-
No products found
because this supplier's products are not listed.
Alexandra Tsirigotaki, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
-
No products found
because this supplier's products are not listed.
Yong Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
-
No products found
because this supplier's products are not listed.
Gábor Tax, et al.,
bioRxiv - Cell Biology 2023
Quote:
... An anti Trop-2 mAb (T16) and and anti Trop-2 pAb (AF650 from R&D Systems) were used to perform staining of live ...
-
No products found
because this supplier's products are not listed.
Filip Mihalic, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
-
No products found
because this supplier's products are not listed.
Gábor Tax, et al.,
bioRxiv - Cell Biology 2023
Quote:
... UGGT2-/- and UGGT1/2-/-cells were transfected with 1 µg Trop-2-pEYFP-N1 or Trop-2-Q118E-pEYFP-N1 plasmids using X-tremeGENE 9 DNA transfection reagent (Roche, Basel, Switzerland) following the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Alexander Kuzin, et al.,
bioRxiv - Genomics 2021
Quote:
... human Hek293 DNA was purchased from Genscript. S ...
-
No products found
because this supplier's products are not listed.
Aswini Panigrahi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... from the expression vector pcDNA3.1/Myc-His(-)-HSulf-2 bearing human Sulf-2 cDNA (a gift from Steven Rosen, Addgene plasmid # 13004) (Morimoto-Tomita et al. ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Ivan K. Domingo, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... HEK293-Null2 cells were also co-transfected with a human MD-2 expression clone (OriGene, RC204686). JetPRIME (Polyplus ...
-
No products found
because this supplier's products are not listed.
Maria Lange Pedersen, et al.,
bioRxiv - Immunology 2022
Quote:
... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
-
No products found
because this supplier's products are not listed.
Man Zhou,
bioRxiv - Biophysics 2021
Quote:
... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
No products found
because this supplier's products are not listed.
Zachary R. Crook, et al.,
bioRxiv - Bioengineering 2024
Quote:
... biotinylated His-Avi-tagged human TfR (TfR cross-reactivity, ACROBiosystems TFR-H82E5); His-tagged mouse TfR (TfR cross-reactivity ...
-
No products found
because this supplier's products are not listed.
Tzu-Hsuan Chang, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
-
No products found
because this supplier's products are not listed.
Jillian M. DiMuzio, et al.,
bioRxiv - Immunology 2021
Quote:
... HEK293 cells expressing human Angiotensin converting enzyme 2 (ACE2) (BPS Biosciences, Cat #79951) were cultured in EMEM containing 10% FBS and 5 mg/mL Puromycin to select for ACE2-expressing cells ...
-
No products found
because this supplier's products are not listed.
Jessica Gomez-Garrido, et al.,
bioRxiv - Genomics 2023
Quote:
... and Hi-C (Illumina NovaSeq 6000, 2×150bp) for chromosome-level scaffolding (Fig ...
-
No products found
because this supplier's products are not listed.
Chloe G Myers, et al.,
bioRxiv - Cell Biology 2024
Quote:
Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Wenjuan Yang, et al.,
bioRxiv - Immunology 2021
Quote:
... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
-
No products found
because this supplier's products are not listed.
Qiming Liu, et al.,
bioRxiv - Plant Biology 2022
Quote:
... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
-
No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ...
-
No products found
because this supplier's products are not listed.
Nilabhra Mitra, Sanghamitra Dey,
bioRxiv - Plant Biology 2021
Quote:
Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Bashir A. Ali, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or anti-His (Cell Signaling #2366 His-Tag 27E8) primary antibodies ...
-
No products found
because this supplier's products are not listed.
Hoang Nguyen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
-
No products found
because this supplier's products are not listed.
Qing Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01 ...
-
No products found
because this supplier's products are not listed.
Catherine Horiot, et al.,
bioRxiv - Immunology 2023
Quote:
... recombinant human IL-6 (2 ng/mL) (Proteintech) was added to RPMI1640 culture medium containing Ultraglutamine (Lonza ...
-
No products found
because this supplier's products are not listed.
Md. Alamgir Hossain, et al.,
bioRxiv - Immunology 2022
Quote:
... Anti-human IgM F(ab’)2 (Jackson ImmunoResearch) was also included in the antigen plate ...
-
No products found
because this supplier's products are not listed.
Karuna Ganesh, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... rabbit anti-human Muc-2 (Santa Cruz, cat. #15334), and rabbit anti-human Ki67 (abcam ...
-
No products found
because this supplier's products are not listed.
Ivan Kosik, et al.,
bioRxiv - Microbiology 2023
Quote:
... Goat F(ab)2 anti-human IgG: HRP (Biorad), Goat anti-mouse IgG (H/L) ...
-
No products found
because this supplier's products are not listed.
Pengfei Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
-
No products found
because this supplier's products are not listed.
Jianan Zhang, et al.,
bioRxiv - Biophysics 2024
Quote:
... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Chong Li, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries were generated with 1.5 M human cells as input using Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) according to the manufacturer’s protocol with the following change ...
-
No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
No products found
because this supplier's products are not listed.
Lih-Yun Hsu, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
-
No products found
because this supplier's products are not listed.
Masako Kohyama, et al.,
bioRxiv - Microbiology 2022
Quote:
... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Ruby Schipper, et al.,
bioRxiv - Cell Biology 2024
Quote:
... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
-
No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
-
No products found
because this supplier's products are not listed.
Alena Casella, et al.,
bioRxiv - Bioengineering 2023
Quote:
Human microvascular endothelial cells (HMVECs) were cultured using Endothelial Growth Media 2 (EGM-2, PromoCell, Heidelberg Germany) prior to staining with CellTrackerTM dye (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Efil Bayam, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 2% glucose and the appropriate –His dropout mix (CSM-His, MP Biomedicals) or in autophagy induction medium SD-N ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Nayab Fatima, et al.,
bioRxiv - Neuroscience 2020
Quote:
Primary human astrocytes (passage 2-7) obtained from ScienCell were cultured in astrocyte medium (ScienCell ...
-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Maciej Kujawski, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Murine anti-human CD3 (OKT3; InVivoMAb, BE0001-2) was from BioXCell (Lebanon, NH), and rat anti-murine CD3 (Cat no 100331 ...