-
No products found
because this supplier's products are not listed.
Lihong Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... and the RBD-mFc and NTD-mFc were purified using protein A agarose (ThermoFisher Scientific). In order to obtain RBD-SD1 and NTD ...
-
No products found
because this supplier's products are not listed.
Bert Schepens, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Human ACE-2-mFc protein (Sino Biological, Cat nr. 10108-H05H) was captured on anti-mouse IgG (Fc specific ...
-
No products found
because this supplier's products are not listed.
Filip Mihalic, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Melvin E. Klegerman, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 0.5 µg RBD-mFc (GenScript)/ml PBS-T was added to all wells and incubated for 2 hours ...
-
No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Ivan K. Domingo, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... HEK293-Null2 cells were also co-transfected with a human MD-2 expression clone (OriGene, RC204686). JetPRIME (Polyplus ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Cesar L. Moreno, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 (previously described20) were transduced with lentiviruses carrying dCAS9-10xGCN4 (Addgene 60903) termed HEK293-ACE2-SunTag ...
-
No products found
because this supplier's products are not listed.
Tomoyuki Nishikawa, et al.,
bioRxiv - Immunology 2021
Quote:
... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Pengfei Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
-
No products found
because this supplier's products are not listed.
K. Ehring, et al.,
bioRxiv - Cell Biology 2023
Quote:
... HEK293-derived human Shh (R&D Systems, 8908-SH) served as a bioactivity control and to quantify Bosc23-expressed ...
-
No products found
because this supplier's products are not listed.
Jillian M. DiMuzio, et al.,
bioRxiv - Immunology 2021
Quote:
... HEK293 cells expressing human Angiotensin converting enzyme 2 (ACE2) (BPS Biosciences, Cat #79951) were cultured in EMEM containing 10% FBS and 5 mg/mL Puromycin to select for ACE2-expressing cells ...
-
No products found
because this supplier's products are not listed.
Chloe G Myers, et al.,
bioRxiv - Cell Biology 2024
Quote:
Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
-
No products found
because this supplier's products are not listed.
Masako Kohyama, et al.,
bioRxiv - Microbiology 2022
Quote:
... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Warren W. Hom, et al.,
bioRxiv - Cell Biology 2024
Quote:
HEK293 cells expressing human ACVR1-GFP were stained with Lysopainter (Abcam, ab176829) and Hoechst (ThermoScientific ...
-
No products found
because this supplier's products are not listed.
Andy Q. Yuan, et al.,
bioRxiv - Immunology 2020
Quote:
... was incubated with 1-2 spike+ cells for 1 hour at 4°C before adding of 0.02 ug/ml ACE2-mFc followed by FITC conjugated anti-mouse IgG (Biolegend, cat#405305) and the MFI of FITC and positive percentage were collected.
-
No products found
because this supplier's products are not listed.
Chirag Vasavda, et al.,
bioRxiv - Neuroscience 2021
Quote:
HEK293 cells and MEFs were transiently transfected with PolyFect (Qiagen) and Lipofectamine 3000 (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Magdalena Skubal, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Cells cultured on the MFC were washed with PBS and fixed with fixation/permeabilization solution (BD Biosciences #554722) for 2h at room temperature with the syringe pump (2ul/min) ...
-
No products found
because this supplier's products are not listed.
Chiara Panzi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... then coverslips were mounted with Mowiol and MFCs with Ibidi mounting medium (ibidi #50001). Cells were imaged using a Zeiss LSM 510 or LSM 980 equipped with AiryScan2 ...
-
No products found
because this supplier's products are not listed.
Shengyun Ma, et al.,
bioRxiv - Immunology 2022
Quote:
... RT-PCR was performed on cDNAs from murine Th17 or human HEK293 cells using the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) with the standard thermocycling protocol ...
-
No products found
because this supplier's products are not listed.
Sara Cavallaro, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
-
No products found
because this supplier's products are not listed.
Saykat Das, et al.,
bioRxiv - Immunology 2023
Quote:
Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
-
No products found
because this supplier's products are not listed.
Xaria X. Li, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... HEK293 cells were transiently transfected with β-arrestin 2-Renilla luciferase 8 (Rluc8) and human C5aR2-Venus constructs using XTG9 (Roche, Sydney, Australia) for 24 h ...
-
No products found
because this supplier's products are not listed.
Adéla Tiffner, et al.,
bioRxiv - Biophysics 2020
Quote:
The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs) ...
-
No products found
because this supplier's products are not listed.
Julia Prigann, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
-
No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
-
No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... ACE2-mFc concentration was determined by sandwich ELISA using a goat anti-mouse IgG Fc (Jackson Immunoresearch, 115-006-071) for capture ...
-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Capria Rinaldi, et al.,
bioRxiv - Systems Biology 2022
Quote:
T-REx HEK293 cells were seeded in 384-well PhenoPlates (PerkinElmer #6057302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
-
No products found
because this supplier's products are not listed.
Ali Akbar Karkhaneh Yousefi, et al.,
bioRxiv - Biophysics 2023
Quote:
... using 10% Human Fn (Human Fn, Promocell) in PBS ...
-
No products found
because this supplier's products are not listed.
Bhagawat C. Subramanian, et al.,
bioRxiv - Cell Biology 2020
Quote:
... human C5a and human CXCL8 were from Santa Cruz Biotechnology Inc. ...
-
No products found
because this supplier's products are not listed.
Qing Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Human primary astrocytes (ScienCell) were cultivated as recommended by ScienCell ...
-
No products found
because this supplier's products are not listed.
Anthony A. Ruberto, et al.,
bioRxiv - Genomics 2021
Quote:
... primary human hepatocytes (BioIVT) were seeded 2-3 days prior to infection with 15,000-20,000 sporozoites per well ...
-
No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Funmilayo O. Fagbadebo, et al.,
bioRxiv - Cell Biology 2021
Quote:
... or HEK293-expressed GFP-Miro1 immobilized on ChromoTek GFP- Trap® Multiwell plate (Proteintech). To generate Nb-presenting phages ...
-
No products found
because this supplier's products are not listed.
Jelke J. Fros, et al.,
bioRxiv - Microbiology 2021
Quote:
... Human blood (Sanquin Blood Supply Foundation ...
-
No products found
because this supplier's products are not listed.
Denis D. Shi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... human albumin (Bethyl Laboratories ...
-
No products found
because this supplier's products are not listed.
David Mitchell III, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Libraries from randomly primed total RNA were prepared using the xGen NGS RNA (Integrated DNA Technologies) kit for HEK293 and the Nextera XT (Illumina) kit for E ...
-
No products found
Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
-
No products found
because this supplier's products are not listed.
Jonathan M. Goodwin, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were plated on 35 mm glass-bottomed dishes (Mattek, Ashland, MA). Images were acquired every 2 minutes using a spinning disk confocal microscope ...
-
No products found
because this supplier's products are not listed.
V. E. Dunlock, et al.,
bioRxiv - Immunology 2019
Quote:
... anti-human CD3 (OKT3, Bio X cell, anti-human CD28 (9.3 Bio X Cell), anti-human CD45 (REA747 ...