products found
because this supplier's products are not listed.
Ava P. Soleimany, et al.,
bioRxiv - Bioengineering 2020
Quote:
Fresh frozen human PCa TMAs (BioChain Institute, Inc.; T6235201) were air dried and fixed in ice-cold acetone for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Diana R. Dou, et al.,
bioRxiv - Immunology 2021
Quote:
... and mouse anti-human CD4 (Tonbo Biosciences, 20-0048-T025), and sorted for live 7-AAD- CD3+ CD4+ T-lymphocytes using the BDFACS ARIA II with a 100 uM nozzle ...
-
No products found
because this supplier's products are not listed.
Mutsumi Kobayashi, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Human iPSCs were dissociated using Accutase (Innovative Cell Technologies, AT104) and suspended in mTeSR plus (Stemcell Technologies ...
-
No products found
because this supplier's products are not listed.
Huu Tuan Nguyen, et al.,
bioRxiv - Bioengineering 2023
Quote:
Human umbilical vein endothelial cells (ECs, Angio-Proteomie, MA, USA) were cultured in Vasculife (LifeLine Cell Technology ...
-
No products found
because this supplier's products are not listed.
Inci Ramírez-Bello, et al.,
bioRxiv - Microbiology 2024
Quote:
... A secondary anti-human IgG coupled to peroxidase (Aviva SB) was added at a dilution of 1:1,000 in PBS.
-
No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... I-FABP (Human I-FABP ELISA Kit, Hycult biotech, Cat# HK406), and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit ...
-
No products found
because this supplier's products are not listed.
Syed M. Moin, et al.,
bioRxiv - Immunology 2022
Quote:
Purified H10ssF was applied to glow discharged Quantifoil R 2/1 300 mesh grids (Ted Pella, Redding) at a concentration of 1 mg ml-1 ...
-
No products found
because this supplier's products are not listed.
Julian Schöllkopf, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
N-terminally His-tagged Pasteurella multocida toxin (PMT) was coated at 10 µg/mL (500 ng/well ...
-
No products found
because this supplier's products are not listed.
Surbhi Verma, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human MDMs (hMDMs) were isolated from human PBMCs and separated from healthy human donors’ blood using PolymorphPrep (PROGEN,1895) layering and centrifugation ...
-
No products found
because this supplier's products are not listed.
Marcel Rak, et al.,
bioRxiv - Biochemistry 2023
Quote:
Co-crystallization trials were performed using the sitting-drop vapor-diffusion method at 293 K with a mosquito crystallization robot (TTP Labtech, Royston UK). MST3 (11 mg/mL in 25 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Tommy Tong, et al.,
bioRxiv - Immunology 2023
Quote:
... followed by anti-human IgG AP conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT ...
-
No products found
because this supplier's products are not listed.
Piyakarn Boontem, Tetsumori Yamashima,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... rabbit anti-human activated μ-calpain antibody (PEPTIDE Institute, Japan) at 1:250 overnight ...