-
No products found
because this supplier's products are not listed.
Liisa Nissinen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HEK and HOK (human oral keratinocytes) were from ScienCell Research Laboratories (Carlsbad ...
-
No products found
because this supplier's products are not listed.
Cary R. Boyd-Shiwarski, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
-
No products found
because this supplier's products are not listed.
Maryna V. Ivanchenko, et al.,
bioRxiv - Neuroscience 2023
Quote:
Total RNA was extracted from transduced HEK 293 cells using the Zymo Quick-RNA Microprep Kit (Zymo Research, #R1050). Reverse transcription was performed with Invitrogen’s SuperScript IV VILO Master Mix with ezDNAse enzyme (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Inmaculada Sanjuan-Ruiz, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Rabbit anti-FUS 293 (Bethyl, A-300-293A, 1/2000), Rabbit anti-FUS 294 (Bethyl ...
-
No products found
because this supplier's products are not listed.
Alexander P. Clark, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human embryonic kidney cells 293 stably expressing human hyperpolarization-gated cyclic nucleotide-sensitive cation channel 1 (HEK-HCN1) were obtained from Charles River (CT6114). Cells were cultured and maintained according to the online protocol by Charles River ...
-
No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
... His-tagged recombinant human AKT1 (BPS Bioscience, Cat# 40003), AKT2 (BPS Bioscience ...
-
No products found
because this supplier's products are not listed.
Patrick Blatt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Rabbit anti-His (1:000, Rockland Inc., 600-401-382), Mouse anti-Fibrillarin (1:25 ...
-
No products found
because this supplier's products are not listed.
Lie Wang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
-
No products found
because this supplier's products are not listed.
Seána Duggan, et al.,
bioRxiv - Microbiology 2019
Quote:
Human neutrophil defensin-1 (hNP-1) (AnaSpec Incorporated, California, USA) susceptibility assay was performed as described previously (15) ...
-
No products found
because this supplier's products are not listed.
Opeyemi S. Adeniji, et al.,
bioRxiv - Microbiology 2021
Quote:
... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
-
No products found
because this supplier's products are not listed.
Raphael Fettig, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... NIH-3T3 fibroblasts and HEK-293 cells (all from ATCC, LGC Standards GmbH, Wesel, Germany) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Taisei Wakigawa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... BAY-293 (Tocris, 6857, 1 μM), LY294002 (Sigma‒Aldrich ...
-
No products found
because this supplier's products are not listed.
Juan A. Ortega, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Image acquisition for HEK-293 experiments was performed on a Leica DMI4000B laser scanning confocal microscope (Leica, Buffalo Grove, IL) or with Leica DMi8 microscope (Leica ...
-
No products found
because this supplier's products are not listed.
Vilde Leipart, et al.,
bioRxiv - Biochemistry 2022
Quote:
... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
-
No products found
because this supplier's products are not listed.
Xinrui Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His-tone H2A.Z (1:200, Active Motif #39013), Nkx2.5 (1:250 ...
-
No products found
because this supplier's products are not listed.
Eri Takematsu, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Viruses were produced in HEK-293Ta cells using human lentiviral packaging system according to the manufacturer’s instructions (Genecopoeia). Puromycin was used to select only for transduced cells ...
-
No products found
because this supplier's products are not listed.
Bekir Altas, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
-
No products found
because this supplier's products are not listed.
NC Rodgers, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Solutions of 434 nM His-CLASP2α or 1 μM α-actinin (Cytoskeleton, Inc.) were incubated with and without 15 μM phalloidin-stabilized F-actin for 20 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
D. Azimova, et al.,
bioRxiv - Microbiology 2021
Quote:
... mouse anti-His (ABclonal AE003).
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Megan E. Huber, et al.,
bioRxiv - Immunology 2022
Quote:
... α-human IFN-γ (7-B6-1, MabTech); for fluorescence microscopy ...
-
No products found
because this supplier's products are not listed.
Ethan Iverson, et al.,
bioRxiv - Microbiology 2021
Quote:
... recombinant human IFNβ (1 nM, 11415-1, PBL Assay Science), IFNλ3 (10 nM ...
-
No products found
because this supplier's products are not listed.
Eleonora Grisard, et al.,
bioRxiv - Cell Biology 2021
Quote:
... rabbit anti-human Lamp1 1/1000 (clone EPR4204, GeneTex), mouse anti-Gapdh (clone 1E6D9 ...
-
No products found
because this supplier's products are not listed.
Miquel Rosas-Salvans, et al.,
bioRxiv - Cell Biology 2021
Quote:
... CREST (1:100, human, Antibodies Incorporated, 15-234-0001), Hec1 (1:200 ...
-
No products found
because this supplier's products are not listed.
Sandra D. Chanez-Paredes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse anti-6-His (Bio X Cell, AB_2687790) was used for western blot (3 µg/ml) ...
-
No products found
because this supplier's products are not listed.
Magda Krakowczyk, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HEK-293 cells (60%-70% of confluency) were fixed by 2.5% glutaraldehyde and 2% paraformaldehyde (both from Electron Microscopy Science, Inc.) solution for 1 h at 4°C ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Maxime Lorenzini, et al.,
bioRxiv - Physiology 2020
Quote:
Whole-cell NaV currents were recorded at room temperature from transiently transfected HEK-293 cells using an Axopatch 200A amplifier (Axon Instruments, Molecular Devices, San Jose, CA) 48 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
-
No products found
Tamara Miljuš, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
For all experiments human embryonic kidney (HEK) 293SL cells84 were used and regularly tested for mycoplasma contamination (PCR Mycoplasma Detection kit, ABM, Canada). HEK293SL cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
-
No products found
because this supplier's products are not listed.
Madison A. DeWinter, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... his-tagged 25 μg/ml GLP-1 receptor (GLP-1 R MyBioSource.com MBS949592) and 50 μg/ml parathyroid hormone receptor (PTH ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Kui K. Chan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and anti-HIS-FITC (chicken polyclonal, 1/100 dilution; Immunology Consultants Laboratory) secondary antibodies for 20 minutes at 4°C ...
-
No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
No products found
because this supplier's products are not listed.
Yongping Ma, et al.,
bioRxiv - Immunology 2021
Quote:
... 100 µl of rabbit anti-His-tag antibody (1:1000, BioVision, USA, cat#3998) was incubated at 37℃ for 2 h and washed as described above ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... 3 ug-mL-1 Human IgM (Innovative Research), a classical pathway activator ...
-
No products found
because this supplier's products are not listed.
Morten S. Hansen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... human GIP (0.1, 1, 10 and 100nM, Bachem), human GIP(3-30)NH2 (10µM ...
-
No products found
because this supplier's products are not listed.
Damian Dudka, R. Brian Akins, Michael A. Lampson,
bioRxiv - Cell Biology 2023
Quote:
... Centromeres were labeled with CREST (human anti-human Anti-Centromere Antibody, 1:200, Immunovision, HCT-0100) and an Alexa Fluor 594–conjugated goat anti-human secondary antibody (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Joan Sebastián Gallego-Murillo, et al.,
bioRxiv - Bioengineering 2022
Quote:
... human plasma-derived holotransferrin (1 mg/mL; Sanquin; Netherlands), and heparin (5 U/mL ...
-
No products found
because this supplier's products are not listed.
Jia Chen, et al.,
bioRxiv - Microbiology 2019
Quote:
... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
-
No products found
because this supplier's products are not listed.
Vinod Kumar, et al.,
bioRxiv - Neuroscience 2020
Quote:
... PSEN1dE9)85Dbo/Mmjax) expressing a chimeric mouse/human amyloid precursor protein and a mutant human presenilin 1 were source from Jackson Laboratory (RRID ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
Maike Pohlers, et al.,
bioRxiv - Cancer Biology 2023
Quote:
For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
-
No products found
because this supplier's products are not listed.
Ruud H. Wijdeven, et al.,
bioRxiv - Immunology 2021
Quote:
... HEK 293T cells were transfected using polyethyleneimine (Polyscience Inc.) with packaging plasmids pRSVrev ...
-
No products found
because this supplier's products are not listed.
Charlie J. Childs, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Human Fibrinogen 1 Plasminogen Depleted (Enzyme Research Lab Cat#FIB-1), and X-Vivo 20 (Lonza Cat#190995) ...
-
No products found
because this supplier's products are not listed.
Lindsey A Allan, et al.,
bioRxiv - Cell Biology 2019
Quote:
... human ACA (90C-CS1058 from Fitzgerald, 1:2000), rabbit Cyclin B1 (#12231S from Cell signalling technology ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
-
No products found
because this supplier's products are not listed.
Yu Zhang, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and rabbit-anti-human S100 (1:100, CP021A, Biocare) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Will Macnair, et al.,
bioRxiv - Neuroscience 2023
Quote:
... rabbit anti-human GPR17 (Cayman Chemical, 10136, 1:100), MBP (Rat anti MBP aa82-87 BioRad 1:300) ...
-
No products found
because this supplier's products are not listed.
Heng Lin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the immobilized FLAG-NBR1 was incubated with 1.5 μg His-MAPT and 1 μg GST or GST-BAG3 (Abnova H00009531-P01) in the BAG buffer (25 mM HEPES ...