-
No products found
because this supplier's products are not listed.
Bodie Curren, et al.,
bioRxiv - Immunology 2022
Quote:
... TSLP (Biolegend, San Diego, CA) and CXCL1 (aka KC ...
-
No products found
because this supplier's products are not listed.
Mona O. Mohsen, et al.,
bioRxiv - Immunology 2021
Quote:
... anti-mouse CD16/CD32 (mouse BD Fc block) mAb clone 2.4G2 (BD Bioscience ...
-
No products found
because this supplier's products are not listed.
Agnieszka M. Kabat, et al.,
bioRxiv - Immunology 2022
Quote:
... or TSLP (R&D Systems, #555-TS, 50ng/ml) where indicated ...
-
No products found
because this supplier's products are not listed.
Quentin Marquant, et al.,
bioRxiv - Immunology 2020
Quote:
... Thymic stromal lymphoprotein (TSLP) (all Ready-Set-Go!® ELISA, ThermoFisher Scientific), and KC/CXCL1 (R&D Systems) ...
-
No products found
because this supplier's products are not listed.
AJ Voss, et al.,
bioRxiv - Neuroscience 2022
Quote:
... TSLP (Sino Biological, Cat. 16135-H08H, 20ng/mL), DKK1 (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Wei Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... bound ACE2-mouse Fc was detected by HRP-conjugated anti mouse IgG (Fc specific) (Sigma-Aldrich). Another way to do hACE2 competition ELISA is to incubate the fixed concentration of biotinylated hACE2-Fc with gradient concentrations of antibody ...
-
No products found
because this supplier's products are not listed.
George N. Llewellyn, et al.,
bioRxiv - Microbiology 2023
Quote:
... mouse-anti-human IgG Fc-HRP (Southern Biotech) was diluted 1:2000 in blocking buffer and added and plates were incubated for 2 hours at 370C ...
-
No products found
because this supplier's products are not listed.
Annette B. Vogel, et al.,
bioRxiv - Immunology 2020
Quote:
... Anti-mouse-Fc-antibody (Jackson ImmunoResearch) was diluted in 10 mM sodium acetate buffer pH 5 (30 µg/mL ...
-
No products found
because this supplier's products are not listed.
Nikhil Khatwani, et al.,
bioRxiv - Immunology 2023
Quote:
... anti-mouse FC blocking Ab (Bioxcell) was applied on ice for 15 minutes to samples prepared as described above ...
-
No products found
because this supplier's products are not listed.
Susanta Chatterjee, et al.,
bioRxiv - Molecular Biology 2021
Quote:
TET-ON stable HEK293 cells with the Tetracycline-inducible expression constructs were grown in DMEM supplemented with 10% TET-approved FCS (Clontech) and induction of expression of respective gene product was carried out for indicated time durations using 400 ng/ml Doxycycline (SIGMA) ...
-
No products found
because this supplier's products are not listed.
Shixiu Liang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... anti-TSLP (abcam, ab188766), anti-IL-25 (abclonal ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... HEK293-hTLR7 (Invivogen hkb-htlr7), HEK-Lucia™ hRIG-I (Invivogen hkl-hrigi) ...
-
No products found
because this supplier's products are not listed.
K.K. Khoo, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Sangsin Lee, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and mouse IgG1 Fc (Addgene #28216) and IgG2a Fc (Addgene #114492) ...
-
No products found
because this supplier's products are not listed.
Sophie D. Adams, et al.,
bioRxiv - Cell Biology 2020
Quote:
... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Christian L. Egly, et al.,
bioRxiv - Biochemistry 2024
Quote:
... All HEK293 cells were cultured in HEK293 media consisting of Minimum Essential Media (MEM, Corning) containing 10% (v/v ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Shingo Hanaoka, Shinji Saijou, Yasuhiro Matsumura,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... reacted with HRP-conjugated anti-human IgG-Fc antibody or anti-mouse IgG-Fc antibody (Bethyl) for 1 hour ...
-
No products found
because this supplier's products are not listed.
Sonal Jaiswal, Srishti Sanghi, Priyanka Singh,
bioRxiv - Cell Biology 2023
Quote:
... and anti-mouse IgG (Fc) TRITC (Merck, #SAB3701020, 1:500).
-
No products found
because this supplier's products are not listed.
Andrew M Glazer, et al.,
bioRxiv - Genetics 2019
Quote:
... or HEK293 cells using Fugene 6 (Promega) following manufacturer’s instructions and studied 48-72 hours post-transfection.
-
No products found
because this supplier's products are not listed.
Terence C. Theisen, John C. Boothroyd,
bioRxiv - Microbiology 2021
Quote:
... Nextera XT indices were used to generate libraries from 1 ng of cDNA material for each sample following the instructions provided by Illumina (Illumina FC-131-1096 and FC-131-2001). Library preparations were purified using AMPure XP beads and checked for quality using the Agilent Bioanalyzer at Stanford Functional Genomics Facility (SFGF) ...
-
No products found
because this supplier's products are not listed.
P.K Smitha, et al.,
bioRxiv - Molecular Biology 2022
Quote:
IgGFc: Human IgG Fc Sequence (Supplementary Material Figure S1) was codon optimized for HEK293 expression and synthesized from Genscript USA in pUC57 vector ...
-
No products found
because this supplier's products are not listed.
Laurent Gros, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cells were incubated with mouse Fc-blocking reagent (Miltenyi Biotec) plus Viakrom-808 (Bekman Culter ...
-
No products found
because this supplier's products are not listed.
Pengfei Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
-
No products found
because this supplier's products are not listed.
Junfeng Gao, et al.,
bioRxiv - Immunology 2023
Quote:
... TSLP (50 ng/mL, 300-62, PEPROTECH, Cranbury, NJ) or IL-25 (50 ng/mL ...
-
No products found
because this supplier's products are not listed.
Eileen Shiuan, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... samples were blocked in αCD16/32 mouse Fc block (Tonbo Biosciences #70-0161) and stained for extracellular proteins using an antibody master mix made in buffer ...
-
No products found
because this supplier's products are not listed.
Martin P. Schwalm, et al.,
bioRxiv - Biochemistry 2023
Quote:
HEK293 cells were cultured in DMEM (PAN Biotech). All media were supplemented in with 10% FBS (PAN Biotech ...
-
No products found
because this supplier's products are not listed.
Andrea J. Pruijssers, et al.,
bioRxiv - Microbiology 2020
Quote:
... or 10% FCS fetal clonal serum (FCS)(HyClone, GE Life Sciences), 100 U/ml penicillin and streptomycin (Gibco ...
-
No products found
because this supplier's products are not listed.
Yun-Ruei Kao, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Signals were visualized and collected by LI-COR Odyssey Fc (LI-COR Biosciences). ImageJ (https://imagej.nih.gov/ij/ ...
-
No products found
because this supplier's products are not listed.
Li Xu, et al.,
bioRxiv - Bioengineering 2020
Quote:
Genomic DNA from mouse tissues and cultured HEK293 cells were extracted using DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD). Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research ...
-
No products found
because this supplier's products are not listed.
Berislav Bošnjak, et al.,
bioRxiv - Immunology 2022
Quote:
... FCS files were analyzed using FCS Express V7 (De Novo Software) or FlowJo V10 (BD).
-
No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Kuan-Ting Huang, et al.,
bioRxiv - Biochemistry 2022
Quote:
Fluorescence stereomicroscope (e.g., Leica M165 FC)
-
No products found
because this supplier's products are not listed.
Xiao Ma, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... HEK293/GC-A+ and HEK293/GC-B+ cell lines were generated from HEK293 parental cell transfected with plasmids (OriGene, Rockville, MD) containing either human GC-A or GC-B cDNA sequences ...
-
No products found
because this supplier's products are not listed.
Luciana N García, et al.,
bioRxiv - Cell Biology 2019
Quote:
... TSLP (1:200- Gene Tex, USA) or pEGFR (1:50 - Santa Cruz Biotechnology), with bound antibodies being detected using anti-rabitt (for SP-D ...
-
No products found
because this supplier's products are not listed.
Nicolò Maganzini, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Mouse Fc-capture biosensor tips were purchased from Sartorius. Chicken plasma (cat # ...
-
No products found
because this supplier's products are not listed.
Andre Watson, et al.,
bioRxiv - Bioengineering 2020
Quote:
ACE2-HEK293s (BPS Bioscience) were cultured and transduced in opaque 96-well white plates (Corning® ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Bert Schepens, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Izumi Sasaki, et al.,
bioRxiv - Immunology 2023
Quote:
... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
-
No products found
because this supplier's products are not listed.
Saskia D. van Asten, et al.,
bioRxiv - Immunology 2020
Quote:
... 1% FCS and 12.5µg/ml DNAse (Roche) in IMDM (Gibco) ...
-
No products found
because this supplier's products are not listed.
Jordana Griffiths, et al.,
bioRxiv - Immunology 2020
Quote:
... For domain binding determination PE-labelled secondary anti-mouse Fc antibody (Jackson Laboratories) was added ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Stephan Steinke, et al.,
bioRxiv - Neuroscience 2022
Quote:
The three NMDAR fusion protein constructs were subcloned into pCSE2.7-mIgG2a-Fc-XP (N1-Fc and N2B-Fc) or pCSE2.8-mIgG2a-Fc-Xp (N1-N2B-Fc) using NcoI/NotI (New England Biolabs, Frankfurt, Germany) for mammalian production in Expi293F cells ...
-
No products found
because this supplier's products are not listed.
Luciana N García, et al.,
bioRxiv - Cell Biology 2019
Quote:
... with bound antibodies being detected using anti-rabitt (for SP-D, TNFα, TSLP and CCSP) or anti-goat (for TLR4 and pEGFR) biotin-labeled antibodies (Vector Laboratories, Burlingame, CA, USA) in 1% PBS-BSA ...
-
No products found
because this supplier's products are not listed.
Hangu Nam, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Fc Tag (ACROBiosystems). To obtain comparable live cell counts between conditions ...
-
No products found
because this supplier's products are not listed.
Jaehyeon Jeong, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... HEK293 lysates were collected using RIPA buffer (Cell Signaling Technology), and the protein concentrations of the lysates were quantified through Bradford assays (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The device was presoaked in FC-40 (3M) for more than 30 min and placed in a stage top incubator (STXG-WSKMX ...