-
No products found
because this supplier's products are not listed.
Negin Khosraviani, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Human embryonic kidney 293 cells (HEK293T) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Wisent Bioproducts) supplemented with 10% (v/v ...
-
No products found
because this supplier's products are not listed.
Hugo G.J. Damstra, et al.,
bioRxiv - Cell Biology 2022
Quote:
... or 4-fold (R2-myc-his; a gift from S. Oliveira) molar excess of NHS-ester (ATTO 425, ATTO 647N; ATTO-TEC GmbH ...
-
No products found
because this supplier's products are not listed.
Anil Verma, et al.,
bioRxiv - Immunology 2023
Quote:
... were labeled with biotinylated anti-His tag monoclonal antibody (ThermoScientific) and used to capture His-tagged clade C gp120 Du151 protein (Immune Technologies). The gp120-expressing beads were then incubated with triplicate 5-fold dilutions of heat-inactivated serum samples in V-bottom plates for 1h at 37°C ...
-
No products found
because this supplier's products are not listed.
Michal Nemergut, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Data were collected at 293 K with a focused (Confocal Max-Flux, Rigaku) Cu Kα X-ray (1.54 Å) ...
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... 2 μg of the RBD-his mRNA or GFP mRNA (Trilink) was formulated in 6.6 μl of PBS pH 5.5 with 1.37 μl of 5mM CART and added to the cells ...
-
No products found
because this supplier's products are not listed.
Thomas Keating, et al.,
bioRxiv - Immunology 2021
Quote:
... IgG-depleted exogenous pooled human complement was pre-incubated for 20 minutes at 4°C with PBS (control) or mouse anti-human C1q mAb (Hycult Biotech, Netherlands) at 1/5 dilution ...
-
No products found
because this supplier's products are not listed.
Surbhi Verma, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human MDMs (hMDMs) were isolated from human PBMCs and separated from healthy human donors’ blood using PolymorphPrep (PROGEN,1895) layering and centrifugation ...
-
No products found
because this supplier's products are not listed.
Daisuke Ogasawara, et al.,
bioRxiv - Biochemistry 2024
Quote:
... N-term strep-His tag was first inserted into phCMV3 vector (Genlantis, cat # P003300) using Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
-
No products found
because this supplier's products are not listed.
Xiaoyu Shi, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and HEK 293T cells were seeded at 1 × 104 cells/cm2 in 16-well chambers (Grace Bio-Labs, 112358) and grown to 75% confluency.
-
No products found
because this supplier's products are not listed.
Alexa Schuettenberg, et al.,
bioRxiv - Immunology 2022
Quote:
... normal human IgG (ProSci #5503) on each plate as a positive control ...
-
No products found
because this supplier's products are not listed.
Peeali Mukherjee, et al.,
bioRxiv - Biophysics 2023
Quote:
... Crystals of FlrB-PAS7-108 were obtained by hanging drop vapour diffusion method at 293 K using 24 well crystallization tray with Ammonium Sulfate Grid Screen from Hampton Research with protein of concentration 10 mg ml-1 ...
-
No products found
because this supplier's products are not listed.
Marcel Rak, et al.,
bioRxiv - Biochemistry 2023
Quote:
Co-crystallization trials were performed using the sitting-drop vapor-diffusion method at 293 K with a mosquito crystallization robot (TTP Labtech, Royston UK). MST3 (11 mg/mL in 25 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Anna K. Hundsdoerfer, et al.,
bioRxiv - Genomics 2023
Quote:
... euphorbiae (from one head each; the same individuals used for the PacBio genome and Hi-C sequencing) and neural tissue from the internal reference standard Acheta domesticus (female ...
-
No products found
because this supplier's products are not listed.
Lior Tal, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Arabidopsis KAI2 protein was expressed as a 6× His-SUMO fusion protein using the expression vector pSUMO (LifeSensors). His-SUMO-KAI2 was isolated from by Ni-NTA resin and the eluted His-SUMO KAI2 was further separated by anion-exchange ...
-
No products found
because this supplier's products are not listed.
Thi K. Tran-Nguyen,
bioRxiv - Molecular Biology 2021
Quote:
Recombinant human GRP78s purchased from StressMarq Biosciences or produced in the laboratory [30] were used as antigens in ELISA ...
-
No products found
because this supplier's products are not listed.
M.A. Andres, et al.,
bioRxiv - Neuroscience 2022
Quote:
Human neural progenitor cells (hNPCs) (Neuromics, MN) were plated on Matrigel (Corning ...
-
No products found
because this supplier's products are not listed.
Alicja Przybyszewska-Podstawka, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... TGFβ (human TGFβ1, Biorbyt, Cambridge, United Kingdom), Gibson Assembly® Master Mix (NEB) ...
-
No products found
because this supplier's products are not listed.
Nazila V. Jafari, Jennifer L. Rohn,
bioRxiv - Microbiology 2022
Quote:
HBLAK human bladder progenitor cells (CELLnTEC, Switzerland) were grown according to the CELLnTEC protocol ...
-
No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
-
No products found
because this supplier's products are not listed.
Yunye Zhu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Transformants scraped from densely plated transformation plates were inoculated into fresh SC-His medium with 2% raffinose (Amresco, J392) at 0.25 x 107 cells/ml and grown until 0.5-0.8 x 107 cells/ml ...
-
No products found
because this supplier's products are not listed.
Maurizio Chioccioli, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... using human Col1a1 and Acta2 primers from ABI for the specified gene and species.
-
No products found
because this supplier's products are not listed.
Yuichi Mitsui, et al.,
bioRxiv - Immunology 2022
Quote:
Human PBMCs were purchased from Precision for Medicine, Inc ...
-
No products found
because this supplier's products are not listed.
G. Uppal, et al.,
bioRxiv - Biophysics 2020
Quote:
... The PEG-RGD and 4-arm PEG-acrylate (4-PEG-ACR, 20kDa, JenKem Technology) were mixed at a 1.5:8.5 (w/w ...
-
No products found
because this supplier's products are not listed.
Sherine E. Thomas, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Wizard 3&4 (Molecular Dimensions), JCSG +Suite (Molecular Dimensions) ...
-
No products found
because this supplier's products are not listed.
Lora Kovacheva, et al.,
bioRxiv - Neuroscience 2021
Quote:
... gabazine (SR95531, 4 µM; Biotrend) and DL-AP5 (10 µM ...
-
No products found
because this supplier's products are not listed.
Shiomi Junker, et al.,
bioRxiv - Microbiology 2024
Quote:
... The phosphorylation status of RsbV1/V2 was observed by running samples on SDS-PAGE gels (16% for His-V1/V2) supplemented with 20 µM of Phos binding reagent (APExBIO). Gels were stained with Coomassie brilliant blue and visualized with a Chemidoc Imager.
-
No products found
because this supplier's products are not listed.
Kawsar Hossain, et al.,
bioRxiv - Neuroscience 2024
Quote:
... incubated with EdU reaction solution (4 mM CuSO4, 4 µM Sulfo-Cyanine 3 Azide [Lumiprobe] ...
-
No products found
because this supplier's products are not listed.
Tommy Tong, et al.,
bioRxiv - Immunology 2023
Quote:
... followed by anti-human IgG AP conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT ...
-
No products found
because this supplier's products are not listed.
Siyuan Zhao, et al.,
bioRxiv - Neuroscience 2021
Quote:
... (4) Spin-coating LOR3A photoresist (MicroChem) at 4000 rpm ...
-
No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
-
No products found
because this supplier's products are not listed.
Lina Schiffer, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 11-ketoandrostenedione (4-androsten-3,11,17-trione) and 11-ketotestosterone (4-androsten-17β-ol-3,11-dione) were from Steraloids. The deuterated internal standards 11β-hydroxyandrostenedione-2,2,4,6,6,16,16-D7 (D7-11OHA4) ...
-
No products found
because this supplier's products are not listed.
Piyakarn Boontem, Tetsumori Yamashima,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... rabbit anti-human activated μ-calpain antibody (PEPTIDE Institute, Japan) at 1:250 overnight ...
-
No products found
because this supplier's products are not listed.
Yekyung Seong, et al.,
bioRxiv - Immunology 2021
Quote:
Anti-human CD11b (Clone M1/70, Leinco Technologies, Fenton, MO) was conjugated with Alexa Fluor 532 NHS ester according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Winifred M. Johnson, et al.,
bioRxiv - Microbiology 2021
Quote:
... 4-hydroxybenzoic acid-d4 from CDN Isotopes, and sodium taurocholate-d5 from Toronto Research Chemicals through Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Meng Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 12-port valves (IDEX, EZ1213-820-4) and a peristaltic pump (Gilson ...
-
No products found
because this supplier's products are not listed.
Bojana Radojevic, et al.,
bioRxiv - Cell Biology 2020
Quote:
Retinal organoids were fixed in 4% paraformaldehyde (FD neuroTechnologies) at room temperature (RT ...
-
No products found
because this supplier's products are not listed.
Liang Qu, et al.,
bioRxiv - Immunology 2022
Quote:
... and NHP IL-4 ELISpot assay kit (U-CyTech). The cryopreserved rhesus macaques PBMCs were thawed and cultured with pre-warmed AIM-V media ...
-
No products found
because this supplier's products are not listed.
Lingyun Zhao, Xiaoqin Wang,
bioRxiv - Neuroscience 2021
Quote:
... Tungsten electrodes (4-12MΩ, FHC or A-M Systems) housed in the arrays were advanced through the dura and adjusted in small steps (~50μm ...
-
No products found
because this supplier's products are not listed.
S. Karkampouna, et al.,
bioRxiv - Cell Biology 2021
Quote:
Mouse and human liver tissues were homogenised with UltraTurrax homogenizer (T25 basic, IKA) and TRIpure reagent (Roche) ...
-
No products found
because this supplier's products are not listed.
Caroline Atyeo, et al.,
bioRxiv - Immunology 2021
Quote:
... Avi-Tagged FcRs (Duke Human Vaccine Institute) were biotinylated using BirA500 kit (Avidity) per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jamie C Kwasnieski, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... 4 mM disulfide biotin azide (Click Chemistry Tools, Scottsdale, AZ) was added to a 10 μL reaction containing RNA ...
-
No products found
because this supplier's products are not listed.
Carissa L. Sirois, et al.,
bioRxiv - Genetics 2020
Quote:
... Gold enhancement was performed for 4 min using GoldEnhance (Nanoprobes). Cells were rinsed in deionized water to stop enhancement reaction.
-
No products found
because this supplier's products are not listed.
Fabio Henrique Brasil da Costa, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Tissue samples were decalcified using Formical-4™(StatLab, TX, USA), fixed with formalin and paraffin-embedded (FFPE) ...
-
No products found
because this supplier's products are not listed.
Giulia Ferrari, et al.,
bioRxiv - Cell Biology 2021
Quote:
... FACS data analysis was done using FCS Express 4 (De Novo Software). A similar procedure was followed for FACS cell purification ...
-
No products found
because this supplier's products are not listed.
Cerys S Manning, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... Western blots were performed using 4-20% Tris-glycine acrylamide gels (NuSep), Whatman Protran nitrocellulose membrane (Sigma ...
-
No products found
because this supplier's products are not listed.
M. Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
... at 4°C and loaded 3 times in a CellD disrupter (Constant Systems). The lysate was centrifuged for 15min at 12000 x g at 4°C to remove cell debris and the supernatant was centrifuged again for 1h at 100000 x g at 4°C ...
-
No products found
because this supplier's products are not listed.
Nicolas Gutierrez-Castellanos, et al.,
bioRxiv - Neuroscience 2023
Quote:
... N=4) at 27.6 nL/min using a nanoliter injector (Nanoject II, Drummond Scientific) at 0.1 Hz ...
-
No products found
because this supplier's products are not listed.
Susanne Neumann, et al.,
bioRxiv - Neuroscience 2024
Quote:
... The wound was sutured using Vicryl 4-0 (Ethicon, Johnson & Johnson, New Brunswick, NJ, USA) and the animals were placed on a heat pad until fully awake (∼30 min ...
-
No products found
because this supplier's products are not listed.
Govind Kunduri, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The cell suspension (50 ml) was sonicated at 4°C (Cole-Parmer ultrasonic processor model CP130,) with microprobe at an amplitude of 30 and on and off cycles of 10sec each for about 30 min to 1 hour ...
-
No products found
because this supplier's products are not listed.
David Alcantara-Gonzalez, et al.,
bioRxiv - Neuroscience 2024
Quote:
... followed by ∼20 mL of cold 4% PFA in 0.1 M PB using a peristaltic pump (Minipuls2, Gilson). The brain was quickly extracted and stored overnight in 4% PFA at 4°C ...